Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries

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Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries is …
instance of (P31):
scholarly articleQ13442814

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P6179Dimensions Publication ID1000268052
P356DOI10.1186/GB-2011-12-2-R18
P2888exact matchhttps://scigraph.springernature.com/pub.10.1186/gb-2011-12-2-r18
P8608Fatcat IDrelease_6ouremy7uzdixm2tdomtucg73y
P932PMC publication ID3188800
P698PubMed publication ID21338519
P5875ResearchGate publication ID49852627

P50authorChad NusbaumQ28036203
Andreas GnirkeQ29397647
P2093author name stringDavid B Jaffe
Timothy Fennell
Daniel Aird
Michael G Ross
Carsten Russ
Wei-Sheng Chen
Maxwell Danielsson
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P433issue2
P921main subjectbiasQ742736
P304page(s)R18
P577publication date2011-02-21
P1433published inGenome BiologyQ5533480
P1476titleAnalyzing and minimizing PCR amplification bias in Illumina sequencing libraries
P478volume12

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Q30417775Molecular diagnosis of autosomal dominant polycystic kidney disease using next-generation sequencing
Q59326781Monensin Alters the Functional and Metabolomic Profile of Rumen Microbiota in Beef Cattle
Q34204436Multi-platform assessment of transcriptome profiling using RNA-seq in the ABRF next-generation sequencing study.
Q35275073Multiplex single-molecule interaction profiling of DNA-barcoded proteins
Q34803562Multiplexed Illumina sequencing libraries from picogram quantities of DNA
Q56688655Multiplexed enrichment of rare DNA variants via sequence-selective and temperature-robust amplification
Q90240103Nanopore sequencing: An enrichment-free alternative to mitochondrial DNA sequencing
Q92162410Networks of mRNA Processing and Alternative Splicing Regulation in Health and Disease
Q46241596New specific primers for amplification of the Internal Transcribed Spacer region in Clitellata (Annelida).
Q52375104Next Generation Sequencing Methods for Diagnosis of Epilepsy Syndromes.
Q38456577Next Generation Sequencing-based analysis of RNA polymerase functions
Q38014423Next generation quantitative genetics in plants
Q34077829Next generation sequencing-based analysis of repetitive DNA in the model dioecious [corrected] plant Silene latifolia
Q90212657Next-generation forward genetic screens: using simulated data to improve the design of mapping-by-sequencing experiments in Arabidopsis
Q45999197Next-generation sequencing and phylogenetic signal of complete mitochondrial genomes for resolving the evolutionary history of leaf-nosed bats (Phyllostomidae).
Q35199471Next-generation sequencing fragment library construction
Q57801296Noise cancellation using total variation for copy number variation detection
Q55334173Non-biological synthetic spike-in controls and the AMPtk software pipeline improve mycobiome data.
Q54184929Non-invasive prenatal diagnostic testing for β-thalassaemia using cell-free fetal DNA and next generation sequencing.
Q50051267Non-invasive prenatal testing for fetal inheritance of maternal β-thalassaemia mutations using targeted sequencing and relative mutation dosage: a feasibility study.
Q36392412Non-random fragmentation patterns in circulating cell-free DNA reflect epigenetic regulation
Q34147576Noncoding RNAs as emerging regulators of Plasmodium falciparum virulence gene expression.
Q34496747Noninvasive Fetal Trisomy (NIFTY) test: an advanced noninvasive prenatal diagnosis methodology for fetal autosomal and sex chromosomal aneuploidies
Q35930225Normalization, bias correction, and peak calling for ChIP-seq
Q33686861Novel Algorithms for Improved Sensitivity in Non-Invasive Prenatal Testing
Q33678448Novel metrics to measure coverage in whole exome sequencing datasets reveal local and global non-uniformity.
Q35754535Nucleosome Organization in Human Embryonic Stem Cells
Q33768883OccuPeak: ChIP-Seq peak calling based on internal background modelling
Q38053654Ocean viruses: rigorously evaluating the metagenomic sample-to-sequence pipeline.
Q28646132Odintifier--A computational method for identifying insertions of organellar origin from modern and ancient high-throughput sequencing data based on haplotype phasing
Q50033786Optimal enzymes for amplifying sequencing libraries.
Q36407303Optimization of design and production strategies for novel adeno-associated viral display peptide libraries
Q30383764Optimized Illumina PCR-free library preparation for bacterial whole genome sequencing and analysis of factors influencing de novo assembly
Q31076879Optimizing Illumina next-generation sequencing library preparation for extremely AT-biased genomes.
Q34902839Optimizing information in Next-Generation-Sequencing (NGS) reads for improving de novo genome assembly
Q36050120PCR Strategies for Complete Allele Calling in Multigene Families Using High-Throughput Sequencing Approaches
Q89688947PCR inhibition in qPCR, dPCR and MPS-mechanisms and solutions
Q90095467PCR-free whole exome sequencing: Cost-effective and efficient in detecting rare mutations
Q57274915PM-Seq: Using Finite Poisson Mixture Models for RNA-Seq Data Analysis and Transcript Expression Level Quantification
Q30611167PRICE: software for the targeted assembly of components of (Meta) genomic sequence data
Q34338922Paired-end sequencing of Fosmid libraries by Illumina
Q30953170PathoQC: Computationally Efficient Read Preprocessing and Quality Control for High-Throughput Sequencing Data Sets
Q30378821Pattern Recognition on Read Positioning in Next Generation Sequencing.
Q33805165Patterns of sequencing coverage bias revealed by ultra-deep sequencing of vertebrate mitochondria
Q38387997PeakXus: comprehensive transcription factor binding site discovery from ChIP-Nexus and ChIP-Exo experiments.
Q35126522Performance comparison between rapid sequencing platforms for ultra-low coverage sequencing strategy
Q34023426Performance comparison of exome DNA sequencing technologies
Q35184230Performance comparison of four exome capture systems for deep sequencing
Q36896837Performance of 16s rDNA Primer Pairs in the Study of Rhizosphere and Endosphere Bacterial Microbiomes in Metabarcoding Studies
Q33359151Piecing the puzzle together: a revisit to transcript reconstruction problem in RNA-seq
Q88423042Polyamine Control of Translation Elongation Regulates Start Site Selection on Antizyme Inhibitor mRNA via Ribosome Queuing
Q93115146Population Genome Sequencing of the Scab Fungal Species Venturia inaequalis, Venturia pirina, Venturia aucupariae and Venturia asperata
Q36532705Precision methylome characterization of Mycobacterium tuberculosis complex (MTBC) using PacBio single-molecule real-time (SMRT) technology
Q30743735Prevention, diagnosis and treatment of high-throughput sequencing data pathologies
Q51518031Probabilistic modeling of Hi-C contact maps eliminates systematic biases to characterize global chromosomal architecture.
Q34195606Producing parasitic helminth reference and draft genomes at the Wellcome Trust Sanger Institute.
Q46724083Progressive retinal atrophy in the Polski Owczarek Nizinny dog: a clinical and genetic study
Q49468107Proper experimental design requires randomization/balancing of molecular ecology experiments.
Q28084330Protein-DNA binding in high-resolution
Q36214540Q-RRBS: a quantitative reduced representation bisulfite sequencing method for single-cell methylome analyses.
Q33663633Quality control on the frontier
Q46063281Quantification of Inter-Sample Differences in T-Cell Receptor Repertoires Using Sequence-Based Information
Q36051182Quantitation of next generation sequencing library preparation protocol efficiencies using droplet digital PCR assays - a systematic comparison of DNA library preparation kits for Illumina sequencing
Q34530591Quantitative assessment of mitochondrial DNA copies from whole genome sequencing
Q35579082RNA sequencing for transcript 5'-end mapping in mycobacteria
Q35541156RNA-Rocket: an RNA-Seq analysis resource for infectious disease research
Q34284546RNA-Seq and human complex diseases: recent accomplishments and future perspectives.
Q64091356RNA-sequencing in ophthalmology research: considerations for experimental design and analysis
Q28607995Rare variant discovery by deep whole-genome sequencing of 1,070 Japanese individuals
Q55091835Reassembling haplotypes in a mixture of pooled amplicons when the relative concentrations are known: A proof-of-concept study on the efficient design of next-generation sequencing strategies.
Q35856749Reassessment of the Genome Size in Elaeis guineensis and Elaeis oleifera, and Its Interspecific Hybrid
Q48105064Recent Advances in Detecting Mitochondrial DNA Heteroplasmic Variations.
Q47558038Recent insights into the tick microbiome gained through next-generation sequencing
Q34143874Removing technical variability in RNA-seq data using conditional quantile normalization
Q31027821Replicate exome-sequencing in a multiple-generation family: improved interpretation of next-generation sequencing data
Q42774137Reproducibility of high-throughput mRNA and small RNA sequencing across laboratories
Q53276840Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens.
Q89582757Robust RNA-Seq of aRNA-amplified single cell material collected by patch clamp
Q49718011Rolling circle amplification shows a sinusoidal template length-dependent amplification bias.
Q33557378SPlinted Ligation Adapter Tagging (SPLAT), a novel library preparation method for whole genome bisulphite sequencing
Q92752880Screening and evaluating of long non-coding RNAs in prenatal and postnatal pituitary gland of sheep
Q36049287Sequence Capture versus Restriction Site Associated DNA Sequencing for Shallow Systematics
Q36765190Sequence features accurately predict genome-wide MeCP2 binding in vivo.
Q90698816Sequence properties of certain GC rich avian genes, their origins and absence from genome assemblies: case studies
Q34398440Sequencing depth and coverage: key considerations in genomic analyses
Q38816948Sequencing on the SOLiD 5500xl System - in-depth characterization of the GC bias
Q30454551Sequencing platform and library preparation choices impact viral metagenomes
Q30455439Shining a light on dark sequencing: characterising errors in Ion Torrent PGM data
Q22003039Shotgun metagenomes and multiple primer pair-barcode combinations of amplicons reveal biases in metabarcoding analyses of fungi
Q47095620Single molecule sequencing of the M13 virus genome without amplification
Q36914760Single molecule targeted sequencing for cancer gene mutation detection
Q34369763Single nucleotide analysis of cytosine methylation by whole-genome shotgun bisulfite sequencing
Q57897811Single-cell genomics: An overview
Q64089553Single-cell transcriptome analysis of Physcomitrella leaf cells during reprogramming using microcapillary manipulation
Q47447856Size-selective DNA separation: recovery spectra help determine the sodium chloride (NaCl) and polyethylene glycol (PEG) concentrations required
Q28296073Small, smaller, smallest: the origins and evolution of ancient dual symbioses in a Phloem-feeding insect
Q58116117Solid-phase enzyme catalysis of DNA end repair and 3' A-tailing reduces GC-bias in next-generation sequencing of human genomic DNA
Q33822089Sources and Functions of Extracellular Small RNAs in Human Circulation
Q30981347Sources of PCR-induced distortions in high-throughput sequencing data sets
Q39748197Spatial distribution of aquatic marine fungi across the western Arctic and sub-arctic.
Q94960300Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays
Q30575346Special features of RAD Sequencing data: implications for genotyping
Q56980480Standard operating procedure for somatic variant refinement of sequencing data with paired tumor and normal samples
Q38040034Statistical challenges associated with detecting copy number variations with next-generation sequencing.
Q35662591Strand-specific RNA sequencing in Plasmodium falciparum malaria identifies developmentally regulated long non-coding RNA and circular RNA.
Q38296746Strategies for validation and testing of DNA methylation biomarkers.
Q35142841Strengths and limitations of 16S rRNA gene amplicon sequencing in revealing temporal microbial community dynamics.
Q57045853Succession of picophytoplankton during the spring bloom 2012 in Disko Bay (West Greenland)—an unexpectedly low abundance of green algae
Q28728245Summarizing and correcting the GC content bias in high-throughput sequencing
Q28647332Swabs to genomes: a comprehensive workflow
Q49911969Systematic and stochastic influences on the performance of the MinION nanopore sequencer across a range of nucleotide bias.
Q91968519Systematic evaluation of library preparation methods and sequencing platforms for high-throughput whole genome bisulfite sequencing
Q36084768Systematic improvement of amplicon marker gene methods for increased accuracy in microbiome studies
Q35826318TARDIS, a targeted RNA directional sequencing method for rare RNA discovery
Q53280647TRUmiCount: Correctly counting absolute numbers of molecules using unique molecular identifiers.
Q38630683Talking the talk, but not walking the walk: RT-qPCR as a paradigm for the lack of reproducibility in molecular research
Q34035161Targeted high throughput sequencing in clinical cancer settings: formaldehyde fixed-paraffin embedded (FFPE) tumor tissues, input amount and tumor heterogeneity
Q36404734Targeted sequencing of both DNA strands barcoded and captured individually by RNA probes to identify genome-wide ultra-rare mutations
Q41674738Technical Advances in the Measurement of Residual Disease in Acute Myeloid Leukemia.
Q35060890Testing the reproducibility of multiple displacement amplification on genomes of clonal endosymbiont populations
Q91045004The Personal Genome Project-UK, an open access resource of human multi-omics data
Q89666114The Progress of Multi-Omics Technologies: Determining Function in Lactic Acid Bacteria Using a Systems Level Approach
Q104608401The RNA Architecture of the SARS-CoV-2 3'-Untranslated Region
Q92821693The Tempo and Mode of Angiosperm Mitochondrial Genome Divergence Inferred from Intraspecific Variation in Arabidopsis thaliana
Q27313398The ability of inner-cell-mass cells to self-renew as embryonic stem cells is acquired following epiblast specification
Q38707847The draft genome sequence of Hymenobacter sp. CRA2 isolated from Nama Karoo shrub land soils from South Africa.
Q35346690The effects of variable sample biomass on comparative metagenomics
Q38058682The expanding scope of DNA sequencing
Q21560744The fast changing landscape of sequencing technologies and their impact on microbial genome assemblies and annotation
Q36292756The impact of RNA secondary structure on read start locations on the Illumina sequencing platform
Q46306438The microbiome associated with two Synechococcus ribotypes at different levels of ecological interaction.
Q38983137The mitochondrial genome of the lone star tick (Amblyomma americanum).
Q38811527The present and future of de novo whole-genome assembly
Q34325516The rhoptry proteins ROP18 and ROP5 mediate Toxoplasma gondii evasion of the murine, but not the human, interferon-gamma response.
Q97072562The role of Long Non-Coding RNAs (lncRNAs) and downstream signaling pathways in Leukemia progression
Q33921022The role of replicates for error mitigation in next-generation sequencing
Q28298852The statistical-mechanics of chromosome conformation capture
Q36947263The use of RelocaTE and unassembled short reads to produce high-resolution snapshots of transposable element generated diversity in rice
Q34008445Titration-free 454 sequencing using Y adapters
Q58553934Toward Integrative Bacterial Monitoring of Metolachlor Toxicity in Groundwater
Q39157662Toward a Resilient, Functional Microbiome: Drought Tolerance-Alleviating Microbes for Sustainable Agriculture
Q30363613Toward reliable biomarker signatures in the age of liquid biopsies - how to standardize the small RNA-Seq workflow
Q34196783Towards accurate characterization of clonal heterogeneity based on structural variation
Q28771734Translation of 5' leaders is pervasive in genes resistant to eIF2 repression
Q34007229Transposable element dynamics among asymbiotic and ectomycorrhizal Amanita fungi.
Q38382209UMI-tools: modeling sequencing errors in Unique Molecular Identifiers to improve quantification accuracy
Q34557106Understanding nucleosome dynamics and their links to gene expression and DNA replication
Q28647858Understanding spatial organizations of chromosomes via statistical analysis of Hi-C data
Q36997969User guide for mapping-by-sequencing in Arabidopsis
Q41845857Using false discovery rates to benchmark SNP-callers in next-generation sequencing projects
Q45823548Validation of a next-generation sequencing assay for clinical molecular oncology.
Q48512286Variable rates of simple satellite gains across the Drosophila phylogeny.
Q35209205Variant detection sensitivity and biases in whole genome and exome sequencing
Q27016083Viral surveillance and discovery
Q47286390WISExome: a within-sample comparison approach to detect copy number variations in whole exome sequencing data
Q36129644Whole Genome Sequencing of Enterovirus species C Isolates by High-Throughput Sequencing: Development of Generic Primers.
Q57667365Whole exome sequencing is an efficient and sensitive method for detection of germline mutations in patients with phaeochromcytomas and paragangliomas
Q38651500Whole-genome sequencing approaches for conservation biology: advantages, limitations, and practical recommendations.
Q36013634Whole-genome sequencing overcomes pseudogene homology to diagnose autosomal dominant polycystic kidney disease
Q33954128Whole-transcriptome RNAseq analysis from minute amount of total RNA.
Q33565173Why assembling plant genome sequences is so challenging
Q58553596omniCLIP: probabilistic identification of protein-RNA interactions from CLIP-seq data
Q43759417pIRS: Profile-based Illumina pair-end reads simulator
Q38396444proovread: large-scale high-accuracy PacBio correction through iterative short read consensus
Q64069742scruff: an R/Bioconductor package for preprocessing single-cell RNA-sequencing data
Q116675556Genome-wide survey reveals the phylogenomic relationships of Chirolophis japonicus Herzenstein, 1890 (Stichaeidae, Perciformes)

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