Identification of multiple rate-limiting steps during the human mitochondrial transcription cycle in vitro

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Identification of multiple rate-limiting steps during the human mitochondrial transcription cycle in vitro is …
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scholarly articleQ13442814

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P356DOI10.1074/JBC.M109.092676
P932PMC publication ID2871506
P698PubMed publication ID20351113

P50authorCraig E. CameronQ74809374
P2093author name stringJamie J Arnold
Akira U Uchida
Ibrahim M Moustafa
Maria F Lodeiro
Shelley L Reynolds
P2860cites workHuman mitochondrial ribosomal protein MRPL12 interacts directly with mitochondrial RNA polymerase to modulate mitochondrial gene expressionQ24299068
Mitochondrial transcription factors B1 and B2 activate transcription of human mtDNAQ24299860
Structural analysis and DNA binding of the HMG domains of the human mitochondrial transcription factor AQ24308719
Human mitochondrial transcription factor B1 interacts with the C-terminal activation region of h-mtTFA and stimulates transcription independently of its RNA methyltransferase activityQ24313359
A human mitochondrial transcription factor is related to RNA adenine methyltransferases and binds S-adenosylmethionineQ24537551
The mitochondrial RNA polymerase contributes critically to promoter specificity in mammalian cellsQ24559899
Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templatesQ24632342
Direct detection of abortive RNA transcripts in vivoQ24641939
Initial transcription by RNA polymerase proceeds through a DNA-scrunching mechanismQ24643943
Structural basis of transcription: an RNA polymerase II elongation complex at 3.3 A resolutionQ27631280
HMG1 and 2, and related 'architectural' DNA-binding proteinsQ28204610
Mitochondrial disorders in the nervous systemQ28272320
Similarity of human mitochondrial transcription factor 1 to high mobility group proteinsQ28278034
Promoter selection in human mitochondria involves binding of a transcription factor to orientation-independent upstream regulatory elementsQ28611665
The transition from transcriptional initiation to elongationQ29035794
A mitochondrial paradigm of metabolic and degenerative diseases, aging, and cancer: a dawn for evolutionary medicineQ29547303
Studies of contacts between T7 RNA polymerase and its promoter reveal features in common with multisubunit RNA polymerasesQ30673017
TFB2 is a transient component of the catalytic site of the human mitochondrial RNA polymeraseQ33589922
Defining mechanisms that regulate RNA polymerase II transcription in vivoQ33708432
The effects of upstream DNA on open complex formation by Escherichia coli RNA polymeraseQ33722783
Transcription and replication of mitochondrial DNA.Q34066051
Processivity in early stages of transcription by T7 RNA polymeraseQ34178306
Bacterial RNA polymerases: the wholo storyQ34531247
Inherited mitochondrial diseases of DNA replicationQ34692482
Methylation of 12S rRNA is necessary for in vivo stability of the small subunit of the mammalian mitochondrial ribosomeQ34974795
Replication and transcription of mammalian mitochondrial DNA.Q35044272
Coupling the mitochondrial transcription machinery to human diseaseQ35885709
The regulatory roles and mechanism of transcriptional pausingQ36638927
Initiation and beyond: multiple functions of the human mitochondrial transcription machineryQ36692560
Mitochondria as chi.Q36724311
Mitochondrial transcription and its regulation in mammalian cellsQ36732857
DNA replication and transcription in mammalian mitochondriaQ36780113
Purification and characterization of human mitochondrial transcription factor 1.Q36793853
Why do we still have a maternally inherited mitochondrial DNA? Insights from evolutionary medicineQ36824047
Nam1p, a protein involved in RNA processing and translation, is coupled to transcription through an interaction with yeast mitochondrial RNA polymeraseQ37021667
Mitochondrial mutations: genotype to phenotype.Q37030151
Allosteric control of Escherichia coli rRNA promoter complexes by DksA.Q37111105
Poised polymerases: on your mark...get set...go!Q37136094
Mechanism of sequence-specific pausing of bacterial RNA polymeraseQ37211398
Promoter proximal pausing on genes in metazoansQ37284766
DNA polymerase gamma and mitochondrial disease: understanding the consequence of POLG mutations.Q37325936
RNA polymerase active center: the molecular engine of transcriptionQ37505069
Divergent transcription: a new feature of active promotersQ37549811
Binding of the priming nucleotide in the initiation of transcription by T7 RNA polymeraseQ38361121
Mitochondrial transcription factor B2 is essential for metabolic function in Drosophila melanogaster developmentQ39259724
Drosophila mitochondrial transcription factor B1 modulates mitochondrial translation but not transcription or DNA copy number in Schneider cells.Q40450206
Drosophila mitochondrial transcription factor B2 regulates mitochondrial DNA copy number and transcription in schneider cellsQ40570833
Molecular genetic aspects of human mitochondrial disordersQ41000631
Low levels of mitochondrial transcription factor A in mitochondrial DNA depletionQ41467609
Analysis of open complex formation during RNA polymerase II transcription initiation using heteroduplex templates and potassium permanganate probingQ41544294
Basic mechanisms of transcript elongation and its regulationQ41550178
Fluorescence mapping of the open complex of yeast mitochondrial RNA polymeraseQ42030855
A promoter recognition mechanism common to yeast mitochondrial and phage t7 RNA polymerasesQ42125728
Kinetic and thermodynamic basis of promoter strength: multiple steps of transcription initiation by T7 RNA polymerase are modulated by the promoter sequenceQ43912691
Markedly different ATP requirements for rRNA synthesis and mtDNA light strand transcription versus mRNA synthesis in isolated human mitochondria.Q44065396
The energetics of consensus promoter opening by T7 RNA polymeraseQ44209020
In vitro studies of transcript initiation by Escherichia coli RNA polymerase. 1. RNA chain initiation, abortive initiation, and promoter escape at three bacteriophage promotersQ44384188
Kinetic analysis of T7 RNA polymerase-promoter interactions with small synthetic promotersQ45152622
Flexible recognition of rapidly evolving promoter sequences by mitochondrial transcription factor 1.Q46424856
Mitochondrial transcription factor A regulates mtDNA copy number in mammalsQ47963219
Addition of a 29 residue carboxyl-terminal tail converts a simple HMG box-containing protein into a transcriptional activator.Q52208302
Mechanism of instability in abortive cycling by T7 RNA polymerase.Q54462885
Abortive Cycling and the Release of Polymerase for Elongation at the σ54-dependent glnAp2 PromoterQ54601725
Functional Transcription Elongation Complexes from Synthetic RNA-DNA Bubble DuplexesQ54668743
T7 RNA polymerase does not interact with the 5'-phosphate of the initiating nucleotide.Q54731392
Urinary oestrogen patterns in long follicular phasesQ57420062
Roles for a promoter and RNA processing in the synthesis of mitochondrial displacement-loop strandsQ69052240
Hierarchy of base-pair preference in the binding domain of the bacteriophage T7 promoterQ70591295
Equilibrium and stopped-flow kinetic studies of interaction between T7 RNA polymerase and its promoters measured by protein and 2-aminopurine fluorescence changesQ71825297
Termination and slippage by bacteriophage T7 RNA polymeraseQ72922801
Single-nucleotide resolution of RNA strands in the presence of their RNA complementsQ72998260
Role of open complex instability in kinetic promoter selection by bacteriophage T7 RNA polymeraseQ73876669
Poliovirus RNA-dependent RNA polymerase (3Dpol) is sufficient for template switching in vitroQ77896816
Sensitivity of the yeast mitochondrial RNA polymerase to +1 and +2 initiating nucleotidesQ79142892
Conserved sequence box II directs transcription termination and primer formation in mitochondriaQ79766217
Termination factor-mediated DNA loop between termination and initiation sites drives mitochondrial rRNA synthesisQ82081316
Mitochondrial transcription is regulated via an ATP "sensing" mechanism that couples RNA abundance to respirationQ83294912
P4510describes a project that usesImageQuantQ112270642
P433issue21
P407language of work or nameEnglishQ1860
P1104number of pages16
P304page(s)16387-16402
P577publication date2010-03-29
P1433published inJournal of Biological ChemistryQ867727
P1476titleIdentification of multiple rate-limiting steps during the human mitochondrial transcription cycle in vitro
P478volume285

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cites work (P2860)
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Q33982027Core human mitochondrial transcription apparatus is a regulated two-component system in vitro.
Q38366265Expression and Purification of Mitochondrial RNA Polymerase and Transcription Factor A from Drosophila melanogaster
Q36280608Human Cockayne syndrome B protein reciprocally communicates with mitochondrial proteins and promotes transcriptional elongation
Q36972846Human mitochondrial RNA polymerase: evaluation of the single-nucleotide-addition cycle on synthetic RNA/DNA scaffolds
Q39034736Inhibition of hepatitis C virus replication by GS-6620, a potent C-nucleoside monophosphate prodrug.
Q27001150Mitochondrial DNA damage and its consequences for mitochondrial gene expression
Q35518798Mitochondrial ribosomal protein L12 selectively associates with human mitochondrial RNA polymerase to activate transcription
Q38798497Mitochondrial transcription in mammalian cells
Q90443912Roles of the mitochondrial genetics in cancer metastasis: not to be ignored any longer
Q28485227Sensitivity of mitochondrial transcription and resistance of RNA polymerase II dependent nuclear transcription to antiviral ribonucleosides
Q49185722Simple In Vitro Assay To Evaluate the Incorporation Efficiency of Ribonucleotide Analog 5'-Triphosphates into RNA by Human Mitochondrial DNA-Dependent RNA Polymerase.
Q24294939The mitochondrial transcription and packaging factor Tfam imposes a U-turn on mitochondrial DNA
Q34516555Therapeutic efficacy of the small molecule GS-5734 against Ebola virus in rhesus monkeys
Q47906191Topological requirements of the mitochondrial heavy-strand promoters.
Q28730231Transcription from the second heavy-strand promoter of human mtDNA is repressed by transcription factor A in vitro
Q40395987Transcriptional activation by mitochondrial transcription factor A involves preferential distortion of promoter DNA.
Q35922066Transcriptional requirements of the distal heavy-strand promoter of mtDNA.
Q41348904Unexpected sequences and structures of mtDNA required for efficient transcription from the first heavy-strand promoter.

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