Key amino acid residues involved in multi-point binding interactions between brazzein, a sweet protein, and the T1R2-T1R3 human sweet receptor

scientific article

Key amino acid residues involved in multi-point binding interactions between brazzein, a sweet protein, and the T1R2-T1R3 human sweet receptor is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1016/J.JMB.2010.03.017
P932PMC publication ID2879441
P698PubMed publication ID20302879
P5875ResearchGate publication ID42369840

P50authorJohn MarkleyQ29946263
Fariba M Assadi-PorterQ61471818
P2093author name stringJames T Radek
Jeniffer Quijada
Marianna Max
Emeline L Maillet
P2860cites workThe cysteine-rich region of T1R3 determines responses to intensely sweet proteinsQ24300689
Different functional roles of T1R subunits in the heteromeric taste receptorsQ24564155
Structures of the extracellular regions of the group II/III metabotropic glutamate receptorsQ24681315
Crystal structure of rhodopsin: A G protein-coupled receptorQ27625972
Structural basis of glutamate recognition by a dimeric metabotropic glutamate receptorQ27627995
Structural views of the ligand-binding cores of a metabotropic glutamate receptor complexed with an antagonist and both glutamate and Gd3+.Q27638103
The mechanism of interaction of sweet proteins with the T1R2-T1R3 receptor: evidence from the solution structure of G16A-MNEIQ27641034
Curculin exhibits sweet-tasting and taste-modifying activities through its distinct molecular surfacesQ27648509
Solution structure of the thermostable sweet-tasting protein brazzeinQ27758760
Efficient production of recombinant brazzein, a small, heat-stable, sweet-tasting protein of plant originQ28142579
Sweetness determinant sites of brazzein, a small, heat-stable, sweet-tasting proteinQ28142582
Brazzein, a new high-potency thermostable sweet protein from Pentadiplandra brazzeana BQ28240451
Interaction of sweet proteins with their receptor. A conformational study of peptides corresponding to loops of brazzein, monellin and thaumatinQ28262549
From small sweeteners to sweet proteins: anatomy of the binding sites of the human T1R2_T1R3 receptorQ81077642
Distinct contributions of T1R2 and T1R3 taste receptor subunits to the detection of sweet stimuliQ81446267
Synthesis and characterization of the sweet protein brazzeinQ28297241
Functional interaction between T2R taste receptors and G-protein alpha subunits expressed in taste receptor cellsQ28566138
Mammalian sweet taste receptorsQ29614809
Critical regions for the sweetness of brazzeinQ30940898
Determination of disulfide array and subunit structure of taste-modifying protein, miraculinQ33396846
Interactions between the human sweet-sensing T1R2-T1R3 receptor and sweeteners detected by saturation transfer difference NMR spectroscopyQ33621471
Structures of heat-stable and unstable homologues of the sweet protein mabinlin. The difference in the heat stability is due to replacement of a single amino acid residueQ34060162
Lactisole interacts with the transmembrane domains of human T1R3 to inhibit sweet taste.Q34387670
Neoculin, a taste-modifying protein, is recognized by human sweet taste receptorQ34570564
Taste-modifying sweet protein, neoculin, is received at human T1R3 amino terminal domainQ34627992
Purification of monellin, the sweet principle of Dioscoreophyllum cumminsii.Q34701149
Structure of the metabotropic glutamate receptorQ35172787
Activation mechanism of the heterodimeric GABA(B) receptorQ35903180
Brazzein, a small, sweet protein: effects of mutations on its structure, dynamics and functional propertiesQ36058046
Natural sweet macromolecules: how sweet proteins workQ36523988
Efficient and rapid protein expression and purification of small high disulfide containing sweet protein brazzein in E. coliQ36642541
The heterodimeric sweet taste receptor has multiple potential ligand binding sites.Q36683638
Evaluating the 'Labeled Magnitude Scale' for measuring sensations of taste and smellQ38460105
Activation of a dimeric metabotropic glutamate receptor by intersubunit rearrangement.Q40081094
Molecular mechanisms of sweet receptor function.Q40452610
Closed state of both binding domains of homodimeric mGlu receptors is required for full activity.Q40538867
Interactions of the sweet protein brazzein with the sweet taste receptorQ42424760
Crystal structure of neoculin: insights into its sweetness and taste-modifying activity.Q42683280
Sweetness and enzymatic activity of lysozymeQ43765964
Why are sweet proteins sweet? Interaction of brazzein, monellin and thaumatin with the T1R2-T1R3 receptorQ44123505
Structural determination of the active site of a sweet protein. A 1H NMR investigation of pMNEI.Q44135566
Correlation of the Sweetness of Variants of the Protein Brazzein with Patterns of Hydrogen Bonds Detected by NMR SpectroscopyQ47931583
Measurement of protein by spectrophotometry at 205 nm.Q52909585
The Importance of Electrostatic Potential in The Interaction of Sweet Proteins with the Sweet Taste ReceptorQ57951881
13C NMR chemical shifts can predict disulfide bond formationQ73255305
Monkey electrophysiological and human psychophysical responses to mutants of the sweet protein brazzein: delineating brazzein sweetnessQ73784307
Isolation and characterization of the sweet principle from Dioscoreophyllum cumminsii (stapf) DielsQ77919270
Riboflavin-binding protein exhibits selective sweet suppression toward protein sweetenersQ79431522
P433issue4
P407language of work or nameEnglishQ1860
P304page(s)584-599
P577publication date2010-03-17
P1433published inJournal of Molecular BiologyQ925779
P1476titleKey amino acid residues involved in multi-point binding interactions between brazzein, a sweet protein, and the T1R2-T1R3 human sweet receptor
P478volume398

Reverse relations

cites work (P2860)
Q36536317A Hypersweet Protein: Removal of The Specific Negative Charge at Asp21 Enhances Thaumatin Sweetness
Q38849704A review of the associations between single nucleotide polymorphisms in taste receptors, eating behaviors, and health.
Q36363428Characterization of the Binding Site of Aspartame in the Human Sweet Taste Receptor
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Q38936327Convergent evolution of defensin sequence, structure and function
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Q50325158Positive Charges on the Surface of Thaumatin Are Crucial for the Multi-Point Interaction with the Sweet Receptor
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Q27670834Structural Role of the Terminal Disulfide Bond in the Sweetness of Brazzein
Q36749703Structure-function relationships of brazzein variants with altered interactions with the human sweet taste receptor
Q38043944Sweet-taste-suppressing compounds: current knowledge and perspectives of application
Q38706091Taste buds: cells, signals and synapses
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Q47952495The cephalic phase insulin response to nutritive and low-calorie sweeteners in solid and beverage form.
Q30401596The impact of Thr91 mutation on c-Src resistance to UM-164: molecular dynamics study revealed a new opportunity for drug design
Q27684254The structure of brazzein, a sweet-tasting protein from the wild African plant Pentadiplandra brazzeana
Q37135922Use of NMR saturation transfer difference spectroscopy to study ligand binding to membrane proteins

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