A high-throughput screening fluorescence polarization assay for fatty acid adenylating enzymes in Mycobacterium tuberculosis

scientific article

A high-throughput screening fluorescence polarization assay for fatty acid adenylating enzymes in Mycobacterium tuberculosis is …
instance of (P31):
scholarly articleQ13442814

External links are
P356DOI10.1016/J.AB.2011.06.037
P932PMC publication ID3152590
P698PubMed publication ID21771578
P5875ResearchGate publication ID51504480

P2093author name stringCourtney C Aldrich
Kimberly D Grimes
P2860cites workA fluorescence polarization based Src-SH2 binding assayQ36857122
Aryl acid adenylating enzymes involved in siderophore biosynthesis: fluorescence polarization assay, ligand specificity, and discovery of non-nucleoside inhibitors via high-throughput screening.Q37125144
Displacement of protein-bound aptamers with small molecules screened by fluorescence polarizationQ38292056
Regulation of expression of mas and fadD28, two genes involved in production of dimycocerosyl phthiocerol, a virulence factor of Mycobacterium tuberculosisQ39680917
Mycobacterial cell wall: structure and role in natural resistance to antibioticsQ40648841
Drug sensitivity and environmental adaptation of mycobacterial cell wall components.Q41129978
A detergent-based assay for the detection of promiscuous inhibitorsQ42007896
A continuous kinetic assay for adenylation enzyme activity and inhibitionQ42076043
The Pks13/FadD32 crosstalk for the biosynthesis of mycolic acids in Mycobacterium tuberculosisQ42097475
2,8-Disubstituted adenosine derivatives as partial agonists for the adenosine A2A receptorQ44416595
The dual function of the Mycobacterium tuberculosis FadD32 required for mycolic acid biosynthesis.Q46030068
High-affinity bisubstrate probe for fluorescence anisotropy binding/displacement assays with protein kinases PKA and ROCK.Q46239703
Promiscuous fatty acyl CoA ligases produce acyl-CoA and acyl-SNAC precursors for polyketide biosynthesisQ46572050
A high-throughput screen for aggregation-based inhibition in a large compound libraryQ48080055
Nucleosides and nucleotides. 103. 2-Alkynyladenosines: a novel class of selective adenosine A2 receptor agonists with potent antihypertensive effectsQ48546271
Discovery of small-molecule inhibitors of the NFAT--calcineurin interaction by competitive high-throughput fluorescence polarization screening.Q52062082
Involvement of the fadD33 gene in the growth of Mycobacterium tuberculosis in the liver of BALB/c mice.Q52548604
A fluorescence polarization assay for screening inhibitors against the ribonuclease H activity of HIV-1 reverse transcriptase.Q53633210
A glycolipid of hypervirulent tuberculosis strains that inhibits the innate immune responseQ59057817
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequenceQ22122411
The Envelope of MycobacteriaQ22241995
Mechanistic and functional insights into fatty acid activation in Mycobacterium tuberculosisQ27653610
Genes required for mycobacterial growth defined by high density mutagenesisQ27976512
A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening AssaysQ28145712
Pathway to synthesis and processing of mycolic acids in Mycobacterium tuberculosisQ28302775
The salicylate-derived mycobactin siderophores of Mycobacterium tuberculosis are essential for growth in macrophagesQ28343891
Dissecting the role of critical residues and substrate preference of a Fatty Acyl-CoA Synthetase (FadD13) of Mycobacterium tuberculosisQ28472299
Delineation of the roles of FadD22, FadD26 and FadD29 in the biosynthesis of phthiocerol dimycocerosates and related compounds in Mycobacterium tuberculosisQ28486206
Mycobacterial phenolic glycolipid virulence factor biosynthesis: mechanism and small-molecule inhibition of polyketide chain initiationQ28486211
Dissecting the mechanism and assembly of a complex virulence mycobacterial lipidQ28486384
Persistence of Mycobacterium tuberculosis in macrophages and mice requires the glyoxylate shunt enzyme isocitrate lyaseQ28486974
Requirement of gene fadD33 for the growth of Mycobacterium tuberculosis in a hepatocyte cell lineQ28487043
The acyl-AMP ligase FadD32 and AccD4-containing acyl-CoA carboxylase are required for the synthesis of mycolic acids and essential for mycobacterial growth: identification of the carboxylation product and determination of the acyl-CoA carboxylase coQ28487115
A genetic locus required for iron acquisition in Mycobacterium tuberculosisQ28487138
Selection of transposon mutants of Mycobacterium tuberculosis with increased macrophage infectivity identifies fadD23 to be involved in sulfolipid production and association with macrophagesQ28487258
Enzymic activation and transfer of fatty acids as acyl-adenylates in mycobacteriaQ28487603
Fluorescence polarization competition assay: the range of resolvable inhibitor potency is limited by the affinity of the fluorescent ligandQ30729180
Overcoming compound interference in fluorescence polarization-based kinase assays using far-red tracersQ31067407
A specific mechanism of nonspecific inhibitionQ31166009
A general framework for development and data analysis of competitive high-throughput screens for small-molecule inhibitors of protein-protein interactions by fluorescence polarizationQ33210206
Differential expression of iron-, carbon-, and oxygen-responsive mycobacterial genes in the lungs of chronically infected mice and tuberculosis patientsQ33716960
Breaking the one antibody-one target axiomQ34715264
Development and application of fluorescence polarization assays in drug discoveryQ35100393
The dimycocerosate ester polyketide virulence factors of mycobacteria.Q36237897
Crystallization and preliminary X-ray crystallographic studies of the N-terminal domain of FadD28, a fatty-acyl AMP ligase from Mycobacterium tuberculosisQ36419717
Sulfate metabolism in mycobacteriaQ36577771
Versatile polyketide enzymatic machinery for the biosynthesis of complex mycobacterial lipids.Q36772380
P433issue2
P407language of work or nameEnglishQ1860
P921main subjectfatty acidQ61476
Mycobacterium tuberculosisQ130971
P304page(s)264-273
P577publication date2011-07-02
P1433published inAnalytical BiochemistryQ485215
P1476titleA high-throughput screening fluorescence polarization assay for fatty acid adenylating enzymes in Mycobacterium tuberculosis
P478volume417

Reverse relations

cites work (P2860)
Q33723987A fluorescence-based screen for ribosome binding antibiotics
Q37257062Adenylating enzymes in Mycobacterium tuberculosis as drug targets
Q38195645Biosynthesis of mycobacterial lipids by polyketide synthases and beyond
Q37335513Development of a one-pot assay for screening and identification of Mur pathway inhibitors in Mycobacterium tuberculosis

Search more.