Development and validation of a harmonized TaqMan-based triplex real-time RT-PCR protocol for the quantitative detection of normalized gene expression profiles of seven porcine cytokines

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Development and validation of a harmonized TaqMan-based triplex real-time RT-PCR protocol for the quantitative detection of normalized gene expression profiles of seven porcine cytokines is …
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scholarly articleQ13442814

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P819ADS bibcode2014PLoSO...9j8910P
P356DOI10.1371/JOURNAL.PONE.0108910
P932PMC publication ID4182501
P698PubMed publication ID25268123
P5875ResearchGate publication ID266324989

P50authorMartin BeerQ66386593
Sandra BlomeQ88428293
P2093author name stringAnja Petrov
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Development and evaluation of a TaqMan duplex real-time PCR quantification method for reliable enumeration of Candidatus MicrothrixQ43310197
Proinflammatory cytokine and chemokine modulation by Streptococcus suis in a whole-blood culture systemQ43509432
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Plasmids expressing porcine interferon gamma up-regulate pro-inflammatory cytokine and co-stimulatory molecule expression which are suppressed by porcine reproductive and respiratory syndrome virusQ45352118
Lymphocyte activation as cytokine gene expression and secretion is related to the porcine reproductive and respiratory syndrome virus (PRRSV) isolate after in vitro homologous and heterologous recall of peripheral blood mononuclear cells (PBMC) fromQ45353543
Healthy individuals that control a latent infection with Mycobacterium tuberculosis express high levels of Th1 cytokines and the IL-4 antagonist IL-4delta2.Q46470708
Th1 and Th2 cytokine gene expression in primary infection and vaccination against Fasciola gigantica in buffaloes by real-time PCR.Q46546715
Quantitative simultaneous multiplex real-time PCR for the detection of porcine cytokinesQ46751541
Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridizationQ46960543
Quantitation of low abundance mRNAs in glial cells using different polymerase chain reaction (PCR)-based methodsQ48639887
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P275copyright licenseCreative Commons Attribution 4.0 InternationalQ20007257
P6216copyright statuscopyrightedQ50423863
P433issue9
P407language of work or nameEnglishQ1860
P304page(s)e108910
P577publication date2014-09-30
P1433published inPLOS OneQ564954
P1476titleDevelopment and validation of a harmonized TaqMan-based triplex real-time RT-PCR protocol for the quantitative detection of normalized gene expression profiles of seven porcine cytokines
P478volume9

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