Construction and complementation of in-frame deletions of the essential Escherichia coli thymidylate kinase gene

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Construction and complementation of in-frame deletions of the essential Escherichia coli thymidylate kinase gene is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1128/AEM.72.2.1288-1294.2006
P932PMC publication ID1392977
P698PubMed publication ID16461678

P2093author name stringDavid-Nicolas Chaperon
P2860cites workExperimental Determination and System Level Analysis of Essential Genes in Escherichia coli MG1655Q22065459
Human dTMP kinase: gene expression and enzymatic activity coinciding with cell cycle progression and cell growthQ24313361
Molecular cloning and expression of the human deoxythymidylate kinase gene in yeastQ24633093
Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterizationQ24676777
Characterization of Saccharomyces cerevisiae thymidylate kinase, the CDC8 gene product. General properties, kinetic analysis, and subcellular localizationQ27936547
Human thymidylate kinase. Purification, characterization, and kinetic behavior of the thymidylate kinase derived from chronic myelocytic leukemiaQ28117079
Phosphorylation of nucleosides and nucleoside analogs by mammalian nucleoside monophosphate kinasesQ28139775
Purification and Characterization of Thymidine 5'-Monophosphate Kinase from Escherichia coli BQ28247334
Yeast gene CDC8 encodes thymidylate kinase and is complemented by herpes thymidine kinase gene TKQ28909272
Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmidQ29614535
An efficient recombination system for chromosome engineering in Escherichia coliQ29615038
Construction of versatile low-copy-number vectors for cloning, sequencing and gene expression in Escherichia coliQ29615309
The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sitesQ29618224
Role of antimetabolites of purine and pyrimidine nucleotide metabolism in tumor cell differentiationQ33691162
Substitution of an alanine residue for glycine 146 in TMP kinase from Escherichia coli is responsible for bacterial hypersensitivity to bromodeoxyuridineQ33736041
Effect of antimetabolite drugs of nucleotide metabolism on the anti-human immunodeficiency virus activity of nucleoside reverse transcriptase inhibitorsQ34047338
In vivo analysis of the overlapping functions of DnaK and trigger factorQ34165784
Bacteriophage T4 deoxynucleotide kinase: gene cloning and enzyme purificationQ36164774
Genetic mapping in Escherichia coli of tmk, the locus for dTMP kinaseQ36263076
Isolation and characterization of an Escherichia coli mutant deficient in dTMP kinase activityQ36297339
Escherichia coli thymidylate kinase: molecular cloning, nucleotide sequence, and genetic organization of the corresponding tmk locusQ36799133
Bacterial mutants in which the gene N function of bacteriophage lambda is blocked have an altered RNA polymeraseQ37493421
Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction.Q38331027
Intracellular organization of DNA precursor biosynthetic enzymesQ39572632
The delta and delta ' subunits of the DNA polymerase III holoenzyme are essential for initiation complex formation and processive elongation.Q43658434
Sequence organization and control of transcription in the bacteriophage T4 tRNA regionQ48374518
A new set of useful cloning and expression vectors derived from pBlueScript.Q54162320
A method for the construction of in frame substitutions in operons: deletion of the essential Escherichia coli holB gene coding for a subunit of the DNA polymerase III holoenzyme.Q54650045
A low-copy-number vector utilizing beta-galactosidase for the analysis of gene control elements.Q54770206
TatD Is a Cytoplasmic Protein with DNase ActivityQ57839651
Modifying Human Thymidylate Kinase to Potentiate Azidothymidine ActivationQ59662994
The bottleneck in AZT activationQ59663013
The CDC8 transcript is cell cycle regulated in yeast and is expressed coordinately with CDC9 and CDC21 at a point preceding histone transcriptionQ68181215
Cell-cycle-directed regulation of thymidylate synthase messenger RNA in human diploid fibroblasts stimulated to proliferateQ68979111
P433issue2
P407language of work or nameEnglishQ1860
P921main subjectEscherichia coliQ25419
P304page(s)1288-1294
P577publication date2006-02-01
P1433published inApplied and Environmental MicrobiologyQ4781593
P1476titleConstruction and complementation of in-frame deletions of the essential Escherichia coli thymidylate kinase gene
P478volume72

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cites work (P2860)
Q28538700Metabolic network analysis-based identification of antimicrobial drug targets in category A bioterrorism agents
Q41703445The thymidylate kinase genes from Mycobacterium tuberculosis and methicillin-resistant Staphylococcus aureus confer 3'-azido-3'-deoxythymidine resistance to Escherichia coli.

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