An efficient recombination system for chromosome engineering in Escherichia coli (scientific article (publication date: 23 May 2000))

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Q33953109	Epitope tagging of chromosomal genes in Salmonella
Q42058920	Escherichia coli Fpg glycosylase is nonrendundant and required for the rapid global repair of oxidized purine and pyrimidine damage in vivo
Q42085172	Escherichia coli SRP, its protein subunit Ffh, and the Ffh M domain are able to selectively limit membrane protein expression when overexpressed
Q35023944	Escherichia coli enoyl-acyl carrier protein reductase (FabI) supports efficient operation of a functional reversal of β-oxidation cycle
Q35759566	Essentiality of ribosomal and transcription antitermination proteins analyzed by systematic gene replacement in Escherichia coli
Q27481514	Establishment of a Bovine Herpesvirus 4 based vector expressing a secreted form of the Bovine Viral Diarrhoea Virus structural glycoprotein E2 for immunization purposes
Q43256194	Evidence against the physiological role of acetyl phosphate in the phosphorylation of the ArcA response regulator in Escherichia coli
Q44279225	Evidence for a monomeric intermediate in the reversible unfolding of F factor TraM.
Q36592350	Evidence for functional overlap among multiple bacterial cell division proteins: compensating for the loss of FtsK.
Q28238987	Evidence that feedback inhibition of NAD kinase controls responses to oxidative stress
Q24542676	Evidence that selected amplification of a bacterial lac frameshift allele stimulates Lac(+) reversion (adaptive mutation) with or without general hypermutability
Q41856093	Evidence that the promoter can influence assembly of antitermination complexes at downstream RNA sites
Q37253822	Examining a DNA Replication Requirement for Bacteriophage λ Red- and Rac Prophage RecET-Promoted Recombination in Escherichia coli
Q41487200	Excisable cassettes: new tools for functional analysis of Streptomyces genomes.
Q34429499	Excision of misincorporated ribonucleotides in DNA by RNase H (type 2) and FEN-1 in cell-free extracts
Q33224912	Expansion of substrate specificity of cytochrome P450 2A6 by random and site-directed mutagenesis
Q25256852	Experimental approaches to identify non-coding RNAs
Q40261325	Experimental confirmation of global murine cytomegalovirus open reading frames by transcriptional detection and partial characterization of newly described gene products
Q36740865	Exploring sRNA-mediated gene silencing mechanisms using artificial small RNAs derived from a natural RNA scaffold in Escherichia coli.
Q38503587	Expression and assembly of a functional type IV secretion system elicit extracytoplasmic and cytoplasmic stress responses in Escherichia coli
Q46105066	Expression and function of blimp1/krox, an alternatively transcribed regulatory gene of the sea urchin endomesoderm network
Q25257854	Expression and function profiling of orphan nuclear receptors using bacterial artificial chromosome (BAC) transgenesis
Q40164975	Expression of IroN, the salmochelin siderophore receptor, requires mRNA activation by RyhB small RNA homologues.
Q41376840	Expression of Shewanella frigidimarina fatty acid metabolic genes in E. coli by CRISPR/cas9-coupled lambda Red recombineering
Q44976896	Expression of ptsG encoding the major glucose transporter is regulated by ArcA in Escherichia coli
Q51246704	Expression of the F plasmid ccd toxin-antitoxin system in Escherichia coli cells under nutritional stress.
Q44640393	Expression of two Escherichia coli acetyl-CoA carboxylase subunits is autoregulated
Q34213476	Extending homologous sequence based on the single gene mutants by one-step PCR for efficient multiple gene knockouts
Q40702083	F-like type IV secretion systems encode proteins with thioredoxin folds that are putative DsbC homologues
Q34460338	FLEXGene repository: from sequenced genomes to gene repositories for high-throughput functional biology and proteomics
Q64884964	Failed Progenitor Specification Underlies the Cardiopharyngeal Phenotypes in a Zebrafish Model of 22q11.2 Deletion Syndrome.
Q41482036	Felid herpesvirus type 1 infection in cats: a natural host model for alphaherpesvirus pathogenesis
Q47725174	Fitness cost constrains the spectrum of marR mutations in ciprofloxacin-resistant Escherichia coli
Q36794783	Fitness of Escherichia coli mutants with reduced susceptibility to tigecycline.
Q35131867	Fluorescence-based monitoring of ribosome assembly landscapes
Q42071690	Fluorescent protein engineering by in vivo site-directed mutagenesis
Q33613251	Follistatin in chondrocytes: the link between TRPV4 channelopathies and skeletal malformations
Q36954399	Forced extraction of targeted components from complex macromolecular assemblies
Q41546224	Functional Constraints on Replacing an Essential Gene with Its Ancient and Modern Homologs
Q37781009	Functional analysis of human BRCA2 variants using a mouse embryonic stem cell-based assay.
Q38767171	Functional analysis of human cytomegalovirus UL/b' region using SCID-hu mouse model
Q24646865	Functional analysis of the protein machinery required for transport of lipopolysaccharide to the outer membrane of Escherichia coli
Q39678795	Functional and mutational analysis of conjugative transfer region 1 (Tra1) from the IncHI1 plasmid R27.
Q34580949	Functional and mutational analysis of conjugative transfer region 2 (Tra2) from the IncHI1 plasmid R27
Q33875807	Functional characterization of an alkaline exonuclease and single strand annealing protein from the SXT genetic element of Vibrio cholerae
Q33972886	Functional map of human cytomegalovirus AD169 defined by global mutational analysis
Q33459561	Functions of the Clostridium acetobutylicium FabF and FabZ proteins in unsaturated fatty acid biosynthesis
Q41911664	Fur regulates expression of the Salmonella pathogenicity island 1 type III secretion system through HilD.
Q33517847	Gene doctoring: a method for recombineering in laboratory and pathogenic Escherichia coli strains
Q47984237	Gene editing of a human gene in yeast artificial chromosomes using modified single-stranded DNA and dual targeting
Q37879979	Gene replacement techniques for Escherichia coli genome modification.
Q44508201	Gene replacement without selection: regulated suppression of amber mutations in Escherichia coli
Q34233318	Gene-specific random mutagenesis of Escherichia coli in vivo: isolation of temperature-sensitive mutations in the acyl carrier protein of fatty acid synthesis.
Q33197306	General method for the modification of different BAC types and the rapid generation of BAC transgenic mice
Q37219757	Generalized bacterial genome editing using mobile group II introns and Cre-lox
Q34015992	Generalized schemes for high-throughput manipulation of the Desulfovibrio vulgaris genome
Q41883375	Generating in vivo cloning vectors for parallel cloning of large gene clusters by homologous recombination.
Q33218071	Generating molecular diversity by homologous recombination in Escherichia coli
Q39435025	Generating tamoxifen-inducible Cre alleles to investigate myogenesis in mice
Q45294059	Generation and characterization of an EICP0 null mutant of equine herpesvirus 1.
Q44665643	Generation of an Escherichia coli lysA targeted deletion mutant by double cross-over recombination for potential use in a bacterial growth-based lysine assay
Q41980773	Generation of targeted deletions in the genome of Rhodothermus marinus
Q36728007	Genes Required for the Fitness of Salmonella enterica Serovar Typhimurium during Infection of Immunodeficient gp91-/- phox Mice
Q93095299	Genetic Engineering by DNA Recombineering
Q37396604	Genetic ablation of retinal pigment epithelial cells reveals the adaptive response of the epithelium and impact on photoreceptors.
Q40117236	Genetic analysis of varicella-zoster virus ORF0 to ORF4 by use of a novel luciferase bacterial artificial chromosome system
Q33217515	Genetic evidence that GTP is required for transposition of IS903 and Tn552 in Escherichia coli
Q36515805	Genetic interaction between the Escherichia coli AcpT phosphopantetheinyl transferase and the YejM inner membrane protein.
Q36136871	Genetic method to analyze essential genes of Escherichia coli
Q53396331	Genetic mouse models to investigate cell cycle regulation.
Q34233256	Genetic transplantation: Salmonella enterica serovar Typhimurium as a host to study sigma factor and anti-sigma factor interactions in genetically intractable systems
Q35937600	Genetics in zebrafish, mice, and humans to dissect congenital heart disease: insights in the role of VEGF.
Q38243092	Genome engineering and gene expression control for bacterial strain development
Q42251550	Genome engineering of Agrobacterium tumefaciens using the lambda Red recombination system
Q42050543	Genome modifications and cloning using a conjugally transferable recombineering system
Q34789412	Genome-scale analysis of escherichia coli FNR reveals complex features of transcription factor binding.
Q22122302	Genome-scale engineering for systems and synthetic biology
Q38003893	Genome-scale genetic manipulation methods for exploring bacterial molecular biology
Q39679833	Genomic and functional analyses of SXT, an integrating antibiotic resistance gene transfer element derived from Vibrio cholerae
Q34119146	Genomic identification of a novel mutation in hfq that provides multiple benefits in evolving glucose-limited populations of Escherichia coli.
Q31133518	Genomic resources for the study of sea urchin development
Q47327309	Germline-specific dgcr8 knockout in zebrafish using a BACK approach.
Q35983412	Gifsy-1 Prophage IsrK with Dual Function as Small and Messenger RNA Modulates Vital Bacterial Machineries
Q41448283	Global stress response in a prokaryotic model of DJ-1-associated Parkinsonism.
Q42017335	Global tRNA misacylation induced by anaerobiosis and antibiotic exposure broadly increases stress resistance in Escherichia coli
Q24490458	Global transcriptional start site mapping using differential RNA sequencing reveals novel antisense RNAs in Escherichia coli
Q37075557	Glucose- and glucokinase-controlled mal gene expression in Escherichia coli
Q33962799	Glycosylation steps during spiramycin biosynthesis in Streptomyces ambofaciens: involvement of three glycosyltransferases and their interplay with two auxiliary proteins
Q30773032	Group II introns as controllable gene targeting vectors for genetic manipulation of bacteria
Q42412151	Growth rate toxicity phenotypes and homeostatic supercoil control differentiate Escherichia coli from Salmonella enterica serovar Typhimurium
Q35943182	H-NS regulation of IraD and IraM antiadaptors for control of RpoS degradation
Q40087977	HDX-MS and deletion analysis of the type 4 secretion system protein TraF from the Escherichia coli F plasmid
Q37421362	HPr antagonizes the anti-σ70 activity of Rsd in Escherichia coli.
Q34447621	Hallmarks of homology recognition by RecA-like recombinases are exhibited by the unrelated Escherichia coli RecT protein
Q21562528	Halothiobacillus neapolitanus carboxysomes sequester heterologous and chimeric RubisCO species
Q51726574	Harvesting the genomic promise: recombineering sequences for phenotypes.
Q34958905	Hda-mediated inactivation of the DnaA protein and dnaA gene autoregulation act in concert to ensure homeostatic maintenance of the Escherichia coli chromosome
Q34247863	Herpesvirus BACs: past, present, and future
Q34731380	Herpesvirus genetics has come of age.
Q64067281	Hfq-dependent mRNA unfolding promotes sRNA-based inhibition of translation
Q33948878	High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides
Q36044894	High efficiency recombineering in lactic acid bacteria
Q43248231	High glycolytic flux improves pyruvate production by a metabolically engineered Escherichia coli strain
Q90369297	High-Efficiency Multi-site Genomic Editing of Pseudomonas putida through Thermoinducible ssDNA Recombineering
Q64056618	High-Throughput Proteomics Identifies Proteins With Importance to Postantibiotic Recovery in Depolarized Persister Cells
Q33743392	High-efficiency scarless genetic modification in Escherichia coli by using lambda red recombination and I-SceI cleavage
Q28202486	High-throughput engineering of the mouse genome coupled with high-resolution expression analysis
Q46703079	Higher plant plastids and cyanobacteria have folate carriers related to those of trypanosomatids.
Q57146141	Highly efficient base editing in bacteria using a Cas9-cytidine deaminase fusion
Q42957794	Homolactate fermentation by metabolically engineered Escherichia coli strains
Q34308000	Homologous Recombination-Experimental Systems, Analysis, and Significance
Q40079051	Homologous Recombineering to Generate Chromosomal Deletions in Escherichia coli
Q34789422	Horizontally acquired glycosyltransferase operons drive salmonellae lipopolysaccharide diversity
Q60182992	Human cis-acting elements regulating escape from X-chromosome inactivation function in mouse
Q34301880	Human cytomegalovirus entry into epithelial and endothelial cells depends on genes UL128 to UL150 and occurs by endocytosis and low-pH fusion
Q40977104	Human cytomegalovirus genes in the 15-kilobase region are required for viral replication in implanted human tissues in SCID mice
Q35901715	Human cytomegalovirus sequences expressed in latently infected individuals promote a latent infection in vitro
Q34212879	Human cytomegalovirus virion protein complex required for epithelial and endothelial cell tropism
Q40899325	Human cytomegalovirus: bacterial artificial chromosome (BAC) cloning and genetic manipulation
Q42225063	IL17 factors are early regulators in the gut epithelium during inflammatory response to Vibrio in the sea urchin larva.
Q36446432	Identification and analysis of recombineering functions from Gram-negative and Gram-positive bacteria and their phages
Q44933613	Identification and characterization of the missing pyrimidine reductase in the plant riboflavin biosynthesis pathway
Q47395379	Identification and functional analysis of potential prophage-derived recombinases for genome editing in Lactobacillus casei
Q34740554	Identification of Escherichia coli ZapC (YcbW) as a component of the division apparatus that binds and bundles FtsZ polymers
Q34319368	Identification of GtgE, a novel virulence factor encoded on the Gifsy-2 bacteriophage of Salmonella enterica serovar Typhimurium
Q36578006	Identification of MltG as a potential terminase for peptidoglycan polymerization in bacteria
Q34365923	Identification of a bifunctional enzyme MnmC involved in the biosynthesis of a hypermodified uridine in the wobble position of tRNA
Q39734941	Identification of a novel membrane-associated gene product that suppresses toxicity of a TrfA peptide from plasmid RK2 and its relationship to the DnaA host initiation protein
Q40867709	Identification of a phosphotransferase system of Escherichia coli required for growth on N-acetylmuramic acid
Q37859536	Identification of a ubiG-like gene involved in ubiquinone biosynthesis from Chlamydophila pneumoniae AR39.
Q36180500	Identification of alkA gene related to virulence of Shigella flexneri 2a by mutational analysis
Q30757004	Identification of an Archaeal type II isopentenyl diphosphate isomerase in methanothermobacter thermautotrophicus
Q39871116	Identification of an intercistronic internal ribosome entry site in a Marek's disease virus immediate-early gene
Q36337696	Identification of bacterial sRNA regulatory targets using ribosome profiling.
Q37370959	Identification of factors influencing strand bias in oligonucleotide-mediated recombination in Escherichia coli
Q35059570	Identification of new developmentally regulated genes involved in Streptomyces coelicolor sporulation.
Q36462239	Identification of protein complexes in Escherichia coli using sequential peptide affinity purification in combination with tandem mass spectrometry
Q37663494	Identification of the Escherichia coli K-12 ybhE gene as pgl, encoding 6-phosphogluconolactonase
Q28492667	Identification of two inner-membrane proteins required for the transport of lipopolysaccharide to the outer membrane of Escherichia coli
Q64063918	Identifying Small Proteins by Ribosome Profiling with Stalled Initiation Complexes
Q92188583	Impaired Efflux of the Siderophore Enterobactin Induces Envelope Stress in Escherichia coli
Q44114736	Implementation of stable and complex biological systems through recombinase-assisted genome engineering
Q41870719	Import of the transfer RNase colicin D requires site-specific interaction with the energy-transducing protein TonB.
Q34596694	Importance of the 5 S rRNA-binding ribosomal proteins for cell viability and translation in Escherichia coli
Q33588096	Improved and simplified recombineering approach for influenza virus reverse genetics.
Q36518299	Improved plasmid-based system for fully regulated off-to-on gene expression in Escherichia coli: application to production of toxic proteins
Q38257003	Improved seamless mutagenesis by recombineering using ccdB for counterselection
Q34408253	Improving lambda red genome engineering in Escherichia coli via rational removal of endogenous nucleases
Q35759792	In vivo modulation of a DnaJ homolog, CbpA, by CbpM.
Q50803970	In vivo recombineering of bacteriophage lambda by PCR fragments and single-strand oligonucleotides.
Q38386325	Inactivation of Cell Division Protein FtsZ by SulA Makes Lon Indispensable for the Viability of a ppGpp0 Strain of Escherichia coli
Q47437958	Increased expression of Qnr is sufficient to confer clinical resistance to ciprofloxacin in Escherichia coli
Q53156813	Increased expression of genes involved in uptake and degradation of murein tripeptide under nitrogen starvation in Escherichia coli.
Q33293398	Inducible gene inactivation in neurons of the adult mouse forebrain
Q36074341	Inefficient replication reduces RecA-mediated repair of UV-damaged plasmids introduced into competent Escherichia coli
Q50283166	Inhibition of RecBCD in Klebsiella pneumoniae by Gam and its effect on the efficiency of gene replacement
Q28259871	Insertional mutagenesis in mice: new perspectives and tools
Q21090607	Insights into the molecular evolution of HslU ATPase through biochemical and mutational analyses
Q39694438	Integrating global regulatory input into the Salmonella pathogenicity island 1 type III secretion system
Q33726379	Intein-mediated cyclization of bacterial acyl carrier protein stabilizes its folded conformation but does not abolish function
Q35759654	Interaction between cell division proteins FtsE and FtsZ.
Q29615776	Interaction network containing conserved and essential protein complexes in Escherichia coli
Q83230765	Interactions between a subset of substrate side chains and AAA+ motor pore loops determine grip during protein unfolding
Q36172152	Interactions between proteins encoded within the human cytomegalovirus UL133-UL138 locus.
Q42699139	Interactions of the Escherichia coli hydrogenase biosynthetic proteins: HybG complex formation
Q40490800	Interchangeable domains in the Kdo transferases of Escherichia coli and Haemophilus influenzae
Q35738776	Interplay between DNA replication, recombination and repair based on the structure of RecG helicase
Q34002342	Interplay between plasmid partition and postsegregational killing systems
Q28475870	Interplay in the selection of fluoroquinolone resistance and bacterial fitness
Q28235185	Interplay of transcriptional and small RNA-dependent control mechanisms regulates chitosugar uptake in Escherichia coli and Salmonella
Q36577171	Intestinal Long-Chain Fatty Acids Act as a Direct Signal To Modulate Expression of the Salmonella Pathogenicity Island 1 Type III Secretion System
Q85178136	Introduction of large DNA inserts into the barley pathogenic fungus, Ustilago hordei, via recombined binary BAC vectors and Agrobacterium-mediated transformation
Q50656575	Involvement of DNA replication in phage lambda Red-mediated homologous recombination.
Q33249072	IscR acts as an activator in response to oxidative stress for the suf operon encoding Fe-S assembly proteins
Q42201800	Isolation of lactococcal prolate phage-phage recombinants by an enrichment strategy reveals two novel host range determinants.
Q34110739	Isolation of rare recombinants without using selectable markers for one-step seamless BAC mutagenesis.
Q33730200	Isolation of specific clones from nonarrayed BAC libraries through homologous recombination
Q24813037	Iterative in vivo assembly of large and complex transgenes by combining the activities of phiC31 integrase and Cre recombinase.
Q36996756	Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA sequences, including whole metabolic pathways, onto the Escherichia coli chromosome at well-characterised loci
Q36821495	LNA modification of single-stranded DNA oligonucleotides allows subtle gene modification in mismatch-repair-proficient cells
Q36538725	Lambda Red-mediated Recombineering in the Attaching and Effacing Pathogen Escherichia albertii
Q24804947	Lambda Red-mediated recombinogenic engineering of enterohemorrhagic and enteropathogenic E. coli
Q35585253	Lambda red mediated gap repair utilizes a novel replicative intermediate in Escherichia coli
Q34285766	Lambda red recombineering in Escherichia coli occurs through a fully single-stranded intermediate.
Q35835052	Lambda red-mediated genetic manipulation of antibiotic-producing Streptomyces.
Q38822434	Large scale validation of an efficient CRISPR/Cas-based multi gene editing protocol in Escherichia coli
Q40950520	Large-scale recoding of a bacterial genome by iterative recombineering of synthetic DNA.
Q64389179	Limited Capacity or Involvement of Excision Repair, Double-Strand Breaks, or Translesion Synthesis for Psoralen Cross-Link Repair in
Q24679480	Limited role for the DsrA and RprA regulatory RNAs in rpoS regulation in Salmonella enterica
Q30158006	Lipoprotein SmpA is a component of the YaeT complex that assembles outer membrane proteins in Escherichia coli
Q42591973	Localization of an accessory helicase at the replisome is critical in sustaining efficient genome duplication
Q34004070	Lon protease quality control of presecretory proteins in Escherichia coli and its dependence on the SecB and DnaJ (Hsp40) chaperones
Q42117522	Long-Term Persistence of Bi-functionality Contributes to the Robustness of Microbial Life through Exaptation
Q36209777	Long-Term Stable and Tightly Controlled Expression of Recombinant Proteins in Antibiotics-Free Conditions
Q34244961	Long-range chromosome organization in E. coli: a site-specific system isolates the Ter macrodomain
Q34568672	Loss of Hfq activates the sigmaE-dependent envelope stress response in Salmonella enterica
Q33216936	Loss of outer membrane proteins without inhibition of lipid export in an Escherichia coli YaeT mutant
Q81441872	MAGIC, an in vivo genetic method for the rapid construction of recombinant DNA molecules
Q63359441	Maintenance of embryonic stem cell pluripotency by Nanog-mediated reversal of mesoderm specification
Q92565699	Manipulating the Mouse Genome Using Recombineering
Q42071778	Mapping bacterial functional networks and pathways in Escherichia Coli using synthetic genetic arrays
Q36856571	Marek's disease virus research in the post-sequencing era: new tools for the study of gene functions and virus-host interactions
Q38048435	Mathematical optimization applications in metabolic networks
Q34192636	Mechanism of positive regulation by DsrA and RprA small noncoding RNAs: pairing increases translation and protects rpoS mRNA from degradation
Q54307659	Mechanisms and fitness costs of tigecycline resistance in Escherichia coli.
Q50683353	Mechanisms controlling Pax6 isoform expression in the retina have been conserved between teleosts and mammals.
Q35213357	Membrane localization of small proteins in Escherichia coli
Q39680104	Membrane protein degradation by FtsH can be initiated from either end.
Q48887186	Memory CD4 T cells emerge from effector T-cell progenitors
Q35096012	Metabolic engineering of Escherichia coli for production of enantiomerically pure (R)-(--)-hydroxycarboxylic acids
Q42528920	Metal-specific control of gene expression mediated by Bradyrhizobium japonicum Mur and Escherichia coli Fur is determined by the cellular context
Q24629569	MicA sRNA links the PhoP regulon to cell envelope stress
Q28244052	MicL, a new σE-dependent sRNA, combats envelope stress by repressing synthesis of Lpp, the major outer membrane lipoprotein
Q47128104	Micro-patterned agarose gel devices for single-cell high-throughput microscopy of E. coli cells.
Q31114581	Microbial technologies for the discovery of novel bioactive metabolites.
Q51812826	Mini-λ: a tractable system for chromosome and BAC engineering
Q47169874	Minimizing carry-over PCR contamination in expanded CAG/CTG repeat instability applications.
Q35657323	MioC and GidA proteins promote cell division in E. coli
Q33519503	Mobile antibiotic resistance encoding elements promote their own diversity
Q34661172	Modeling Neuropsychiatric Disease-Relevant Human SNPs in Mice
Q34187023	Modelling human regulatory variation in mouse: finding the function in genome-wide association studies and whole-genome sequencing
Q30842663	Modelling within-host spatiotemporal dynamics of invasive bacterial disease
Q38264064	Modern and simple construction of plasmid: saving time and cost
Q34811166	Modification of the RpoS network with a synthetic small RNA.
Q35198341	Modified bases enable high-efficiency oligonucleotide-mediated allelic replacement via mismatch repair evasion
Q34649165	Modulating RssB activity: IraP, a novel regulator of sigma(S) stability in Escherichia coli
Q33967263	Modulation of UvrD helicase activity by covalent DNA-protein cross-links
Q34232725	Molecular analysis of the O-antigen gene cluster of Escherichia coli O86:B7 and characterization of the chain length determinant gene (wzz).
Q33791248	Molecular characterization of the beta-N-acetylglucosaminidase of Escherichia coli and its role in cell wall recycling
Q34011834	Mu and IS1 transpositions exhibit strong orientation bias at the Escherichia coli bgl locus
Q28589969	Muc5b is required for airway defence
Q37252418	Multicopy plasmid modification with phage lambda Red recombineering
Q35172182	Multigene editing in the Escherichia coli genome via the CRISPR-Cas9 system
Q37252997	Multiple Transcripts Encode Full-Length Human Cytomegalovirus IE1 and IE2 Proteins during Lytic Infection
Q30828946	Multiple upstream modules regulate zebrafish myf5 expression
Q31127364	Multiple, distant Gata2 enhancers specify temporally and tissue-specific patterning in the developing urogenital system
Q50443068	Multiple-site genetic modifications in Escherichia coli using lambda-Red recombination and I-SceI cleavage.
Q64388766	Multiple-step chromosomal integration of divided segments from a large DNA fragment via CRISPR/Cas9 in Escherichia coli
Q47318371	Multiplex Genome Editing in Escherichia coli
Q97636434	Multiplexed genomic encoding of non-canonical amino acids for labeling large complexes
Q60954930	Muramyl Endopeptidase Spr Contributes to Intrinsic Vancomycin Resistance in Serovar Typhimurium
Q35722318	Murine cytomegalovirus encodes a stable intron that facilitates persistent replication in the mouse
Q40005609	Murine cytomegalovirus m38.5 protein inhibits Bax-mediated cell death.
Q37165587	Mutagenesis of the murine cytomegalovirus M56 terminase gene
Q64260692	Mutagenesis: A Method for Large-Scale Alteration of Viral Genomes
Q36590556	Mutation accumulation and fitness in mutator subpopulations of Escherichia coli
Q37372690	Mutational analysis of Escherichia coli sigma28 and its target promoters reveals recognition of a composite -10 region, comprised of an 'extended -10' motif and a core -10 element.
Q36046517	Mutations Affecting Potassium Import Restore the Viability of the Escherichia coli DNA Polymerase III holD Mutant
Q46889740	Mutations that alter the regulation of the chb operon of Escherichia coli allow utilization of cellobiose
Q34692112	Mutualistic interaction between Salmonella enterica and Aspergillus niger and its effects on Zea mays colonization
Q33686972	NANOG reporter cell lines generated by gene targeting in human embryonic stem cells.
Q36684736	Nanobody(R)-based chromatin immunoprecipitation/micro-array analysis for genome-wide identification of transcription factor DNA binding sites
Q28585886	Nanog binds to Smad1 and blocks bone morphogenetic protein-induced differentiation of embryonic stem cells
Q28730500	Natural DNA uptake by Escherichia coli
Q47379825	Neurogenin 3-expressing progenitor cells in the gastrointestinal tract differentiate into both endocrine and non-endocrine cell types
Q77689720	New approaches and old problems in the shadow of bioterrorism
Q36646760	Nicotinic acid modulates Legionella pneumophila gene expression and induces virulence traits
Q42228666	NinR- and red-mediated phage-prophage marker rescue recombination in Escherichia coli: recovery of a nonhomologous immlambda DNA segment by infecting lambdaimm434 phages
Q89820537	Nitrate Metabolism Modulates Biosynthesis of Biofilm Components in Uropathogenic Escherichia coli and Acts as a Fitness Factor During Experimental Urinary Tract Infection
Q41911689	NlpD links cell wall remodeling and outer membrane invagination during cytokinesis in Escherichia coli
Q36097374	Nodal signals mediate interactions between the extra-embryonic and embryonic tissues in zebrafish
Q33588592	Non-coding-regulatory regions of human brain genes delineated by bacterial artificial chromosome knock-in mice
Q42174256	Notch signaling differentially regulates the cell fate of early endocrine precursor cells and their maturing descendants in the mouse pancreas and intestine
Q50067095	Novel Metabolic Pathway for N-Methylpyrrolidone Degradation in Alicycliphilus sp. Strain BQ1.
Q96229602	Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling
Q36532663	Nus transcription elongation factors and RNase III modulate small ribosome subunit biogenesis in Escherichia coli.
Q60303471	ORBIT: a New Paradigm for Genetic Engineering of Mycobacterial Chromosomes
Q36114524	Oligonucleotide recombination in Gram-negative bacteria
Q42579568	Oligonucleotide recombination: a hidden treasure
Q42052427	Oligoribonuclease is a common downstream target of lithium-induced pAp accumulation in Escherichia coli and human cells
Q42556414	One-carbon metabolic pathway rewiring in Escherichia coli reveals an evolutionary advantage of 10-formyltetrahydrofolate synthetase (Fhs) in survival under hypoxia
Q92314731	Operon Concatenation Is an Ancient Feature That Restricts the Potential to Rearrange Bacterial Chromosomes
Q35987332	Oscillating focus of SopA associated with filamentous structure guides partitioning of F plasmid.
Q80155095	Overexpression of pairedless Pax6 in the retina disrupts corneal development and affects lens cell survival
Q42121314	Overproduction of exopolysaccharides by an Escherichia coli K-12 rpoS mutant in response to osmotic stress
Q37939709	Overview of antibacterial target selection
Q33789736	Oxidation of cellular amino acid pools leads to cytotoxic mistranslation of the genetic code
Q47296673	OxyS small RNA induces cell cycle arrest to allow DNA damage repair
Q93366073	Oxygen-dependent regulation of SPI1 type three secretion system by small RNAs in Salmonella enterica serovar Typhimurium
Q30764407	PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin
Q53288337	Partitioning of P1 plasmids by gradual distribution of the ATPase ParA.
Q34325596	Patterns of evolutionary conservation of essential genes correlate with their compensability
Q50505034	Peptide vaccination is superior to genetic vaccination using a recombineered bacteriophage λ subunit vaccine.
Q36893539	Periplasmic orientation of nascent lipid A in the inner membrane of an Escherichia coli LptA mutant
Q34982806	Phage HK022 Nun protein represses translation of phage lambda N (transcription termination/translation repression).
Q59360291	Phage Therapy: What Have We Learned?
Q24798568	Phage annealing proteins promote oligonucleotide-directed mutagenesis in Escherichia coli and mouse ES cells
Q39741226	Phenotypes of lexA mutations in Salmonella enterica: evidence for a lethal lexA null phenotype due to the Fels-2 prophage
Q33219967	Phenotypic alteration and target gene identification using combinatorial libraries of zinc finger proteins in prokaryotic cells
Q92641059	PhoP-Mediated Repression of the SPI1 Type 3 Secretion System in Salmonella enterica Serovar Typhimurium
Q33675769	Phosphoglucomutase of Yersinia pestis is required for autoaggregation and polymyxin B resistance.
Q36978654	Phosphorylation of Kaposi's sarcoma-associated herpesvirus processivity factor ORF59 by a viral kinase modulates its ability to associate with RTA and oriLyt
Q54425023	Phosphorylation of the integrase protein of coliphage HK022.
Q34279829	Phosphotransferase-mediated transport of the osmolyte 2-O-alpha-mannosyl-D-glycerate in Escherichia coli occurs by the product of the mngA (hrsA) gene and is regulated by the mngR (farR) gene product acting as repressor
Q24605734	Physiological consequences of multiple-target regulation by the small RNA SgrS in Escherichia coli
Q33757023	Point mutation of bacterial artificial chromosomes by ET recombination
Q54345642	Polymerase exchange during Okazaki fragment synthesis observed in living cells.
Q38342098	Positive and negative determinants of target gene specificity in myb transcription factors.
Q34383416	Positive and negative selection using the tetA-sacB cassette: recombineering and P1 transduction in Escherichia coli
Q34642010	Post-transcriptional regulation of the E/Daughterless ortholog HLH-2, negative feedback, and birth order bias during the AC/VU decision in C. elegans
Q33560129	Potential link between the NIMA mitotic kinase and nuclear membrane fission during mitotic exit in Aspergillus nidulans
Q35856281	Preaxial polydactyly caused by Gli3 haploinsufficiency is rescued by Zic3 loss of function in mice
Q28243372	Precise manipulation of chromosomes in vivo enables genome-wide codon replacement
Q38245846	Precision genome engineering in lactic acid bacteria
Q34612552	Probing cellular processes with oligo-mediated recombination and using the knowledge gained to optimize recombineering
Q34378484	Probing the limits of genetic recoding in essential genes.
Q47245119	Probing young drinking water biofilms with hard and soft particles
Q28661058	Production of medium chain length fatty alcohols from glucose in Escherichia coli
Q36434513	Production of recombineering substrates with standard-size PCR primers
Q28253066	Programming cells by multiplex genome engineering and accelerated evolution
Q50727134	Progressive segregation of the Escherichia coli chromosome.
Q35554051	Prominent roles of the NorR and Fur regulators in the Escherichia coli transcriptional response to reactive nitrogen species
Q33406825	Prophage induction is enhanced and required for renal disease and lethality in an EHEC mouse model
Q36127135	Prophage recombinases-mediated genome engineering in Lactobacillus plantarum
Q24658383	Proteolytic inactivation of tissue factor pathway inhibitor by bacterial omptins
Q24813315	Protocol: precision engineering of plant gene loci by homologous recombination cloning in Escherichia coli
Q44765735	Proximal and distal sequences control UV cone pigment gene expression in transgenic zebrafish
Q33798792	Pyruvate-associated acid resistance in bacteria
Q29039105	Quantifying E. coli Proteome and Transcriptome with Single-Molecule Sensitivity in Single Cells
Q33908536	RMDAP: a versatile, ready-to-use toolbox for multigene genetic transformation
Q40554110	RNA remodeling by bacterial global regulator CsrA promotes Rho-dependent transcription termination
Q37849324	RNase HIII from Chlamydophila pneumoniae can efficiently cleave double-stranded DNA carrying a chimeric ribonucleotide in the presence of manganese
Q34538123	RNase III participates in GadY-dependent cleavage of the gadX-gadW mRNA.
Q33270006	RNaseE and the other constituents of the RNA degradosome are components of the bacterial cytoskeleton
Q50673090	RT-qPCR with chimeric dU stem-loop primer is efficient for the detection of bacterial small RNAs.
Q41727693	Rapid and efficient construction of markerless deletions in the Escherichia coli genome
Q35238418	Rapid editing and evolution of bacterial genomes using libraries of synthetic DNA.
Q40130517	Rapid engineering of bacterial reporter gene fusions by using Red recombination.
Q34553401	Rapid genetic engineering of human cytomegalovirus by using a lambda phage linear recombination system: demonstration that pp28 (UL99) is essential for production of infectious virus.
Q41868385	Rapid oligonucleotide-based recombineering of the chromosome of Salmonella enterica.
Q33635299	Rapid profiling of a microbial genome using mixtures of barcoded oligonucleotides
Q37537895	Rapid tagging of endogenous mouse genes by recombineering and ES cell complementation of tetraploid blastocysts
Q34390566	Rates of gyrase supercoiling and transcription elongation control supercoil density in a bacterial chromosome
Q51171188	Rational design and metabolic analysis of Escherichia coli for effective production of L-tryptophan at high concentration.
Q37173130	Rational elicitation of cold-sensitive phenotypes.
Q37701249	Rational optimization of tolC as a powerful dual selectable marker for genome engineering
Q64387515	RecBCD, SbcCD and ExoI process a substrate created by convergent replisomes to complete DNA replication
Q24603059	Recognition and discrimination of target mRNAs by Sib RNAs, a cis-encoded sRNA family
Q41517882	Recognition of the 70S ribosome and polysome by the RNA degradosome in Escherichia coli.
Q33858878	Recombination phenotypes of Escherichia coli greA mutants
Q34999929	Recombination promoted by DNA viruses: phage λ to herpes simplex virus.
Q34317190	Recombination-promoting activity of the bacteriophage lambda Rap protein in Escherichia coli K-12
Q37792222	Recombineering BAC transgenes for protein tagging
Q34695274	Recombineering in Corynebacterium glutamicum combined with optical nanosensors: a general strategy for fast producer strain generation
Q54449978	Recombineering in Mycobacterium tuberculosis.
Q36414625	Recombineering mycobacteria and their phages
Q38084259	Recombineering to homogeneity: extension of multiplex recombineering to large-scale genome editing
Q34045936	Recombineering using RecTE from Pseudomonas syringae
Q35163354	Recombineering with overlapping single-stranded DNA oligonucleotides: testing a recombination intermediate
Q43053415	Recombineering with tolC as a selectable/counter-selectable marker: remodeling the rRNA operons of Escherichia coli
Q51551994	Recombineering, transfection, Western, IP and ChIP methods for protein tagging via gene targeting or BAC transgenesis.
Q34293061	Recombineering: A powerful tool for modification of bacteriophage genomes
Q28307909	Recombineering: a homologous recombination-based method of genetic engineering
Q34389493	Recombineering: a powerful new tool for mouse functional genomics
Q64390429	Recombineering: highly efficient in vivo genetic engineering using single-strand oligos
Q36757817	Recombineering: in vivo genetic engineering in E. coli, S. enterica, and beyond
Q38311484	Recombineering: using drug cassettes to knock out genes in vivo
Q24805845	Recombining overlapping BACs into a single larger BAC
Q39527528	Reconstruction of Escherichia coli mrcA (PBP 1a) mutants lacking multiple combinations of penicillin binding proteins
Q24522689	Recql5 and Blm RecQ DNA helicases have nonredundant roles in suppressing crossovers
Q43083679	Redefining the essential trafficking pathway for outer membrane lipoproteins
Q28485150	Redirector: designing cell factories by reconstructing the metabolic objective
Q46414003	Redistribution of metabolic fluxes in the central aerobic metabolic pathway of E. coli mutant strains with deletion of the ackA-pta and poxB pathways for the synthesis of isoamyl acetate
Q35109087	Reducing ppGpp level rescues an extreme growth defect caused by mutant EF-Tu
Q39657850	Regulated expression of the Escherichia coli lepB gene as a tool for cellular testing of antimicrobial compounds that inhibit signal peptidase I in vitro
Q34485023	Regulation and Function of Escherichia coli Sugar Efflux Transporter A (SetA) during Glucose-Phosphate Stress
Q40605925	Regulation of 6S RNA biogenesis by switching utilization of both sigma factors and endoribonucleases
Q36280073	Regulation of cell size in response to nutrient availability by fatty acid biosynthesis in Escherichia coli
Q41869989	Regulation of iron-sulphur cluster homeostasis through transcriptional control of the Isc pathway by [2Fe-2S]-IscR in Escherichia coli
Q34434306	Regulation of mutY and nature of mutator mutations in Escherichia coli populations under nutrient limitation
Q34192714	Regulation of the Biosynthesis of the Macrolide Antibiotic Spiramycin in Streptomyces ambofaciens
Q33758224	Regulation of virulence by a two-component system in group B streptococcus
Q30493411	Regulatory activity revealed by dynamic correlations in gene expression noise
Q37132764	Remodeling bacterial polysaccharides by metabolic pathway engineering
Q28287313	Remodelling of the Escherichia coli outer membrane by two small regulatory RNAs
Q41943926	Removal of deoxyinosine from the Escherichia coli chromosome as studied by oligonucleotide transformation
Q35144337	Repetitive genomic insertion of gene-sized dsDNAs by targeting the promoter region of a counter-selectable marker
Q89698155	Replication Rapidly Recovers and Continues in the Presence of Hydroxyurea in Escherichia coli
Q39116572	Replication fork blockage by transcription factor-DNA complexes in Escherichia coli.
Q24648490	Repression of small toxic protein synthesis by the Sib and OhsC small RNAs
Q24606364	Reprogramming of anaerobic metabolism by the FnrS small RNA
Q34312339	Requirement for NusG for transcription antitermination in vivo by the lambda N protein
Q28506307	Requirement of regulated endocrine-specific protein-18 for development and expression of regulated endocrine-specific protein-18 isoform c in mice
Q46734988	Requirements for ATP binding and hydrolysis in RecA function in Escherichia coli
Q31011523	Resistance of human cytomegalovirus to the benzimidazole L-ribonucleoside maribavir maps to UL27
Q40623370	Restoration of gene function by homologous recombination: from PCR to gene expression in one step.
Q35826670	Retina restored and brain abnormalities ameliorated by single-copy knock-in of human NR2E1 in null mice
Q34452446	Rho-dependent termination of ssrS (6S RNA) transcription in Escherichia coli: implication for 3' processing of 6S RNA and expression of downstream ygfA (putative 5-formyl-tetrahydrofolate cyclo-ligase).
Q42462291	Ribosome Assembly as Antimicrobial Target
Q41865056	Robust translation of the nucleoid protein Fis requires a remote upstream AU element and is enhanced by RNA secondary structure
Q42957499	Role of FtsEX in cell division of Escherichia coli: viability of ftsEX mutants is dependent on functional SufI or high osmotic strength
Q34077220	Role of RcsF in signaling to the Rcs phosphorelay pathway in Escherichia coli
Q42910178	Role of SufI (FtsP) in cell division of Escherichia coli: evidence for its involvement in stabilizing the assembly of the divisome
Q36944070	Role of a mutation in human cytomegalovirus gene UL104 in resistance to benzimidazole ribonucleosides
Q35096168	Role of maltose enzymes in glycogen synthesis by Escherichia coli
Q34985832	Role of polynucleotide phosphorylase in sRNA function in Escherichia coli
Q39680255	Role of ppGpp in rpoS stationary-phase regulation in Escherichia coli
Q37596556	Role of the proximal enhancer of the major immediate-early promoter in human cytomegalovirus replication
Q33892349	Roles of steroid receptor coactivator (SRC)-1 and transcriptional intermediary factor (TIF) 2 in androgen receptor activity in mice.
Q34489721	RppH-dependent pyrophosphohydrolysis of mRNAs is regulated by direct interaction with DapF in Escherichia coli
Q35662912	RtsA and RtsB coordinately regulate expression of the invasion and flagellar genes in Salmonella enterica serovar Typhimurium.
Q34555106	RtsA coordinately regulates DsbA and the Salmonella pathogenicity island 1 type III secretion system
Q35906084	SLiCE: a novel bacterial cell extract-based DNA cloning method
Q35123249	SUMOylation of the human cytomegalovirus 72-kilodalton IE1 protein facilitates expression of the 86-kilodalton IE2 protein and promotes viral replication
Q28766733	Salmonella enterica serovar Typhimurium periplasmic superoxide dismutase SodCI is a member of the PhoPQ regulon and is induced in macrophages
Q33648542	Scarless and sequential gene modification in Pseudomonas using PCR product flanked by short homology regions
Q34429418	Scission of the lactyl ether bond of N-acetylmuramic acid by Escherichia coli "etherase".
Q40567039	Screening for synthetic lethal mutants in Escherichia coli and identification of EnvC (YibP) as a periplasmic septal ring factor with murein hydrolase activity.
Q28775689	Secretion of LamB-LacZ by the signal recognition particle pathway of Escherichia coli
Q52552501	Segregation of the Escherichia coli chromosome terminus
Q42688968	Self-enhanced accumulation of FtsN at Division Sites and Roles for Other Proteins with a SPOR domain (DamX, DedD, and RlpA) in Escherichia coli cell constriction
Q34531680	Sequencing genomes from single cells by polymerase cloning
Q42644391	Seven gene deletions in seven days: Fast generation of Escherichia coli strains tolerant to acetate and osmotic stress
Q24794075	Simple and highly efficient BAC recombineering using galK selection
Q38268186	Simple generation of site-directed point mutations in the Escherichia coli chromosome using Red(R)/ET(R) Recombination
Q30501142	Single-cell time-lapse analysis of depletion of the universally conserved essential protein YgjD
Q60359855	Single-nucleotide-resolution mapping of DNA gyrase cleavage sites across the Escherichia coli genome.
Q33644920	Single-stranded heteroduplex intermediates in lambda Red homologous recombination
Q33760274	Site-specific chromosomal integration of large synthetic constructs
Q54528961	Site-specific gene targeting in mouse embryonic stem cells with intact bacterial artificial chromosomes.
Q34220676	Size matters: use of YACs, BACs and PACs in transgenic animals
Q36342728	Small RNA binding-site multiplicity involved in translational regulation of a polycistronic mRNA.
Q24654108	Small RNAs and small proteins involved in resistance to cell envelope stress and acid shock in Escherichia coli: analysis of a bar-coded mutant collection
Q37039330	Small membrane proteins found by comparative genomics and ribosome binding site models.
Q24651398	Small noncoding RNA GcvB is a novel regulator of acid resistance in Escherichia coli
Q33297912	SmeA, a small membrane protein with multiple functions in Streptomyces sporulation including targeting of a SpoIIIE/FtsK-like protein to cell division septa
Q33937781	Species and incompatibility determination within the P1par family of plasmid partition elements
Q35145464	Sphingosine-1-phosphate lyase downregulation promotes colon carcinogenesis through STAT3-activated microRNAs
Q53861487	SspA is required for acid resistance in stationary phase by downregulation of H-NS in Escherichia coli.
Q39361630	Stationary-phase regulation of RpoS translation in Escherichia coli
Q83864142	Strain engineering by genome mass transfer: efficient chromosomal trait transfer method utilizing donor genomic DNA and recipient recombineering hosts
Q38321952	Strategies for cloning and manipulating natural and synthetic chromosomes
Q28066046	Strategies used for genetically modifying bacterial genome: site-directed mutagenesis, gene inactivation, and gene over-expression
Q34120107	Stress-inducible alternative translation initiation of human cytomegalovirus latency protein pUL138.
Q33716635	Structural and genetic characterization of the Shigella boydii type 10 and type 6 O antigens
Q24598818	Structural and genetic characterization of the Shigella boydii type 13 O antigen
Q96641424	Structural insight into the formation of lipoprotein-β-barrel complexes
Q33264704	Structure and biosynthesis of free lipid A molecules that replace lipopolysaccharide in Francisella tularensis subsp. novicida
Q89664204	Structure of an Inner Membrane Protein Required for PhoPQ-Regulated Increases in Outer Membrane Cardiolipin
Q59137850	Structure-based mechanism of action of a viral poly(ADP-ribose) polymerase 1-interacting protein facilitating virus replication
Q34363302	Structure-function analysis of MurJ reveals a solvent-exposed cavity containing residues essential for peptidoglycan biogenesis in Escherichia coli
Q50026573	Structure-function analysis of manganese exporter proteins across bacteria
Q51684653	Studies on the production of shikimic acid using the aroK knockout strain of Bacillus megaterium.
Q41437261	Studying the properties of domain I of the ribosomal protein l1: incorporation into ribosome and regulation of the l1 operon expression
Q35161843	Subcloning plus insertion (SPI)--a novel recombineering method for the rapid construction of gene targeting vectors
Q34505633	Substrate and target sequence length influence RecTE(Psy) recombineering efficiency in Pseudomonas syringae
Q36727639	SuhB Associates with Nus Factors To Facilitate 30S Ribosome Biogenesis in Escherichia coli
Q33726839	Surprising dependence on postsegregational killing of host cells for maintenance of the large virulence plasmid of Shigella flexneri
Q41448496	Survival and Evolution of a Large Multidrug Resistance Plasmid in New Clinical Bacterial Hosts.
Q21558764	Survival of the fittest tools
Q31036586	Sustained CD8+ T cell memory inflation after infection with a single-cycle cytomegalovirus
Q36973350	Synchronization of endothelial Dll4-Notch dynamics switch blood vessels from branching to expansion
Q90639322	Synonymous Mutations in rpsT Lead to Ribosomal Assembly Defects That Can Be Compensated by Mutations in fis and rpoA
Q35850231	Synthesis of glycerol phosphate lipoteichoic acid in Staphylococcus aureus
Q35271224	Synthesis of the heteropolysaccharide O antigen of Escherichia coli O52 requires an ABC transporter: structural and genetic evidence
Q55375263	Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria.
Q27001264	Synthetic biology of antimicrobial discovery
Q34702375	Synthetic biology. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations
Q37974010	Synthetic biology: new strategies for directing design
Q36923772	Synthetic growth phenotypes of Escherichia coli lacking ppGpp and transketolase A (tktA) are due to ppGpp-mediated transcriptional regulation of tktB.
Q33665680	Syntrophic exchange in synthetic microbial communities
Q49499486	Systematic Dissection of Sequence Elements Controlling σ70 Promoters Using a Genomically-Encoded Multiplexed Reporter Assay in E. coli
Q47213847	Systematic characterization of maturation time of fluorescent proteins in living cells.
Q33980052	Systematic mutagenesis of the Escherichia coli genome
Q41641077	T-cell development is regulated by the coordinated function of proximal and distal Lck promoters active at different developmental stages
Q30479063	TINA interacts with the NIMA kinase in Aspergillus nidulans and negatively regulates astral microtubules during metaphase arrest
Q24544245	Target prediction for small, noncoding RNAs in bacteria
Q92059508	Targeted and Repetitive Chromosomal Integration Enables High-Level Heterologous Gene Expression in Lactobacillus casei
Q35887169	Targeted deletion of thioesterase superfamily member 1 promotes energy expenditure and protects against obesity and insulin resistance
Q47417619	Targeted mutagenesis: A sniper-like diversity generator in microbial engineering
Q34617095	Targeted nucleotide repair of cyc1 mutations in Saccharomyces cerevisiae directed by modified single-stranded DNA oligonucleotides
Q27972919	Targeting NAD+ metabolism in the human malaria parasite Plasmodium falciparum
Q40262965	Targeting iCre expression to murine progesterone receptor cell-lineages using bacterial artificial chromosome transgenesis
Q37087036	Technical considerations in using DNA microarrays to define regulons.
Q54975264	Technique of laser chromosome welding for chromosome repair and artificial chromosome creation.
Q34245450	Techniques: Recombinogenic engineering--new options for cloning and manipulating DNA.
Q36747396	Temperature sensitivity and cell division defects in an Escherichia coli strain with mutations in yghB and yqjA, encoding related and conserved inner membrane proteins
Q36517922	The CBS subdomain of inosine 5'-monophosphate dehydrogenase regulates purine nucleotide turnover
Q33703476	The Carboxysome Shell Is Permeable to Protons
Q34428433	The CnuK9E H-NS complex antagonizes DNA binding of DicA and leads to temperature-dependent filamentous growth in E. coli
Q40475276	The Cpx envelope stress response affects expression of the type IV bundle-forming pili of enteropathogenic Escherichia coli.
Q27668177	The Era GTPase recognizes the GAUCACCUCC sequence and binds helix 45 near the 3' end of 16S rRNA
Q34215344	The Escherichia coli MntR miniregulon includes genes encoding a small protein and an efflux pump required for manganese homeostasis
Q40565200	The Escherichia coli amidase AmiC is a periplasmic septal ring component exported via the twin-arginine transport pathway
Q34305324	The Escherichia coli cell division protein FtsW is required to recruit its cognate transpeptidase, FtsI (PBP3), to the division site.
Q34862873	The FLEXGene repository: exploiting the fruits of the genome projects by creating a needed resource to face the challenges of the post-genomic era.
Q28593412	The HSV-1 exonuclease, UL12, stimulates recombination by a single strand annealing mechanism
Q35126034	The Kil peptide of bacteriophage λ blocks Escherichia coli cytokinesis via ZipA-dependent inhibition of FtsZ assembly
Q42733384	The Little Fly that Could: Wizardry and Artistry of Drosophila Genomics.
Q36132532	The MaoP/maoS Site-Specific System Organizes the Ori Region of the E. coli Chromosome into a Macrodomain.
Q41874281	The MoxR ATPase RavA and its cofactor ViaA interact with the NADH:ubiquinone oxidoreductase I in Escherichia coli
Q36106787	The O-Antigen Flippase Wzk Can Substitute for MurJ in Peptidoglycan Synthesis in Helicobacter pylori and Escherichia coli
Q39679036	The O-antigen gene cluster of Escherichia coli O55:H7 and identification of a new UDP-GlcNAc C4 epimerase gene
Q34910861	The OmpL porin does not modulate redox potential in the periplasmic space of Escherichia coli
Q37635066	The PAX258 gene subfamily: a comparative perspective
Q37425414	The Phosphorolytic Exoribonucleases Polynucleotide Phosphorylase and RNase PH Stabilize sRNAs and Facilitate Regulation of Their mRNA Targets
Q46210806	The Promiscuous sumA Missense Suppressor from Salmonella enterica Has an Intriguing Mechanism of Action
Q33880011	The SOS and RpoS Regulons Contribute to Bacterial Cell Robustness to Genotoxic Stress by Synergistically Regulating DNA Polymerase Pol II.
Q28492915	The Salmonella SPI1 type three secretion system responds to periplasmic disulfide bond status via the flagellar apparatus and the RcsCDB system
Q43694249	The TGV transgenic vectors for single-copy gene expression from the Escherichia coli chromosome
Q37075505	The Yersinia pestis Ail protein mediates binding and Yop delivery to host cells required for plague virulence
Q28480433	The YfiBNR signal transduction mechanism reveals novel targets for the evolution of persistent Pseudomonas aeruginosa in cystic fibrosis airways
Q24606909	The ZorO-OrzO type I toxin-antitoxin locus: repression by the OrzO antitoxin
Q33899551	The adnAB locus, encoding a putative helicase-nuclease activity, is essential in Streptomyces
Q33995982	The alternative electron acceptor tetrathionate supports B12-dependent anaerobic growth of Salmonella enterica serovar typhimurium on ethanolamine or 1,2-propanediol
Q42262462	The critical role of S-lactoylglutathione formation during methylglyoxal detoxification in Escherichia coli
Q41875241	The cytoplasmic C-terminal domain of the Escherichia coli KdpD protein functions as a K+ sensor
Q36314940	The deoxycytidine pathway for thymidylate synthesis in Escherichia coli
Q36562091	The fidelity of transcription: RPB1 (RPO21) mutations that increase transcriptional slippage in S. cerevisiae.
Q47892067	The form of a trade-off determines the response to competition
Q34261523	The genomes, proteomes, and structures of three novel phages that infect the Bacillus cereus group and carry putative virulence factors
Q34090078	The global regulator RNase III modulates translation repression by the transcription elongation factor N
Q39191624	The inactivation of rfaP, rarA or sspA gene improves the viability of the Escherichia coli DNA polymerase III holD mutant
Q35215217	The involvement of replication in single stranded oligonucleotide-mediated gene repair.
Q33331400	The lambda red proteins promote efficient recombination between diverged sequences: implications for bacteriophage genome mosaicism
Q30329212	The lytB gene of Escherichia coli is essential and specifies a product needed for isoprenoid biosynthesis.
Q59125140	The lytic transglycosylase MltB connects membrane homeostasis and in vivo fitness of Acinetobacter baumannii
Q38318207	The maltodextrin system of Escherichia coli: glycogen-derived endogenous induction and osmoregulation
Q38061976	The molecular toolbox for chromosomal heterologous multiprotein expression in Escherichia coli
Q42840991	The multidrug efflux pump MdtEF protects against nitrosative damage during the anaerobic respiration in Escherichia coli.
Q28770107	The operator and early promoter region of the Shiga toxin type 2-encoding bacteriophage 933W and control of toxin expression
Q42035596	The pH dependence of the allosteric response of human liver pyruvate kinase to fructose-1,6-bisphosphate, ATP, and alanine
Q28476324	The pentameric vertex proteins are necessary for the icosahedral carboxysome shell to function as a CO2 leakage barrier
Q30476546	The pseudorabies virus VP1/2 tegument protein is required for intracellular capsid transport
Q42391802	The right half of the Escherichia coli replication origin is not essential for viability, but facilitates multi-forked replication
Q52578334	The role of complex formation between the Escherichia coli hydrogenase accessory factors HypB and SlyD.
Q35869883	The segregation of Escherichia coli minichromosomes constructed in vivo by recombineering
Q24598644	The small RNA SgrS controls sugar-phosphate accumulation by regulating multiple PTS genes
Q57059731	The small protein MgtS and small RNA MgrR modulate the PitA phosphate symporter to boost intracellular magnesium levels
Q54437426	The structure of the R184A mutant of the inositol monophosphatase encoded by suhB and implications for its functional interactions in Escherichia coli.
Q43936791	The switch from inorganic to organic sulphur assimilation in Escherichia coli: adenosine 5'-phosphosulphate (APS) as a signalling molecule for sulphate excess
Q40497047	The terminal 5' phosphate and proximate phosphorothioate promote ligation-independent cloning
Q35596402	The trans-envelope Tol-Pal complex is part of the cell division machinery and required for proper outer-membrane invagination during cell constriction in E. coli
Q41973368	The transcriptional factors MurR and catabolite activator protein regulate N-acetylmuramic acid catabolism in Escherichia coli
Q27931264	The universal YrdC/Sua5 family is required for the formation of threonylcarbamoyladenosine in tRNA.
Q34977384	The yggH Gene of Escherichia coli Encodes a tRNA (m 7 G46) Methyltransferase
Q40345257	The β-glucoside (bgl) operon of Escherichia coli is involved in the regulation of oppA, encoding an oligopeptide transporter
Q35913011	Thioesterase superfamily member 2/acyl-CoA thioesterase 13 (Them2/Acot13) regulates hepatic lipid and glucose metabolism
Q34177325	Three Yersinia pestis Adhesins Facilitate Yop Delivery to Eukaryotic Cells and Contribute to Plague Virulence
Q39679916	Tools for characterization of Escherichia coli genes of unknown function
Q36661286	Topological domain structure of the Escherichia coli chromosome
Q30719533	Towards genetic genome projects: genomic library screening and gene-targeting vector construction in a single step
Q38315295	Transcript analysis reveals an extended regulon and the importance of protein-protein co-operativity for the Escherichia coli methionine repressor
Q35879284	Transcription antitermination by translation initiation factor IF1.
Q50116192	Transcription induces a supercoil domain barrier in bacteriophage Mu.
Q58751695	Transcriptional Regulation Buffers Gene Dosage Effects on a Highly Expressed Operon in
Q38290010	Transcriptional and post-transcriptional regulation of histone variant H2A.Z during sea urchin development
Q34697291	Transcriptional and translational control of the Salmonella fliC gene
Q39735950	Transcriptional and translational dual-regulated oncolytic herpes simplex virus type 1 for targeting prostate tumors
Q34310092	Transcriptional interference by a complex formed at the centromere-like partition site of plasmid P1.
Q42018354	Transcriptional pausing at the translation start site operates as a critical checkpoint for riboswitch regulation
Q40947162	Transcriptional regulation of sitABCD of Salmonella enterica serovar Typhimurium by MntR and Fur
Q21563354	Translation enhancing ACA motifs and their silencing by a bacterial small regulatory RNA
Q33878214	Translational control and Rho-dependent transcription termination are intimately linked in riboswitch regulation.
Q52401284	Translational regulation by bacterial small RNAs via an unusual Hfq-dependent mechanism.
Q35879388	Translational regulation of the Escherichia coli stress factor RpoS: a role for SsrA and Lon
Q46680790	Transposition is modulated by a diverse set of host factors in Escherichia coli and is stimulated by nutritional stress
Q33525588	Transposon assisted gene insertion technology (TAGIT): a tool for generating fluorescent fusion proteins
Q51839435	Transposon-mediated BAC transgenesis in zebrafish.
Q35652456	Trigger Factor can antagonize both SecB and DnaK/DnaJ chaperone functions in Escherichia coli.
Q49985474	Turnover of mRNAs is one of the essential functions of RNase E.
Q63616086	Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye and Brain, Including Seventeen Carrying a New Inducible-First Constitutive-Ready Allele
Q24633959	Two antisense RNAs target the transcriptional regulator CsgD to inhibit curli synthesis
Q41964800	Two gamma interferon-activated site-like elements in the human cytomegalovirus major immediate-early promoter/enhancer are important for viral replication.
Q39083061	Two regulatory RNA elements affect TisB-dependent depolarization and persister formation.
Q33948064	Two ways to trap a gene in mice
Q42773641	Ubiquitous and uniform in vivo fluorescence in ROSA26-EGFP BAC transgenic mice
Q34995724	Understanding the enormous diversity of bacteriophages: the tailed phages that infect the bacterial family Enterobacteriaceae
Q46522626	Unstable tandem gene amplification generates heteroresistance (variation in resistance within a population) to colistin in Salmonella enterica
Q42660647	Use of a riboswitch-controlled conditional hypomorphic mutation to uncover a role for the essential csrA gene in bacterial autoaggregation.
Q46903401	Use of gene-manipulated mice in the study of erythropoietin gene expression
Q42257417	Use of recombination-mediated genetic engineering for construction of rescue human cytomegalovirus bacterial artificial chromosome clones
Q33434157	Use of the lambda Red-recombineering method for genetic engineering of Pantoea ananatis
Q35748055	Use of transposase and ends of IS608 enables precise and scarless genome modification for modulating gene expression and metabolic engineering applications in Escherichia coli
Q54324507	Using recombineering to generate point mutations: the oligonucleotide-based "hit and fix" method.
Q54342427	Using recombineering to generate point mutations:galK-based positive-negative selection method.
Q36302456	UvrD Participation in Nucleotide Excision Repair Is Required for the Recovery of DNA Synthesis following UV-Induced Damage in Escherichia coli
Q35162297	Validation of a fluorescence-based screening concept to identify ribosome assembly defects in Escherichia coli
Q40726188	Visual impairment in an optineurin mouse model of primary open-angle glaucoma.
Q35804553	Visualizing the proteome of Escherichia coli: an efficient and versatile method for labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes
Q42723133	Why does Escherichia coli grow more slowly on glucosamine than on N-acetylglucosamine? Effects of enzyme levels and allosteric activation of GlcN6P deaminase (NagB) on growth rates
Q45303717	Wild-type huntingtin participates in protein trafficking between the Golgi and the extracellular space
Q34004834	YbdG in Escherichia coli is a threshold-setting mechanosensitive channel with MscM activity
Q27313886	YbiB from Escherichia coli, the Defining Member of the Novel TrpD2 Family of Prokaryotic DNA-binding Proteins
Q33543125	YfiBNR mediates cyclic di-GMP dependent small colony variant formation and persistence in Pseudomonas aeruginosa
Q30159740	YfiO stabilizes the YaeT complex and is essential for outer membrane protein assembly in Escherichia coli
Q35759344	YjjG, a dUMP phosphatase, is critical for thymine utilization by Escherichia coli K-12
Q42205921	YtqI from Bacillus subtilis has both oligoribonuclease and pAp-phosphatase activity
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P819	ADS bibcode	2000PNAS...97.5978Y
P356	DOI	10.1073/PNAS.100127597
P3181	OpenCitations bibliographic resource ID	908476
P932	PMC publication ID	18544
P698	PubMed publication ID	10811905
P5875	ResearchGate publication ID	12505760

P2093	author name string	Jenkins NA
		Copeland NG
		Yu D
		Court DL
		Lee EC
		Ellis HM
P2860	cites work	Transfection of Escherichia coli spheroplasts. V. Activity of recBC nuclease in rec+ and rec minus spheroplasts measured with different forms of bacteriophage DNA	Q30450610
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P433	issue	11
P407	language of work or name	English	Q1860
P921	main subject	Escherichia coli	Q25419
P304	page(s)	5978-83
P577	publication date	2000-05-23
P1433	published in	Proceedings of the National Academy of Sciences of the United States of America	Q1146531
P1476	title	An efficient recombination system for chromosome engineering in Escherichia coli
P478	volume	97

An efficient recombination system for chromosome engineering in Escherichia coli

scientific article (publication date: 23 May 2000)

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