Cloning Should Be Simple: Escherichia coli DH5α-Mediated Assembly of Multiple DNA Fragments with Short End Homologies

scientific article published on 8 September 2015

Cloning Should Be Simple: Escherichia coli DH5α-Mediated Assembly of Multiple DNA Fragments with Short End Homologies is …
instance of (P31):
scholarly articleQ13442814

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P819ADS bibcode2015PLoSO..1037466K
P356DOI10.1371/JOURNAL.PONE.0137466
P932PMC publication ID4562628
P698PubMed publication ID26348330
P5875ResearchGate publication ID281621602

P2093author name stringRuth E Richardson
Maxim Kostylev
Yo Suzuki
Anne E Otwell
P2860cites workOligonucleotide recombination in Gram-negative bacteriaQ36114524
Generation of cohesive ends on PCR products by UDG-mediated excision of dU, and application for cloning into restriction digest-linearized vectorsQ36764319
In vivo cloning of PCR products in E. coliQ40412972
Rapid cloning by homologous recombination in vivoQ40519954
Simple cloning via direct transformation of PCR product (DNA Multimer) to Escherichia coli and Bacillus subtilisQ42600880
Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting.Q45381468
Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC.Q51066780
Homologous recombinatorial cloning without the creation of single-stranded ends: exonuclease and ligation-independent cloning (ELIC).Q54186281
Rapid assembly of multiple DNA fragments through direct transformation of PCR products into E. coli and Lactobacillus.Q54190624
In vivo intermolecular recombination in Escherichia coli: application to plasmid constructions.Q54590198
Plasmid construction by homologous recombination in yeast.Q54771077
Recombineering: genetic engineering in bacteria using homologous recombinationQ87682799
Circular polymerase extension cloning of complex gene libraries and pathwaysQ21090055
One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genomeQ22066288
Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systemsQ24633670
Biochemistry of homologous recombination in Escherichia coliQ24634614
Studies on transformation of Escherichia coli with plasmidsQ27860598
The double-strand-break repair model for recombinationQ28267259
Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extensionQ29547330
An efficient recombination system for chromosome engineering in Escherichia coliQ29615038
A new logic for DNA engineering using recombination in Escherichia coliQ29615202
High efficiency transformation of E. coli by high voltage electroporationQ29615278
CRISPR-Cas systems for editing, regulating and targeting genomesQ29615781
Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics effortsQ29619067
Versatile genetic assembly system (VEGAS) to assemble pathways for expression in S. cerevisiaeQ30371791
FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning methodQ31033460
A genetic system for direct selection of gene-positive clones during recombinational cloning in yeastQ32058707
An end-joining repair mechanism in Escherichia coliQ33719661
Population structure of KPC-producing Klebsiella pneumoniae isolates from midwestern U.S. hospitalsQ34058131
Enzymatic assembly of overlapping DNA fragmentsQ34186536
Crossing over between regions of limited homology in Escherichia coli. RecA-dependent and RecA-independent pathwaysQ34614694
DNA fragments assembly based on nicking enzyme systemQ34618467
Knocking out multigene redundancies via cycles of sexual assortment and fluorescence selectionQ34793244
Rapid metabolic pathway assembly and modification using serine integrase site-specific recombinationQ35041926
A condition-specific codon optimization approach for improved heterologous gene expression in Saccharomyces cerevisiaeQ35122433
Optimal cloning of PCR fragments by homologous recombination in Escherichia coli.Q35181472
RecA-independent recombination is efficient but limited by exonucleasesQ35575939
The insertion Green Monster (iGM) method for expression of multiple exogenous genes in yeastQ35683641
SLiCE: a novel bacterial cell extract-based DNA cloning methodQ35906084
P275copyright licenseCreative Commons Attribution 4.0 InternationalQ20007257
P6216copyright statuscopyrightedQ50423863
P433issue9
P407language of work or nameEnglishQ1860
P921main subjectEscherichia coliQ25419
P304page(s)e0137466
P577publication date2015-09-08
P1433published inPLOS OneQ564954
P1476titleCloning Should Be Simple: Escherichia coli DH5α-Mediated Assembly of Multiple DNA Fragments with Short End Homologies
P478volume10

Reverse relations

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