| scholarly article | Q13442814 |
| P2093 | author name string | Uwe Werling | |
| Winfried Edelmann | |||
| Yongwei Zhang | |||
| P2860 | cites work | Construction of Biologically Functional Bacterial Plasmids In Vitro | Q24562887 |
| Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae | Q24616257 | ||
| Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter | Q27860697 | ||
| Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome | Q28265807 | ||
| An efficient recombination system for chromosome engineering in Escherichia coli | Q29615038 | ||
| A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA | Q29615197 | ||
| A new logic for DNA engineering using recombination in Escherichia coli | Q29615202 | ||
| In-fusion assembly: seamless engineering of multidomain fusion proteins, modular vectors, and mutations. | Q50899734 | ||
| Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC. | Q51066780 | ||
| Mechanisms of recombination: lessons from E. coli | Q82640171 | ||
| Ribocloning: DNA cloning and gene construction using PCR primers terminated with a ribonucleotide | Q83834563 | ||
| Ligation-independent cloning of PCR products (LIC-PCR) | Q29618962 | ||
| Recombinational repair of DNA damage in Escherichia coli and bacteriophage lambda | Q29619755 | ||
| A restriction enzyme from Hemophilus influenzae. I. Purification and general properties | Q34053577 | ||
| An exonuclease induced by bacteriophage lambda. II. Nature of the enzymatic reaction | Q34243330 | ||
| Crossing over between regions of limited homology in Escherichia coli. RecA-dependent and RecA-independent pathways | Q34614694 | ||
| RecE/RecT and Redalpha/Redbeta initiate double-stranded break repair by specifically interacting with their respective partners | Q35200055 | ||
| RecA-independent recombination is efficient but limited by exonucleases | Q35575939 | ||
| Genetic recombination of bacterial plasmid DNA: electron microscopic analysis of in vitro intramolecular recombination | Q36403105 | ||
| Duplex strand joining reactions catalyzed by vaccinia virus DNA polymerase | Q38306678 | ||
| Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli | Q39565526 | ||
| Rapid modification of bacterial artificial chromosomes by ET-recombination. | Q39727582 | ||
| Enzyme-free cloning: a rapid method to clone PCR products independent of vector restriction enzyme sites | Q39729098 | ||
| Construction of recombinant DNA by exonuclease recession | Q40406576 | ||
| Exonuclease III induced ligase-free directional subcloning of PCR products | Q40412789 | ||
| The role of exonuclease and beta protein of phage lambda in genetic recombination. 3. Binding to deoxyribonucleic acid | Q43814632 | ||
| The role of exonuclease and beta protein of phage lambda in genetic recombination. II. Substrate specificity and the mode of action of lambda exonuclease. | Q47756632 | ||
| Scarless engineering of the Escherichia coli genome. | Q47790748 | ||
| P275 | copyright license | Creative Commons Attribution-NonCommercial 3.0 Unported | Q18810331 |
| P6216 | copyright status | copyrighted | Q50423863 |
| P433 | issue | 8 | |
| P407 | language of work or name | English | Q1860 |
| P304 | page(s) | e55 | |
| P577 | publication date | 2012-01-12 | |
| P1433 | published in | Nucleic Acids Research | Q135122 |
| P1476 | title | SLiCE: a novel bacterial cell extract-based DNA cloning method | |
| P478 | volume | 40 |
| Q35860677 | A 3D Searchable Database of Transgenic Zebrafish Gal4 and Cre Lines for Functional Neuroanatomy Studies |
| Q35692625 | A CRISPR-Cas9 System for Genetic Engineering of Filamentous Fungi |
| Q36134059 | A High Through-put Platform for Recombinant Antibodies to Folded Proteins |
| Q47875405 | A MYB-type transcription factor, MYB2, represses light-harvesting protein genes in Cyanidioschyzon merolae. |
| Q46286217 | A Mutator Phenotype Promoting the Emergence of Spontaneous Oxidative Stress-Resistant Mutants in Campylobacter jejuni |
| Q88506016 | A Saturation Mutagenesis Approach to Understanding PTEN Lipid Phosphatase Activity and Genotype-Phenotype Relationships |
| Q55314026 | A direct regulatory link between microRNA-137 and SHANK2: implications for neuropsychiatric disorders. |
| Q35097335 | A modular plasmid assembly kit for multigene expression, gene silencing and silencing rescue in plants |
| Q54202312 | A multi-phenotypic imaging screen to identify bacterial effectors by exogenous expression in a HeLa cell line. |
| Q37197853 | A new strategy for gene targeting and functional proteomics using the DT40 cell line |
| Q35641856 | A novel and simple method for rapid generation of recombinant porcine adenoviral vectors for transgene expression |
| Q37166976 | A point mutation in AgrC determines cytotoxic or colonizing properties associated with phenotypic variants of ST22 MRSA strains |
| Q47716927 | A sigma factor toolbox for orthogonal gene expression in Escherichia coli. |
| Q35650381 | A simple and efficient seamless DNA cloning method using SLiCE from Escherichia coli laboratory strains and its application to SLiP site-directed mutagenesis |
| Q47124920 | A simple and ultra-low cost homemade seamless ligation cloning extract (SLiCE) as an alternative to a commercially available seamless DNA cloning kit. |
| Q34992142 | A simplified positive-sense-RNA virus construction approach that enhances analysis throughput |
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| Q28681353 | A survey of enabling technologies in synthetic biology |
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| Q22065908 | Bacillus subtilis and Escherichia coli essential genes and minimal cell factories after one decade of genome engineering |
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| Q64102380 | CAV-2 Vector Development and Gene Transfer in the Central and Peripheral Nervous Systems |
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| Q92733592 | COMPASS for rapid combinatorial optimization of biochemical pathways based on artificial transcription factors |
| Q92956560 | COSPLAY: An expandable toolbox for combinatorial and swift generation of expression plasmids in yeast |
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| Q35766713 | Cloning Should Be Simple: Escherichia coli DH5α-Mediated Assembly of Multiple DNA Fragments with Short End Homologies |
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| Q35918607 | Comparison and optimization of ten phage encoded serine integrases for genome engineering in Saccharomyces cerevisiae |
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| Q59647553 | Comprehensive antibiotic-linked mutation assessment by resistance mutation sequencing (RM-seq) |
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| Q48505745 | Convergent Evolution Driven by Rifampin Exacerbates the Global Burden of Drug-Resistant Staphylococcus aureus. |
| Q37637838 | Correspondence: Spontaneous secondary mutations confound analysis of the essential two-component system WalKR in Staphylococcus aureus |
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| Q35017533 | DNA assembly techniques for next-generation combinatorial biosynthesis of natural products |
| Q94948782 | Design principles of a minimal auxin response system |
| Q28651359 | Developments in the tools and methodologies of synthetic biology |
| Q35007311 | Directional cloning of DNA fragments using deoxyinosine-containing oligonucleotides and endonuclease V. |
| Q93067775 | Domain swaps of Arabidopsis secondary wall cellulose synthases to elucidate their class specificity |
| Q48587042 | EXO1 suppresses double-strand break induced homologous recombination between diverged sequences in mammalian cells |
| Q48147201 | Efficient strategy for introducing large and multiple changes in plasmid DNA. |
| Q38109520 | Emerging tools for synthetic genome design |
| Q63987403 | Engram-specific transcriptome profiling of contextual memory consolidation |
| Q35206838 | Ex vivo DNA Assembly |
| Q64253364 | Exonuclease III (XthA) Enforces DNA Cloning of Escherichia coli To Create Cohesive Ends |
| Q34987765 | Expression-level optimization of a multi-enzyme pathway in the absence of a high-throughput assay |
| Q64882409 | Fab is the most efficient format to express functional antibodies by yeast surface display. |
| Q36296236 | Generating Conformation and Complex-Specific Synthetic Antibodies |
| Q56889298 | Generating FN3-Based Affinity Reagents Through Phage Display |
| Q39438092 | Genetic prevention of hepatitis C virus-induced liver fibrosis by allele-specific downregulation of MERTK. |
| Q38603581 | Genome scale engineering techniques for metabolic engineering |
| Q22122302 | Genome-scale engineering for systems and synthetic biology |
| Q57823035 | Global spread of three multidrug-resistant lineages of Staphylococcus epidermidis |
| Q38710090 | Glutamate‑mediated effects of caffeine and interferon‑γ on mercury-induced toxicity |
| Q38728295 | High molecular weight DNA assembly in vivo for synthetic biology applications |
| Q28066811 | High-throughput recombinant protein expression in Escherichia coli: current status and future perspectives |
| Q35193924 | HomeRun Vector Assembly System: a flexible and standardized cloning system for assembly of multi-modular DNA constructs |
| Q90700927 | How metal cofactors drive dimer-dodecamer transition of the M42 aminopeptidase TmPep1050 of Thermotoga maritima |
| Q35268718 | IRDL cloning: a one-tube, zero-background, easy-to-use, directional cloning method improves throughput in recombinant DNA preparation |
| Q36041542 | IVA cloning: A single-tube universal cloning system exploiting bacterial In Vivo Assembly |
| Q36301065 | Identification and functional characterization of rare SHANK2 variants in schizophrenia |
| Q64058785 | Identification of a -Like Gene Encoding an Endonuclease III in |
| Q48230382 | In silico identification and experimental validation of amino acid motifs required for the Rho-of-plants GTPase-mediated activation of receptor-like cytoplasmic kinases. |
| Q41487777 | In vivo Assembly in Escherichia coli of Transformation Vectors for Plastid Genome Engineering |
| Q90110844 | In vivo DNA assembly using common laboratory bacteria: A re-emerging tool to simplify molecular cloning |
| Q41512624 | In vivo cloning of up to 16 kb plasmids in E. coli is as simple as PCR. |
| Q90047029 | Intra-Molecular Homologous Recombination of Scarless Plasmid |
| Q34243738 | Inverse fusion PCR cloning |
| Q37571415 | Kinetochore assembly and heterochromatin formation occur autonomously in Schizosaccharomyces pombe |
| Q31151104 | Knockin' on pHeaven's door: a fast and reliable high-throughput compatible zero-background cloning procedure. |
| Q54981009 | L-SCRaMbLE as a tool for light-controlled Cre-mediated recombination in yeast. |
| Q35585253 | Lambda red mediated gap repair utilizes a novel replicative intermediate in Escherichia coli |
| Q35660132 | Large Scale Bacterial Colony Screening of Diversified FRET Biosensors |
| Q34417984 | Large-scale de novo DNA synthesis: technologies and applications |
| Q48042622 | Longevity in vivo of primary cell wall cellulose synthases. |
| Q41861467 | Looking beyond GWAS: allele-specific transcription factor binding drives the association of GALNT2 to HDL-C plasma levels |
| Q34871025 | Massively parallel decoding of mammalian regulatory sequences supports a flexible organizational model |
| Q38768911 | Mechanism of bacterial gene rearrangement: SprA-catalyzed precise DNA recombination and its directionality control by SprB ensure the gene rearrangement and stable expression of spsM during sporulation in Bacillus subtilis. |
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| Q64283743 | Memory strength gates the involvement of a CREB-dependent cortical fear engram in remote memory |
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| Q64964476 | MgrA Negatively Regulates Biofilm Formation and Detachment by Repressing the Expression of psm Operons in Staphylococcus aureus. |
| Q92180771 | Mice deficient in the C-terminal domain of TAR DNA-binding protein 43 develop age-dependent motor dysfunction associated with impaired Notch1-Akt signaling pathway |
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| Q36075996 | Modulation of ccrAB Expression and SCCmec Excision by an Inverted Repeat Element and SarS in Methicillin-Resistant Staphylococcus aureus |
| Q50088071 | Multiple environmental factors influence the importance of the phosphodiesterase DipA upon Pseudomonas aeruginosa swarming. |
| Q57822413 | Mutational analysis of the conserved carboxylates of anion channelrhodopsin-2 (ACR2) expressed in and their roles in anion transport |
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