High-Efficiency Multi-site Genomic Editing of Pseudomonas putida through Thermoinducible ssDNA Recombineering

scientific article published on 26 February 2020

High-Efficiency Multi-site Genomic Editing of Pseudomonas putida through Thermoinducible ssDNA Recombineering is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1016/J.ISCI.2020.100946
P932PMC publication ID7068128
P698PubMed publication ID32179472

P50authorÁkos NyergesQ42738850
P2093author name stringEsteban Martínez-García
Víctor de Lorenzo
Tomas Aparicio
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Scarless and sequential gene modification in Pseudomonas using PCR product flanked by short homology regionsQ33648542
High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotidesQ33948878
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CRMAGE: CRISPR Optimized MAGE RecombineeringQ36500383
Automated design of synthetic ribosome binding sites to control protein expressionQ37436696
Determination of the optimal aligned spacing between the Shine-Dalgarno sequence and the translation initiation codon of Escherichia coli mRNAsQ37587211
Conditional DNA repair mutants enable highly precise genome engineeringQ37734351
Biotechnological domestication of pseudomonads using synthetic biology.Q38204897
From dirt to industrial applications: Pseudomonas putida as a Synthetic Biology chassis for hosting harsh biochemical reactionsQ38848033
The Ssr protein (T1E_1405) from Pseudomonas putida DOT-T1E enables oligonucleotide-based recombineering in platform strain P. putida EM42.Q39639221
Pseudomonas 2.0: genetic upgrading of P. putida KT2440 as an enhanced host for heterologous gene expression.Q41914986
Detection of novel recombinases in bacteriophage genomes unveils Rad52, Rad51 and Gp2.5 remote homologs.Q42060342
A standardized workflow for surveying recombinases expands bacterial genome-editing capabilities.Q46270330
Toward Genome-Based Metabolic Engineering in BacteriaQ47428632
Pseudomonas putida KT2440 markerless gene deletion using a combination of λ Red recombineering and Cre/loxP site-specific recombinationQ48065683
Design, synthesis, and testing toward a 57-codon genome.Q48687337
Directed evolution of multiple genomic loci allows the prediction of antibiotic resistance.Q55348060
CRISPR/Cas9-based Genome Editing in Pseudomonas aeruginosa and Cytidine Deaminase-Mediated Base Editing in Pseudomonas SpeciesQ58804345
Single-Stranded DNA-Binding Protein and Exogenous RecBCD Inhibitors Enhance Phage-Derived Homologous Recombination in PseudomonasQ64103429
Crystal structure of the Redβ C-terminal domain in complex with λ Exonuclease reveals an unexpected homology with λ Orf and an interaction with Escherichia coli single stranded DNA binding proteinQ64231038
A Broad Host Range Plasmid-Based Roadmap for ssDNA-Based Recombineering in Gram-Negative BacteriaQ90477971
Mismatch repair hierarchy of Pseudomonas putida revealed by mutagenic ssDNA recombineering of the pyrF geneQ90624154
Multiple-Site Diversification of Regulatory Sequences Enables Interspecies Operability of Genetic DevicesQ91635832
Pseudomonas putida in the quest of programmable chemistryQ91708880
Improved Thermotolerance of Genome-Reduced Pseudomonas putida EM42 Enables Effective Functioning of the PL /cI857 SystemQ92999067
Rapid Evolution of Reduced Susceptibility against a Balanced Dual-Targeting Antibiotic through Stepping-Stone MutationsQ92999174
P433issue3
P921main subjectPseudomonas putidaQ2738168
P304page(s)100946
P577publication date2020-02-26
P1476titleHigh-Efficiency Multi-site Genomic Editing of Pseudomonas putida through Thermoinducible ssDNA Recombineering
P478volume23

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cites work (P2860)
Q100750162Exploring the synthetic biology potential of bacteriophages for engineering non-model bacteria
Q100958556Genome-scale metabolic rewiring improves titers rates and yields of the non-native product indigoidine at scale

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