| scholarly article | Q13442814 |
| P50 | author | George M. Church | Q3298995 |
| Andrew C Tolonen | Q42338586 | ||
| Michael C Jewett | Q58326824 | ||
| Daniel B Goodman | Q58443240 | ||
| Marc J. Lajoie | Q63679014 | ||
| P2093 | author name string | Duhee Bang | |
| Nikos B Reppas | |||
| Tara A Gianoulis | |||
| Laurens Kraal | |||
| Peter A Carr | |||
| Harris H Wang | |||
| Farren J Isaacs | |||
| Joseph M Jacobson | |||
| Bram Sterling | |||
| Christopher J Emig | |||
| Samuel J Hwang | |||
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| P433 | issue | 6040 | |
| P407 | language of work or name | English | Q1860 |
| P304 | page(s) | 348-353 | |
| P577 | publication date | 2011-07-01 | |
| P1433 | published in | Science | Q192864 |
| P1476 | title | Precise manipulation of chromosomes in vivo enables genome-wide codon replacement | |
| P478 | volume | 333 |
| Q48143162 | "Perfect" designer chromosome V and behavior of a ring derivative |
| Q34588001 | A Heritable Recombination system for synthetic Darwinian evolution in yeast |
| Q35859249 | A Tool for Multiple Targeted Genome Deletions that Is Precise, Scar-Free, and Suitable for Automation |
| Q47219915 | A brief history of synthetic biology |
| Q28267103 | A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation |
| Q35925574 | A highly precise and portable genome engineering method allows comparison of mutational effects across bacterial species |
| Q35152640 | A simple and effective method for construction of Escherichia coli strains proficient for genome engineering |
| Q46270330 | A standardized workflow for surveying recombinases expands bacterial genome-editing capabilities. |
| Q28681353 | A survey of enabling technologies in synthetic biology |
| Q64120805 | A versatile platform strain for high-fidelity multiplex genome editing |
| Q74441586 | A yeast for all reasons |
| Q36470309 | Accelerated genome engineering through multiplexing |
| Q90425917 | Active Targeting of Cancer Cells by Nanobody Decorated Polypeptide Micelle with Bio-orthogonally Conjugated Drug |
| Q30373900 | An ancient Chinese wisdom for metabolic engineering: Yin-Yang. |
| Q38289079 | Automated electrotransformation of Escherichia coli on a digital microfluidic platform using bioactivated magnetic beads |
| Q57970482 | Auxotrophy to Xeno-DNA: an exploration of combinatorial mechanisms for a high-fidelity biosafety system for synthetic biology applications |
| Q37645309 | Bacterial cellular engineering by genome editing and gene silencing |
| Q34017160 | Bacteriophage-based synthetic biology for the study of infectious diseases. |
| Q47324234 | Beyond editing to writing large genomes |
| Q26744578 | Bifidobacteria and Their Role as Members of the Human Gut Microbiota |
| Q28255773 | Biocontainment of genetically modified organisms by synthetic protein design |
| Q38244615 | Biological applications of expanded genetic codes |
| Q26753076 | Biosensor-based engineering of biosynthetic pathways |
| Q38125191 | Bridging the gap between systems biology and synthetic biology. |
| Q103836662 | Building genomes to understand biology |
| Q57818221 | CRISPR-Cas9/Cas12a biotechnology and application in bacteria |
| Q34045724 | CRISPR/Cas9 advances engineering of microbial cell factories. |
| Q90350496 | CRISPR/Cas9 recombineering-mediated deep mutational scanning of essential genes in Escherichia coli |
| Q47421848 | CRISPR/Cas9-Based Counterselection Boosts Recombineering Efficiency in Pseudomonas putida. |
| Q47875129 | Cell engineering: How to hack the genome |
| Q27004476 | Cell-free biology: exploiting the interface between synthetic biology and synthetic chemistry |
| Q36909733 | Cell-free co-production of an orthogonal transfer RNA activates efficient site-specific non-natural amino acid incorporation. |
| Q35878115 | Cell-free synthetic biology: thinking outside the cell |
| Q45072574 | Challenges and Advances for Genetic Engineering of Non-model Bacteria and Uses in Consolidated Bioprocessing |
| Q62671640 | Chemical synthesis rewriting of a bacterial genome to achieve design flexibility and biological functionality |
| Q40639087 | Codon usage in Alphabaculovirus and Betabaculovirus hosted by the same insect species is weak, selection dominated and exhibits no more similar patterns than expected |
| Q88595821 | Codon usage of highly expressed genes affects proteome-wide translation efficiency |
| Q37654825 | Combinatorial approaches for inverse metabolic engineering applications. |
| Q37734351 | Conditional DNA repair mutants enable highly precise genome engineering |
| Q28083947 | Contemplating 3-Hydroxypropionic Acid Biosynthesis in Klebsiella pneumoniae |
| Q34135500 | Cost-effective, high-throughput DNA sequencing libraries for multiplexed target capture |
| Q38014801 | Crosstalk between endogenous and synthetic components--synthetic signaling meets endogenous components |
| Q36179505 | DNA synthesis, assembly and applications in synthetic biology |
| Q58396289 | DNA technology and evolution of the Central Dogma |
| Q47389348 | Design and chemical synthesis of eukaryotic chromosomes |
| Q28708939 | Design and development of synthetic microbial platform cells for bioenergy |
| Q48143131 | Design of a synthetic yeast genome. |
| Q54243474 | Design, development and application of whole-cell based antibiotic-specific biosensor. |
| Q39065918 | Designing logical codon reassignment - Expanding the chemistry in biology |
| Q28651359 | Developments in the tools and methodologies of synthetic biology |
| Q35625131 | Differential requirements of singleplex and multiplex recombineering of large DNA constructs |
| Q35132236 | Directed Evolution: Past, Present and Future |
| Q26823491 | Directed evolution combined with synthetic biology strategies expedite semi-rational engineering of genes and genomes |
| Q26823011 | Directed evolution: an evolving and enabling synthetic biology tool |
| Q59810136 | Dissecting the Contribution of Release Factor Interactions to Amber Stop Codon Reassignment Efficiencies of the Orthogonal Pair |
| Q36728530 | Efficient Reassignment of a Frequent Serine Codon in Wild-Type Escherichia coli |
| Q42152529 | Efficient decoding of the UAG triplet as a full-fledged sense codon enhances the growth of a prfA-deficient strain of Escherichia coli |
| Q42364606 | Efficient engineering of chromosomal ribosome binding site libraries in mismatch repair proficient Escherichia coli. |
| Q37281621 | Emergent rules for codon choice elucidated by editing rare arginine codons in Escherichia coli |
| Q33808968 | Emerging strategies and integrated systems microbiology technologies for biodiscovery of marine bioactive compounds. |
| Q56906823 | Encoding human serine phosphopeptides in bacteria for proteome-wide identification of phosphorylation-dependent interactions |
| Q38259168 | Engineering allostery |
| Q38009596 | Engineering and control of biological systems: A new way to tackle complex diseases. |
| Q60045721 | Engineering and modification of microbial chassis for systems and synthetic biology |
| Q54337934 | Engineering glyceraldehyde-3-phosphate dehydrogenase for switching control of glycolysis in Escherichia coli. |
| Q89902832 | Engineering the Translational Machinery for Biotechnology Applications |
| Q36280579 | Enhanced multiplex genome engineering through co-operative oligonucleotide co-selection |
| Q36323009 | Enhanced phosphoserine insertion during Escherichia coli protein synthesis via partial UAG codon reassignment and release factor 1 deletion |
| Q64067575 | Enzyme promiscuity shapes adaptation to novel growth substrates |
| Q54204592 | Escherichia coli as a host for metabolic engineering. |
| Q36669000 | Evolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids. |
| Q49831017 | Evolving Bacterial Fitness with an Expanded Genetic Code. |
| Q40696654 | Evolving tRNA(Sec) for efficient canonical incorporation of selenocysteine |
| Q27678699 | Expanded use of sense codons is regulated by modified cytidines in tRNA |
| Q28246486 | Expanding the genetic code of Escherichia coli with phosphoserine |
| Q28303893 | Experimental challenges of sense codon reassignment: an innovative approach to genetic code expansion |
| Q44331574 | Experimental evolution of a green fluorescent protein composed of 19 unique amino acids without tryptophan. |
| Q55452282 | Far rapid synthesis of giant DNA in the Bacillus subtilis genome by a conjugation transfer system. |
| Q38750016 | Future of the Genetic Code |
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| Q50228413 | Genetic Code Expansion by Degeneracy Reprogramming of Arginyl Codons |
| Q36462825 | Genetic code flexibility in microorganisms: novel mechanisms and impact on physiology. |
| Q34457705 | Genetically encoded libraries of nonstandard peptides |
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| Q45736002 | Genome remodeling |
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| Q93052007 | Genome-driven cell engineering review: in vivo and in silico metabolic and genome engineering |
| Q22122302 | Genome-scale engineering for systems and synthetic biology |
| Q34224602 | Genome-scale promoter engineering by coselection MAGE |
| Q28539258 | Genome-wide mapping of furfural tolerance genes in Escherichia coli |
| Q36219845 | Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering. |
| Q33834503 | Genomes by design |
| Q40646957 | Genomic Recoding Broadly Obstructs the Propagation of Horizontally Transferred Genetic Elements |
| Q28300435 | Genomically recoded organisms expand biological functions |
| Q36660664 | Genotyping and genomic profiling of non-small-cell lung cancer: implications for current and future therapies |
| Q38728295 | High molecular weight DNA assembly in vivo for synthetic biology applications |
| Q90369297 | High-Efficiency Multi-site Genomic Editing of Pseudomonas putida through Thermoinducible ssDNA Recombineering |
| Q29036647 | High-Yield, Zero-Leakage Expression System with a Translational Switch Using Site-Specific Unnatural Amino Acid Incorporation |
| Q40765704 | Highly reproductive Escherichia coli cells with no specific assignment to the UAG codon |
| Q89458516 | Improved sgRNA design in bacteria via genome-wide activity profiling |
| Q34408253 | Improving lambda red genome engineering in Escherichia coli via rational removal of endogenous nucleases |
| Q38588687 | Incorporation of Non-Canonical Amino Acids |
| Q40469827 | Integrating artificial with natural cells to translate chemical messages that direct E. coli behaviour |
| Q35090071 | Intrinsic biocontainment: multiplex genome safeguards combine transcriptional and recombinational control of essential yeast genes |
| Q35585253 | Lambda red mediated gap repair utilizes a novel replicative intermediate in Escherichia coli |
| Q40950520 | Large-scale recoding of a bacterial genome by iterative recombineering of synthetic DNA. |
| Q34145852 | Less can be more: RNA-adapters may enhance coding capacity of replicators |
| Q35578593 | Liquid-based iterative recombineering method tolerant to counter-selection escapes |
| Q36273504 | Low escape-rate genome safeguards with minimal molecular perturbation of Saccharomyces cerevisiae |
| Q36478103 | Manipulating replisome dynamics to enhance lambda Red-mediated multiplex genome engineering. |
| Q52423793 | Metabolic engineering of Escherichia coli using CRISPR-Cas9 meditated genome editing. |
| Q33730850 | Millstone: software for multiplex microbial genome analysis and engineering |
| Q38264064 | Modern and simple construction of plasmid: saving time and cost |
| Q47387337 | Modern methods for laboratory diversification of biomolecules |
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| Q54343074 | Multiple-site labeling of proteins with unnatural amino acids. |
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| Q36039000 | Multiplexed in vivo His-tagging of enzyme pathways for in vitro single-pot multienzyme catalysis |
| Q37416985 | Natural reassignment of CUU and CUA sense codons to alanine in Ashbya mitochondria |
| Q41511627 | Near-cognate suppression of amber, opal and quadruplet codons competes with aminoacyl-tRNAPyl for genetic code expansion. |
| Q56889444 | Next-generation genetic code expansion |
| Q26822920 | Non-Standard Genetic Codes Define New Concepts for Protein Engineering |
| Q38792216 | Novel Technologies for Optimal Strain Breeding |
| Q26778792 | Oligonucleotide-directed mutagenesis for precision gene editing |
| Q92343837 | On a Robust, Sensitive Cell-Free Method for Pseudomonas Sensing and Quantification in Microfluidic Templated Hydrogels |
| Q35541257 | On the road to synthetic life: the minimal cell and genome-scale engineering |
| Q41987306 | Optimizing complex phenotypes through model-guided multiplex genome engineering |
| Q58094703 | Organisms with alternative genetic codes resolve unassigned codons via mistranslation and ribosomal rescue |
| Q38582812 | Overcoming Challenges in Engineering the Genetic Code |
| Q92672749 | PERSIA for Direct Fluorescence Measurements of Transcription, Translation, and Enzyme Activity in Cell-Free Systems |
| Q33805165 | Patterns of sequencing coverage bias revealed by ultra-deep sequencing of vertebrate mitochondria |
| Q47263102 | Photo-Crosslinkable Unnatural Amino Acids Enable Facile Synthesis of Thermoresponsive Nano- to Microgels of Intrinsically Disordered Polypeptides. |
| Q41851361 | Piecing Together Cell-like Systems |
| Q49968034 | Playing with the molecules of life |
| Q41500111 | Precise manipulation of bacterial chromosomes by conjugative assembly genome engineering |
| Q39517573 | Predictive design of mRNA translation initiation region to control prokaryotic translation efficiency |
| Q21131128 | Preparing synthetic biology for the world |
| Q90278176 | Probing eukaryotic genome functions with synthetic chromosomes |
| Q34378484 | Probing the limits of genetic recoding in essential genes. |
| Q27693351 | Production of Fatty Acid-derived valuable chemicals in synthetic microbes |
| Q28263615 | Profile of George M. Church |
| Q92994051 | Programmed chromosome fission and fusion enable precise large-scale genome rearrangement and assembly |
| Q46081413 | Programming biological operating systems: genome design, assembly and activation |
| Q38194195 | Progress toward synthetic cells |
| Q57990689 | Prospective Applications of Synthetic Biology for Algal Bioproduct Optimization |
| Q27010058 | Protease-resistant peptide design-empowering nature's fragile warriors against HIV |
| Q38099295 | Protein engineering for metabolic engineering: current and next-generation tools |
| Q35238418 | Rapid editing and evolution of bacterial genomes using libraries of synthetic DNA. |
| Q35630262 | Rapid prototyping of microbial cell factories via genome-scale engineering. |
| Q35651308 | Rational design of an evolutionary precursor of glutaminyl-tRNA synthetase |
| Q37701249 | Rational optimization of tolC as a powerful dual selectable marker for genome engineering |
| Q43164101 | Reading and writing omes |
| Q51158886 | Reassigning Sense Codon AGA to Encode Noncanonical Amino Acids in Escherichia coli. |
| Q28081937 | Recent advances and versatility of MAGE towards industrial applications |
| Q36994135 | Recent advances in the discovery and development of marine microbial natural products. |
| Q41499658 | Recoded organisms engineered to depend on synthetic amino acids. |
| Q38084259 | Recombineering to homogeneity: extension of multiplex recombineering to large-scale genome editing |
| Q47142889 | Refactoring the Genetic Code for Increased Evolvability |
| Q34005133 | Reiterative Recombination for the in vivo assembly of libraries of multigene pathways. |
| Q36178052 | Release factor one is nonessential in Escherichia coli |
| Q28821229 | Repurposing the translation apparatus for synthetic biology |
| Q34142144 | Response and adaptation of Escherichia coli to suppression of the amber stop codon |
| Q28251152 | Revealing the amino acid composition of proteins within an expanded genetic code |
| Q38019718 | Rewiring translation - Genetic code expansion and its applications |
| Q39428992 | Rewriting the Genetic Code |
| Q27021207 | Rewriting the blueprint of life by synthetic genomics and genome engineering |
| Q36174731 | Robust orthogonal recombination system for versatile genomic elements rearrangement in yeast Saccharomyces cerevisiae. |
| Q28267113 | Robust production of recombinant phosphoproteins using cell-free protein synthesis |
| Q35841281 | Scalable amplification of strand subsets from chip-synthesized oligonucleotide libraries |
| Q38246195 | Selective chemical protein modification |
| Q44255571 | Selective pressure dominates the synonymous codon usage in parvoviridae |
| Q28305963 | Sense codon emancipation for proteome-wide incorporation of noncanonical amino acids: rare isoleucine codon AUA as a target for genetic code expansion |
| Q27671629 | Simplification of the genetic code: restricted diversity of genetically encoded amino acids |
| Q34044236 | Simultaneous non-contiguous deletions using large synthetic DNA and site-specific recombinases |
| Q38187359 | Small RNA regulators in bacteria: powerful tools for metabolic engineering and synthetic biology |
| Q40144153 | Specific modulation of protein activity by using a bioorthogonal reaction |
| Q21203748 | Stop codons in bacteria are not selectively equivalent |
| Q38321952 | Strategies for cloning and manipulating natural and synthetic chromosomes |
| Q38156504 | Strategies for the multiplex mapping of genes to traits |
| Q31064145 | Strategy for directing combinatorial genome engineering in Escherichia coli. |
| Q38044409 | Synthesis of post-translationally modified proteins |
| Q38784006 | Synthetic Biology-The Synthesis of Biology |
| Q47597781 | Synthetic Genomics: From DNA Synthesis to Genome Design. |
| Q54318151 | Synthetic RNA devices to expedite the evolution of metabolite-producing microbes. |
| Q38183353 | Synthetic biology of avermectin for production improvement and structure diversification. |
| Q28078320 | Synthetic biology to access and expand nature's chemical diversity |
| Q83912803 | Synthetic biology: Automated design of RNA devices |
| Q43839831 | Synthetic biology: Construction of a yeast chromosome |
| Q35907569 | Synthetic biology: advancing biological frontiers by building synthetic systems |
| Q38081904 | Synthetic biology: advancing the design of diverse genetic systems |
| Q34212825 | Synthetic biology: regulating industry uses of new biotechnologies |
| Q37176292 | Synthetic chromosome arms function in yeast and generate phenotypic diversity by design |
| Q38537984 | Synthetic chromosomes. |
| Q28729206 | Synthetic constructs in/for the environment: managing the interplay between natural and engineered Biology |
| Q38830330 | Synthetic genome engineering forging new frontiers for wine yeast |
| Q47407853 | Synthetic genome engineering gets infectious |
| Q41923159 | Synthetic genome recoding: new genetic codes for new features |
| Q40333580 | Systematic genetic dissection of PTS in Vibrio cholerae uncovers a novel glucose transporter and a limited role for PTS during infection of a mammalian host |
| Q42630018 | Targeted Large-Scale Deletion of Bacterial Genomes Using CRISPR-Nickases |
| Q47417619 | Targeted mutagenesis: A sniper-like diversity generator in microbial engineering |
| Q47249662 | The Future of Multiplexed Eukaryotic Genome Engineering. |
| Q24331642 | The Genome Project–Write |
| Q91713919 | The Role of Orthogonality in Genetic Code Expansion |
| Q39639221 | The Ssr protein (T1E_1405) from Pseudomonas putida DOT-T1E enables oligonucleotide-based recombineering in platform strain P. putida EM42. |
| Q28660797 | The biological microprocessor, or how to build a computer with biological parts |
| Q38215368 | The dawn of evolutionary genome engineering |
| Q42735640 | The genetic code. Rewritten, revised, repurposed |
| Q34497847 | The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coli |
| Q63874498 | Total synthesis of Escherichia coli with a recoded genome |
| Q39156083 | Total synthesis of a eukaryotic chromosome: Redesigning and SCRaMbLE-ing yeast. |
| Q33663564 | Total synthesis of a functional designer eukaryotic chromosome. |
| Q38669842 | Toward a genetic tool development pipeline for host-associated bacteria. |
| Q35819501 | Transient overexpression of DNA adenine methylase enables efficient and mobile genome engineering with reduced off-target effects |
| Q30396587 | Translation system engineering in Escherichia coli enhances non-canonical amino acid incorporation into proteins. |
| Q38208848 | Unnatural amino acid incorporation in E. coli: current and future applications in the design of therapeutic proteins |
| Q37683505 | Upgrading protein synthesis for synthetic biology. |
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