A standardized workflow for surveying recombinases expands bacterial genome-editing capabilities.

scientific article

A standardized workflow for surveying recombinases expands bacterial genome-editing capabilities. is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1111/1751-7915.12846
P932PMC publication ID5743808
P698PubMed publication ID29094478

P50authorÁkos NyergesQ42738850
Esteban Martínez-GarcíaQ73082120
P2093author name stringCsaba Pál
Tomás Aparicio
Víctor de Lorenzo
Deirdre E Ricaurte
P2860cites workRNA-guided editing of bacterial genomes using CRISPR-Cas systemsQ24630389
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR productsQ27860842
A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistanceQ28131606
Enzymatic assembly of DNA molecules up to several hundred kilobasesQ28241142
Precise manipulation of chromosomes in vivo enables genome-wide codon replacementQ28243372
Programming cells by multiplex genome engineering and accelerated evolutionQ28253066
DNA mismatch repairQ28256190
Genome-scale reconstruction and analysis of the Pseudomonas putida KT2440 metabolic network facilitates applications in biotechnologyQ28473829
An efficient recombination system for chromosome engineering in Escherichia coliQ29615038
A complementation analysis of the restriction and modification of DNA in Escherichia coliQ29615277
Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutantsQ33637017
Scarless and sequential gene modification in Pseudomonas using PCR product flanked by short homology regionsQ33648542
pBAM1: an all-synthetic genetic tool for analysis and construction of complex bacterial phenotypes.Q33826810
High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotidesQ33948878
Recombineering using RecTE from Pseudomonas syringaeQ34045936
Transposon-based and plasmid-based genetic tools for editing genomes of gram-negative bacteriaQ34074416
Enzymatic assembly of overlapping DNA fragmentsQ34186536
Pyrimidine metabolism in microorganismsQ34222939
Discontinuous DNA replicationQ34251800
Enhanced levels of lambda Red-mediated recombinants in mismatch repair mutantsQ34283357
Recombineering: A powerful tool for modification of bacteriophage genomesQ34293061
Improving lambda red genome engineering in Escherichia coli via rational removal of endogenous nucleasesQ34408253
Recombineering in Corynebacterium glutamicum combined with optical nanosensors: a general strategy for fast producer strain generationQ34695274
Rapid editing and evolution of bacterial genomes using libraries of synthetic DNA.Q35238418
Transient overexpression of DNA adenine methylase enables efficient and mobile genome engineering with reduced off-target effectsQ35819501
A highly precise and portable genome engineering method allows comparison of mutational effects across bacterial speciesQ35925574
High efficiency recombineering in lactic acid bacteriaQ36044894
Identification and analysis of recombineering functions from Gram-negative and Gram-positive bacteria and their phagesQ36446432
Industrial biotechnology of Pseudomonas putida and related speciesQ37986491
Biotechnological domestication of pseudomonads using synthetic biology.Q38204897
MutS interaction with mismatch and alkylated base containing DNA molecules detected by optical biosensorQ38351537
Mechanisms of solvent resistance mediated by interplay of cellular factors in Pseudomonas putidaQ38454138
Pseudomonas putida-a versatile host for the production of natural productsQ38534411
Tracing explosives in soil with transcriptional regulators of Pseudomonas putida evolved for responding to nitrotoluenes.Q39228979
Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coliQ39565526
The Ssr protein (T1E_1405) from Pseudomonas putida DOT-T1E enables oligonucleotide-based recombineering in platform strain P. putida EM42.Q39639221
Adaptation of the yeast URA3 selection system to gram-negative bacteria and generation of a {delta}betCDE Pseudomonas putida strainQ40986642
Rapid oligonucleotide-based recombineering of the chromosome of Salmonella enterica.Q41868385
Pseudomonas putida KT2440 Strain Metabolizes Glucose through a Cycle Formed by Enzymes of the Entner-Doudoroff, Embden-Meyerhof-Parnas, and Pentose Phosphate PathwaysQ41899338
Pseudomonas 2.0: genetic upgrading of P. putida KT2440 as an enhanced host for heterologous gene expression.Q41914986
CRISPR-Cas9-assisted recombineering in Lactobacillus reuteriQ41971637
Detection of novel recombinases in bacteriophage genomes unveils Rad52, Rad51 and Gp2.5 remote homologs.Q42060342
The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypesQ42137312
A high-efficiency recombineering system with PCR-based ssDNA in Bacillus subtilis mediated by the native phage recombinase GP35.Q42156455
Freeing Pseudomonas putida KT2440 of its proviral load strengthens endurance to environmental stressesQ42222540
Streptomycin-resistant and streptomycin-dependent mutants of the extreme thermophile Thermus thermophilusQ43028784
Molecular characterization of Rif(r) mutations in Pseudomonas aeruginosa and Pseudomonas putidaQ44892954
Efficient point mutagenesis in mycobacteria using single-stranded DNA recombineering: characterization of antimycobacterial drug targetsQ46790528
Pseudomonas putida KT2440 markerless gene deletion using a combination of λ Red recombineering and Cre/loxP site-specific recombinationQ48065683
A general system to integrate lacZ fusions into the chromosomes of gram-negative eubacteria: regulation of the Pm promoter of the TOL plasmid studied with all controlling elements in monocopy.Q54271765
Mutant sequences in the rpsL gene of Escherichia coli B/r: mechanistic implications for spontaneous and ultraviolet light mutagenesis.Q54274549
Engineering multiple genomic deletions in Gram-negative bacteria: analysis of the multi-resistant antibiotic profile of Pseudomonas putida KT2440Q56896083
Ribosomal proteins. 33. Location of amino-acid replacements in protein S12 isolated from Escherichia coli mutants resistant to streptomycinQ69201716
Streptomycin preferentially perturbs ribosomal proofreadingQ70409675
P433issue1
P921main subjectworkflowQ627335
P304page(s)176-188
P577publication date2017-11-02
P1433published inMicrobial BiotechnologyQ15766695
P1476titleA standardized workflow for surveying recombinases expands bacterial genome-editing capabilities
P478volume11

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cites work (P2860)
Q55348060Directed evolution of multiple genomic loci allows the prediction of antibiotic resistance.
Q61798003Evolutionary highways to persistent bacterial infection
Q90369297High-Efficiency Multi-site Genomic Editing of Pseudomonas putida through Thermoinducible ssDNA Recombineering
Q59360291Phage Therapy: What Have We Learned?
Q92999174Rapid Evolution of Reduced Susceptibility against a Balanced Dual-Targeting Antibiotic through Stepping-Stone Mutations
Q64103429Single-Stranded DNA-Binding Protein and Exogenous RecBCD Inhibitors Enhance Phage-Derived Homologous Recombination in Pseudomonas

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