Direct and Inverted Repeat stimulated excision (DIRex): Simple, single-step, and scar-free mutagenesis of bacterial genes

scientific article published on 30 August 2017

Direct and Inverted Repeat stimulated excision (DIRex): Simple, single-step, and scar-free mutagenesis of bacterial genes is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1371/JOURNAL.PONE.0184126
P932PMC publication ID5576700
P698PubMed publication ID28854250

P50authorJoakim NäsvallQ45792385
P2093author name stringJoakim Näsvall
P2860cites workPrecise and nearly-precise excision of the symmetrical inverted repeats of Tn 5; common features of recA-independent deletion events in Escherichia coliQ67234650
Transducing fragments in generalized transduction by phage P1. I. Molecular origin of the fragmentsQ72706367
Instability of palindromic DNA in Escherichia coliQ72911645
Excision of unstable artificial gene-specific inverted repeats mediates scar-free gene deletions in Escherichia coliQ86063539
Simple and highly efficient BAC recombineering using galK selectionQ24794075
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR productsQ27860842
Programming cells by multiplex genome engineering and accelerated evolutionQ28253066
An efficient recombination system for chromosome engineering in Escherichia coliQ29615038
Single-stranded heteroduplex intermediates in lambda Red homologous recombinationQ33644920
Rapid and highly efficient method for scarless mutagenesis within the Salmonella enterica chromosomeQ33802758
High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotidesQ33948878
Lambda red recombineering in Escherichia coli occurs through a fully single-stranded intermediate.Q34285766
Real-time evolution of new genes by innovation, amplification, and divergenceQ34307457
Positive and negative selection using the tetA-sacB cassette: recombineering and P1 transduction in Escherichia coliQ34383416
Engineered CRISPR-Cas9 nucleases with altered PAM specificitiesQ34481737
The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coliQ34497847
A highly precise and portable genome engineering method allows comparison of mutational effects across bacterial speciesQ35925574
Markerless Escherichia coli rrn Deletion Strains for Genetic Determination of Ribosomal Binding SitesQ36383088
Inverted DNA repeats: a source of eukaryotic genomic instabilityQ36698685
Compensating the Fitness Costs of Synonymous MutationsQ36904243
Mutagenic inverted repeat assisted genome engineering (MIRAGE).Q37041800
Improved seamless mutagenesis by recombineering using ccdB for counterselectionQ38257003
The enzymatic basis of processivity in lambda exonucleaseQ39744726
A new Vibrio fischeri lux gene precedes a bidirectional termination site for the lux operonQ39951996
Duplication-Insertion Recombineering: a fast and scar-free method for efficient transfer of multiple mutations in bacteria.Q40450325
Evidence for two mechanisms of palindrome-stimulated deletion in Escherichia coli: single-strand annealing and replication slipped mispairing.Q42132539
Phage P22-mutants with increased or decreased transduction abilitiesQ45252565
Activation of cryptic aminoglycoside resistance in Salmonella entericaQ45792333
Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coliQ45856655
P275copyright licenseCreative Commons Attribution 4.0 InternationalQ20007257
P6216copyright statuscopyrightedQ50423863
P433issue8
P407language of work or nameEnglishQ1860
P304page(s)e0184126
P577publication date2017-08-30
P1433published inPLOS OneQ564954
P1476titleDirect and Inverted Repeat stimulated excision (DIRex): Simple, single-step, and scar-free mutagenesis of bacterial genes
P478volume12

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cites work (P2860)
Q54202540Genetic Adaptation to Growth Under Laboratory Conditions in Escherichia coli and Salmonella enterica.
Q93095299Genetic Engineering by DNA Recombineering
Q89000209Structural mechanism of AadA, a dual-specificity aminoglycoside adenylyltransferase from Salmonella enterica

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