The molecular toolbox for chromosomal heterologous multiprotein expression in Escherichia coli

scientific article published on December 2012

The molecular toolbox for chromosomal heterologous multiprotein expression in Escherichia coli is …
instance of (P31):
review articleQ7318358
scholarly articleQ13442814

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P356DOI10.1042/BST20120143
P698PubMed publication ID23176458

P2093author name stringJohannes Gescher
Katrin Richter
P2860cites workSimple generation of site-directed point mutations in the Escherichia coli chromosome using Red(R)/ET(R) RecombinationQ38268186
Markerless gene replacement in Escherichia coli stimulated by a double-strand break in the chromosomeQ39729563
Purification and characterization of the in vitro activity of I-Sce I, a novel and highly specific endonuclease encoded by a group I intronQ40515080
A versatile element for gene addition in bacterial chromosomesQ40897496
DNA-dependent renaturation of an insoluble DNA binding protein. Identification of the RhaS binding site at rhaBAD.Q41429403
High-cell-density fermentation for production of L-N-carbamoylase using an expression system based on the Escherichia coli rhaBAD promoterQ43545708
Gene replacement without selection: regulated suppression of amber mutations in Escherichia coliQ44508201
Propionate-regulated high-yield protein production in Escherichia coliQ46540640
Dissimilatory iron reduction in Escherichia coli: identification of CymA of Shewanella oneidensis and NapC of E. coli as ferric reductasesQ46660315
New alkane-responsive expression vectors for Escherichia coli and pseudomonasQ48344962
Allelic exchange in Escherichia coli using the Bacillus subtilis sacB gene and a temperature-sensitive pSC101 repliconQ54696474
Construction and model-based analysis of a promoter library for E. coli: an indispensable tool for metabolic engineeringQ21256656
Engineering a reduced Escherichia coli genomeQ24682505
Simple and highly efficient BAC recombineering using galK selectionQ24794075
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR productsQ27860842
A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutantsQ29547327
An efficient recombination system for chromosome engineering in Escherichia coliQ29615038
Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinantQ29615039
A new logic for DNA engineering using recombination in Escherichia coliQ29615202
Conditional-replication, integration, excision, and retrieval plasmid-host systems for gene structure-function studies of bacteriaQ30731601
Tuning genetic control through promoter engineeringQ33222305
Fast, easy and efficient: site-specific insertion of transgenes into enterobacterial chromosomes using Tn7 without need for selection of the insertion eventQ33241518
Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combinationsQ33314657
Promoter knock-in: a novel rational method for the fine tuning of genesQ33545436
A propionate-inducible expression system for enteric bacteriaQ34143369
A novel phosphate-regulated expression vector in Escherichia coliQ34782435
Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systemsQ36514863
Cre/lox system and PCR-based genome engineering in Bacillus subtilisQ36897902
Heterologous protein production in Escherichia coli using the propionate-inducible pPro system by conventional and auto-induction methodsQ36960685
A new positive/negative selection scheme for precise BAC recombineeringQ37159406
P433issue6
P921main subjectEscherichia coliQ25419
P304page(s)1222-1226
P577publication date2012-12-01
P1433published inBiochemical Society TransactionsQ864226
P1476titleThe molecular toolbox for chromosomal heterologous multiprotein expression in Escherichia coli
P478volume40

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cites work (P2860)
Q35561910Advanced biotechnology: metabolically engineered cells for the bio-based production of chemicals and fuels, materials, and health-care products
Q42609940Designing overall stoichiometric conversions and intervening metabolic reactions
Q53352809Electron transport at the microbe-mineral interface: a synthesis of current research challenges.
Q28654732Metabolic Engineering of Escherichia coli for Production of Mixed-Acid Fermentation End Products

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