Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9.

scientific article published on December 2016

Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9. is …
instance of (P31):
scholarly articleQ13442814

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P6179Dimensions Publication ID1035773610
P356DOI10.1186/S12934-016-0605-5
P8608Fatcat IDrelease_bef6go75ozgqbgs5gsnzpwtfci
P932PMC publication ID5134288
P698PubMed publication ID27908280

P50authorChanghao BiQ88194344
P2093author name stringJing Li
Dongdong Zhao
Bin Xiong
Xueli Zhang
Lijun Ye
Hongnian Sun
Shenli Yuan
P2860cites workDe novo-engineered transcription activator-like effector (TALE) hybrid nuclease with novel DNA binding specificity creates double-strand breaksQ24624851
RNA-guided editing of bacterial genomes using CRISPR-Cas systemsQ24630389
Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas systemQ24669821
A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial ImmunityQ24669850
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR productsQ27860842
Programming cells by multiplex genome engineering and accelerated evolutionQ28253066
A TALE nuclease architecture for efficient genome editingQ28301656
CRISPR/Cas, the immune system of bacteria and archaeaQ29614423
An efficient recombination system for chromosome engineering in Escherichia coliQ29615038
Genome-scale CRISPR-Cas9 knockout screening in human cellsQ29616044
Transfection of Escherichia coli spheroplasts. V. Activity of recBC nuclease in rec+ and rec minus spheroplasts measured with different forms of bacteriophage DNAQ30450610
A one pot, one step, precision cloning method with high throughput capabilityQ33382728
An end-joining repair mechanism in Escherichia coliQ33719661
CRISPR/Cas9 advances engineering of microbial cell factories.Q34045724
Genome-scale promoter engineering by coselection MAGEQ34224602
Targeted chromosomal cleavage and mutagenesis in Drosophila using zinc-finger nucleasesQ34615432
j5 DNA assembly design automation softwareQ34708680
Genetic engineering using homologous recombinationQ34995474
Multigene editing in the Escherichia coli genome via the CRISPR-Cas9 systemQ35172182
CRMAGE: CRISPR Optimized MAGE RecombineeringQ36500383
Genome editing with RNA-guided Cas9 nuclease in zebrafish embryosQ36739960
Minimizing acetate formation in E. coli fermentations.Q36898780
Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonucleaseQ37271124
Consequences of Cas9 cleavage in the chromosome of Escherichia coliQ38883743
Coupling the CRISPR/Cas9 System with Lambda Red Recombineering Enables Simplified Chromosomal Gene Replacement in Escherichia coliQ41251787
PaR-PaR laboratory automation platformQ44484599
PR-PR: cross-platform laboratory automation systemQ46080807
Multiplex metabolic pathway engineering using CRISPR/Cas9 in Saccharomyces cerevisiae.Q46414542
An improved recombineering approach by adding RecA to lambda Red recombinationQ46871203
Combining metabolic engineering and metabolic evolution to develop nonrecombinant strains of Escherichia coli C that produce succinate and malateQ46922472
Metabolic engineering of Escherichia coli using CRISPR-Cas9 meditated genome editing.Q52423793
Engineering central metabolic modules of Escherichia coli for improving β-carotene production.Q54315207
DNA double-strand break repair by homologous recombinationQ64387340
P433issue1
P921main subjectEscherichia coliQ25419
CRISPRQ412563
Cas9Q16965677
CRISPR-Cas methodQ17310682
P304page(s)205
P577publication date2016-12-01
P1433published inMicrobial Cell FactoriesQ15766995
P1476titleDevelopment of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9.
P478volume15

Reverse relations

cites work (P2860)
Q57816801A Multiplex Genome Editing Method for Based on CRISPR-Cas12a
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Q64018790A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae
Q55023279CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing.
Q47104635CRISPR/Cas9-assisted gRNA-free one-step genome editing with no sequence limitations and improved targeting efficiency.
Q98469550Comprehensive study on Escherichia coli genomic expression: Does position really matter?
Q91660310Construction of Escherichia coli cell factories for crocin biosynthesis
Q96343134Construction of a carbon-conserving pathway for glycolate production by synergetic utilization of acetate and glucose in Escherichia coli
Q54202653Construction of an alternative glycerol-utilization pathway for improved β-carotene production in Escherichia coli.
Q47116177Development of a CRISPR/Cas9 genome editing toolbox for Corynebacterium glutamicum
Q47734697Development of an Efficient Genome Editing Tool in Bacillus licheniformis Using CRISPR-Cas9 Nickase
Q44887936Efficient gene editing in Corynebacterium glutamicum using the CRISPR/Cas9 system.
Q97528329Engineering Escherichia coli to improve tryptophan production via genetic manipulation of precursor and cofactor pathways
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Q92153252Experimental Challenges for Reduced Genomes: The Cell Model Escherichia coli
Q100454968Fast and antibiotic free genome integration into Escherichia coli chromosome
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Q47803170The CRISPR/Cas9-facilitated multiplex pathway optimization (CFPO) technique and its application to improve the Escherichia coli xylose utilization pathway

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