Structure-function analysis of MurJ reveals a solvent-exposed cavity containing residues essential for peptidoglycan biogenesis in Escherichia coli

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Structure-function analysis of MurJ reveals a solvent-exposed cavity containing residues essential for peptidoglycan biogenesis in Escherichia coli is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1128/JB.00731-13
P932PMC publication ID3807429
P698PubMed publication ID23935042

P50authorNatividad RuizQ56152943
Emily K ButlerQ58153089
P2093author name stringRebecca M Davis
Paul A Nicholson
Vase Bari
P2860cites workI-TASSER server for protein 3D structure predictionQ21284202
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Chemical genetic identification of peptidoglycan inhibitors potentiating carbapenem activity against methicillin-resistant Staphylococcus aureusQ38449021
Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and MuQ39605636
The NorM efflux pump of Neisseria gonorrhoeae and Neisseria meningitidis recognizes antimicrobial cationic compoundsQ39705786
Role of precursor translocation in coordination of murein and phospholipid synthesis in Escherichia coliQ39843562
Cluster of mrdA and mrdB genes responsible for the rod shape and mecillinam sensitivity of Escherichia coliQ40332495
Phosphatidylglycerol::prolipoprotein diacylglyceryl transferase (Lgt) of Escherichia coli has seven transmembrane segments, and its essential residues are embedded in the membraneQ41889476
Membrane topology and identification of critical amino acid residues in the Wzx O-antigen translocase from Escherichia coli O157:H4.Q42124251
Streptococcus pyogenes YtgP (Spy_0390) complements Escherichia coli strains depleted of the putative peptidoglycan flippase MurJ.Q42577649
The fourth transmembrane domain of the Helicobacter pylori Na+/H+ antiporter NhaA faces a water-filled channel required for ion transport.Q44985710
Peptidoglycan precursor pools associated with MraY and FtsW deficiencies or antibiotic treatmentsQ46649297
Control of cell shape and elongation by the rodA gene in Bacillus subtilisQ47986877
Enhanced recognition of protein transmembrane domains with prediction-based structural profilesQ48467132
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Ez, a Depth-dependent Potential for Assessing the Energies of Insertion of Amino Acid Side-chains into Membranes: Derivation and Applications to Determining the Orientation of Transmembrane and Interfacial HelicesQ57837597
Peptidoglycan synthetic activities in membranes of Escherichia coli caused by overproduction of penicillin-binding protein 2 and rodA proteinQ70137917
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A cationic lumen in the Wzx flippase mediates anionic O-antigen subunit translocation in Pseudomonas aeruginosa PAO1Q28492687
Membrane topology mapping of the O-antigen flippase (Wzx), polymerase (Wzy), and ligase (WaaL) from Pseudomonas aeruginosa PAO1 reveals novel domain architecturesQ28492786
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A comparative study of the reported performance of ab initio protein structure prediction algorithms.Q30366494
The bacterial cell envelopeQ33800394
Transmembrane protein topology mapping by the substituted cysteine accessibility method (SCAM(TM)): application to lipid-specific membrane protein topogenesisQ33922207
Identification of FtsW as a transporter of lipid-linked cell wall precursors across the membraneQ34169371
Identifying nonpolar transbilayer helices in amino acid sequences of membrane proteinsQ34181973
Wzx flippase-mediated membrane translocation of sugar polymer precursors in bacteriaQ34302243
The Escherichia coli cell division protein FtsW is required to recruit its cognate transpeptidase, FtsI (PBP3), to the division site.Q34305324
Membrane topology of the Rickettsia prowazekii ATP/ADP translocase revealed by novel dual pho-lac reportersQ34489200
A division inhibitor and a topological specificity factor coded for by the minicell locus determine proper placement of the division septum in E. coliQ34497481
Biosynthesis and assembly of capsular polysaccharides in Escherichia coliQ34535125
Involvement of an essential gene, mviN, in murein synthesis in Escherichia coliQ34809747
Bioinformatics identification of MurJ (MviN) as the peptidoglycan lipid II flippase in Escherichia coliQ34843809
Multidrug efflux transporters in the MATE family.Q34909208
Identification of FtsW and characterization of a new ftsW division mutant of Escherichia coliQ35980656
New mutations fts-36, lts-33, and ftsW clustered in the mra region of the Escherichia coli chromosome induce thermosensitive cell growth and divisionQ36182705
Structural similarity among Escherichia coli FtsW and RodA proteins and Bacillus subtilis SpoVE protein, which function in cell division, cell elongation, and spore formation, respectivelyQ36184478
Lipid intermediates in the biosynthesis of bacterial peptidoglycanQ36314747
Mapping of the mecillinam-resistant, round morphological mutants of Escherichia coliQ36417251
Characterization and Genetic Analysis of a Mutant of Escherichia coli K-12 with Rounded MorphologyQ36764600
Isolation of the Lipid Intermediate in Peptidoglycan Biosynthesis from Escherichia coliQ36836318
Murein (peptidoglycan) structure, architecture and biosynthesis in Escherichia coliQ36894116
Pivotal roles of the outer membrane polysaccharide export and polysaccharide copolymerase protein families in export of extracellular polysaccharides in gram-negative bacteriaQ37405778
Analysis of membrane protein topology using alkaline phosphatase and beta-galactosidase gene fusionsQ37479767
Flipping lipids: why an' what's the reason for?Q37581576
P433issue20
P407language of work or nameEnglishQ1860
P921main subjectEscherichia coliQ25419
P304page(s)4639-4649
P577publication date2013-08-09
P1433published inJournal of BacteriologyQ478419
P1476titleStructure-function analysis of MurJ reveals a solvent-exposed cavity containing residues essential for peptidoglycan biogenesis in Escherichia coli
P478volume195

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cites work (P2860)
Q39000262A Burkholderia cenocepacia MurJ (MviN) homolog is essential for cell wall peptidoglycan synthesis and bacterial viability
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Q33863113A genome-scale metabolic flux model of Escherichia coli K-12 derived from the EcoCyc database
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Q34175962Bacterial cell wall. MurJ is the flippase of lipid-linked precursors for peptidoglycan biogenesis.
Q34593511Charge requirements of lipid II flippase activity in Escherichia coli
Q27025371Core Steps of Membrane-Bound Peptidoglycan Biosynthesis: Recent Advances, Insight and Opportunities
Q42292711Crystal structure of the MOP flippase MurJ in an inward-facing conformation
Q90031296Detection of Transport Intermediates in the Peptidoglycan Flippase MurJ Identifies Residues Essential for Conformational Cycling
Q30869235Expediting topology data gathering for the TOPDB database
Q38905951Filling holes in peptidoglycan biogenesis of Escherichia coli
Q26770493Lipid Flippases for Bacterial Peptidoglycan Biosynthesis
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Q26775024LipidII: Just Another Brick in the Wall?
Q89122184Loss of specificity variants of WzxC suggest that substrate recognition is coupled with transporter opening in MOP-family flippases
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Q52646851Membrane Potential Is Required for MurJ Function.
Q46599265Membrane Translocation and Assembly of Sugar Polymer Precursors
Q35644600MurJ and a novel lipid II flippase are required for cell wall biogenesis in Bacillus subtilis.
Q36153263New Structural Templates for Clinically Validated and Novel Targets in Antimicrobial Drug Research and Development.
Q92867218Peptidoglycan
Q59351221Phage single-gene lysis: Finding the weak spot in the bacterial cell wall
Q57092020Structure and mutagenic analysis of the lipid II flippase MurJ from
Q38394431The Mycobacterial Cell Wall--Peptidoglycan and Arabinogalactan
Q36106787The O-Antigen Flippase Wzk Can Substitute for MurJ in Peptidoglycan Synthesis in Helicobacter pylori and Escherichia coli
Q93350317The bacterial lipid II flippase MurJ functions by an alternating-access mechanism
Q38872891Vibrio cholerae VciB Mediates Iron Reduction
Q64114313Visualizing conformation transitions of the Lipid II flippase MurJ

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