Construction of targeted single copy lac fusions using lambda Red and FLP-mediated site-specific recombination in bacteria

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Construction of targeted single copy lac fusions using lambda Red and FLP-mediated site-specific recombination in bacteria is …
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scholarly articleQ13442814

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P356DOI10.1016/S0378-1119(02)00551-6
P698PubMed publication ID12062810

P50authorJames M SlauchQ54142755
P2093author name stringCraig D Ellermeier
Anuradha Janakiraman
P2860cites workEfficient translation of the RpoS sigma factor in Salmonella typhimurium requires host factor I, an RNA-binding protein encoded by the hfq geneQ24643457
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR productsQ27860842
The FLP protein of the yeast 2-microns plasmid: expression of a eukaryotic genetic recombination system in Escherichia coliQ27932343
An efficient recombination system for chromosome engineering in Escherichia coliQ29615038
Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinantQ29615039
Improved single and multicopy lac-based cloning vectors for protein and operon fusionsQ29615296
Conditional-replication, integration, excision, and retrieval plasmid-host systems for gene structure-function studies of bacteriaQ30731601
Tissue-specific gene expression identifies a gene in the lysogenic phage Gifsy-1 that affects Salmonella enterica serovar typhimurium survival in Peyer's patches.Q33994441
Characterization of grvA, an antivirulence gene on the gifsy-2 phage in Salmonella enterica serovar typhimuriumQ33995341
Growth inhibition caused by overexpression of the structural gene for glutamate dehydrogenase (gdhA) from Klebsiella aerogenesQ33996112
cis-acting ompF mutations that result in OmpR-dependent constitutive expressionQ36148065
Convergently functional, Rho-independent terminator in Salmonella typhimuriumQ36362463
Virulent Salmonella typhimurium has two periplasmic Cu, Zn-superoxide dismutasesQ36398159
Genetic fusions as experimental toolsQ36439573
Convenient and reversible site-specific targeting of exogenous DNA into a bacterial chromosome by use of the FLP recombinase: the FLIRT systemQ39847367
Replication properties of plasmic R6K.Q40124150
A bacterial model system for chromosomal targetingQ40531072
The putative iron transport system SitABCD encoded on SPI1 is required for full virulence of Salmonella typhimurium.Q40625112
Role of EscF, a putative needle complex protein, in the type III protein translocation system of enteropathogenic Escherichia coliQ40770368
Tagging exported proteins using Escherichia coli alkaline phosphatase gene fusionsQ45039719
IVET and RIVET: use of gene fusions to identify bacterial virulence factors specifically induced in host tissues.Q50118831
Interaction of the recombination pathways of bacteriophage lambda and its host Escherichia coli K12: effects on exonuclease V activity.Q53679159
Interaction of the recombination pathways of bacteriophage lambda and host Escherichia coli: effects on lambda recombinationQ69212689
A practical guide to the construction and use of lac fusions in Escherichia coliQ73089703
Integration-proficient Pseudomonas aeruginosa vectors for isolation of single-copy chromosomal lacZ and lux gene fusionsQ73206292
Genetic system for reversible integration of DNA constructs and lacZ gene fusions into the Escherichia coli chromosomeQ73301843
P433issue1-2
P407language of work or nameEnglishQ1860
P304page(s)153-161
P577publication date2002-05-01
P1433published inGeneQ5531065
P1476titleConstruction of targeted single copy lac fusions using lambda Red and FLP-mediated site-specific recombination in bacteria
P478volume290

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cites work (P2860)
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Q24598644The small RNA SgrS controls sugar-phosphate accumulation by regulating multiple PTS genes
Q39203305The type VI secretion system encoded in Salmonella pathogenicity island 19 is required for Salmonella enterica serotype Gallinarum survival within infected macrophages.
Q47316351Transcriptional control of the antimicrobial peptide resistance ugtL gene by the Salmonella PhoP and SlyA regulatory proteins
Q40947162Transcriptional regulation of sitABCD of Salmonella enterica serovar Typhimurium by MntR and Fur
Q53361708Transketolase activity modulates glycerol-3-phosphate levels in Escherichia coli.
Q21563354Translation enhancing ACA motifs and their silencing by a bacterial small regulatory RNA
Q37265066Twin-arginine translocation system (tat) mutants of Salmonella are attenuated due to envelope defects, not respiratory defects
Q38323490Unexpected functional diversity among FadR fatty acid transcriptional regulatory proteins
Q42905654Unsaturated long chain free fatty acids are input signals of the Salmonella enterica PhoP/PhoQ regulatory system
Q41215879Virulence Gene Regulation by L-Arabinose in Salmonella enterica
Q50084995Virulence and drug resistance roles of multidrug efflux systems of Salmonella enterica serovar Typhimurium
Q39764932mgtA Expression is induced by rob overexpression and mediates a Salmonella enterica resistance phenotype

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