A method for generating precise gene deletions and insertions in Escherichia coli.

scientific article published on 8 January 2010

A method for generating precise gene deletions and insertions in Escherichia coli. is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1007/S11274-009-0305-Y
P698PubMed publication ID24026937
P5875ResearchGate publication ID256501589

P2093author name stringQi-Ming Zhou
Jun-Mei Zhou
Chuan-Peng Liu
Dong-Jie Fan
Jiang-Bi Xie
P2860cites workConstruction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collectionQ22122301
Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterizationQ24676777
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR productsQ27860842
An efficient recombination system for chromosome engineering in Escherichia coliQ29615038
A new logic for DNA engineering using recombination in Escherichia coliQ29615202
Combination of overlapping bacterial artificial chromosomes by a two-step recombinogenic engineering methodQ30783995
Structure of trigger factor binding domain in biologically homologous complex with eubacterial ribosome reveals its chaperone actionQ33913724
Epitope tagging of chromosomal genes in SalmonellaQ33953109
Gene replacement with linear DNA fragments in wild-type Escherichia coli: enhancement by Chi sitesQ33969790
A new method for generating point mutations in bacterial artificial chromosomes by homologous recombination in Escherichia coliQ34584758
Gene replacement with linear DNA in electroporated wild-type Escherichia coliQ34698796
Genetic engineering using homologous recombinationQ34995474
New method for generating deletions and gene replacements in Escherichia coliQ36181236
Highly efficient method for introducing successive multiple scarless gene deletions and markerless gene insertions into the Yersinia pestis chromosomeQ36746654
Modification of human beta-globin locus PAC clones by homologous recombination in Escherichia coliQ39543482
Rapid modification of bacterial artificial chromosomes by ET-recombination.Q39727582
Markerless gene replacement in Escherichia coli stimulated by a double-strand break in the chromosomeQ39729563
Trigger factor-assisted folding of bovine carbonic anhydrase II.Q44707229
A global regulatory role of gluconeogenic genes in Escherichia coli revealed by transcriptome network analysis.Q45944200
Structural rearrangements and the unfolding mechanism of a Trigger Factor mutant studied by multiple structural probesQ46070794
Antibiotic marker modifications of lambda Red and FLP helper plasmids, pKD46 and pCP20, for inactivation of chromosomal genes using PCR products in multidrug-resistant strains.Q46489005
Construction of targeted single copy lac fusions using lambda Red and FLP-mediated site-specific recombination in bacteriaQ48298401
Versatile suicide vectors which allow direct selection for gene replacement in gram-negative bacteria.Q50782746
A single-step transconjugation system for the introduction of unmarked deletions into Actinobacillus pleuropneumoniae serotype 7 using a sucrose sensitivity marker.Q54079534
Thermal unfolding of Escherichia coli trigger factor studied by ultra-sensitive differential scanning calorimetry.Q54421316
The C-terminal domain of Escherichia coli trigger factor represents the central module of its chaperone activity.Q54457243
Modular structure of the trigger factor required for high activity in protein folding.Q54558764
The amino-terminal 118 amino acids of Escherichia coli trigger factor constitute a domain that is necessary and sufficient for binding to ribosomes.Q54559605
An 11.8 kDa proteolytic fragment of the E. coli trigger factor represents the domain carrying the peptidyl-prolyl cis/trans isomerase activity.Q54590320
DNA cloning by homologous recombination in Escherichia coliQ73256761
P433issue7
P921main subjectEscherichia coliQ25419
P304page(s)1323-1329
P577publication date2010-01-08
P1433published inWorld Journal of Microbiology and BiotechnologyQ15749216
P1476titleA method for generating precise gene deletions and insertions in Escherichia coli.
P478volume26

Reverse relations

cites work (P2860)
Q37879979Gene replacement techniques for Escherichia coli genome modification.
Q50445944Markerless Deletion System for Escherichia coli Using Short Homologous Sequences and Positive-Negative Selectable Cassette
Q34487183The DNA-Binding Protein from Starved Cells (Dps) Utilizes Dual Functions To Defend Cells against Multiple Stresses.
Q35748055Use of transposase and ends of IS608 enables precise and scarless genome modification for modulating gene expression and metabolic engineering applications in Escherichia coli

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