Use of transposase and ends of IS608 enables precise and scarless genome modification for modulating gene expression and metabolic engineering applications in Escherichia coli

scientific article published on 17 August 2015

Use of transposase and ends of IS608 enables precise and scarless genome modification for modulating gene expression and metabolic engineering applications in Escherichia coli is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1002/BIOT.201500205
P698PubMed publication ID26282057

P2093author name stringHeidi Martini-Stoica
Chandresh Thakker
George N Bennett
Kevin Lin
P2860cites workConstruction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collectionQ22122301
Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterizationQ24676777
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One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR productsQ27860842
Recombineering: a homologous recombination-based method of genetic engineeringQ28307909
An efficient recombination system for chromosome engineering in Escherichia coliQ29615038
Conditional-replication, integration, excision, and retrieval plasmid-host systems for gene structure-function studies of bacteriaQ30731601
Scarless and sequential gene modification in Pseudomonas using PCR product flanked by short homology regionsQ33648542
High-efficiency scarless genetic modification in Escherichia coli by using lambda red recombination and I-SceI cleavageQ33743392
Sinorhizobium meliloti dctA mutants with partial ability to transport dicarboxylic acidsQ33758157
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Transposition of ISHp608, member of an unusual family of bacterial insertion sequencesQ34016167
Single-stranded DNA transposition is coupled to host replication.Q34057463
Transposable element ISHp608 of Helicobacter pylori: nonrandom geographic distribution, functional organization, and insertion specificityQ34305433
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Highly efficient method for introducing successive multiple scarless gene deletions and markerless gene insertions into the Yersinia pestis chromosomeQ36746654
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Simple generation of site-directed point mutations in the Escherichia coli chromosome using Red(R)/ET(R) RecombinationQ38268186
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Utilization of orotate as a pyrimidine source by Salmonella typhimurium and Escherichia coli requires the dicarboxylate transport protein encoded by dctA.Q39843737
Plasmid pCS1966, a new selection/counterselection tool for lactic acid bacterium strain construction based on the oroP gene, encoding an orotate transporter from Lactococcus lactis.Q41768365
The orotate transporter encoded by oroP from Lactococcus lactis is required for orotate utilization and has utility as a food-grade selectable marker.Q42636047
Application of lambda Red recombination system to Vibrio cholerae genetics: simple methods for inactivation and modification of chromosomal genesQ44431182
In vitro reconstitution of a single-stranded transposition mechanism of IS608.Q46749573
Site-specific integration of DNA into wild-type and mutant lox sites placed in the plant genomeQ49308730
A simple system for converting lacZ to gfp reporter fusions in diverse bacteria.Q50738288
Versatile suicide vectors which allow direct selection for gene replacement in gram-negative bacteria.Q50782746
Application of CRISPRi for prokaryotic metabolic engineering involving multiple genes, a case study: Controllable P(3HB-co-4HB) biosynthesis.Q52423508
A method for generating precise gene deletions and insertions in Escherichia coli.Q52643348
A single-step transconjugation system for the introduction of unmarked deletions into Actinobacillus pleuropneumoniae serotype 7 using a sucrose sensitivity marker.Q54079534
Allelic exchange in Escherichia coli using the Bacillus subtilis sacB gene and a temperature-sensitive pSC101 repliconQ54696474
P433issue1
P407language of work or nameEnglishQ1860
P921main subjectEscherichia coliQ25419
P304page(s)80-90
P577publication date2015-08-17
P1433published inBiotechnology JournalQ15716480
P1476titleUse of transposase and ends of IS608 enables precise and scarless genome modification for modulating gene expression and metabolic engineering applications in Escherichia coli
P478volume11

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Q35933619Using Group II Introns for Attenuating the In Vitro and In Vivo Expression of a Homing Endonucleasecites workP2860

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