Mutagenesis: A Method for Large-Scale Alteration of Viral Genomes

scientific article published on 20 February 2019

Mutagenesis: A Method for Large-Scale Alteration of Viral Genomes is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.3390/IJMS20040913
P932PMC publication ID6413233
P698PubMed publication ID30791544

P50authorJiajia TangQ91808357
Wolfram BruneQ40812930
P2093author name stringRenke Brixel
P2860cites workEn passant mutagenesis: a two step markerless red recombination system.Q50568023
A phase 1 study of 4 live, recombinant human cytomegalovirus Towne/Toledo chimeric vaccines.Q51918958
Improved Ribo-seq enables identification of cryptic translation events.Q52359216
Rapid identification of essential and nonessential herpesvirus genes by direct transposon mutagenesisQ57083076
Random transposon mutagenesis of large DNA molecules in Escherichia coliQ77426828
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Simple and highly efficient BAC recombineering using galK selectionQ24794075
Back to BAC: the use of infectious clone technologies for viral mutagenesisQ26859104
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR productsQ27860842
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A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNAQ29615197
Murine cytomegalovirus m41 open reading frame encodes a Golgi-localized antiapoptotic proteinQ31011536
Site-specific chromosomal integration of large synthetic constructsQ33760274
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Extensive genome-wide variability of human cytomegalovirus in congenitally infected infants.Q33916640
Forward with BACs: new tools for herpesvirus genomicsQ33927285
Functional map of human cytomegalovirus AD169 defined by global mutational analysisQ33972886
Reconstruction of the complete human cytomegalovirus genome in a BAC reveals RL13 to be a potent inhibitor of replication.Q34090187
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Construction of a self-excisable bacterial artificial chromosome containing the human cytomegalovirus genome and mutagenesis of the diploid TRL/IRL13 geneQ34971048
Human cytomegalovirus tegument protein pp71 (ppUL82) enhances the infectivity of viral DNA and accelerates the infectious cycleQ35886438
A cytomegalovirus-encoded inhibitor of apoptosis that suppresses caspase-8 activationQ36525307
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Use of bacterial artificial chromosomes in baculovirus research and recombinant protein expression: current trends and future perspectives.Q38114092
Genetic manipulation of poxviruses using bacterial artificial chromosome recombineeringQ39333011
The cytomegaloviral protein pUL138 acts as potentiator of tumor necrosis factor (TNF) receptor 1 surface density to enhance ULb'-encoded modulation of TNF-α signalingQ39462585
Construction and transposon mutagenesis in Escherichia coli of a full-length infectious clone of pseudorabies virus, an alphaherpesvirus.Q39595184
Cloning of the human cytomegalovirus (HCMV) genome as an infectious bacterial artificial chromosome in Escherichia coli: a new approach for construction of HCMV mutants.Q39595874
Human cytomegalovirus with IE-2 (UL122) deleted fails to express early lytic genesQ39602194
Rapid modification of bacterial artificial chromosomes by ET-recombination.Q39727582
Complete Genome Sequence of a Human Cytomegalovirus Strain AD169 Bacterial Artificial Chromosome CloneQ39756019
Human cytomegalovirus clinical isolates carry at least 19 genes not found in laboratory strains.Q39872953
Defined large-scale alterations of the human cytomegalovirus genome constructed by cotransfection of overlapping cosmidsQ39873911
Human cytomegalovirus UL131-128 genes are indispensable for virus growth in endothelial cells and virus transfer to leukocytesQ39960937
Cloning of the genomes of human cytomegalovirus strains Toledo, TownevarRIT3, and Towne long as BACs and site-directed mutagenesis using a PCR-based techniqueQ40659928
Selectable insertion and deletion mutagenesis of the human cytomegalovirus genome using the Escherichia coli guanosine phosphoribosyl transferase (gpt) geneQ41305546
Human cytomegalovirus UL131 open reading frame is required for epithelial cell tropismQ41839444
Assisted large fragment insertion by Red/ET-recombination (ALFIRE)--an alternative and enhanced method for large fragment recombineering.Q41956199
Cloning and sequencing of a highly productive, endotheliotropic virus strain derived from human cytomegalovirus TB40/E.Q45397798
Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coliQ45856655
Genetic content of wild-type human cytomegalovirusQ47709383
P275copyright licenseCreative Commons Attribution 4.0 InternationalQ20007257
P6216copyright statuscopyrightedQ50423863
P433issue4
P407language of work or nameEnglishQ1860
P921main subjecthuman betaherpesvirus 5Q6929
P304page(s)913
P577publication date2019-02-20
P1433published inInternational Journal of Molecular SciencesQ3153277
P1476titleCopy-Paste Mutagenesis: A Method for Large-Scale Alteration of Viral Genomes
P478volume20

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Q92709054Human cytomegalovirus glycoprotein B variants affect viral entry, cell fusion, and genome stabilitycites workP2860

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