Rapid and efficient construction of markerless deletions in the Escherichia coli genome

scientific article published on 21 June 2008

Rapid and efficient construction of markerless deletions in the Escherichia coli genome is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1093/NAR/GKN359
P932PMC publication ID2504295
P698PubMed publication ID18567910
P5875ResearchGate publication ID5288500

P2093author name stringMi Sun Kim
Sun Chang Kim
Byung Jo Yu
Bong Hyun Sung
Jun Hyoung Lee
Kui Hyeon Kang
P2860cites workIntrachromosomal homologous recombination in Arabidopsis thalianaQ64387384
Induction of homologous recombination in mammalian chromosomes by using the I-SceI system of Saccharomyces cerevisiaeQ24652685
Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterizationQ24676777
Engineering a reduced Escherichia coli genomeQ24682505
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR productsQ27860842
Environmentally controlled invasion of cancer cells by engineered bacteriaQ28285227
Arac/XylS family of transcriptional regulatorsQ28776772
An efficient recombination system for chromosome engineering in Escherichia coliQ29615038
A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNAQ29615197
A new logic for DNA engineering using recombination in Escherichia coliQ29615202
Scarless and site-directed mutagenesis in Salmonella enteritidis chromosomeQ33299277
Systematic mutagenesis of the Escherichia coli genomeQ33980052
Recombineering: a powerful new tool for mouse functional genomicsQ34389493
Emergent properties of reduced-genome Escherichia coli.Q34518764
Development of a biofilm production-deficient Escherichia coli strain as a host for biotechnological applicationsQ34649760
Genetic engineering using homologous recombinationQ34995474
Targeted mutagenesis by homologous recombination in D. melanogasterQ35778193
New method for generating deletions and gene replacements in Escherichia coliQ36181236
A regulatory cascade in the induction of rhaBAD.Q38314392
An improved method for deleting large regions of Escherichia coli K-12 chromosome using a combination of Cre/loxP and lambda Red.Q38341376
Use of new methods for construction of tightly regulated arabinose and rhamnose promoter fusions in studies of the Escherichia coli phosphate regulon.Q39564896
Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coliQ39565526
Markerless gene replacement in Escherichia coli stimulated by a double-strand break in the chromosomeQ39729563
Minimization of the Escherichia coli genome using a Tn5-targeted Cre/loxP excision systemQ40702240
Chromosomal deletion formation system based on Tn5 double transposition: use for making minimal genomes and essential gene analysisQ40949158
DNA-dependent renaturation of an insoluble DNA binding protein. Identification of the RhaS binding site at rhaBAD.Q41429403
Large-scale engineering of the Corynebacterium glutamicum genomeQ41954853
Genome engineering reveals large dispensable regions in Bacillus subtilis.Q44222483
Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coliQ45856655
Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome.Q47397444
In vivo recombineering of bacteriophage lambda by PCR fragments and single-strand oligonucleotides.Q50803970
Targeted modification of a human beta-globin locus BAC clone using GET Recombination and an I-Scei counterselection cassette.Q54523362
P433issue14
P921main subjectEscherichia coliQ25419
P304page(s)e84
P577publication date2008-06-21
P1433published inNucleic Acids ResearchQ135122
P1476titleRapid and efficient construction of markerless deletions in the Escherichia coli genome
P478volume36

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cites work (P2860)
Q57816801A Multiplex Genome Editing Method for Based on CRISPR-Cas12a
Q33639516A Novel and Efficient Method for Bacteria Genome Editing Employing both CRISPR/Cas9 and an Antibiotic Resistance Cassette
Q34518644A place for everything: chromosomal integration of large constructs
Q34538174A versatile and highly efficient method for scarless genome editing in Escherichia coli and Salmonella enterica.
Q98292617Advances on systems metabolic engineering of Bacillus subtilis as a chassis cell
Q35762450An Integrated System for Precise Genome Modification in Escherichia coli
Q46970389An electroporation-free method based on Red recombineering for markerless deletion and genomic replacement in the Escherichia coli DH1 genome
Q58004246Antibiotic Combinations That Enable One-Step, Targeted Mutagenesis of Chromosomal Genes
Q22065908Bacillus subtilis and Escherichia coli essential genes and minimal cell factories after one decade of genome engineering
Q36500383CRMAGE: CRISPR Optimized MAGE Recombineering
Q36245767Comparative genomics and experimental evolution of Escherichia coli BL21(DE3) strains reveal the landscape of toxicity escape from membrane protein overproduction
Q50057919Coupling the recombineering to Cre-lox system enables simplified large-scale genome deletion in Lactobacillus casei.
Q37836030Development of Genetic Tools for the Manipulation of the Planctomycetes
Q56896083Engineering multiple genomic deletions in Gram-negative bacteria: analysis of the multi-resistant antibiotic profile of Pseudomonas putida KT2440
Q53285476Enhancing recombinant protein production with an Escherichia coli host strain lacking insertion sequences.
Q34427520FRUIT, a scar-free system for targeted chromosomal mutagenesis, epitope tagging, and promoter replacement in Escherichia coli and Salmonella enterica.
Q37879979Gene replacement techniques for Escherichia coli genome modification.
Q37096587Genome engineering Escherichia coli for L-DOPA overproduction from glucose
Q38243092Genome engineering and gene expression control for bacterial strain development
Q33743392High-efficiency scarless genetic modification in Escherichia coli by using lambda red recombination and I-SceI cleavage
Q36127787Improved adhesive properties of recombinant bifidobacteria expressing the Bifidobacterium bifidum-specific lipoprotein BopA
Q37617370Inducible Prophage Mutant of Escherichia coli Can Lyse New Host and the Key Sites of Receptor Recognition Identification
Q36996756Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA sequences, including whole metabolic pathways, onto the Escherichia coli chromosome at well-characterised loci
Q35664673Layering genetic circuits to build a single cell, bacterial half adder
Q94528318Leveraging modern DNA assembly techniques for rapid, markerless genome modification
Q50445944Markerless Deletion System for Escherichia coli Using Short Homologous Sequences and Positive-Negative Selectable Cassette
Q35701287Meganucleases and other tools for targeted genome engineering: perspectives and challenges for gene therapy
Q38325411Molecular scissors and their application in genetically modified farm animals
Q35172182Multigene editing in the Escherichia coli genome via the CRISPR-Cas9 system
Q50443068Multiple-site genetic modifications in Escherichia coli using lambda-Red recombination and I-SceI cleavage
Q35541257On the road to synthetic life: the minimal cell and genome-scale engineering
Q35971568PCR-Based Seamless Genome Editing with High Efficiency and Fidelity in Escherichia coli
Q48065683Pseudomonas putida KT2440 markerless gene deletion using a combination of λ Red recombineering and Cre/loxP site-specific recombination
Q34201688Random and cyclical deletion of large DNA segments in the genome of Pseudomonas putida
Q33802758Rapid and highly efficient method for scarless mutagenesis within the Salmonella enterica chromosome
Q35630262Rapid prototyping of microbial cell factories via genome-scale engineering.
Q57068005Restriction-deficient mutants and marker-less genomic modification for metabolic engineering of the solvent producer
Q36284169Scarless deletion of up to seven methyl-accepting chemotaxis genes with an optimized method highlights key function of CheM in Salmonella Typhimurium.
Q34737391Selective advantage of resistant strains at trace levels of antibiotics: a simple and ultrasensitive color test for detection of antibiotics and genotoxic agents
Q33760274Site-specific chromosomal integration of large synthetic constructs
Q38321952Strategies for cloning and manipulating natural and synthetic chromosomes
Q40210049Tandem repeat coupled with endonuclease cleavage (TREC): a seamless modification tool for genome engineering in yeast.
Q34497847The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coli
Q43067525Tough nuts to crack: site-directed mutagenesis of bifidobacteria remains a challenge.

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