Large scale validation of an efficient CRISPR/Cas-based multi gene editing protocol in Escherichia coli

scientific article published on 24 April 2017

Large scale validation of an efficient CRISPR/Cas-based multi gene editing protocol in Escherichia coli is …
instance of (P31):
scholarly articleQ13442814

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P6179Dimensions Publication ID1084955187
P356DOI10.1186/S12934-017-0681-1
P932PMC publication ID5404680
P698PubMed publication ID28438207

P50authorEnrico KönigQ90264849
P2093author name stringGuido Grandi
Laura Fantappiè
Matteo Parri
Francesca Zerbini
Luisa Ganfini
Davide Fraccascia
Ilaria Zanella
Carmela Irene
Elena Caproni
Alberto Grandi
Luca F Frattini
Michele Tomasi
P2860cites workConstruction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collectionQ22122301
RNA-guided editing of bacterial genomes using CRISPR-Cas systemsQ24630389
Basic local alignment search toolQ25938991
Expanding the Biologist's Toolkit with CRISPR-Cas9Q26863745
Genome editing. The new frontier of genome engineering with CRISPR-Cas9Q28252298
Recombineering: a homologous recombination-based method of genetic engineeringQ28307909
An efficient recombination system for chromosome engineering in Escherichia coliQ29615038
A new logic for DNA engineering using recombination in Escherichia coliQ29615202
High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotidesQ33948878
Antibody-mediated immunity induced by engineered Escherichia coli OMVs carrying heterologous antigens in their lumenQ34037511
CRISPR/Cas9 advances engineering of microbial cell factories.Q34045724
The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coliQ34497847
Genetic engineering using homologous recombinationQ34995474
Recombineering with overlapping single-stranded DNA oligonucleotides: testing a recombination intermediateQ35163354
Multigene editing in the Escherichia coli genome via the CRISPR-Cas9 systemQ35172182
Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteriaQ36279805
Cloning structural gene sacB, which codes for exoenzyme levansucrase of Bacillus subtilis: expression of the gene in Escherichia coliQ36413372
Bacterial DNA repair by non-homologous end joiningQ36972411
Exploiting CRISPR-Cas immune systems for genome editing in bacteriaQ38656652
Next Generation Prokaryotic Engineering: The CRISPR-Cas ToolkitQ38761455
Exploring the potential of genome editing CRISPR-Cas9 technologyQ38796245
CRISPR technologies for bacterial systems: Current achievements and future directionsQ38810498
Consequences of Cas9 cleavage in the chromosome of Escherichia coliQ38883743
Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coliQ39565526
Rapid modification of bacterial artificial chromosomes by ET-recombination.Q39727582
Mycobacterium tuberculosis and Mycobacterium marinum non-homologous end-joining proteins can function together to join DNA ends in Escherichia coliQ40545064
Coupling the CRISPR/Cas9 System with Lambda Red Recombineering Enables Simplified Chromosomal Gene Replacement in Escherichia coliQ41251787
A rapid and simple method for inactivating chromosomal genes in YersiniaQ41465604
An efficient stress-free strategy to displace stable bacterial plasmidsQ43112051
Rapid and Efficient One-Step Metabolic Pathway Integration in E. coli.Q45973216
The Polymerase Incomplete Primer Extension (PIPE) method applied to high-throughput cloning and site-directed mutagenesisQ46261504
RecG helicase promotes DNA double-strand break repairQ47864494
A set of recombineering plasmids for gram-negative bacteriaQ50080127
[Genetic analysis of sacB, the structural gene of a secreted enzyme, levansucrase of Bacillus subtilis Marburg]Q70445937
PCR-mediated gene replacement in Escherichia coliQ73682242
P433issue1
P921main subjectCRISPRQ412563
Escherichia coliQ25419
P304page(s)68
P577publication date2017-04-24
P1433published inMicrobial Cell FactoriesQ15766995
P1476titleLarge scale validation of an efficient CRISPR/Cas-based multi gene editing protocol in Escherichia coli
P478volume16

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cites work (P2860)
Q92755065CRISPR-Cas9-mediated genomic multiloci integration in Pichia pastoris
Q90350496CRISPR/Cas9 recombineering-mediated deep mutational scanning of essential genes in Escherichia coli
Q97646074Development and Application of CRISPR/Cas in Microbial Biotechnology
Q90402062Development and characterization of a CRISPR/Cas9n-based multiplex genome editing system for Bacillus subtilis
Q64056385Editing the microbiome the CRISPR way
Q44887936Efficient gene editing in Corynebacterium glutamicum using the CRISPR/Cas9 system.
Q55147278Engineering CRISPR interference system in Klebsiella pneumoniae for attenuating lactic acid synthesis.
Q47215173Fundamental CRISPR-Cas9 tools and current applications in microbial systems
Q93052007Genome-driven cell engineering review: in vivo and in silico metabolic and genome engineering
Q89458516Improved sgRNA design in bacteria via genome-wide activity profiling
Q64055986Optimizing a CRISPR-Cpf1-based genome engineering system for Corynebacterium glutamicum
Q59360291Phage Therapy: What Have We Learned?

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