Efficient and seamless DNA recombineering using a thymidylate synthase A selection system in Escherichia coli

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Efficient and seamless DNA recombineering using a thymidylate synthase A selection system in Escherichia coli is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1093/NAR/GNI059
P3181OpenCitations bibliographic resource ID5035910
P932PMC publication ID1072810
P698PubMed publication ID15800210
P5875ResearchGate publication ID7936231

P50authorJian-Dong HuangQ70624280
P2093author name stringDanny Chan
Hsiang-Fu Kung
Marie C M Lin
Vivian C W Ng
Queenie N Y Wong
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Highly efficient modification of bacterial artificial chromosomes (BACs) using novel shuttle vectors containing the R6Kgamma origin of replicationQ31120342
Point mutation of bacterial artificial chromosomes by ET recombinationQ33757023
An exonuclease induced by bacteriophage lambda. II. Nature of the enzymatic reactionQ34243330
Recombineering: a powerful new tool for mouse functional genomicsQ34389493
A new method for generating point mutations in bacterial artificial chromosomes by homologous recombination in Escherichia coliQ34584758
Genetic engineering using homologous recombinationQ34995474
RecE/RecT and Redalpha/Redbeta initiate double-stranded break repair by specifically interacting with their respective partnersQ35200055
A simple two-step, 'hit and fix' method to generate subtle mutations in BACs using short denatured PCR fragmentsQ35222962
Primary structure of the Escherichia coli thyA gene and its thymidylate synthase productQ37345416
Evidence for the double-strand break repair model of bacteriophage lambda recombination.Q37735129
The beta protein of phage lambda promotes strand exchange.Q38338035
Modification of human beta-globin locus PAC clones by homologous recombination in Escherichia coliQ39543482
Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coliQ39565526
A general method for the consecutive integration of single copies of a heterologous gene at multiple locations in the Bacillus subtilis chromosome by replacement recombinationQ39798877
Site-directed mutagenesis of human type X collagen. Expression of alpha1(X) NC1, NC2, and helical mutations in vitro and in transfected cellsQ41192699
Intron-exon structure, alternative use of promoter and expression of the mouse collagen X gene, Col10a-1.Q42621740
Double-chain-cut sites are recombination hotspots in the Red pathway of phage lambdaQ43730426
A single-step transconjugation system for the introduction of unmarked deletions into Actinobacillus pleuropneumoniae serotype 7 using a sucrose sensitivity marker.Q54079534
Targeted modification of a human beta-globin locus BAC clone using GET Recombination and an I-Scei counterselection cassette.Q54523362
Identification of yrrU as the Methylthioadenosine Nucleosidase Gene in Bacillus subtilisQ57177001
Aberrant signal peptide cleavage of collagen X in Schmid metaphyseal chondrodysplasia. Implications for the molecular basis of the diseaseQ73294190
Rapid engineering of bacterial artificial chromosomes using oligonucleotidesQ73339514
PCR-mediated gene replacement in Escherichia coliQ73682242
Insertion of common mutations into the human beta-globin locus using GET Recombination and an EcoRI endonuclease counterselection cassetteQ78779904
P433issue6
P407language of work or nameEnglishQ1860
P921main subjectEscherichia coliQ25419
P304page(s)e59
P577publication date2005-03-30
P1433published inNucleic Acids ResearchQ135122
P1476titleEfficient and seamless DNA recombineering using a thymidylate synthase A selection system in Escherichia coli
P478volume33

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cites work (P2860)
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