Techniques: Recombinogenic engineering--new options for cloning and manipulating DNA.

scientific article

Techniques: Recombinogenic engineering--new options for cloning and manipulating DNA. is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1016/S0968-0004(00)01757-6
P698PubMed publication ID11343926

P2093author name stringZhang Y
Muyrers JP
Stewart AF
P2860cites workCloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vectorQ24563904
Cloning and mutagenesis of a herpesvirus genome as an infectious bacterial artificial chromosomeQ24645354
A simplified system for generating recombinant adenovirusesQ24682328
Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoterQ27860697
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR productsQ27860842
Yeast transformation: a model system for the study of recombinationQ28264331
An efficient recombination system for chromosome engineering in Escherichia coliQ29615038
A new logic for DNA engineering using recombination in Escherichia coliQ29615202
Cloning high molecular weight DNA fragments by the bacteriophage P1 systemQ30988603
Point mutation of bacterial artificial chromosomes by ET recombinationQ33757023
Forward with BACs: new tools for herpesvirus genomicsQ33927285
Construction of large DNA segments in Escherichia coliQ34502907
Modification of bacterial artificial chromosomes through Chi-stimulated homologous recombination and its application in zebrafish transgenesisQ36064760
New method for generating deletions and gene replacements in Escherichia coliQ36181236
Selective isolation of cosmid clones by homologous recombination in Escherichia coliQ36264772
A new bacteriophage P1-derived vector for the propagation of large human DNA fragmentsQ36745476
Modification of human beta-globin locus PAC clones by homologous recombination in Escherichia coliQ39543482
Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coliQ39565526
Insertion of disease-causing mutations in BACs by homologous recombination in Escherichia coliQ39584225
Cloning and mutagenesis of the murine gammaherpesvirus 68 genome as an infectious bacterial artificial chromosomeQ39592358
Rapid construction in yeast of complex targeting vectors for gene manipulation in the mouseQ39718164
Rapid modification of bacterial artificial chromosomes by ET-recombination.Q39727582
Simplified generation of targeting constructs using ET recombinationQ39728772
Lambda Gam protein inhibits the helicase and chi-stimulated recombination activities of Escherichia coli RecBCD enzymeQ39943196
Homologous pairing proteins encoded by the Escherichia coli recE and recT genesQ40734537
Direct cloning of genomic DNA by recombinogenic targeting method using a yeast-bacterial shuttle vector, pClasperQ40910099
Reduction of liver Fas expression by an antisense oligonucleotide protects mice from fulminant hepatitisQ43505905
Efficient and precise engineering of a 200 kb beta-globin human/bacterial artificial chromosome in E. coli DH10B using an inducible homologous recombination systemQ45859696
BAC trimming: minimizing clone overlapsQ47878838
Homologous recombination based modification in Esherichia coli and germline transmission in transgenic mice of a bacterial artificial chromsomeQ48611307
Recombinogenic targeting: a new approach to genomic analysis--a reviewQ52181381
Manipulating large genomic clones via in vivo recombination in bacteriaQ53919706
In vivo intermolecular recombination in Escherichia coli: application to plasmid constructionsQ54590198
Rapid engineering of bacterial artificial chromosomes using oligonucleotidesQ73339514
PCR-mediated gene replacement in Escherichia coliQ73682242
P433issue5
P1104number of pages7
P304page(s)325-331
P577publication date2001-05-01
P13046publication type of scholarly workreview articleQ7318358
P1433published inTrends in Biochemical SciencesQ1565711
P1476titleTechniques: Recombinogenic engineering--new options for cloning and manipulating DNA.
P478volume26

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cites work (P2860)
Q50717337A Gateway-based platform for multigene plant transformation
Q29615157A highly efficient recombineering-based method for generating conditional knockout mutations
Q37159406A new positive/negative selection scheme for precise BAC recombineering
Q41582297A new recombineering system for Photorhabdus and Xenorhabdus
Q34206930A novel PCR-based method for high throughput prokaryotic expression of antimicrobial peptide genes
Q50714542A recombineering pipeline for functional genomics applied to Caenorhabditis elegans
Q47867233A reliable lacZ expression reporter cassette for multipurpose, knockout-first alleles
Q46352630A scalable platform to identify fungal secondary metabolites and their gene clusters
Q37125259An Improved Method for oriT -Directed Cloning and Functionalization of Large Bacterial Genomic Regions
Q46871203An improved recombineering approach by adding RecA to lambda Red recombination
Q41590605Analysis and validation of traits associated with a single nucleotide polymorphism Gly364Ser in catestatin using humanized chromogranin A mouse models
Q41956199Assisted large fragment insertion by Red/ET-recombination (ALFIRE)--an alternative and enhanced method for large fragment recombineering.
Q45156523BAC‐recombineering for studying plant gene regulation: developmental control and cellular localization of SnRK1 kinase subunits
Q28474337BRED: a simple and powerful tool for constructing mutant and recombinant bacteriophage genomes
Q26859104Back to BAC: the use of infectious clone technologies for viral mutagenesis
Q24318410Building a spindle of the correct length in human cells requires the interaction between TPX2 and Aurora A
Q42523611Caenorhabditis elegans reporter fusion genes generated by seamless modification of large genomic DNA clones
Q33431522Chapter 7. Cloning and analysis of natural product pathways
Q34518585Characterization of Kiss1 neurons using transgenic mouse models
Q37229297Characterization of a novel ghrelin cell reporter mouse
Q30783995Combination of overlapping bacterial artificial chromosomes by a two-step recombinogenic engineering method
Q30487705Comparative profiling identifies C13orf3 as a component of the Ska complex required for mammalian cell division
Q48148067Controlled transgene dosage and PAC-mediated transgenesis in mice using a chromosomal vector.
Q27655489Crystal Structure of E. coli RecE Protein Reveals a Toroidal Tetramer for Processing Double-Stranded DNA Breaks
Q64231038Crystal structure of the Redβ C-terminal domain in complex with λ Exonuclease reveals an unexpected homology with λ Orf and an interaction with Escherichia coli single stranded DNA binding protein
Q27670686Crystal structures of λ exonuclease in complex with DNA suggest an electrostatic ratchet mechanism for processivity
Q39318649DNA annealing by Redβ is insufficient for homologous recombination and the additional requirements involve intra- and inter-molecular interactions.
Q36678592DNA signatures for detecting genetic engineering in bacteria.
Q44317432Deletion of Aspergillus nidulans aroC using a novel blaster module that combines ET cloning and marker rescue.
Q43674980Delivery of multiple transgenes to plant cells by an improved version of MultiRound Gateway technology
Q47443295Desacyl Ghrelin Decreases Anxiety-like Behavior in Male Mice
Q36073802Development of a bacterial artificial chromosome (BAC) recombineering procedure using galK-untranslated region (UTR) for the mutation of diploid genes
Q38604510Direct Capture Technologies for Genomics-Guided Discovery of Natural Products
Q52582173Discovery of recombinases enables genome mining of cryptic biosynthetic gene clusters in Burkholderiales species.
Q38311760Domain structure and DNA binding regions of beta protein from bacteriophage lambda
Q34508569Dual gene expression cassette vectors with antibiotic selection markers for engineering in Saccharomyces cerevisiae
Q35022531Efficient linking and transfer of multiple genes by a multigene assembly and transformation vector system
Q48147201Efficient strategy for introducing large and multiple changes in plasmid DNA.
Q42086545Efficient transfer of two large secondary metabolite pathway gene clusters into heterologous hosts by transposition
Q54528956Engineering the mouse genome with bacterial artificial chromosomes to create multipurpose alleles
Q54204592Escherichia coli as a host for metabolic engineering
Q37253822Examining a DNA Replication Requirement for Bacteriophage λ Red- and Rac Prophage RecET-Promoted Recombination in Escherichia coli
Q39378698Expression and characterization of codon-optimized Crimean-Congo hemorrhagic fever virus Gn glycoprotein in insect cells
Q25257854Expression and function profiling of orphan nuclear receptors using bacterial artificial chromosome (BAC) transgenesis
Q37869837From RNAi screens to molecular function in embryonic stem cells.
Q28594134Functional analysis of bacterial artificial chromosomes in mammalian cells: mouse Cdc6 is associated with the mitotic spindle apparatus
Q36931020Functional and genetic analysis of haplotypic sequence variation at the nicastrin genomic locus
Q50447234Functional characterization of different ORFs including luciferase-like monooxygenase genes from the mensacarcin gene cluster
Q36499874Gel electrophoresis in a polyvinylalcohol coated fused silica capillary for purity assessment of modified and secondary-structured oligo- and polyribonucleotides
Q41883375Generating in vivo cloning vectors for parallel cloning of large gene clusters by homologous recombination.
Q35920812Generation and Characterisation of a Pax8-CreERT2 Transgenic Line and a Slc22a6-CreERT2 Knock-In Line for Inducible and Specific Genetic Manipulation of Renal Tubular Epithelial Cells
Q48723209Generation and characterization of Neurod1-CreER(T2) mouse lines for the study of embryonic and adult neurogenesis
Q36217348Genetic characterization of the wboA gene from the predominant biovars of Brucella isolates in Iran
Q42684856Genetic modifications of mouse proopiomelanocortin peptide processing
Q28505051Globosides but not isoglobosides can impact the development of invariant NKT cells and their interaction with dendritic cells
Q50421814Heterologous expression and antitumor activity analysis of syringolin from Pseudomonas syringae pv. syringae B728a.
Q28202486High-throughput engineering of the mouse genome coupled with high-resolution expression analysis
Q27334278High-throughput in vivo analysis of gene expression in Caenorhabditis elegans
Q33531815Human cytomegaloviruses expressing yellow fluorescent fusion proteins--characterization and use in antiviral screening
Q36443015Hypertension, kidney, and transgenics: a fresh perspective
Q51784202IL7-hCD25 and IL7-Cre BAC transgenic mouse lines: new tools for analysis of IL-7 expressing cells
Q42005367Improved insecticidal toxicity by fusing Cry1Ac of Bacillus thuringiensis with Av3 of Anemonia viridis
Q38257003Improved seamless mutagenesis by recombineering using ccdB for counterselection
Q35757633In vivo construction of transgenes in Drosophila
Q52658206Interrogation of Benzomalvin Biosynthesis Using Fungal Artificial Chromosomes with Metabolomic Scoring (FAC-MS): Discovery of a Benzodiazepine Synthase Activity.
Q35123497Invited Review: Functional genomics in the mouse: powerful techniques for unraveling the basis of human development and disease
Q42746364Ligation independent cloning vectors for expression of SUMO fusions
Q36371228Major histocompatibility complex class I allele-specific cooperative and competitive interactions between immune evasion proteins of cytomegalovirus
Q92565699Manipulating the Mouse Genome Using Recombineering
Q84713043Mapping cell fate and function using recombinase-based intersectional strategies
Q24536127Mice lacking ghrelin receptors resist the development of diet-induced obesity
Q38353278Microinjection of Cre recombinase protein into zygotes enables specific deletion of two eukaryotic selection cassettes and enhances the expression of a DsRed2 reporter gene in Ccr2/Ccr5 double-deficient mice
Q51812826Mini-λ: a tractable system for chromosome and BAC engineering
Q34228002Mining the metabiome: identifying novel natural products from microbial communities
Q28296492Molecular neuroanatomy's "Three Gs": a primer
Q35132317Neuroanatomical and functional characterization of CRF neurons of the amygdala using a novel transgenic mouse model.
Q35749606Normal development and function of invariant natural killer T cells in mice with isoglobotrihexosylceramide (iGb3) deficiency
Q35024820One-pot DNA construction for synthetic biology: the Modular Overlap-Directed Assembly with Linkers (MODAL) strategy.
Q24798568Phage annealing proteins promote oligonucleotide-directed mutagenesis in Escherichia coli and mouse ES cells
Q33713988Physiological transgene regulation and functional complementation of a neurological disease gene deficiency in neurons
Q36434513Production of recombineering substrates with standard-size PCR primers
Q41982319Prospects for the use of artificial chromosomes and minichromosome-like episomes in gene therapy.
Q36515078Prostaglandin-dependent modulation of dopaminergic neurotransmission elicits inflammation-induced aversion in mice
Q42547895Proton- and ammonium-sensing by histaminergic neurons controlling wakefulness
Q33861783Quantitative proteomics combined with BAC TransgeneOmics reveals in vivo protein interactions
Q40988187REPLACR-mutagenesis, a one-step method for site-directed mutagenesis by recombineering
Q30856358RNA interference rescue by bacterial artificial chromosome transgenesis in mammalian tissue culture cells
Q33236095RecET driven chromosomal gene targeting to generate a RecA deficient Escherichia coli strain for Cre mediated production of minicircle DNA.
Q38119844Recent advances in the heterologous expression of microbial natural product biosynthetic pathways
Q37792222Recombineering BAC transgenes for protein tagging
Q43053415Recombineering with tolC as a selectable/counter-selectable marker: remodeling the rRNA operons of Escherichia coli
Q51551994Recombineering, transfection, Western, IP and ChIP methods for protein tagging via gene targeting or BAC transgenesis
Q34389493Recombineering: a powerful new tool for mouse functional genomics
Q24805845Recombining overlapping BACs into a single larger BAC
Q33648542Scarless and sequential gene modification in Pseudomonas using PCR product flanked by short homology regions
Q36076149Seamless cloning and gene fusion.
Q31049620Shuffled antibody libraries created by in vivo homologous recombination and yeast surface display
Q33644920Single-stranded heteroduplex intermediates in lambda Red homologous recombination
Q28066046Strategies used for genetically modifying bacterial genome: site-directed mutagenesis, gene inactivation, and gene over-expression
Q37385825Studying human telomerase gene transcription by a chromatinized reporter generated by recombinase-mediated targeting of a bacterial artificial chromosome
Q40182069Synergistic effects of deleting multiple nonessential elements in nonreplicative HSV-1 BAC genomic vectors play a critical role in their viability.
Q50769767Targeted modification of the complete chicken lysozyme gene by poxvirus-mediated recombination
Q33748014Targeting Vector Construction Through Recombineering
Q28593412The HSV-1 exonuclease, UL12, stimulates recombination by a single strand annealing mechanism
Q35188415The enzymology of combinatorial biosynthesis
Q42024421The expression of a recombinant cry1Ac gene with subtilisin-like protease CDEP2 gene in acrystalliferous Bacillus thuringiensis by Red/ET homologous recombination
Q40104715The role of ghrelin-responsive mediobasal hypothalamic neurons in mediating feeding responses to fasting
Q37077050Transposition-based method for the rapid generation of gene-targeting vectors to produce Cre/Flp-modifiable conditional knock-out mice
Q45957295[Cloning, expression and comparative analysis of peroxiredoxine 6 from different species]
Q36757820lambda-Red genetic engineering in Salmonella enterica serovar Typhimurium
Q37217540β1-Adrenergic receptor deficiency in ghrelin-expressing cells causes hypoglycemia in susceptible individuals

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