Multiple-step chromosomal integration of divided segments from a large DNA fragment via CRISPR/Cas9 in Escherichia coli

scientific article published on 23 November 2018

Multiple-step chromosomal integration of divided segments from a large DNA fragment via CRISPR/Cas9 in Escherichia coli is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1007/S10295-018-2114-5
P698PubMed publication ID30470963

P2093author name stringNing Chen
Yanjun Li
Xiaoguang Fan
Guoliang Li
Qian Ma
Chenglin Zhang
Qingyang Xu
Xixian Xie
Yakun Han
Fangqing Yan
Heyun Wu
P2860cites workA PCR-free cloning method for the targeted φ80 Int-mediated integration of any long DNA fragment, bracketed with meganuclease recognition sites, into the Escherichia coli chromosome.Q50513623
Metabolic engineering of Escherichia coli using CRISPR-Cas9 meditated genome editing.Q52423793
Establishment of a novel gene expression method, BICES (biomass-inducible chromosome-based expression system), and its application to the production of 2,3-butanediol and acetoin.Q53477414
Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivoQ58771152
Genome-scale engineering for systems and synthetic biologyQ22122302
CRISPR RNA maturation by trans-encoded small RNA and host factor RNase IIIQ24628207
RNA-guided editing of bacterial genomes using CRISPR-Cas systemsQ24630389
A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial ImmunityQ24669850
The future of metabolic engineering and synthetic biology: towards a systematic practiceQ27024888
Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuelsQ28263425
The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNAQ28297640
Recombineering: a homologous recombination-based method of genetic engineeringQ28307909
An efficient recombination system for chromosome engineering in Escherichia coliQ29615038
Site-specific chromosomal integration of large synthetic constructsQ33760274
Multiplexed genome engineering and genotyping methods applications for synthetic biology and metabolic engineeringQ33907809
Multigene editing in the Escherichia coli genome via the CRISPR-Cas9 systemQ35172182
Improvement of uridine production of Bacillus subtilis by atmospheric and room temperature plasma mutagenesis and high-throughput screeningQ36363033
Factors affecting plasmid production in Escherichia coli from a resource allocation standpointQ37242559
Genome-scale genetic engineering in Escherichia coliQ38102505
Genome engineering and gene expression control for bacterial strain developmentQ38243092
Coupling the CRISPR/Cas9 System with Lambda Red Recombineering Enables Simplified Chromosomal Gene Replacement in Escherichia coliQ41251787
Stabilized gene duplication enables long-term selection-free heterologous pathway expression.Q45919322
One-step cloning and chromosomal integration of DNA.Q46000081
Harnessing novel chromosomal integration loci to utilize an organosolv-derived hemicellulose fraction for isobutanol production with engineered Corynebacterium glutamicumQ47096818
P433issue1
P407language of work or nameEnglishQ1860
P921main subjectEscherichia coliQ25419
CRISPRQ412563
Cas9Q16965677
CRISPR-Cas methodQ17310682
P304page(s)81-90
P577publication date2019-01-01
P1433published inJournal of Industrial Microbiology & BiotechnologyQ1709847
P1476titleMultiple-step chromosomal integration of divided segments from a large DNA fragment via CRISPR/Cas9 in Escherichia coli
P478volume46

Reverse relations

Q97646074Development and Application of CRISPR/Cas in Microbial Biotechnologycites workP2860

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