Evaluation of digital PCR for absolute RNA quantification

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Evaluation of digital PCR for absolute RNA quantification is …
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scholarly articleQ13442814

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P819ADS bibcode2013PLoSO...875296S
P356DOI10.1371/JOURNAL.PONE.0075296
P932PMC publication ID3779174
P698PubMed publication ID24073259
P5875ResearchGate publication ID261991396

P50authorJim HuggettQ42409978
P2093author name stringRebecca Sanders
Deborah J Mason
Carole A Foy
P2860cites workReverse transcriptase can inhibit PCR and stimulate primer-dimer formationQ73034523
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The type of reverse transcriptase affects the sensitivity of some reverse transcription PCR methodsQ45300662
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Reverse transcriptase. The use of cloned Moloney murine leukemia virus reverse transcriptase to synthesize DNA from RNA.Q45760193
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Digital PCRQ24685020
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PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiencyQ24814623
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Comparison of different standards for real-time PCR-based absolute quantificationQ28271608
Detection limits of several commercial reverse transcriptase enzymes: impact on the low- and high-abundance transcript levels assessed by quantitative RT-PCRQ33303493
Mathematical analysis of copy number variation in a DNA sample using digital PCR on a nanofluidic deviceQ33358153
Digital PCR provides sensitive and absolute calibration for high throughput sequencingQ33419711
Reverse transcriptase (RT) inhibition of PCR at low concentrations of template and its implications for quantitative RT-PCR.Q33706250
Applicability of RNA standards for evaluating RT-qPCR assays and platformsQ33824130
Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems.Q34146463
Application of next generation qPCR and sequencing platforms to mRNA biomarker analysisQ34355270
The power of real-time PCR.Q34444172
Methods for applying accurate digital PCR analysis on low copy DNA samplesQ34614779
Pitfalls of quantitative real-time reverse-transcription polymerase chain reactionQ35872589
Digital PCR for the molecular detection of fetal chromosomal aneuploidyQ35916813
Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variationQ36008206
The development of modern metrology and its role todayQ36251265
Single molecule detection in nanofluidic digital array enables accurate measurement of DNA copy numberQ39877742
Comparison of Methods for Accurate Quantification of DNA Mass Concentration with Traceability to the International System of UnitsQ39944850
Evaluation of digital PCR for absolute DNA quantificationQ39976950
Digital PCR provides absolute quantitation of viral load for an occult RNA virusQ40002107
P275copyright licenseCreative Commons Attribution 4.0 InternationalQ20007257
P6216copyright statuscopyrightedQ50423863
P433issue9
P407language of work or nameEnglishQ1860
P304page(s)e75296
P577publication date2013-09-20
P1433published inPLOS OneQ564954
P1476titleEvaluation of digital PCR for absolute RNA quantification
P478volume8

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