The Future of Digital Polymerase Chain Reaction in Virology

scientific article published on 28 June 2016

The Future of Digital Polymerase Chain Reaction in Virology is …
instance of (P31):
scholarly articleQ13442814
review articleQ7318358

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P6179Dimensions Publication ID1008030161
P356DOI10.1007/S40291-016-0224-1
P8608Fatcat IDrelease_luwymdkudvfstgivtpgqtzxb7m
P698PubMed publication ID27351921

P50authorLinos VandekerckhoveQ41564602
Ward De SpiegelaereQ47494712
Matthijs VynckQ47557042
Olivier ThasQ55221789
P2093author name stringWim Trypsteen
P2860cites workImpact of variance components on reliability of absolute quantification using digital PCRQ21284300
Evaluation of a droplet digital polymerase chain reaction format for DNA copy number quantificationQ24606577
Digital PCRQ24685020
PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiencyQ24814623
Are three generations of quantitative molecular methods sufficient in medical virology? Brief reviewQ26781267
A new mathematical model for relative quantification in real-time RT-PCRQ27860781
Simultaneous amplification and detection of specific DNA sequencesQ28185655
The MIQE guidelines: minimum information for publication of quantitative real-time PCR experimentsQ28236560
Quantitative and sensitive detection of rare mutations using droplet-based microfluidicsQ28238121
High-throughput droplet digital PCR system for absolute quantitation of DNA copy numberQ28251729
Specific enzymatic amplification of DNA in vitro: the polymerase chain reactionQ29618837
Optimization of Droplet Digital PCR from RNA and DNA extracts with direct comparison to RT-qPCR: Clinical implications for quantification of Oseltamivir-resistant subpopulations.Q30378470
Low copy target detection by Droplet Digital PCR through application of a novel open access bioinformatic pipeline, 'definetherain'Q30486801
Elimination of amplification artifacts in real-time reverse transcription PCR using laser capture microdissected samplesQ30845398
Mathematical analysis of copy number variation in a DNA sample using digital PCR on a nanofluidic deviceQ33358153
Digital droplet PCR (ddPCR) for the precise quantification of human T-lymphotropic virus 1 proviral loads in peripheral blood and cerebrospinal fluid of HAM/TSP patients and identification of viral mutationsQ33858337
Absolute quantification by droplet digital PCR versus analog real-time PCR.Q33988019
Clinical utility of droplet digital PCR for human cytomegalovirusQ34058634
Quantitative analysis of viral load per haploid genome revealed the different biological features of Merkel cell polyomavirus infection in skin tumorQ34328459
Low-level detection and quantitation of cellular HIV-1 DNA and 2-LTR circles using droplet digital PCR.Q40062663
Standard reference material 2366 for measurement of human cytomegalovirus DNA.Q40085854
The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR ExperimentsQ40103939
Tolerance of droplet-digital PCR vs real-time quantitative PCR to inhibitory substancesQ40134987
Comparison of droplet digital PCR and seminested real-time PCR for quantification of cell-associated HIV-1 RNA.Q40166933
Comparative study of sensitivity, linearity, and resistance to inhibition of digital and nondigital polymerase chain reaction and loop mediated isothermal amplification assays for quantification of human cytomegalovirus.Q40181094
High-throughput and sensitive next-generation droplet digital PCR assay for the quantitation of the hepatitis C virus mutation at core amino acid 70.Q40207280
Quantitative detection of human enteric adenoviruses in river water by microfluidic digital polymerase chain reaction.Q40212987
Optimising droplet digital PCR analysis approaches for detection and quantification of bacteria: a case study of fire blight and potato brown rot.Q40218944
Comparison of next-generation droplet digital PCR (ddPCR) with quantitative PCR (qPCR) for enumeration of Cryptosporidium oocysts in faecal samplesQ40222808
A multiplexed droplet digital PCR assay performs better than qPCR on inhibition prone samplesQ40226170
Accurate quantification of episomal HIV-1 two-long terminal repeat circles by use of optimized DNA isolation and droplet digital PCR.Q40243427
Highly reproducible absolute quantification of Mycobacterium tuberculosis complex by digital PCR.Q40254479
A comparative study of digital RT-PCR and RT-qPCR for quantification of Hepatitis A virus and Norovirus in lettuce and water samples.Q40260306
ddpcRquant: threshold determination for single channel droplet digital PCR experimentsQ40284073
Rational design of digital assays.Q40297815
HIV-1 RNA and HIV-1 DNA persistence during suppressive ART with PI-based or nevirapine-based regimensQ40309618
Comparison of digital PCR platforms and semi-nested qPCR as a tool to determine the size of the HIV reservoirQ40311527
Statistical Models for the Analysis and Design of Digital Polymerase Chain Reaction (dPCR) ExperimentsQ40319721
Robust regression methods for real-time polymerase chain reactionQ41090459
A short history of the polymerase chain reaction.Q53374368
Comparison of four digital PCR platforms for accurate quantification of DNA copy number of a certified plasmid DNA reference materialQ34490796
Highly precise measurement of HIV DNA by droplet digital PCR.Q34661612
Quantitative analysis of food and feed samples with droplet digital PCR.Q34712633
Evaluation of digital PCR for absolute RNA quantificationQ35000858
Reverse transcriptase droplet digital PCR shows high resilience to PCR inhibitors from plant, soil and water samples.Q35016174
DNA copy number concentration measured by digital and droplet digital quantitative PCR using certified reference materialsQ35107472
Comparative evaluation of three commercial quantitative cytomegalovirus standards by use of digital and real-time PCR.Q35455475
Theoretical design and analysis of multivolume digital assays with wide dynamic range validated experimentally with microfluidic digital PCRQ35551811
Standardization of Nucleic Acid Tests for Clinical Measurements of Bacteria and Viruses.Q35757751
Development of Lentivirus-Based Reference Materials for Ebola Virus Nucleic Acid Amplification Technology-Based AssaysQ35839873
Identification of chromosomally integrated human herpesvirus 6 by droplet digital PCR.Q35879617
A Novel High-Throughput Method for Molecular Detection of Human Pathogenic Viruses Using a Nanofluidic Real-Time PCR System.Q35908557
Integrated and Total HIV-1 DNA Predict Ex Vivo Viral OutgrowthQ35944804
Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variationQ36008206
Measuring Digital PCR Quality: Performance Parameters and Their OptimizationQ36009664
MethyLight droplet digital PCR for detection and absolute quantification of infrequently methylated allelesQ36214749
Digital PCR for direct quantification of viruses without DNA extractionQ36446826
Viral diagnostics in the era of digital polymerase chain reactionQ36460329
Detection of Rare Drug Resistance Mutations by Digital PCR in a Human Influenza A Virus Model System and Clinical Samples.Q36521356
Comparison of droplet digital PCR to real-time PCR for quantitative detection of cytomegalovirusQ36559052
Human immunodeficiency virus-infected individuals contain provirus in small numbers of peripheral mononuclear cells and at low copy numbersQ36801268
Quantitative Assessment of Commutability for Clinical Viral Load Testing Using a Digital PCR-Based Reference StandardQ36933103
Flexible analysis of digital PCR experiments using generalized linear mixed modelsQ37172643
One-step RT-droplet digital PCR: a breakthrough in the quantification of waterborne RNA virusesQ37480155
Antiretrovirals for HIV prevention: when should they be recommended?Q38195335
The potential advantages of digital PCR for clinical virology diagnosticsQ38203913
Considerations for digital PCR as an accurate molecular diagnostic tool.Q38262012
A sensitive and accurate quantification method for the detection of hepatitis B virus covalently closed circular DNA by the application of a droplet digital polymerase chain reaction amplification systemQ38851736
Digital PCR: A brief historyQ39030238
Single molecule detection in nanofluidic digital array enables accurate measurement of DNA copy numberQ39877742
Quantifying EGFR alterations in the lung cancer genome with nanofluidic digital PCR arraysQ39922627
Digital PCR provides absolute quantitation of viral load for an occult RNA virusQ40002107
Digital PCR for noninvasive detection of aneuploidy: power analysis equations for feasibilityQ40032786
Molecular quantification of environmental DNA using microfluidics and digital PCR.Q40052289
P433issue5
P921main subjectvirologyQ7215
P304page(s)437-447
P577publication date2016-06-28
P1433published inMolecular Diagnosis and TherapyQ15758832
P1476titleThe Future of Digital Polymerase Chain Reaction in Virology
P478volume20

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cites work (P2860)
Q39180702Applications of Digital PCR for Clinical Microbiology
Q93074012Clinical correlation of influenza and respiratory syncytial virus load measured by digital PCR
Q94564607Development of a Digital RT-PCR Method for Absolute Quantification of Bluetongue Virus in Field Samples
Q58128506Development of sensitive ddPCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant forms
Q37286538Digital PCR Panel for Sensitive Hematopoietic Chimerism Quantification after Allogeneic Stem Cell Transplantation
Q47548213Digital PCR as a tool to measure HIV persistence
Q64945089Droplet Digital PCR for Estimating Absolute Abundances of Widespread Pelagibacter Viruses.
Q55265466Evaluation of bisulfite kits for DNA methylation profiling in terms of DNA fragmentation and DNA recovery using digital PCR.
Q40494985Quality control of digital PCR assays and platforms.
Q38290061Superiority of Digital Reverse Transcription-PCR (RT-PCR) over Real-Time RT-PCR for Quantitation of Highly Divergent Human Rhinoviruses
Q38664177The Role of BEAMing and Digital PCR for Multiplexed Analysis in Molecular Oncology in the Era of Next-Generation Sequencing

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