A continuous-exchange cell-free protein synthesis system based on extracts from cultured insect cells

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A continuous-exchange cell-free protein synthesis system based on extracts from cultured insect cells is …
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scholarly articleQ13442814

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P819ADS bibcode2014PLoSO...996635S
P356DOI10.1371/JOURNAL.PONE.0096635
P932PMC publication ID4013096
P698PubMed publication ID24804975
P5875ResearchGate publication ID262145990

P2093author name stringRita Sachse
Stefan Kubick
Doreen A Wüstenhagen
Robert B Quast
Corina Schulze
Marlitt Stech
P2860cites workCaspase-mediated cleavage of eukaryotic translation initiation factor subunit 2alphaQ24531676
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Protein prenylation in an insect cell-free protein synthesis system and identification of products by mass spectrometryQ33285213
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The effect of transcription and translation initiation frequencies on the stochastic fluctuations in prokaryotic gene expression.Q52072001
Production of functional antibody fragments in a vesicle-based eukaryotic cell-free translation system.Q52743832
A systematic approach to increase the efficiency of membrane protein production in cell-free expression systems.Q54342190
Cell-free production of functional antibody fragments.Q54399789
Insertion of membrane proteins into discoidal membranes using a cell-free protein expression approach.Q54420971
Reconstitution of the Cpx signaling system from cell-free synthesized proteins.Q54664740
Production of tPA in recombinant CHO cells under oxygen-limited conditionsQ58089304
Protein Incorporation in Giant Lipid Vesicles under Physiological ConditionsQ63888777
Gene expression in a cell-free system on the preparative scaleQ69565533
Refolding of bovine pancreatic trypsin inhibitor via non-native disulphide intermediatesQ71697197
A semicontinuous prokaryotic coupled transcription/translation system using a dialysis membraneQ71701856
Identification of caspase 3-mediated cleavage and functional alteration of eukaryotic initiation factor 2alpha in apoptosisQ73586104
The genetics of disulfide bond metabolismQ77936221
Functional cell-free synthesis of a seven helix membrane protein: in situ insertion of bacteriorhodopsin into liposomesQ80515596
Cell-free protein production and labeling protocol for NMR-based structural proteomicsQ81554430
Proteolysis of human eukaryotic translation initiation factor eIF4GII, but not eIF4GI, coincides with the shutoff of host protein synthesis after poliovirus infectionQ36307005
Preparative scale cell-free expression systems: new tools for the large scale preparation of integral membrane proteins for functional and structural studiesQ36580045
Immune response to a differentiation antigen induced by altered antigen: a study of tumor rejection and autoimmunityQ37053497
Cell-free protein expression based on extracts from CHO cells.Q39098150
Fed-batch CHO cell t-PA production and feed glutamine replacement to reduce ammonia production.Q39249582
Expression of active human tissue-type plasminogen activator in Escherichia coli.Q39479612
A study of proteases and protease-inhibitor complexes in biological fluidsQ39497760
Functional evaluation of candidate ice structuring proteins using cell-free expression systems.Q39511330
Eukaryotic translation initiation factor 4G is targeted for proteolytic cleavage by caspase 3 during inhibition of translation in apoptotic cellsQ39577003
The tissue-type plasminogen activator storyQ39824852
Induction of apoptosis in SF21 cell line by conditioned medium of the entomopathogenic fungus, Nomuraea rileyi, through Sf-caspase-1 signaling pathwayQ39994175
Cleavage of polypeptide chain initiation factor eIF4GI during apoptosis in lymphoma cells: characterisation of an internal fragment generated by caspase-3-mediated cleavageQ40869105
Variants of human tissue-type plasminogen activator that lack specific structural domains of the heavy chain.Q41106800
Prolonging cell-free protein synthesis with a novel ATP regeneration systemQ41704507
Cell-free complements in vivo expression of the E. coli membrane proteomeQ41865425
Cell-free synthesis of functional and endotoxin-free antibody Fab fragments by translocation into microsomesQ42011645
Cell-free synthesis and characterization of a novel cytotoxic pierisin-like protein from the cabbage butterfly Pieris rapaeQ42018939
Establishment and characterization of cell-free translation/glycosylation in insect cell (Spodoptera frugiperda 21) extract prepared with high pressure treatmentQ42053958
Cell-free synthesis of enzymically active tissue-type plasminogen activator. Protein folding determines the extent of N-linked glycosylationQ42800638
Practical cell-free protein synthesis system using purified wheat embryosQ43172288
The history of Z-VAD-FMK, a tool for understanding the significance of caspase inhibitionQ43693526
Expression of Fab fragment of catalytic antibody 6D9 in an Escherichia coli in vitro coupled transcription/translation systemQ43953826
Secretion in yeast: reconstitution of the translocation and glycosylation of alpha-factor and invertase in a homologous cell-free systemQ44301810
Cell-free systems: functional modules for synthetic and chemical biologyQ44316747
Efficient synthesis of a disulfide‐containing protein through a batch cell‐free system from wheat germQ44658329
High level cell-free expression and specific labeling of integral membrane proteinsQ44731897
Enhancing multiple disulfide bonded protein folding in a cell-free systemQ44820944
Expression of functional Candida antarctica lipase B in a cell-free protein synthesis system derived from Escherichia coliQ46084505
Improvements in the cell-free production of functional antibodies using cell extract from protease-deficient Escherichia coli mutantQ46760825
Optimized in vitro and in vivo expression of proteorhodopsin: a seven-transmembrane proton pumpQ46811125
Factors affecting endotoxin removal from recombinant therapeutic proteins by anion exchange chromatographyQ47245382
Expression and activity of the cytolethal distending toxin of Helicobacter hepaticusQ47601702
P275copyright licenseCreative Commons Attribution 4.0 InternationalQ20007257
P6216copyright statuscopyrightedQ50423863
P433issue5
P407language of work or nameEnglishQ1860
P921main subjectinsectQ1390
P304page(s)e96635
P577publication date2014-05-07
P1433published inPLOS OneQ564954
P1476titleA continuous-exchange cell-free protein synthesis system based on extracts from cultured insect cells
P478volume9

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