Quantitative immunofluorescence assay to measure the variation in protein levels at centrosomes

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Quantitative immunofluorescence assay to measure the variation in protein levels at centrosomes is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.3791/52030
P932PMC publication ID4396923
P698PubMed publication ID25548932
P5875ResearchGate publication ID270290977

P2093author name stringHarold A Fisk
Shubhra Majumder
P2860cites workCentriole assembly and the role of Mps1: defensible or dispensable?Q21203546
Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methodsQ24296940
Poc1A and Poc1B act together in human cells to ensure centriole integrityQ24299615
Proteomic characterization of the human centrosome by protein correlation profilingQ24301045
Mapping the NPHP-JBTS-MKS protein network reveals ciliopathy disease genes and pathwaysQ24302034
Centrin-2 is required for centriole duplication in mammalian cellsQ24304368
The SCF-FBXW5 E3-ubiquitin ligase is regulated by PLK4 and targets HsSAS-6 to control centrosome duplicationQ24310354
Human microcephaly protein CEP135 binds to hSAS-6 and CPAP, and is required for centriole assemblyQ24337030
Cep97 and CP110 suppress a cilia assembly programQ24338361
Plk1-dependent recruitment of gamma-tubulin complexes to mitotic centrosomes involves multiple PCM componentsQ24338881
Regulated HsSAS-6 levels ensure formation of a single procentriole per centriole during the centrosome duplication cycleQ24647101
Caenorhabditis elegans centriolar protein SAS-6 forms a spiral that is consistent with imparting a ninefold symmetryQ27678771
Disruption of a ciliary B9 protein complex causes Meckel syndromeQ28114969
A mechanism linking extra centrosomes to chromosomal instabilityQ29617923
Polo kinase and separase regulate the mitotic licensing of centriole duplication in human cells.Q30490330
Control of centrin stability by Aurora A.Q33952284
Plk4 trans-autophosphorylation regulates centriole number by controlling betaTrCP-mediated degradationQ34118510
Building the CentrioleQ34205722
VDAC3 regulates centriole assembly by targeting Mps1 to centrosomesQ34296794
Antizyme restrains centrosome amplification by regulating the accumulation of Mps1 at centrosomesQ34313419
VDAC3 and Mps1 negatively regulate ciliogenesisQ34326587
Mps1 phosphorylation sites regulate the function of centrin 2 in centriole assemblyQ34408480
Physical and functional interaction between mortalin and Mps1 kinaseQ34638240
Hedgehog signal transduction by Smoothened: pharmacologic evidence for a 2-step activation processQ34945251
PLK1 phosphorylation of pericentrin initiates centrosome maturation at the onset of mitosisQ35636562
Preventing the degradation of mps1 at centrosomes is sufficient to cause centrosome reduplication in human cellsQ36095688
Visualization of Mad2 dynamics at kinetochores, along spindle fibers, and at spindle poles in living cellsQ36293815
The autoregulated instability of Polo-like kinase 4 limits centrosome duplication to once per cell cycleQ36496562
A chemical method for fast and sensitive detection of DNA synthesis in vivoQ36497275
Mitosis-specific anchoring of gamma tubulin complexes by pericentrin controls spindle organization and mitotic entryQ37031680
Molecular architecture of the centriole proteome: the conserved WD40 domain protein POC1 is required for centriole duplication and length controlQ37097360
Regulating the transition from centriole to basal bodyQ37871223
CEP proteins: the knights of centrosome dynastyQ38086108
Quantitative analysis of cytoskeletal organization by digital fluorescent microscopyQ39652855
Proteomic analysis of isolated chlamydomonas centrioles reveals orthologs of ciliary-disease genesQ39720851
Accuracy and precision in quantitative fluorescence microscopyQ39891401
Parkin is recruited to the centrosome in response to inhibition of proteasomesQ42798009
DSas-6 and Ana2 coassemble into tubules to promote centriole duplication and engagementQ42871178
Cullin 1 functions as a centrosomal suppressor of centriole multiplication by regulating polo-like kinase 4 protein levelsQ43068860
Centrosome cohesion is regulated by a balance of kinase and phosphatase activitiesQ43798628
Detection of S-phase cell cycle progression using 5-ethynyl-2'-deoxyuridine incorporation with click chemistry, an alternative to using 5-bromo-2'-deoxyuridine antibodiesQ46556507
Detailed analysis of cell cycle kinetics upon proteasome inhibitionQ73508420
SAS-6 is a cartwheel protein that establishes the 9-fold symmetry of the centrioleQ80205133
P433issue94
P921main subjectimmunofluorescence microscopyQ592324
P577publication date2014-12-20
P1433published inJournal of Visualized ExperimentsQ954500
P1476titleQuantitative immunofluorescence assay to measure the variation in protein levels at centrosomes

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cites work (P2860)
Q36224555Centrin 3 is an inhibitor of centrosomal Mps1 and antagonizes centrin 2 function
Q52881129Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins.
Q37640216Let-7e modulates the inflammatory response in vascular endothelial cells through ceRNA crosstalk
Q36105568Non-Overlapping Distributions and Functions of the VDAC Family in Ciliogenesis

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