Signalling to actin assembly via the WASP (Wiskott-Aldrich syndrome protein)-family proteins and the Arp2/3 complex

scientific article published on May 2004

Signalling to actin assembly via the WASP (Wiskott-Aldrich syndrome protein)-family proteins and the Arp2/3 complex is …
instance of (P31):
scholarly articleQ13442814
review articleQ7318358

External links are
P356DOI10.1042/BJ20040176
P932PMC publication ID1224166
P698PubMed publication ID15040784

P50authorLaura MacheskyQ21264247
P2093author name stringThomas H Millard
Stewart J Sharp
P2860cites workA Rap guanine nucleotide exchange factor enriched highly in the basal gangliaQ22004019
WAVE, a novel WASP-family protein involved in actin reorganization induced by RacQ22008483
Identification of two human WAVE/SCAR homologues as general actin regulatory molecules which associate with the Arp2/3 complexQ22010165
Autoinhibition and activation mechanisms of the Wiskott-Aldrich syndrome proteinQ22253357
Scar/WAVE-1, a Wiskott-Aldrich syndrome protein, assembles an actin-associated multi-kinase scaffoldQ24290178
IRSp53 is an essential intermediate between Rac and WAVE in the regulation of membrane rufflingQ24290667
Activation of Arp2/3 complex-mediated actin polymerization by cortactinQ24290885
WIP regulates N-WASP-mediated actin polymerization and filopodium formationQ24291191
Reconstitution of human Arp2/3 complex reveals critical roles of individual subunits in complex structure and activityQ24291995
Cortactin interacts with WIP in regulating Arp2/3 activation and membrane protrusionQ24296813
Sequential interaction of actin-related proteins 2 and 3 (Arp2/3) complex with neural Wiscott-Aldrich syndrome protein (N-WASP) and cortactin during branched actin filament network formationQ24301359
Phosphorylation of the WASP-VCA domain increases its affinity for the Arp2/3 complex and enhances actin polymerization by WASPQ24302943
Phosphorylation of tyrosine 291 enhances the ability of WASp to stimulate actin polymerization and filopodium formation. Wiskott-Aldrich Syndrome proteinQ24306798
Wiskott-Aldrich syndrome protein, a novel effector for the GTPase CDC42Hs, is implicated in actin polymerizationQ24309118
Fission yeast Sop2p: a novel and evolutionarily conserved protein that interacts with Arp3p and modulates profilin functionQ24309489
SCAR, a WASP-related protein, isolated as a suppressor of receptor defects in late Dictyostelium developmentQ24311769
WIP, a protein associated with wiskott-aldrich syndrome protein, induces actin polymerization and redistribution in lymphoid cellsQ24315106
CR16 forms a complex with N-WASP in brain and is a novel member of a conserved proline-rich actin-binding protein familyQ30715777
Role of cofilin in epidermal growth factor-stimulated actin polymerization and lamellipod protrusionQ31494444
The Arp2/3 complex nucleates actin filament branches from the sides of pre-existing filamentsQ32084136
Cascade pathway of filopodia formation downstream of SCAR.Q33197463
Cytoskeleton in plant developmentQ33801776
Involvement of the Arp2/3 complex in phagocytosis mediated by FcgammaR or CR3.Q33899706
ARP3beta, the gene encoding a new human actin-related protein, is alternatively spliced and predominantly expressed in brain neuronal cells.Q33901563
The Arp2/3 complex branches filament barbed ends: functional antagonism with capping proteinsQ33908091
Activation of the Arp2/3 complex by the Listeria acta protein. Acta binds two actin monomers and three subunits of the Arp2/3 complex.Q33921214
Dynamics of the Dictyostelium Arp2/3 complex in endocytosis, cytokinesis, and chemotaxis.Q33957022
WICH, a novel verprolin homology domain-containing protein that functions cooperatively with N-WASP in actin-microspike formationQ34112829
WH2 domain: a small, versatile adapter for actin monomers.Q34120315
Identification and characterisation of a novel human isoform of Arp2/3 complex subunit p16-ARC/ARPC5.Q34160893
End versus side branching by Arp2/3 complexQ34184645
Molecular requirements for the internalisation step of endocytosis: insights from yeastQ34201680
Biological basket weaving: formation and function of clathrin-coated vesiclesQ34425195
Importance of free actin filament barbed ends for Arp2/3 complex function in platelets and fibroblasts.Q34429858
The plant cytoskeleton: vacuoles and cell walls make the differenceQ34495029
ATP hydrolysis on actin-related protein 2/3 complex causes debranching of dendritic actin arraysQ35142818
Dendritic organization of actin comet tailsQ57974906
Arabidopsis CROOKED encodes for the smallest subunit of the ARP2/3 complex and controls cell shape by region specific fine F-actin formationQ61730558
Caldesmon inhibits Arp2/3-mediated actin nucleationQ73128366
Roles of SLP-76, phosphoinositide 3-kinase, and gelsolin in the platelet shape changes initiated by the collagen receptor GPVI/FcR gamma-chain complexQ73219913
Yeast Eps15-like endocytic protein, Pan1p, activates the Arp2/3 complexQ74122048
Inhibition of the Arp2/3 complex-nucleated actin polymerization and branch formation by tropomyosinQ74431841
Two verprolin homology domains increase the Arp2/3 complex-mediated actin polymerization activities of N-WASP and WAVE1 C-terminal regionsQ74800786
Tyrosine phosphorylation of the Wiskott-Aldrich syndrome protein by Lyn and Btk is regulated by CDC42Q77318009
Evidence that the Wiskott-Aldrich syndrome protein may be involved in lymphoid cell signaling pathwaysQ24316097
Isolation of a novel gene mutated in Wiskott-Aldrich syndromeQ24317943
Induction of filopodium formation by a WASP-related actin-depolymerizing protein N-WASPQ24318478
Mammalian actin-related protein 2/3 complex localizes to regions of lamellipodial protrusion and is composed of evolutionarily conserved proteinsQ24324033
Recruitment of the Arp2/3 complex to vinculin: coupling membrane protrusion to matrix adhesionQ24324823
A WASp-VASP complex regulates actin polymerization at the plasma membrane.Q24535834
Motility determinants in WASP family proteinsQ24537166
Loss of WAVE-1 causes sensorimotor retardation and reduced learning and memory in miceQ24550753
Involvement of the Arp2/3 complex and Scar2 in Golgi polarity in scratch wound modelsQ24550805
Wiskott-Aldrich syndrome protein is associated with the adapter protein Grb2 and the epidermal growth factor receptor in living cellsQ24599228
Arp2/3 complex is required for actin polymerization during platelet shape changeQ24603149
Syndapin I, a synaptic dynamin-binding protein that associates with the neural Wiskott-Aldrich syndrome proteinQ24642969
The interaction of Arp2/3 complex with actin: nucleation, high affinity pointed end capping, and formation of branching networks of filamentsQ24653380
A novel neural Wiskott-Aldrich syndrome protein (N-WASP) binding protein, WISH, induces Arp2/3 complex activation independent of Cdc42Q24670450
Direct regulation of Arp2/3 complex activity and function by the actin binding protein coroninQ24671425
Purification of a cortical complex containing two unconventional actins from Acanthamoeba by affinity chromatography on profilin-agaroseQ24673164
Arp2/3 complex and actin depolymerizing factor/cofilin in dendritic organization and treadmilling of actin filament array in lamellipodiaQ24682164
Crystal structure of Arp2/3 complexQ27636380
The small GTP-binding protein rac regulates growth factor-induced membrane rufflingQ27861063
Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodiaQ27861126
The complex containing actin-related proteins Arp2 and Arp3 is required for the motility and integrity of yeast actin patches.Q27932211
Genetic dissection of the budding yeast Arp2/3 complex: a comparison of the in vivo and structural roles of individual subunitsQ27933020
The WASp homologue Las17p functions with the WIP homologue End5p/verprolin and is essential for endocytosis in yeastQ27933396
A novel fluorescence-activated cell sorter-based screen for yeast endocytosis mutants identifies a yeast homologue of mammalian eps15.Q27935226
A pathway for association of receptors, adaptors, and actin during endocytic internalization.Q27935237
Sla1p couples the yeast endocytic machinery to proteins regulating actin dynamicsQ27936077
A role for myosin-I in actin assembly through interactions with Vrp1p, Bee1p, and the Arp2/3 complexQ27936532
Bee1, a yeast protein with homology to Wiscott-Aldrich syndrome protein, is critical for the assembly of cortical actin cytoskeletonQ27938066
Pan1p, End3p, and S1a1p, three yeast proteins required for normal cortical actin cytoskeleton organization, associate with each other and play essential roles in cell wall morphogenesisQ27939792
Cdc42-interacting protein 4 mediates binding of the Wiskott-Aldrich syndrome protein to microtubulesQ28138296
A complex of N-WASP and WIP integrates signalling cascades that lead to actin polymerizationQ28139787
Reconstitution of actin-based motility of Listeria and Shigella using pure proteinsQ29617447
Guanine nucleotide exchange factors for Rho GTPases: turning on the switchQ29618403
Imaging actin and dynamin recruitment during invagination of single clathrin-coated pitsQ29620473
Negative regulation of yeast WASp by two SH3 domain-containing proteinsQ29870854
The Dictyostelium CARMIL protein links capping protein and the Arp2/3 complex to type I myosins through their SH3 domainsQ30168083
The SH3 domain of Bruton's tyrosine kinase displays altered ligand binding properties when auto-phosphorylated in vitroQ30175389
Membrane-targeting of signalling molecules by SH2/SH3 domain-containing adaptor proteinsQ30175408
Tyrosine phosphorylation regulates the SH3-mediated binding of the Wiskott-Aldrich syndrome protein to PSTPIP, a cytoskeletal-associated proteinQ30176255
Identification and characterization of a novel cytoskeleton-associated pp60src substrateQ30195583
Dynamin2 and cortactin regulate actin assembly and filament organization.Q30310193
A dynamin-cortactin-Arp2/3 complex mediates actin reorganization in growth factor-stimulated cellsQ30477658
Interaction of WASP/Scar proteins with actin and vertebrate Arp2/3 complexQ28142028
Integration of multiple signals through cooperative regulation of the N-WASP-Arp2/3 complexQ28142313
GRB2 links signaling to actin assembly by enhancing interaction of neural Wiskott-Aldrich syndrome protein (N-WASp) with actin-related protein (ARP2/3) complexQ28142883
Genomic organization and expression profile of the human and mouse WAVE gene familyQ28186686
Nck and phosphatidylinositol 4,5-bisphosphate synergistically activate actin polymerization through the N-WASP-Arp2/3 pathwayQ28187612
Endocytic protein intersectin-l regulates actin assembly via Cdc42 and N-WASPQ28190036
Cortactin promotes and stabilizes Arp2/3-induced actin filament network formationQ28206691
Structure of Arp2/3 complex in its activated state and in actin filament branch junctionsQ28215060
Cofilin produces newly polymerized actin filaments that are preferred for dendritic nucleation by the Arp2/3 complexQ28215459
The WRP component of the WAVE-1 complex attenuates Rac-mediated signallingQ28215904
Small GTPase Tc10 and its homologue RhoT induce N-WASP-mediated long process formation and neurite outgrowthQ28216274
Phosphatidylinositol 4,5-biphosphate (PIP2)-induced vesicle movement depends on N-WASP and involves Nck, WIP, and Grb2Q28216341
Neural Wiskott-Aldrich syndrome protein is recruited to rafts and associates with endophilin A in response to epidermal growth factorQ28217203
Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and NckQ28218186
The WASP-binding protein WIRE has a role in the regulation of the actin filament system downstream of the platelet-derived growth factor receptorQ28221039
Two GTPases, Cdc42 and Rac, bind directly to a protein implicated in the immunodeficiency disorder Wiskott-Aldrich syndromeQ28289697
Wiskott-Aldrich syndrome protein (WASp) is a binding partner for c-Src family protein-tyrosine kinasesQ28289727
Scar1 and the related Wiskott-Aldrich syndrome protein, WASP, regulate the actin cytoskeleton through the Arp2/3 complexQ28293934
Hemostatic, inflammatory, and fibroblast responses are blunted in mice lacking gelsolinQ28300043
Actin polymerization is induced by Arp2/3 protein complex at the surface of Listeria monocytogenesQ28301594
Arp2/3 complex requires hydrolyzable ATP for nucleation of new actin filamentsQ28344002
WAVE2 deficiency reveals distinct roles in embryogenesis and Rac-mediated actin-based motilityQ28507062
Regulated interactions between dynamin and the actin-binding protein cortactin modulate cell shapeQ28510777
Actin pedestal formation by enteropathogenic Escherichia coli and intracellular motility of Shigella flexneri are abolished in N-WASP-defective cellsQ28590934
WAVE2 is required for directed cell migration and cardiovascular developmentQ28594066
The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assemblyQ28609843
Activation by Cdc42 and PIP(2) of Wiskott-Aldrich syndrome protein (WASp) stimulates actin nucleation by Arp2/3 complexQ28609852
Different WASP family proteins stimulate different Arp2/3 complex-dependent actin-nucleating activitiesQ28609857
Mechanism of N-WASP activation by CDC42 and phosphatidylinositol 4, 5-bisphosphateQ28647599
A subset of dynamic actin rearrangements in Drosophila requires the Arp2/3 complexQ28756055
A two-tiered mechanism by which Cdc42 controls the localization and activation of an Arp2/3-activating motor complex in yeastQ28854265
Rho GTPases and their effector proteinsQ29547740
N-WASP, a novel actin-depolymerizing protein, regulates the cortical cytoskeletal rearrangement in a PIP2-dependent manner downstream of tyrosine kinasesQ29614248
The Ras-related protein Cdc42Hs and bradykinin promote formation of peripheral actin microspikes and filopodia in Swiss 3T3 fibroblastsQ29614253
Mechanism of filopodia initiation by reorganization of a dendritic networkQ29616648
Two tandem verprolin homology domains are necessary for a strong activation of Arp2/3 complex-induced actin polymerization and induction of microspike formation by N-WASP.Q35820542
Actin filament organization in the fish keratocyte lamellipodium.Q36235593
Structure, subunit topology, and actin-binding activity of the Arp2/3 complex from AcanthamoebaQ36259390
Fission yeast myosin-I, Myo1p, stimulates actin assembly by Arp2/3 complex and shares functions with WASpQ36316569
Molecular requirements for actin-based lamella formation in Drosophila S2 cellsQ36323318
Direct involvement of yeast type I myosins in Cdc42-dependent actin polymerizationQ36326253
Profilin enhances Cdc42-induced nucleation of actin polymerizationQ36328620
Activation of the Arp2/3 complex by the actin filament binding protein Abp1pQ36360344
Antigen receptor-induced activation and cytoskeletal rearrangement are impaired in Wiskott-Aldrich syndrome protein-deficient lymphocytesQ36375520
Scar, a WASp-related protein, activates nucleation of actin filaments by the Arp2/3 complexQ36450248
PIR121 regulates pseudopod dynamics and SCAR activity in DictyosteliumQ38350752
Requirements for Arabidopsis ATARP2 and ATARP3 during epidermal developmentQ38351681
Contingent phosphorylation/dephosphorylation provides a mechanism of molecular memory in WASP.Q40585986
Expression of WASP and Scar1/WAVE1 actin-associated proteins is differentially modulated during differentiation of HL-60 cellsQ40668627
Sustained activation of N-WASP through phosphorylation is essential for neurite extensionQ40690235
Spatial regulation of actin dynamics: a tropomyosin-free, actin-rich compartment at the leading edgeQ40692364
Identification of essential genes in cultured mammalian cells using small interfering RNAs.Q40756849
N-WASP deficiency reveals distinct pathways for cell surface projections and microbial actin-based motilityQ40777112
Involvement of wiskott-aldrich syndrome protein in B-cell cytoplasmic tyrosine kinase pathway.Q40968768
Mechanism of interaction of Acanthamoeba actophorin (ADF/Cofilin) with actin filamentsQ41666476
Direct observation of dendritic actin filament networks nucleated by Arp2/3 complex and WASP/Scar proteins.Q41734676
Three regions within ActA promote Arp2/3 complex-mediated actin nucleation and Listeria monocytogenes motilityQ41753152
SCAR is a primary regulator of Arp2/3-dependent morphological events in DrosophilaQ42118144
Gelsolin is a downstream effector of rac for fibroblast motilityQ42639554
Phosphatidylinositol 4,5-bisphosphate induces actin-based movement of raft-enriched vesicles through WASP-Arp2/3.Q42803796
Microtubule involvement in NIH 3T3 Golgi and MTOC polarity establishmentQ42806132
Rac and Cdc42 induce actin polymerization and G1 cell cycle progression independently of p65PAK and the JNK/SAPK MAP kinase cascadeQ42807716
Phosphorylation of WAVE downstream of mitogen-activated protein kinase signalingQ42810905
Association of cortactin with dynamic actin in lamellipodia and on endosomal vesicles.Q42834009
Wasp, the Drosophila Wiskott-Aldrich syndrome gene homologue, is required for cell fate decisions mediated by Notch signalingQ42944688
Activation of Arp2/3 complex by Wiskott-Aldrich Syndrome protein is linked to enhanced binding of ATP to Arp2.Q43764664
Hydrolysis of ATP by polymerized actin depends on the bound divalent cation but not profilinQ43847617
Interaction of cortactin and N-WASp with Arp2/3 complexQ44101124
A conserved amphipathic helix in WASP/Scar proteins is essential for activation of Arp2/3 complexQ44519511
Differential Roles of WAVE1 and WAVE2 in Dorsal and Peripheral Ruffle Formation for Fibroblast Cell MigrationQ44612166
Identification of functionally important residues of Arp2/3 complex by analysis of homology models from diverse speciesQ44751768
Actin-based motility of vaccinia virus mimics receptor tyrosine kinase signalling.Q45746416
Interactions of ADF/cofilin, Arp2/3 complex, capping protein and profilin in remodeling of branched actin filament networksQ46181690
The putative Arabidopsis arp2/3 complex controls leaf cell morphogenesisQ46295499
Interaction of human Arp2/3 complex and the Listeria monocytogenes ActA protein in actin filament nucleationQ46673303
Essential role of the C. elegans Arp2/3 complex in cell migration during ventral enclosureQ47069151
Kette regulates actin dynamics and genetically interacts with Wave and WaspQ47071019
Conserved interactions with cytoskeletal but not signaling elements are an essential aspect of Drosophila WASp functionQ47072157
Arp2/3-dependent pseudocleavage [correction of psuedocleavage] furrow assembly in syncytial Drosophila embryosQ47072232
Arabidopsis contains ancient classes of differentially expressed actin-related protein genesQ47579573
Mutations in actin-related proteins 2 and 3 affect cell shape development in ArabidopsisQ47788368
The Arabidopsis actin-related protein 2 (AtARP2) promoter directs expression in xylem precursor cells and pollenQ47911393
Characterization of the WAVE1 knock-out mouse: implications for CNS development.Q47950175
A WASp homolog powers actin polymerization-dependent motility of endosomes in vivo.Q52107797
The F-actin side binding activity of the Arp2/3 complex is essential for actin nucleation and lamellipod extensionQ57364210
P433issuePt 1
P407language of work or nameEnglishQ1860
P921main subjectprotein familyQ417841
Wiskott-Aldrich syndromeQ953638
P304page(s)1-17
P577publication date2004-05-01
P1433published inBiochemical JournalQ864221
P1476titleSignalling to actin assembly via the WASP (Wiskott-Aldrich syndrome protein)-family proteins and the Arp2/3 complex
P478volume380

Reverse relations

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Q30615575Tyrosine phosphorylation of WIP releases bound WASP and impairs podosome assembly in macrophages
Q36458860Use of virtual cell in studies of cellular dynamics
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