A quasi-quantitative dual multiplexed immunoblot method to simultaneously analyze ATM and H2AX Phosphorylation in human peripheral blood mononuclear cells

scientific article published on 14 May 2015

A quasi-quantitative dual multiplexed immunoblot method to simultaneously analyze ATM and H2AX Phosphorylation in human peripheral blood mononuclear cells is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.18632/ONCOSCIENCE.162
P932PMC publication ID4468340
P698PubMed publication ID26097887
P5875ResearchGate publication ID280032446

P50authorJan H BeumerQ49531403
P2093author name stringHussein Tawbi
Christopher J Bakkenist
John C Schmitz
R Kenneth Czambel
Pamela A Hershberger
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DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139Q28131715
Initiation of DNA fragmentation during apoptosis induces phosphorylation of H2AX histone at serine 139Q28139691
A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening AssaysQ28145712
DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociationQ28206029
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Modeling pharmacodynamic response to the poly(ADP-Ribose) polymerase inhibitor ABT-888 in human peripheral blood mononuclear cellsQ28477427
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Phase I trial of cremophor EL with bolus doxorubicin.Q31985007
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ATR-dependent phosphorylation and activation of ATM in response to UV treatment or replication fork stallingQ34653133
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Targeting the DNA repair defect of BRCA tumoursQ36162913
A cell-based screen identifies ATR inhibitors with synthetic lethal properties for cancer-associated mutations.Q36908567
Fit-for-purpose biomarker method validation for application in clinical trials of anticancer drugsQ37798159
Doxorubicin activates ATM-dependent phosphorylation of multiple downstream targets in part through the generation of reactive oxygen speciesQ38335343
The ATM kinase signaling induced by the low-energy β-particles emitted by (33)P is essential for the suppression of chromosome aberrations and is greater than that induced by the energetic β-particles emitted by (32)P.Q38583761
Radiation therapy induces the DNA damage response in peripheral bloodQ39117815
Selective killing of ATM- or p53-deficient cancer cells through inhibition of ATR.Q39559122
Irreversible chromosome damage accumulates rapidly in the absence of ATM kinase activityQ39991232
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Discovery of 4-{4-[(3R)-3-Methylmorpholin-4-yl]-6-[1-(methylsulfonyl)cyclopropyl]pyrimidin-2-yl}-1H-indole (AZ20): a potent and selective inhibitor of ATR protein kinase with monotherapy in vivo antitumor activityQ42713005
Promotion of DNA repair by nuclear IKKβ phosphorylation of ATM in response to genotoxic stimuliQ44270673
Phase I and pharmacokinetic study of intravenous irinotecan plus oral ciclosporin in patients with fuorouracil-refractory metastatic colon cancer.Q44362128
Simultaneous determination of gemcitabine and its main metabolite, dFdU, in plasma of patients with advanced non-small-cell lung cancer by high-performance liquid chromatography-tandem mass spectrometryQ46946669
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. 1979.Q49012176
Pharmacokinetic comparison of oral and intravenous etoposide in patients treated with the CHOEP-regimen for malignant lymphomas.Q51749692
Near-infrared fluorescence detection permits accurate imaging of loading controls for Western blot analysisQ80416832
[Application of two-color infrared fluorescence for detection of protein phosphorylation]Q82669749
A comparison of methods for the analysis of low abundance proteins in desmoid tumor cellsQ83858017
P433issue5
P407language of work or nameEnglishQ1860
P304page(s)542-554
P577publication date2015-05-14
P1433published inOncoscienceQ27725796
P1476titleA quasi-quantitative dual multiplexed immunoblot method to simultaneously analyze ATM and H2AX Phosphorylation in human peripheral blood mononuclear cells
P478volume2

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cites work (P2860)
Q42570959ATM serine-1981 phosphorylation is a plausible biomarker
Q91824813Antibody validation for Western blot: By the user, for the user
Q52655311LMTK3 confers chemo-resistance in breast cancer.
Q47100777Phase I study of veliparib in combination with gemcitabine
Q38796786The downregulation of the RNA-binding protein Staufen2 in response to DNA damage promotes apoptosis
Q58615653pRAD50: a novel and clinically applicable pharmacodynamic biomarker of both ATM and ATR inhibition identified using mass spectrometry and immunohistochemistry

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