Identification and Evaluation of Reference Genes for Accurate Transcription Normalization in Safflower under Different Experimental Conditions

scientific article published on 12 October 2015

Identification and Evaluation of Reference Genes for Accurate Transcription Normalization in Safflower under Different Experimental Conditions is …
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scholarly articleQ13442814

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P819ADS bibcode2015PLoSO..1040218L
P356DOI10.1371/JOURNAL.PONE.0140218
P932PMC publication ID4601734
P698PubMed publication ID26457898
P5875ResearchGate publication ID282815973

P50authorWei WuQ47939470
P2093author name stringQing Wang
Bo Hu
Feng Pan
Dandan Li
Hongchang Liu
P2860cites workMolecular Cloning and Expression Analysis of Genes Encoding Two Microsomal Oleate Desaturases (FAD2) from Safflower (Carthamus tinctorius L.)Q58336378
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Devolopmental and growth temperature regulation of omega-3 fatty acid desaturase genes in safflower (Carthamus tinctorius L.).Q39035019
Genome-wide identification and testing of superior reference genes for transcript normalization in ArabidopsisQ40388663
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Ribosomal 18S RNA prevails over glyceraldehyde-3-phosphate dehydrogenase and beta-actin genes as internal standard for quantitative comparison of mRNA levels in invasive and noninvasive human melanoma cell subpopulationsQ43690072
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The lack of a systematic validation of reference genes: a serious pitfall undervalued in reverse transcription-polymerase chain reaction (RT-PCR) analysis in plants.Q51887823
Stable internal reference genes for normalization of real-time RT-PCR in tobacco (Nicotiana tabacum) during development and abiotic stress.Q51916986
Isolation and Characterization of a Temperature-Regulated Microsomal Oleate Desaturase Gene (CtFAD2-1) from Safflower (Carthamus tinctorius L.)Q58336377
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genesQ24534395
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Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data setsQ29615465
Identification and evaluation of new reference genes in Gossypium hirsutum for accurate normalization of real-time quantitative RT-PCR dataQ30000967
A large and functionally diverse family of Fad2 genes in safflower (Carthamus tinctorius L.).Q31110351
Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR.Q33259658
Exercise induced stress in horses: selection of the most stable reference genes for quantitative RT-PCR normalizationQ33335920
Selection of internal control genes for quantitative real-time RT-PCR studies during tomato development processQ33395091
Normalization of qRT-PCR data: the necessity of adopting a systematic, experimental conditions-specific, validation of referencesQ33415275
Selection of reference genes for quantitative real-time PCR expression studies in the apomictic and sexual grass Brachiaria brizanthaQ33477648
Selection of reliable reference genes for gene expression studies in peach using real-time PCRQ33484407
Evaluation of putative reference genes for gene expression normalization in soybean by quantitative real-time RT-PCR.Q33507167
Validation of reference genes for quantitative RT-PCR studies of gene expression in perennial ryegrass (Lolium perenne L.)Q33525408
Selection of reference genes for qPCR in hairy root cultures of peanutQ34045146
Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems.Q34146463
Reference genes for accurate transcript normalization in citrus genotypes under different experimental conditionsQ34162754
Evaluation of new reference genes in papaya for accurate transcript normalization under different experimental conditionsQ34406685
Quantitative real-time RT-PCR--a perspective.Q34426536
Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR.Q34524574
Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stressQ34561245
Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstreamQ34683632
Identification and validation of reference genes for transcript normalization in strawberry (Fragaria × ananassa) defense responses.Q34935432
Real-time PCR: what relevance to plant studies?Q35811627
P275copyright licenseCreative Commons Attribution 4.0 InternationalQ20007257
P6216copyright statuscopyrightedQ50423863
P433issue10
P407language of work or nameEnglishQ1860
P304page(s)e0140218
P577publication date2015-10-12
P1433published inPLOS OneQ564954
P1476titleIdentification and Evaluation of Reference Genes for Accurate Transcription Normalization in Safflower under Different Experimental Conditions
P478volume10