Validation of reference genes for quantitative RT-PCR studies of gene expression in perennial ryegrass (Lolium perenne L.)

scientific article

Validation of reference genes for quantitative RT-PCR studies of gene expression in perennial ryegrass (Lolium perenne L.) is …
instance of (P31):
scholarly articleQ13442814

External links are
P6179Dimensions Publication ID1004789595
P356DOI10.1186/1471-2199-11-8
P932PMC publication ID2827471
P698PubMed publication ID20089196
P5875ResearchGate publication ID41101591

P50authorJohn RocheQ51724075
P2093author name stringPuthigae Sathish
Anthony Thrush
Julia M Lee
Danny J Donaghy
P2860cites workAccurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genesQ24534395
The ER repeat protein YT521-B localizes to a novel subnuclear compartmentQ24685879
Validating internal controls for quantitative plant gene expression studiesQ24804305
Primer3 on the WWW for general users and for biologist programmersQ27861030
Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assaysQ28140044
The intranuclear localization and function of YT521-B is regulated by tyrosine phosphorylationQ28264717
The lack of a systematic validation of reference genes: a serious pitfall undervalued in reverse transcription-polymerase chain reaction (RT-PCR) analysis in plants.Q51887823
Normalisation of real-time RT-PCR gene expression measurements in Arabidopsis thaliana exposed to increased metal concentrations.Q51892953
Selection of reference genes in mouse embryos and in differentiating human and mouse ES cells.Q52010644
What affects mRNA levels in leaves of field-grown aspen? A study of developmental and environmental influences.Q52098976
Eleven golden rules of quantitative RT-PCR.Q55050280
Distinctive transcriptome responses to adverse environmental conditions inZea maysLQ61235763
Identification and validation of endogenous reference genes for expression profiling of T helper cell differentiation by quantitative real-time RT-PCRQ77299611
Housekeeping gene expression in bovine liver is affected by physiological state, feed intake, and dietary treatmentQ80150885
Housekeeping genes as internal standards: use and limitsQ29302686
Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data setsQ29615465
Structure and expression profile of the sucrose synthase multigene family in Arabidopsis.Q30336342
The eEFIA gene family is differentially expressed in maize endospermQ30851112
Exploring valid reference genes for gene expression studies in Brachypodium distachyon by real-time PCR.Q30851279
An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry developmentQ33263600
Transcriptome analysis reveals season-specific rbcS gene expression profiles in diploid perennial ryegrass (Lolium perenne L.).Q33268416
The role of AtMUS81 in interference-insensitive crossovers in A. thalianaQ33294063
Identifying the most suitable endogenous control for determining gene expression in hearts from organ donorsQ33311575
Reference genes for normalization of gene expression studies in human osteoarthritic articular cartilageQ33316881
Molecular characterization and expression analysis of five different elongation factor 1 alpha genes in the flatfish Senegalese sole (Solea senegalensis Kaup): differential gene expression and thyroid hormones dependence during metamorphosisQ33317401
Selection of reference genes for quantitative real-time PCR in a rat asphyxial cardiac arrest modelQ33337982
Validation of internal control for gene expression study in soybean by quantitative real-time PCR.Q33345926
Normalisation genes for expression analyses in the brown alga model Ectocarpus siliculosusQ33360989
Identification of suitable endogenous control genes for microRNA gene expression analysis in human breast cancerQ33362180
Selection of internal control genes for quantitative real-time RT-PCR studies during tomato development processQ33395091
Identification and validation of reference genes for quantitative RT-PCR normalization in wheatQ33411039
Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems.Q34146463
Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR.Q34524574
Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stressQ34561245
Selection of reliable reference genes for qRT-PCR studies on cetacean fibroblast cultures exposed to OCs, PBDEs, and 17beta-estradiol.Q40002694
Genome-wide identification and testing of superior reference genes for transcript normalization in ArabidopsisQ40388663
Evaluation of suitable reference genes for gene expression studies in bovine muscular tissueQ43111668
Transcript abundance in yeast varies over six orders of magnitudeQ46484149
Sugars and circadian regulation make major contributions to the global regulation of diurnal gene expression in ArabidopsisQ46813402
A high throughput Agrobacterium tumefaciens-mediated transformation method for functional genomics of perennial ryegrass (Lolium perenne L.).Q46976602
Molecular identification of an Arabidopsis S-adenosylmethionine transporter. Analysis of organ distribution, bacterial expression, reconstitution into liposomes, and functional characterizationQ48084949
P921main subjectLolium perenneQ161722
P304page(s)8
P577publication date2010-01-20
P1433published inBMC Molecular BiologyQ15760954
P1476titleValidation of reference genes for quantitative RT-PCR studies of gene expression in perennial ryegrass (Lolium perenne L.).
P478volume11

Reverse relations

cites work (P2860)
Q35229241An RNA sequencing transcriptome analysis of the high-temperature stressed tall fescue reveals novel insights into plant thermotolerance
Q57121872Antioxidant responses and gene expression in perennial ryegrass (Lolium perenne L.) under cadmium stress
Q33821493Assessment and validation of a suite of reverse transcription-quantitative PCR reference genes for analyses of density-dependent behavioural plasticity in the Australian plague locust.
Q35053086Characterization of reference genes for RT-qPCR in the desert moss Syntrichia caninervis in response to abiotic stress and desiccation/rehydration
Q35823054Contrasting Effects of Long-Term Grazing and Clipping on Plant Morphological Plasticity: Evidence from a Rhizomatous Grass
Q35584969Differential expression of sirtuins in the aging rat brain
Q34782707Differential responses of CO2 assimilation, carbohydrate allocation and gene expression to NaCl stress in perennial ryegrass with different salt tolerance
Q41947744Does gibberellin biosynthesis play a critical role in the growth of Lolium perenne? Evidence from a transcriptional analysis of gibberellin and carbohydrate metabolic genes after defoliation
Q33670788Effect of ploidy increase on transgene expression: example from Citrus diploid cybrid and allotetraploid somatic hybrid expressing the EGFP gene
Q34273769Evaluation of candidate reference genes for gene expression normalization in Brassica juncea using real time quantitative RT-PCR.
Q34406685Evaluation of new reference genes in papaya for accurate transcript normalization under different experimental conditions
Q41443824Evaluation of reference genes for reverse transcription quantitative real-time PCR (RT-qPCR) studies in Silene vulgaris considering the method of cDNA preparation
Q86394300Growth responses of two tall fescue cultivars to Pb stress and their metal accumulation characteristics
Q34984264Heat shock transcriptional responses in an MC-Producing Cyanobacterium (Planktothrix agardhii) and its MC-deficient mutant under high light conditions
Q35803394Identification and Evaluation of Reference Genes for Accurate Transcription Normalization in Safflower under Different Experimental Conditions
Q36989974Identification and Validation of Reference Genes for RT-qPCR Analysis in Non-Heading Chinese Cabbage Flowers
Q36629962Identification and testing of reference genes for Sesame gene expression analysis by quantitative real-time PCR
Q33965005Identification and validation of quantitative real-time reverse transcription PCR reference genes for gene expression analysis in teak (Tectona grandis L.f.).
Q31157367Identification of candidate reference genes in perennial ryegrass for quantitative RT-PCR under various abiotic stress conditions
Q34999688Identification of reference genes for qRT-PCR analysis in Yesso scallop Patinopecten yessoensis
Q44939181Identification of reference genes suitable for qRT-PCR in grapevine and application for the study of the expression of genes involved in pterostilbene synthesis
Q91045010Identification of suitable controls for miRNA quantification in T-cells and whole blood cells in sepsis
Q33722997Identification of suitable reference genes for normalization of qPCR data in comparative transcriptomics analyses in the Triticeae
Q51275990Identification of the valid reference genes for quantitative RT-PCR in annual ryegrass (Lolium multiflorum) under salt stress.
Q85683708Induced systemic resistance responses in perennial ryegrass against Magnaporthe oryzae elicited by semi-purified surfactin lipopeptides and live cells of Bacillus amyloliquefaciens
Q45407080Nitrogen starvation, salt and heat stress in coffee (Coffea arabica L.): identification and validation of new genes for qPCR normalization
Q92121429Optimal use of statistical methods to validate reference gene stability in longitudinal studies
Q28749605Plants modify biological processes to ensure survival following carbon depletion: a Lolium perenne model
Q48235897Reference Gene Selection for Quantitative Real-Time Reverse-Transcriptase PCR in Annual Ryegrass (Lolium multiflorum) Subjected to Various Abiotic Stresses.
Q36259862Reference Gene Selection for qPCR Normalization of Kosteletzkya virginica under Salt Stress
Q39456566Reference gene selection for qPCR in Ammopiptanthus mongolicus under abiotic stresses and expression analysis of seven ROS-scavenging enzyme genes
Q33696597Reference gene selection for quantitative reverse transcription-polymerase chain reaction normalization during in vitro adventitious rooting in Eucalyptus globulus Labill
Q36096920Reference gene selection in the desert plant Eremosparton songoricum
Q38010394Reference genes for measuring mRNA expression.
Q38147050Reference genes in real-time PCR.
Q28488146Reference genes to study herbicide stress response in Lolium sp.: up-regulation of P450 genes in plants resistant to acetolactate-synthase inhibitors
Q35680349Screening for Suitable Reference Genes for Quantitative Real-Time PCR in Heterosigma akashiwo (Raphidophyceae).
Q36832463Selection and Validation of Appropriate Reference Genes for Quantitative Real-Time PCR Analysis of Gene Expression in Lycoris aurea
Q35118607Selection and validation of reference genes for gene expression analysis in switchgrass (Panicum virgatum) using quantitative real-time RT-PCR.
Q33354441Selection and validation of reference genes for quantitative RT-PCR expression studies of the non-model crop Musa
Q38942570Selection and validation of reference genes for target gene analysis with quantitative RT-PCR in leaves and roots of bermudagrass under four different abiotic stresses
Q37639712Selection of Candidate Reference Genes for Gene Expression Analysis in Kentucky Bluegrass (Poa pratensis L.) under Abiotic Stress
Q35972516Selection of Reference Genes for Gene Expression Normalization in Peucedanum praeruptorum Dunn under Abiotic Stresses, Hormone Treatments and Different Tissues
Q35901652Selection of Reference Genes for Real-Time Quantitative PCR in Pinus massoniana Post Nematode Inoculation
Q33756897Selection of housekeeping genes as internal controls for quantitative RT-PCR analysis of the veined rapa whelk (Rapana venosa).
Q91665538Selection of reference genes for flowering pathway analysis in the masting plants, Celmisia lyallii and Chionochloa pallens, under variable environmental conditions
Q34020382Selection of reference genes for gene expression studies in Siberian Apricot (Prunus sibirica L.) Germplasm using quantitative real-time PCR
Q87858405Selection of suitable soybean EF1α genes as internal controls for real-time PCR analyses of tissues during plant development and under stress conditions
Q50505358The validity of a reference gene is highly dependent on the experimental conditions in green alga Ulva linza.
Q36275953Transcriptome profilings of two tall fescue (Festuca arundinacea) cultivars in response to lead (Pb) stress
Q36221138Validation of Reference Genes for Accurate Normalization of Gene Expression in Lilium davidii var. unicolor for Real Time Quantitative PCR.
Q36051836Validation of Reference Genes for Gene Expression by Quantitative Real-Time RT-PCR in Stem Segments Spanning Primary to Secondary Growth in Populus tomentosa
Q36964552Validation of Reference Genes for RT-qPCR Studies of Gene Expression in Preharvest and Postharvest Longan Fruits under Different Experimental Conditions
Q33690034Validation of Suitable Reference Genes for RT-qPCR Data in Achyranthes bidentata Blume under Different Experimental Conditions
Q34123033Validation of a set of reference genes to study response to herbicide stress in grasses
Q49912171Validation of house-keeping genes for normalization of gene expression data during diurnal/circadian studies in rice by RT-qPCR.
Q35165453Validation of potential reference genes for qPCR in maize across abiotic stresses, hormone treatments, and tissue types
Q46130893Validation of reference genes for RT-qPCR studies of gene expression in banana fruit under different experimental conditions
Q39448925Validation of reference genes for gene expression analysis in Valsa mali var. mali using real-time quantitative PCR.
Q46091592Validation of reference genes for gene expression studies in peanut by quantitative real-time RT-PCR.
Q34431266Validation of reference genes for gene expression studies in virus-infected Nicotiana benthamiana using quantitative real-time PCR.
Q39607537Validation of suitable reference genes for gene expression analysis in the halophyte Salicornia europaea by real-time quantitative PCR.
Q34364270Validation of the β-amy1 transcription profiling assay and selection of reference genes suited for a RT-qPCR assay in developing barley caryopsis
Q35254876Vernalization mediated changes in the Lolium perenne transcriptome