Reference genes in real-time PCR.

scientific article

Reference genes in real-time PCR. is …
instance of (P31):
scholarly articleQ13442814
review articleQ7318358

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P356DOI10.1007/S13353-013-0173-X
P932PMC publication ID3825189
P698PubMed publication ID24078518
P5875ResearchGate publication ID257206654

P50authorMarcin RapaczQ46851569
P2093author name stringBartłomiej Kozera
P2860cites workAccurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genesQ24534395
Potential viral pathogenic mechanism for new variant inflammatory bowel diseaseQ24535473
Statistical modeling for selecting housekeeper genesQ24797672
Validating internal controls for quantitative plant gene expression studiesQ24804305
Selection and validation of endogenous reference genes using a high throughput approachQ24805039
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodQ25938999
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymeraseQ26778389
A new mathematical model for relative quantification in real-time RT-PCRQ27860781
Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assaysQ28140044
Simultaneous amplification and detection of specific DNA sequencesQ28185655
The MIQE guidelines: minimum information for publication of quantitative real-time PCR experimentsQ28236560
Guideline to reference gene selection for quantitative real-time PCRQ28237161
GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissuesQ28239983
Housekeeping genes as internal standards: use and limitsQ29302686
Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlationsQ29304271
Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data setsQ29615465
A sequence-based approach to identify reference genes for gene expression analysisQ30496278
Selection and validation of reference genes for real-time RT-PCR studies in the non-model species Delomys sublineatus, an endemic Brazilian rodent.Q30976552
The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalizationQ30997390
Pitfalls in the normalization of real-time polymerase chain reaction dataQ31106076
Careful selection of reference genes is required for reliable performance of RT-qPCR in human normal and cancer cell linesQ31114457
Evidence based selection of housekeeping genesQ33299463
Selection and validation of reference genes for quantitative RT-PCR expression studies of the non-model crop MusaQ33354441
Instability of standard PCR reference genes in adipose-derived stem cells during propagation, differentiation and hypoxic exposureQ33381849
Identification of suitable internal control genes for expression studies in Coffea arabica under different experimental conditionsQ33397852
Identification and validation of reference genes for quantitative RT-PCR normalization in wheatQ33411039
Normalization of qRT-PCR data: the necessity of adopting a systematic, experimental conditions-specific, validation of referencesQ33415275
Selection of reference genes for quantitative real-time PCR expression studies in the apomictic and sexual grass Brachiaria brizanthaQ33477648
Selection of reliable reference genes for gene expression studies in peach using real-time PCRQ33484407
Validation of reference genes for quantitative expression analysis by real-time RT-PCR in Saccharomyces cerevisiaeQ33513760
Validation of internal reference genes for quantitative real-time PCR in a non-model organism, the yellow-necked mouse, Apodemus flavicollisQ33520550
Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybridaQ33522477
Real-time RT-PCR profiling of over 1400 Arabidopsis transcription factors: unprecedented sensitivity reveals novel root- and shoot-specific genesQ47781799
The lack of a systematic validation of reference genes: a serious pitfall undervalued in reverse transcription-polymerase chain reaction (RT-PCR) analysis in plants.Q51887823
SPUD: a quantitative PCR assay for the detection of inhibitors in nucleic acid preparations.Q54614648
Validation of housekeeping genes for normalizing RNA expression in real-time PCR.Q54708147
Towards a systematic validation of references in real-time rt-PCRQ61861153
Housekeeping gene variability in normal and cancerous colorectal, pancreatic, esophageal, gastric and hepatic tissuesQ81338869
Relationship of gene expression and chromosomal abnormalities in colorectal cancerQ82606356
The effects of cold, light and time of day during low-temperature shift on the expression of CBF6, FpCor14b and LOS2 in Festuca pratensisQ83126519
An alternatively spliced domain of the NDC1 NAD(P)H dehydrogenase gene strongly influences the expression of the ACTIN2 reference gene in Arabidopsis thalianaQ83126562
An improved qPCR protocol for rapid detection and quantification of Clostridium difficile in cattle fecesQ86091510
Validation of reference genes for quantitative RT-PCR studies of gene expression in perennial ryegrass (Lolium perenne L.)Q33525408
A practical approach to RT-qPCR-Publishing data that conform to the MIQE guidelinesQ33538911
Selection of reference genes for quantitative gene expression normalization in flax (Linum usitatissimum L.).Q33559169
Selection of optimal reference genes for normalization in quantitative RT-PCR.Q33578372
Validation of endogenous reference genes for qRT-PCR analysis of human visceral adipose samplesQ33584317
Validation of reference genes as internal control for studying viral infections in cereals by quantitative real-time RT-PCR.Q33632746
Reference gene selection for quantitative reverse transcription-polymerase chain reaction normalization during in vitro adventitious rooting in Eucalyptus globulus LabillQ33696597
Control selection for RNA quantitationQ34007791
Why the need for qPCR publication guidelines?--The case for MIQE.Q34089254
Evaluation of qPCR reference genes in two genotypes of Populus for use in photoperiod and low-temperature studiesQ34348305
Evaluation of new reference genes in papaya for accurate transcript normalization under different experimental conditionsQ34406685
Quantitative real-time RT-PCR--a perspective.Q34426536
Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stressQ34561245
How to perform RT-qPCR accurately in plant species? A case study on flower colour gene expression in an azalea (Rhododendron simsii hybrids) mapping populationQ34783573
Real-time PCR: what relevance to plant studies?Q35811627
Real-time RT-PCR normalisation; strategies and considerations.Q36091282
18S rRNA is a reliable normalisation gene for real time PCR based on influenza virus infected cellsQ36402301
RTPrimerDB: the portal for real-time PCR primers and probesQ37202820
The effects of cold acclimation on photosynthetic apparatus and the expression of COR14b in four genotypes of barley (Hordeum vulgare) contrasting in their tolerance to freezing and high-light treatment in cold conditionsQ37260680
A decade of improvements in quantification of gene expression and internal standard selectionQ37497149
How to do successful gene expression analysis using real-time PCR.Q37647080
Accurate and objective copy number profiling using real-time quantitative PCR.Q37671069
Development and validation of endogenous reference genes for expression profiling of medaka (Oryzias latipes) exposed to endocrine disrupting chemicals by quantitative real-time RT-PCR.Q40271652
MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experimentsQ42409958
Selection of appropriate reference genes for gene expression studies by quantitative real-time polymerase chain reaction in cucumberQ43224029
Evaluation of potential reference genes for use in gene expression studies in the conifer pathogen (Heterobasidion annosum).Q43543505
Normalization of reverse transcription quantitative-PCR with housekeeping genes in riceQ44694694
Evaluation of candidate reference genes for qPCR in maize.Q45130948
Reference gene selection for quantitative real-time PCR normalization in tomato subjected to nitrogen, cold, and light stress.Q46005227
mRNA and 18S-RNA coapplication-reverse transcription for quantitative gene expression analysisQ46684005
Single muscle fiber gene expression in human skeletal muscle: validation of internal control with exerciseQ47234444
P275copyright licenseCreative Commons Attribution-NonCommercial 2.0 GenericQ44128984
P6216copyright statuscopyrightedQ50423863
P433issue4
P921main subjectenzymeQ8047
eukaryoteQ19088
regulation of gene expressionQ411391
biomedical investigative techniqueQ66648976
regulation of plant gene expressionQ70575416
P304page(s)391-406
P577publication date2013-11-01
P1433published inJournal of Applied GeneticsQ15767059
P1476titleReference genes in real-time PCR
P478volume54

Reverse relations

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Q38907530Reference gene selection for reverse transcription quantitative polymerase chain reaction in chicken hypothalamus under different feeding status
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Q33705156Selection and Validation of Appropriate Reference Genes for Quantitative Real-Time PCR Normalization in Staminate and Perfect Flowers of Andromonoecious Taihangia rupestris
Q33843601Selection and Validation of Appropriate Reference Genes for qRT-PCR Analysis in Isatis indigotica Fort
Q59799521Selection and Validation of Novel RT-qPCR Reference Genes under Hormonal Stimuli and in Different Tissues of Santalum album
Q36317139Selection and Validation of Reference Genes for Accurate RT-qPCR Data Normalization in Coffea spp. under a Climate Changes Context of Interacting Elevated [CO2] and Temperature.
Q93012690Selection and Validation of Reference Genes for Gene Expression Studies in Codonopsis pilosula Based on Transcriptome Sequence Data
Q53686185Selection and evaluation of housekeeping genes as endogenous controls for quantification of mRNA transcripts in Theileria parva using quantitative real-time polymerase chain reaction (qPCR).
Q89819120Selection and validation of miR-1280 as a suitable endogenous normalizer for qRT-PCR Analysis of serum microRNA expression in Hepatocellular Carcinoma
Q38684836Selection and validation of reference genes for RT-qPCR indicates that juice of sugarcane varieties modulate the expression of C metabolism genes in the endophytic diazotrophic Herbaspirillum rubrisubalbicans strain HCC103.
Q47722663Selection and validation of reference genes for qRT-PCR analysis of gene expression in Microsporum canis growing under different adhesion-inducing conditions.
Q97549277Selection and validation of reliable reference genes for gene expression studies from Monilinia vaccinii-corymbosi infected wild blueberry phenotypes
Q61803809Selection of Reference Genes for Expression Analysis in Chinese Medicinal Herb
Q47651271Selection of Reference Genes for Expression Studies in Diaphorina citri (Hemiptera: Liviidae).
Q91707166Selection of Reference Genes for RT-qPCR Analysis Under Intrinsic Conditions in the Hawthorn Spider Mite, Amphitetranychus viennensis (Acarina: Tetranychidae)
Q92156788Selection of Reliable Reference Genes for Analysis of Gene Expression in Spinal Cord during Rat Postnatal Development and after Injury
Q57175035Selection of Reliable Reference Genes for RT-qPCR Analysis of Gene Expression From Different Habitats and Developmental Stages
Q36101411Selection of Suitable Reference Genes for RT-qPCR Normalization under Abiotic Stresses and Hormone Stimulation in Persimmon (Diospyros kaki Thunb).
Q47143735Selection of a suitable reference gene for quantitative gene expression in mouse lymph nodes after vaccination
Q47225029Selection of appropriate reference genes for the detection of rhythmic gene expression via quantitative real-time PCR in Tibetan hulless barley
Q47564187Selection of housekeeping genes and demonstration of RNAi in cotton leafhopper, Amrasca biguttula biguttula (Ishida).
Q90472778Selection of miRNA reference genes for plant defence studies in rice (Oryza sativa)
Q39916113Selection of reference genes for expression analysis in the entomophthoralean fungus Pandora neoaphidis
Q91665538Selection of reference genes for flowering pathway analysis in the masting plants, Celmisia lyallii and Chionochloa pallens, under variable environmental conditions
Q33687627Selection of reference genes for microRNA analysis associated to early stress response to handling and confinement in Salmo salar
Q90471966Selection of reference genes for normalization of RT-qPCR data in gene expression studies in Anthonomus eugenii Cano (Coleoptera: Curculionidae)
Q35222664Selection of reference genes for quantitative RT-PCR (RT-qPCR) analysis of rat tissues under physiological and toxicological conditions
Q55665084Selection of reference genes for quantitative real-time PCR analysis in halophytic plant Rhizophora apiculata.
Q41632989Selection of reference genes suitable for normalization of qPCR data under abiotic stresses in bioenergy crop Arundo donax L.
Q37019287Selection of reliable reference genes for RT-qPCR analysis during developmental stages and abiotic stress in Setaria viridis.
Q57090913Selection of stable expressed reference genes in native and vitrified/thawed human ovarian tissue for analysis by qRT-PCR and Western blot
Q33772868Selection of suitable endogenous reference genes for qPCR in kidney and hypothalamus of rats under testosterone influence
Q64065313Selection of suitable reference genes for gene expression analysis in gills and liver of fish under field pollution conditions
Q90858526Selection of suitable reference genes for gene expression studies in myxosporean (Myxozoa, Cnidaria) parasites
Q35556557Selection of suitable reference genes for qPCR normalization under abiotic stresses and hormone stimuli in carrot leaves
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Q40288838Stability Assessment of Candidate Reference Genes in Urine Sediment of Prostate Cancer Patients for miRNA Applications.
Q39189210Stable In Vivo Transgene Expression in Endothelial Cells with Helper-Dependent Adenovirus: Roles of Promoter and Interleukin-10.
Q33627725Stable Internal Reference Genes for Normalizing Real-Time Quantitative PCR in Baphicacanthus cusia under Hormonal Stimuli and UV Irradiation, and in Different Plant Organs.
Q36261258Stably Expressed Genes Involved in Basic Cellular Functions
Q39440650Standardization and quality control for high-dimensional mass cytometry studies of human samples
Q64063537Temperature-induced aerobic scope and Hsp70 expression in the sea cucumber Holothuria scabra
Q37356803The 60S ribosomal protein L13 is the most preferable reference gene to investigate gene expression in selected organs from turkeys and chickens, in context of different infection models
Q89896708The Hypoxic Proteome and Metabolome of Barley (Hordeum vulgare L.) with and without Phytoglobin Priming
Q89396771The Oomycete Pythium oligandrum Can Suppress and Kill the Causative Agents of Dermatophytoses
Q34499781The choice of reference genes for assessing gene expression in sugarcane under salinity and drought stresses
Q55262536The observed alteration in BCL2 expression following lithium treatment is influenced by the choice of normalization method.
Q91558906The role and robustness of the Gini coefficient as an unbiased tool for the selection of Gini genes for normalising expression profiling data
Q91866937Tissue Specific Reference Genes for MicroRNA Expression Analysis in a Mouse Model of Peripheral Nerve Injury
Q41760607Tissue-specific selection of optimal reference genes for expression analysis of anti-cancer drug-related genes in tumor samples using quantitative real-time RT-PCR.
Q30908177Tracking the best reference genes for RT-qPCR data normalization in filamentous fungi
Q40994358Transcription of putative tonoplast transporters in response to glyphosate and paraquat stress in Conyza bonariensis and Conyza canadensis and selection of reference genes for qRT-PCR.
Q61798266Transcriptome-based identification and validation of reference genes for plant-bacteria interaction studies using Nicotiana benthamiana
Q38668110Transcriptome-based identification of the optimal reference CHO genes for normalisation of qPCR data
Q52677062Transcriptome-wide selection of a reliable set of reference genes for gene expression studies in potato cyst nematodes (Globodera spp.).
Q36314305Use of RNA-seq data to identify and validate RT-qPCR reference genes for studying the tomato-Pseudomonas pathosystem
Q30000033Using RNA-Seq Data to Evaluate Reference Genes Suitable for Gene Expression Studies in Soybean
Q55334114Using RT-qPCR, Proteomics, and Microscopy to Unravel the Spatio-Temporal Expression and Subcellular Localization of Hordoindolines Across Development in Barley Endosperm.
Q47153505Valid gene expression normalization by RT-qPCR in studies on hPDL fibroblasts with focus on orthodontic tooth movement and periodontitis
Q54165469Validating candidate reference genes for qRT-PCR-based gene expression analysis in nonfunctioning pituitary adenomas.
Q31098469Validating reference genes using minimally transformed qpcr data: findings in human cortex and outcomes in schizophrenia.
Q38907728Validation of Housekeeping Genes to Study Human Gingival Stem Cells and Their In Vitro Osteogenic Differentiation Using Real-Time RT-qPCR.
Q90911012Validation of Reference Genes for Normalization of Relative qRT-PCR Studies in Papillary Thyroid Carcinoma
Q50627786Validation of Reference Genes in mRNA Expression Analysis Applied to the Study of Asthma.
Q37126323Validation of Suitable Reference Genes for Assessing Gene Expression of MicroRNAs in Lonicera japonica
Q40469535Validation of adequate endogenous reference genes for reverse transcription-qPCR studies in human post-mortem brain tissue of SIDS cases.
Q49912171Validation of house-keeping genes for normalization of gene expression data during diurnal/circadian studies in rice by RT-qPCR.
Q37402145Validation of reference genes for normalization of gene expression by qRT-PCR in a resveratrol-producing entophytic fungus (Alternaria sp. MG1).
Q38749406Validation of reference genes for quantitative gene expression analysis in experimental epilepsy
Q96685769Validation of reference genes for the normalization of RT-qPCR gene expression in Acanthamoeba spp
Q52568642d-Alanyl-d-Alanine Ligase as a Broad-Host-Range Counterselection Marker in Vancomycin-Resistant Lactic Acid Bacteria.
Q58757580lncRNAs in Non-Malignant Tissue Have Prognostic Value in Colorectal Cancer
Q92991021miR-26a-5p is a Stable Reference Gene for miRNA Studies in Chondrocytes from Developing Human Cartilage
Q30491790quantGenius: implementation of a decision support system for qPCR-based gene quantification
Q58113698Β-actin does not show the characteristics of a reference protein in human cortex

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