Scalable Production of a Multifunctional Protein (TSG-6) That Aggregates with Itself and the CHO Cells That Synthesize It. (scientific article published on 21 January 2016)

A novel secretory tumor necrosis factor-inducible protein (TSG-6) is a member of the family of hyaluronate binding proteins, closely related to the adhesion receptor CD44

Q24297519

Human multipotent stromal cells attenuate lipopolysaccharide-induced acute lung injury in mice via secretion of tumor necrosis factor-α-induced protein 6

Q24302245

Plasticity of the TSG-6 HA-binding loop and mobility in the TSG-6-HA complex revealed by NMR and X-ray crystallography

Q27646300

TSG-6 inhibits neutrophil migration via direct interaction with the chemokine CXCL8

Q28116959

Long pentraxin 3/tumor necrosis factor-stimulated gene-6 interaction: a biological rheostat for fibroblast growth factor 2-mediated angiogenesis

Q28118263

TSG-6 binds via its CUB_C domain to the cell-binding domain of fibronectin and increases fibronectin matrix assembly

Q28119026

Anti-inflammatory and chondroprotective effect of TSG-6 (tumor necrosis factor-alpha-stimulated gene-6) in murine models of experimental arthritis

Q28363374

Intravenous hMSCs improve myocardial infarction in mice because cells embolized in lung are activated to secrete the anti-inflammatory protein TSG-6

Q29620590

A novel allelic variant of the human TSG-6 gene encoding an amino acid difference in the CUB module. Chromosomal localization, frequency analysis, modeling, and expression

Q34114962

The link module from human TSG-6 inhibits neutrophil migration in a hyaluronan- and inter-alpha -inhibitor-independent manner.

Q34156381

Anti-inflammatory protein TSG-6 reduces inflammatory damage to the cornea following chemical and mechanical injury.

Q34165745

Human inter-α-inhibitor is a substrate for factor XIIIa and tissue transglutaminase

Q34218459

The N-terminal module of thrombospondin-1 interacts with the link domain of TSG-6 and enhances its covalent association with the heavy chains of inter-alpha-trypsin inhibitor.

Q34313838

A novel mechanism for the inhibition of hyaluronan biosynthesis by 4-methylumbelliferone

Q34326198

Towards a structure for a TSG-6.hyaluronan complex by modeling and NMR spectroscopy: insights into other members of the link module superfamily.

Q34395546

Glycoengineering: the effect of glycosylation on the properties of therapeutic proteins

Q34426870

Incorporation of pentraxin 3 into hyaluronan matrices is tightly regulated and promotes matrix cross-linking

Q34430984

TSG-6: a pluripotent inflammatory mediator?

Q34527768

TSG-6 protein, a negative regulator of inflammatory arthritis, forms a ternary complex with murine mast cell tryptases and heparin

Q35067896

Impact of aeration strategy on CHO cell performance during antibody production.

Q51756335

Biphasic addition strategy of hypoxanthine and thymidine for improving monoclonal antibody production.

Q51758701

Determining the molecular basis for the pH-dependent interaction between the link module of human TSG-6 and hyaluronan.

Q53577939

Single step protocol to purify recombinant proteins with low endotoxin contents.

Q54475193

Overexpression, purification, and refolding of link module from human TSG-6 in Escherichia coli: effect of temperature, media, and mutagenesis on lysine misincorporation at arginine AGA codons.

Q54584125

Enhanced neutrophil extravasation and rapid progression of proteoglycan-induced arthritis in TSG-6-knockout mice

Q61658616

TSG-6 inhibits osteoclast activity via an autocrine mechanism and is functionally synergistic with osteoprotegerin

Q62078407

Amelioration of collagen-induced arthritis in DBA/1J mice by recombinant TSG-6, a tumor necrosis factor/interleukin-1-inducible protein

Q73363913

Reduced susceptibility to collagen-induced arthritis in DBA/1J mice expressing the TSG-6 transgene

Q78301199

The inflammation-associated protein TSG-6 cross-links hyaluronan via hyaluronan-induced TSG-6 oligomers

Q35110999

Anti-inflammatory protein TSG-6 secreted by activated MSCs attenuates zymosan-induced mouse peritonitis by decreasing TLR2/NF-κB signaling in resident macrophages.

Q35112231

Mesenchymal stem/stromal cells (MSCs): role as guardians of inflammation

Q35665181

Cytokine-induced gene expression at the crossroads of innate immunity, inflammation and fertility: TSG-6 and PTX3/TSG-14.

Q35757187

TSG-6 potentiates the antitissue kallikrein activity of inter-alpha-inhibitor through bikunin release.

Q35855257

Constitutive expression of inter-α-inhibitor (IαI) family proteins and tumor necrosis factor-stimulated gene-6 (TSG-6) by human amniotic membrane epithelial and stromal cells supporting formation of the heavy chain-hyaluronan (HC-HA) complex.

Q35874524

Heavy chains of inter alpha inhibitor (IαI) inhibit the human complement system at early stages of the cascade.

Q36016836

Intravenous mesenchymal stem cells prevented rejection of allogeneic corneal transplants by aborting the early inflammatory response

Q36401080

Multipotent stromal cells are activated to reduce apoptosis in part by upregulation and secretion of stanniocalcin-1.

Q36547021

TSG-6 regulates bone remodeling through inhibition of osteoblastogenesis and osteoclast activation

Q36871656

Endotoxin limits in formulations for preclinical research

Q36936944

Inter-α-inhibitor impairs TSG-6-induced hyaluronan cross-linking

Q37226176

Phase-directed therapy: TSG-6 targeted to early inflammation improves bleomycin-injured lungs

Q37576082

A refined model for the TSG-6 link module in complex with hyaluronan: use of defined oligosaccharides to probe structure and function

Q37608450

Formation of hyaluronan- and versican-rich pericellular matrix is required for proliferation and migration of vascular smooth muscle cells

Q38326238

Glycolaldehyde-modified β-lactoglobulin AGEs are unable to stimulate inflammatory signaling pathways in RAGE-expressing human cell lines

Q39666734

Heparin promotes suspension adaptation process of CHO-TS28 cells by eliminating cell aggregation

Q39686931

GRP-78 secreted by tumor cells blocks the antiangiogenic activity of bortezomib

Q39807121

Characterization of complexes formed between TSG-6 and inter-alpha-inhibitor that act as intermediates in the covalent transfer of heavy chains onto hyaluronan.

Q40433167

Inhibitory effects of TSG-6 Link module on leukocyte-endothelial cell interactions in vitro and in vivo.

Q42639229

Coding sequence, exon-intron structure and chromosomal localization of murine TNF-stimulated gene 6 that is specifically expressed by expanding cumulus cell-oocyte complexes

Q42670609

Hyaluronan binding properties of a CD44 chimera containing the link module of TSG-6.

Q43993812

Cartilage-specific constitutive expression of TSG-6 protein (product of tumor necrosis factor alpha-stimulated gene 6) provides a chondroprotective, but not antiinflammatory, effect in antigen-induced arthritis

Q44124063

Peroxisome proliferator-activated receptor alpha negatively regulates T-bet transcription through suppression of p38 mitogen-activated protein kinase activation

Q44484556

Characterization of the interaction between tumor necrosis factor-stimulated gene-6 and heparin: implications for the inhibition of plasmin in extracellular matrix microenvironments.

Q46510509

P275

Creative Commons Attribution 4.0 International

copyrighted

issue

language of work or name

English

Q1860

P304

page(s)

e0147553

P577

publication date

2016-01-21

P1433

published in

PLOS One

Q564954

P1476

title

Scalable Production of a Multifunctional Protein (TSG-6) That Aggregates with Itself and the CHO Cells That Synthesize It

P478

volume

Reverse relations

cites work (P2860)

Q48104554	Emerging Roles of Tumor Necrosis Factor-Stimulated Gene-6 in the Pathophysiology and Treatment of Atherosclerosis
Q42024223	Human Amniotic Membrane Mesenchymal Stem Cells inhibit Neutrophil Extracellular Traps through TSG-6.
Q33620044	Intranasal MSC-derived A1-exosomes ease inflammation, and prevent abnormal neurogenesis and memory dysfunction after status epilepticus.
Q92423937	Liver-targeted delivery of TSG-6 by calcium phosphate nanoparticles for the management of liver fibrosis
Q28077427	Mesenchymal stromal cell-based therapy: Regulatory and translational aspects in gastroenterology
Q38709337	TSG-6 Downregulates IFN-Alpha and TNF-Alpha Expression by Suppressing IRF7 Phosphorylation in Human Plasmacytoid Dendritic Cells