Three-dimensional reconstruction and analysis of mitotic spindles from the yeast, Schizosaccharomyces pombe

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Three-dimensional reconstruction and analysis of mitotic spindles from the yeast, Schizosaccharomyces pombe is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1083/JCB.120.1.141
P932PMC publication ID2119489
P698PubMed publication ID8416984

P2093author name stringK L McDonald
J R McIntosh
R Ding
P2860cites workArchitecture of the microtubule component of mitotic spindles from Dictyostelium discoideum.Q52872528
A study of the chromosomes of the yeast Schizosachcharomyces pombe by light and electron microscopyQ67338998
The use of cell division cycle mutants to investigate the control of microtubule distribution in the fission yeast Schizosaccharomyces pombeQ67980916
Visualization of centromeric and nucleolar DNA in fission yeast by fluorescence in situ hybridizationQ68204399
The transition of cells of the fission yeast beta-tubulin mutant nda3-311 as seen by freeze-substitution electron microscopy. Requirement of functional tubulin for spindle pole body duplicationQ68556773
Mitosis in the fission yeast Schizosaccharomyces pombe as revealed by freeze-substitution electron microscopyQ93538743
Mitosis in the fission yeast Schizosaccharomyces pombe: a comparative study with light and electron microscopyQ93713298
MPS1 and MPS2: novel yeast genes defining distinct steps of spindle pole body duplicationQ27934422
Universal control mechanism regulating onset of M-phaseQ29616481
The Number of Chromosomes in SCHIZOSACCHAROMYCES POMBE: Light Microscopy of Stained PreparationsQ33270132
Novel potential mitotic motor protein encoded by the fission yeast cut7+ geneQ34174859
Beyond self-assembly: from microtubules to morphogenesisQ34181655
Ultrastructure and time course of mitosis in the fungus Fusarium oxysporumQ36194412
Mitosis in the fungus Thraustotheca clavataQ36195813
Spindle and kinetochore morphology of Dictyostelium discoideumQ36197683
On the mechanism of anaphase spindle elongation in Diatoma vulgareQ36199347
Three-dimensional structure of the central mitotic spindle of Diatoma vulgareQ36203844
Cross-sectional structure of the central mitotic spindle of Diatoma vulgare. Evidence for specific interactions between antiparallel microtubulesQ36203857
Tubulin dynamics in cultured mammalian cellsQ36212188
In vitro reactivation of spindle elongation in fission yeast nuc2 mutant cellsQ36222582
In vitro microtubule-nucleating activity of spindle pole bodies in fission yeast Schizosaccharomyces pombe: cell cycle-dependent activation in xenopus cell-free extractsQ36531430
Kinetochore microtubules in PTK cellsQ36533211
Ultrastructure of mitotic spindles isolated from a cell division cycle mutant of the yeast, Saccharomyces cerevisiae.Q36627696
Functional analysis of a centromere from fission yeast: a role for centromere-specific repeated DNA sequencesQ36707744
Chromosome instability mutants of Saccharomyces cerevisiae that are defective in microtubule-mediated processesQ36713621
Electron-microscopic study of the spindle and chromosome movement in the yeast Saccharomyces cerevisiaeQ36724763
Advantages of fast-freeze fixation followed by freeze-substitution for the preservation of cell integrityQ37386810
Centromere structure and function in budding and fission yeastsQ37783138
The fission yeast, Schizosaccharomyces pombeQ37920664
A review of mitosis in the fission yeast Schizosaccharomyces pombeQ38709819
High-pressure freezing for the preservation of biological structure: theory and practiceQ38725037
Analysis of the distribution of spindle microtubules in the diatom FragilariaQ40979788
Hyphal tip cell ultrastructure of the fungus Fusarium: Improved preservation by freeze-substitutionQ41037039
Mitosis in the cellular slime mold Polysphondylium violaceumQ41066612
Absence of microtubule sliding and an analysis of spindle formation and elongation in isolated mitotic spindles from the yeast Saccharomyces cerevisiaeQ41442027
Studies on the mechanism of mitosisQ48454432
P433issue1
P407language of work or nameEnglishQ1860
P921main subject3D reconstructionQ4464732
Schizosaccharomyces pombeQ2236682
P1104number of pages11
P304page(s)141-151
P577publication date1993-01-01
P1433published inJournal of Cell BiologyQ1524550
P1476titleThree-dimensional reconstruction and analysis of mitotic spindles from the yeast, Schizosaccharomyces pombe
P478volume120

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cites work (P2860)
Q34354327A journey into space
Q30477485A mutation in gamma-tubulin alters microtubule dynamics and organization and is synthetically lethal with the kinesin-like protein pkl1p
Q36118983A new method reveals microtubule minus ends throughout the meiotic spindle
Q30496056A non-ring-like form of the Dam1 complex modulates microtubule dynamics in fission yeast
Q36382439A novel cis-acting centromeric DNA element affects S. pombe centromeric chromatin structure at a distance
Q35826040A stable microtubule array drives fission yeast polarity reestablishment upon quiescence exit
Q35841722A stochastic model of kinetochore-microtubule attachment accurately describes fission yeast chromosome segregation.
Q73294981A switch in microtubule dynamics at the onset of anaphase B in the mitotic spindle of Schizosaccharomyces pombe
Q36803193An allometric relationship between mitotic spindle width, spindle length, and ploidy in Caenorhabditis elegans embryos
Q47959633Analysis of the distribution of the kinetochore protein Ndc10p in Saccharomyces cerevisiae using 3-D modeling of mitotic spindles
Q28078705Anaphase B
Q80413415Anchoring microtubules at the spindle poles
Q30932835Ase1/Prc1-dependent spindle elongation corrects merotely during anaphase in fission yeast
Q30475810Ase1p organizes antiparallel microtubule arrays during interphase and mitosis in fission yeast
Q35464197Automated stitching of microtubule centerlines across serial electron tomograms
Q37024858Beyond the code: the mechanical properties of DNA as they relate to mitosis.
Q37234477Bi-orienting chromosomes: acrobatics on the mitotic spindle
Q38909098Brr6 drives the Schizosaccharomyces pombe spindle pole body nuclear envelope insertion/extrusion cycle.
Q30500057CaMtw1, a member of the evolutionarily conserved Mis12 kinetochore protein family, is required for efficient inner kinetochore assembly in the pathogenic yeast Candida albicans
Q35142905Captivating capture: how microtubules attach to kinetochores.
Q92144801Central-spindle microtubules are strongly coupled to chromosomes during both anaphase A and anaphase B
Q56866576Centromere and Kinetochore: Essential Components for Chromosome Segregation
Q34577187Centromeric heterochromatin: the primordial segregation machine
Q56866577Chromosome Components Important for Genome Stability in Candida albicans and Related Species
Q35127902Chromosome segregation: clamping down on deviant orientations.
Q98733629Closed mitosis requires local disassembly of the nuclear envelope
Q37786461Complex regulation of sister kinetochore orientation in meiosis-I
Q30487316Condensin regulates the stiffness of vertebrate centromeres
Q24312091Conservation of the centromere/kinetochore protein ZW10
Q30536163Conserved and divergent features of kinetochores and spindle microtubule ends from five species
Q50485606Contributions of Microtubule Dynamic Instability and Rotational Diffusion to Kinetochore Capture.
Q30657692Control of the spindle checkpoint by lateral kinetochore attachment and limited Mad1 recruitment
Q30736495Cryomethods for thin section electron microscopy
Q33275881Cryopreparation methods for electron microscopy of selected model systems
Q42421337Dual regulation of Mad2 localization on kinetochores by Bub1 and Dam1/DASH that ensure proper spindle interaction
Q74014193Dynamics of interphase microtubules in Schizosaccharomyces pombe
Q28242832Dynein promotes achiasmate segregation in Schizosaccharomyces pombe
Q36699175Electron microscopy and EM immunocytochemistry
Q39208061Electron microscopy for ultrastructural analysis and protein localization in Saccharomyces cerevisiae
Q28252959Electron tomography of cells
Q34694994Electron tomography of yeast cells
Q29616336Establishing biorientation occurs with precocious separation of the sister kinetochores, but not the arms, in the early spindle of budding yeast
Q33903518Finding the middle ground: how kinetochores power chromosome congression.
Q33703177Fission yeast a cellular model well suited for electron microscopy investigations
Q33630481Fission yeast hrp1, a chromodomain ATPase, is required for proper chromosome segregation and its overexpression interferes with chromatin condensation
Q36245141Fission yeast kinesin-8 controls chromosome congression independently of oscillations
Q40366403Fission yeast minichromosome loss mutants mis cause lethal aneuploidy and replication abnormality
Q37383024Fission yeast pkl1 is a kinesin-related protein involved in mitotic spindle function
Q44600815Freeze‐substitution protocols for improved visualization of membranes in high‐pressure frozen samples
Q53554834Gamma-tubulin complex-mediated anchoring of spindle microtubules to spindle-pole bodies requires Msd1 in fission yeast.
Q55382707High-quality ultrastructural preservation using cryofixation for 3D electron microscopy of genetically labeled tissues.
Q93086464High-resolution imaging reveals how the spindle midzone impacts chromosome movement
Q51616635ICRF-193, an anticancer topoisomerase II inhibitor, induces arched telophase spindles that snap, leading to a ploidy increase in fission yeast.
Q37780836Imaging and analysis of the microtubule cytoskeleton in giardia.
Q27935628Interactions between Centromere Complexes inSaccharomyces cerevisiae
Q50500643Interpolar microtubules are dispensable in fission yeast meiosis II.
Q36534704Interpolar spindle microtubules in PTK cells
Q95661011Kinesin-14 motors drive a right-handed helical motion of antiparallel microtubules around each other
Q33720854Kinesin-5-independent mitotic spindle assembly requires the antiparallel microtubule crosslinker Ase1 in fission yeast
Q64100038Kinesin-6 regulates cell-size-dependent spindle elongation velocity to keep mitosis duration constant in fission yeast
Q30834280Kinesin-8 effects on mitotic microtubule dynamics contribute to spindle function in fission yeast.
Q33980390Kinetochore and heterochromatin domains of the fission yeast centromere
Q36337950Kinetochore capture and bi-orientation on the mitotic spindle.
Q27939356Kinetochore recruitment of two nucleolar proteins is required for homolog segregation in meiosis I.
Q37598776Kinetochore targeting of fission yeast Mad and Bub proteins is essential for spindle checkpoint function but not for all chromosome segregation roles of Bub1p
Q30342478Kinetochore-microtubule interactions during cell division.
Q24602097Kinetochore-microtubule interactions: steps towards bi-orientation
Q36606737Kinetochore-microtubule interactions: the means to the end
Q27309203Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore.
Q30483272Latrunculin A delays anaphase onset in fission yeast by disrupting an Ase1-independent pathway controlling mitotic spindle stability
Q51066229M phase-specific kinetochore proteins in fission yeast: microtubule-associating Dis1 and Mtc1 display rapid separation and segregation during anaphase.
Q26991530MAPping the Ndc80 loop in cancer: A possible link between Ndc80/Hec1 overproduction and cancer formation
Q42015218Mal3, the fission yeast homologue of the human APC-interacting protein EB-1 is required for microtubule integrity and the maintenance of cell form
Q35529306Mechanical design principles of a mitotic spindle
Q37270763Mechanism controlling perpendicular alignment of the spindle to the axis of cell division in fission yeast
Q37242379Mechanisms of Mitotic Spindle Assembly
Q89693406Mechanisms of chromosome biorientation and bipolar spindle assembly analyzed by computational modeling
Q35968483Meiosis: cell-cycle controls shuffle and deal.
Q88932482Metaphase kinetochore movements are regulated by kinesin-8 motors and microtubule dynamic instability
Q30478152Microtubule depolymerization can drive poleward chromosome motion in fission yeast
Q88792656Microtubules grow by the addition of bent guanosine triphosphate tubulin to the tips of curved protofilaments
Q41370513Mini review: mitosis and the spindle pole body in Saccharomyces cerevisiae
Q34502388Mitosis and motor proteins in the filamentous ascomycete, Nectria haematococca, and some related fungi
Q24672896Mitosis, not just open or closed
Q34123562Mitosis: a history of division
Q37707186Mitotic force generators and chromosome segregation
Q37874698Mitotic moonlighting functions for membrane trafficking proteins
Q39446816Mitotic spindle: kinetochore fibers hold on tight to interpolar bundles
Q34818899Moe1, a conserved protein in Schizosaccharomyces pombe, interacts with a Ras effector, Scd1, to affect proper spindle formation
Q24535584Molecular analysis of kinetochore architecture in fission yeast
Q36660656Molecular architecture of the kinetochore-microtubule attachment site is conserved between point and regional centromeres
Q38723400Molecular model of fission yeast centrosome assembly determined by superresolution imaging
Q37167899Monopolin recruits condensin to organize centromere DNA and repetitive DNA sequences
Q33788429N-terminal regions of Mps1 kinase determine functional bifurcation
Q27934475Nbl1p: a Borealin/Dasra/CSC-1-like protein essential for Aurora/Ipl1 complex function and integrity in Saccharomyces cerevisiae
Q33415450Neocentromeres form efficiently at multiple possible loci in Candida albicans.
Q42090743Novel interactions of fission yeast kinesin 8 revealed through in vivo expression of truncation alleles
Q36941909Nuclear pore complex number and distribution throughout the Saccharomyces cerevisiae cell cycle by three-dimensional reconstruction from electron micrographs of nuclear envelopes
Q41845823Ordered kinetochore assembly in the human-pathogenic basidiomycetous yeast Cryptococcus neoformans
Q39695613Organization of the smallest eukaryotic spindle
Q27316212Paired arrangement of kinetochores together with microtubule pivoting and dynamics drive kinetochore capture in meiosis I
Q30489521Phosphorylation state defines discrete roles for monopolin in chromosome attachment and spindle elongation
Q40937747Phylogenesis of fission yeasts. Contradictions surrounding the origin of a century old genus
Q30835689Physical determinants of bipolar mitotic spindle assembly and stability in fission yeast.
Q46131158Pivoting of microtubules around the spindle pole accelerates kinetochore capture.
Q64282514Pivoting of microtubules driven by minus-end-directed motors leads to spindle assembly
Q41809145Plo1 phosphorylates Dam1 to promote chromosome bi-orientation in fission yeast.
Q36843155Poleward transport of Eg5 by dynein-dynactin in Xenopus laevis egg extract spindles
Q50464711Preparing Fission Yeast for Electron Microscopy
Q64066194Quantifying Tubulin Concentration and Microtubule Number Throughout the Fission Yeast Cell Cycle
Q42260386Quantitative single-molecule microscopy reveals that CENP-A(Cnp1) deposition occurs during G2 in fission yeast.
Q36238644RNA interference, heterochromatin, and centromere function.
Q32030422Reconstruction and display of curvilinear objects from optical section data using 3-D curve fitting algorithms
Q38023216Regulatory mechanisms of kinetochore-microtubule interaction in mitosis.
Q41043455Relevance of kinetochore size and microtubule-binding capacity for stable chromosome attachment during mitosis in PtK1 cells
Q37832683Ringing the changes: emerging roles for DASH at the kinetochore-microtubule Interface.
Q47805673Rsp1p, a J domain protein required for disassembly and assembly of microtubule organizing centers during the fission yeast cell cycle
Q27939597SET1, a yeast member of the trithorax family, functions in transcriptional silencing and diverse cellular processes
Q34193397Sim4: a novel fission yeast kinetochore protein required for centromeric silencing and chromosome segregation
Q44483999Sliding filaments and mitotic spindle organization
Q41828687Stabilization of overlapping microtubules by fission yeast CLASP.
Q33898838Stu1p is physically associated with beta-tubulin and is required for structural integrity of the mitotic spindle.
Q39105995Studying kinetochore-fiber ultrastructure using correlative light-electron microscopy
Q34097202Suppressors of Bir1p (Survivin) identify roles for the chromosomal passenger protein Pic1p (INCENP) and the replication initiation factor Psf2p in chromosome segregation
Q30476087The DASH complex and Klp5/Klp6 kinesin coordinate bipolar chromosome attachment in fission yeast
Q43092093The S. pombe mitotic regulator Cut12 promotes spindle pole body activation and integration into the nuclear envelope
Q97074387The Unusual Suspects in Cytokinesis: Fitting the Pieces Together
Q30476871The V260I mutation in fission yeast alpha-tubulin Atb2 affects microtubule dynamics and EB1-Mal3 localization and activates the Bub1 branch of the spindle checkpoint
Q28776781The centromere enhancer mediates centromere activation in Schizosaccharomyces pombe
Q34444031The centromeric K-type repeat and the central core are together sufficient to establish a functional Schizosaccharomyces pombe centromere
Q42530397The conserved Spc7 protein is required for spindle integrity and links kinetochore complexes in fission yeast
Q38627825The essentiality of the fungus-specific Dam1 complex is correlated with a one-kinetochore-one-microtubule interaction present throughout the cell cycle, independent of the nature of a centromere
Q39646913The fission yeast NIMA kinase Fin1p is required for spindle function and nuclear envelope integrity
Q35195721The fission yeast SPB component Cut12 links bipolar spindle formation to mitotic control.
Q27316044The fission yeast XMAP215 homolog Dis1p is involved in microtubule bundle organization
Q26767018The kinetochore interaction network (KIN) of ascomycetes
Q41977257The kinetochore proteins Pcs1 and Mde4 and heterochromatin are required to prevent merotelic orientation
Q34044917The microtubule organizing centers of Schizosaccharomyces pombe
Q58769069The mitotic spindle is chiral due to torques within microtubule bundles
Q27932270The molecular function of Ase1p: evidence for a MAP-dependent midzone-specific spindle matrix. Microtubule-associated proteins
Q24630956The monopolin complex crosslinks kinetochore components to regulate chromosome-microtubule attachments
Q104100296The phosphatase inhibitor Sds23 promotes symmetric spindle positioning in fission yeast
Q38049638The process of kinetochore assembly in yeasts
Q42771413The product of the spindle formation gene sad1+ associates with the fission yeast spindle pole body and is essential for viability
Q90408550The regulation of chromosome segregation via centromere loops
Q37980420The role of clathrin in mitotic spindle organisation
Q22065919The role of heterochromatin in centromere function
Q31846200The spindle cycle in budding yeast
Q27334674The spindle pole bodies facilitate nuclear envelope division during closed mitosis in fission yeast
Q37386890The spindle pole body of Schizosaccharomyces pombe enters and leaves the nuclear envelope as the cell cycle proceeds.
Q90211332Theory of Cytoskeletal Reorganization during Cross-Linker-Mediated Mitotic Spindle Assembly
Q30450971Three-dimensional analysis and ultrastructural design of mitotic spindles from the cdc20 mutant of Saccharomyces cerevisiae
Q41622762Three-dimensional electron microscopy analysis of ndc10-1 mutant reveals an aberrant organization of the mitotic spindle and spindle pole body defects in Saccharomyces cerevisiae
Q48925205Three-dimensional transmission electron microscopy and its application to mitosis research
Q24657131Three-dimensional ultrastructural analysis of the Saccharomyces cerevisiae mitotic spindle
Q36080983Total centromere size and genome size are strongly correlated in ten grass species
Q27931082Two microtubule-associated proteins required for anaphase spindle movement in Saccharomyces cerevisiae
Q35174775Ultrafine anaphase bridges, broken DNA and illegitimate recombination induced by a replication fork barrier
Q36746920Using electron microscopy to understand functional mechanisms of chromosome alignment on the mitotic spindle
Q35610621Using rapid freeze and freeze-substitution for the preparation of yeast cells for electron microscopy and three-dimensional analysis
Q33300359Vesicle-like biomechanics governs important aspects of nuclear geometry in fission yeast
Q79842388Whole-cell investigation of microtubule cytoskeleton architecture by electron tomography
Q48066966Xklp2, a novel Xenopus centrosomal kinesin-like protein required for centrosome separation during mitosis
Q41080335Yeast motor proteins
Q24672269Yin6, a fission yeast Int6 homolog, complexes with Moe1 and plays a role in chromosome segregation
Q34572239csi2p modulates microtubule dynamics and organizes the bipolar spindle for chromosome segregation.
Q36913732cut11(+): A gene required for cell cycle-dependent spindle pole body anchoring in the nuclear envelope and bipolar spindle formation in Schizosaccharomyces pombe
Q40241803p63cdc13, a B-type cyclin, is associated with both the nucleolar and chromatin domains of the fission yeast nucleus
Q33948516pkl1(+)and klp2(+): Two kinesins of the Kar3 subfamily in fission yeast perform different functions in both mitosis and meiosis

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