Engineering a Promiscuous Tautomerase into a More Efficient Aldolase for Self-Condensations of Linear Aliphatic Aldehydes

scientific article

Engineering a Promiscuous Tautomerase into a More Efficient Aldolase for Self-Condensations of Linear Aliphatic Aldehydes is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1002/CBIC.201700121
P932PMC publication ID5575498
P698PubMed publication ID28426139

P50authorGerrit J PoelarendsQ88902935
P2093author name stringMehran Rahimi
Edzard M Geertsema
Jan-Ytzen van der Meer
P2860cites workPromiscuity and electrostatic flexibility in the alkaline phosphatase superfamilyQ26771583
Evidence for the formation of an enamine species during aldol and Michael-type addition reactions promiscuously catalyzed by 4-oxalocrotonate tautomeraseQ27698082
A Promiscuous De Novo Retro-Aldolase Catalyzes Asymmetric Michael Additions via Schiff Base IntermediatesQ27698510
The 4-oxalocrotonate tautomerase family of enzymes: how nature makes new enzymes using a beta-alpha-beta structural motifQ28202879
Physically associated enzymes produce and metabolize 2-hydroxy-2,4-dienoate, a chemically unstable intermediate formed in catechol metabolism via meta cleavage in Pseudomonas putidaQ28354983
Addressing the numbers problem in directed evolutionQ33345289
The chemical versatility of the beta-alpha-beta fold: catalytic promiscuity and divergent evolution in the tautomerase superfamilyQ34095040
Expanding the enzyme universe: accessing non-natural reactions by mechanism-guided directed evolutionQ35504479
Chemomimetic biocatalysis: exploiting the synthetic potential of cofactor-dependent enzymes to create new catalystsQ35821520
Mutations Closer to the Active Site Improve the Promiscuous Aldolase Activity of 4-Oxalocrotonate Tautomerase More Effectively than Distant Mutations.Q36033515
Recent progress in stereoselective synthesis with aldolasesQ37673484
Recent advances in the study of enzyme promiscuity in the tautomerase superfamilyQ38104684
Recent developments in enzyme promiscuity for carbon-carbon bond-forming reactionsQ38322861
Using mutability landscapes of a promiscuous tautomerase to guide the engineering of enantioselective MichaelasesQ38917445
Demethionylation of Pro-1 variants of 4-oxalocrotonate tautomerase in Escherichia coli by co-expression with an engineered methionine aminopeptidaseQ41852843
Alteration of the diastereoselectivity of 3-methylaspartate ammonia lyase by using structure-based mutagenesisQ43294289
Promiscuous catalysis of asymmetric Michael-type additions of linear aldehydes to β-nitrostyrene by the proline-based enzyme 4-oxalocrotonate tautomeraseQ43778627
Asymmetric self- and cross-aldol reactions of glycolaldehyde catalyzed by D-fructose-6-phosphate aldolase.Q45953595
Inter- and intramolecular aldol reactions promiscuously catalyzed by a proline-based tautomeraseQ48145667
Biocatalytic Michael-type additions of acetaldehyde to nitroolefins with the proline-based enzyme 4-oxalocrotonate tautomerase yielding enantioenriched γ-nitroaldehydes.Q54784192
The Catalytic Asymmetric Aldol Reaction.Q55033822
Chemical and enzymic ketonization of 2-hydroxymuconate, a conjugated enolQ56815356
4-Oxalocrotonate tautomerase: pH dependence of catalysis and pKa values of active site residuesQ70862454
A simple ultraviolet spectrophotometric method for the determination of proteinQ74160877
Expanding the range of substrate acceptance of enzymes: combinatorial active-site saturation testQ81804014
Bridging between organocatalysis and biocatalysis: asymmetric addition of acetaldehyde to β-nitrostyrenes catalyzed by a promiscuous proline-based tautomeraseQ83100183
Systematic screening for catalytic promiscuity in 4-oxalocrotonate tautomerase: enamine formation and aldolase activityQ83361801
Enhancement of the promiscuous aldolase and dehydration activities of 4-oxalocrotonate tautomerase by protein engineeringQ84190408
An unexpected promiscuous activity of 4-oxalocrotonate tautomerase: the cis-trans isomerisation of nitrostyreneQ84694984
P433issue14
P304page(s)1435-1441
P577publication date2017-04-20
P1433published inChemBioChemQ634395
P1476titleEngineering a Promiscuous Tautomerase into a More Efficient Aldolase for Self-Condensations of Linear Aliphatic Aldehydes
P478volume18

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cites work (P2860)
Q89966600Enantioselective Aldol Addition of Acetaldehyde to Aromatic Aldehydes Catalyzed by Proline-Based Carboligases
Q89738262Selective Colorimetric "Turn-On" Probe for Efficient Engineering of Iminium Biocatalysis

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