Rapid production of gene replacement constructs and generation of a green fluorescent protein-tagged centromeric marker in Aspergillus nidulans

scientific article published on October 2004

Rapid production of gene replacement constructs and generation of a green fluorescent protein-tagged centromeric marker in Aspergillus nidulans is …
instance of (P31):
scholarly articleQ13442814

External links are
P356DOI10.1128/EC.3.5.1359-1362.2004
P932PMC publication ID522605
P698PubMed publication ID15470263

P50authorLin YangQ39941713
P2093author name stringStephen A Osmani
Xiaowei Dou
Jonathan Davies
Colin P C De Souza
Ariadna Perez-Balaguer
Leena Ukil
Aysha Osmani
Francis Nahm
P2860cites workThe Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregationQ24290969
A generic protein purification method for protein complex characterization and proteome explorationQ27861087
G1-phase and B-type cyclins exclude the DNA-replication factor Mcm4 from the nucleusQ27937350
A monomeric red fluorescent proteinQ28131782
Regulation of the mRNA levels of nimA, a gene required for the G2-M transition in Aspergillus nidulansQ36216204
Ribonuclease S-peptide as a carrier in fusion proteins.Q36278000
PCR-mediated generation of a gene disruption construct without the use of DNA ligase and plasmid vectorsQ39528384
A rapid method for efficient gene replacement in the filamentous fungus Aspergillus nidulansQ39601017
A mitogen-activated protein kinase (MPKA) is involved in polarized growth in the filamentous fungus, Aspergillus nidulansQ47968910
Peptide tags for a dual affinity fusion system.Q54633411
Characterization of an inducible expression system in Aspergillus nidulans using alcA and tubulin-coding genesQ62661216
Plant-adapted green fluorescent protein is a versatile vital reporter for gene expression, protein localization and mitosis in the filamentous fungus, Aspergillus nidulansQ74183921
P433issue5
P407language of work or nameEnglishQ1860
P921main subjectAspergillus nidulansQ683881
Putative component of the kinetochore-associated Ndc80 complexQ62640999
P304page(s)1359-1362
P577publication date2004-10-01
P1433published inEukaryotic CellQ5408685
P1476titleRapid production of gene replacement constructs and generation of a green fluorescent protein-tagged centromeric marker in Aspergillus nidulans
P478volume3

Reverse relations

cites work (P2860)
Q35829288A Duo of Potassium-Responsive Histidine Kinases Govern the Multicellular Destiny of Bacillus subtilis
Q34696285A high-throughput gene knockout procedure for Neurospora reveals functions for multiple transcription factors
Q37518047A mitotic nuclear envelope tether for Gle1 also impacts nuclear and nucleolar architecture.
Q36850207A novel automethylation reaction in the Aspergillus nidulans LaeA protein generates S-methylmethionine.
Q35221650A point mutation in the Aspergillus nidulans sonBNup98 nuclear pore complex gene causes conditional DNA damage sensitivity
Q57246154A two-step protocol for efficient deletion of genes in the filamentous ascomycete Podospora anserina
Q34587921A versatile and efficient gene-targeting system for Aspergillus nidulans.
Q26823169Advances in Aspergillus secondary metabolite research in the post-genomic era
Q37121983AgtA, the dicarboxylic amino acid transporter of Aspergillus nidulans, is concertedly down-regulated by exquisite sensitivity to nitrogen metabolite repression and ammonium-elicited endocytosis.
Q30513981An ordered pathway for the assembly of fungal ESCRT-containing ambient pH signalling complexes at the plasma membrane
Q41003511Analysis of a novel calcium auxotrophy in Aspergillus nidulans.
Q30487045Analysis of all protein phosphatase genes in Aspergillus nidulans identifies a new mitotic regulator, fcp1
Q27316996Application of a new dual localization-affinity purification tag reveals novel aspects of protein kinase biology in Aspergillus nidulans
Q36391844Approaches to functional genomics in filamentous fungi.
Q28276499Arp11 affects dynein-dynactin interaction and is essential for dynein function in Aspergillus nidulans
Q40646308Aspergillus nidulans Pmts form heterodimers in all pairwise combinations
Q53555987Aspergillus nidulans flippase DnfA is cargo of the endocytic collar and plays complementary roles in growth and phosphatidylserine asymmetry with another flippase, DnfB.
Q42090958Aspergillus nidulans protein O-mannosyltransferases play roles in cell wall integrity and developmental patterning
Q35024601Aspergillus nidulans transcription factor AtfA interacts with the MAPK SakA to regulate general stress responses, development and spore functions
Q36423335Basic-zipper-type transcription factor FlbB controls asexual development in Aspergillus nidulans
Q39451579Beyond asexual development: modifications in the gene expression profile caused by the absence of the Aspergillus nidulans transcription factor FlbB.
Q38654870Boron Tolerance in Aspergillus nidulans Is Sustained by the SltA Pathway Through the SLC-Family Transporters SbtA and SbtB.
Q30494857Characterization of Aspergillus nidulans DidB Did2, a non-essential component of the multivesicular body pathway
Q50528619Characterization of Aspergillus nidulans RabC/Rab6.
Q42974889Chitin synthase-deficient mutant of Fusarium oxysporum elicits tomato plant defence response and protects against wild-type infection
Q50218123Clathrin localization and dynamics in Aspergillus nidulans
Q33969020Co-ordination between BrlA regulation and secretion of the oxidoreductase FmqD directs selective accumulation of fumiquinazoline C to conidial tissues in Aspergillus fumigatus
Q52568755Construction of pDESTR, a GATEWAY vector for gene disruption in filamentous fungi.
Q30484840Copy number suppressors of the Aspergillus nidulans nimA1 mitotic kinase display distinctive and highly dynamic cell cycle-regulated locations.
Q27334279Cytoplasmic dynamics of the general nuclear import machinery in apically growing syncytial cells
Q33911093Cytoplasmic dynein's mitotic spindle pole localization requires a functional anaphase-promoting complex, gamma-tubulin, and NUDF/LIS1 in Aspergillus nidulans
Q48275177DASH-type cryptochromes regulate fruiting body development and secondary metabolism differently than CmWC-1 in the fungus Cordyceps militaris
Q50738037Depletion of Aspergillus nidulans cotA causes a severe polarity defect which is not suppressed by the nuclear migration mutation nudA2.
Q27305121Discovery of a vezatin-like protein for dynein-mediated early endosome transport
Q41952166Distinct ceramide synthases regulate polarized growth in the filamentous fungus Aspergillus nidulans
Q50443627Distinct mechanism of activation of two transcription factors, AmyR and MalR, involved in amylolytic enzyme production in Aspergillus oryzae.
Q28660761Distinct septin heteropolymers co-exist during multicellular development in the filamentous fungus Aspergillus nidulans
Q33851646Elucidation of functional markers from Aspergillus nidulans developmental regulator FlbB and their phylogenetic distribution
Q30413986Establishing a novel knock-in mouse line for studying neuronal cytoplasmic dynein under normal and pathologic conditions
Q33881399FHIP and FTS proteins are critical for dynein-mediated transport of early endosomes in Aspergillus
Q36244325FlbD, a Myb transcription factor of Aspergillus nidulans, is uniquely involved in both asexual and sexual differentiation
Q34625580Functional analysis of the Aspergillus nidulans kinome
Q34718516Functional characterization of Aspergillus nidulans homologues of Saccharomyces cerevisiae Spa2 and Bud6.
Q42547436Functional characterization of the putative Aspergillus nidulans poly(ADP-ribose) polymerase homolog PrpA
Q62658531Fusion PCR and gene targeting in Aspergillus nidulans
Q34739495G1/S transcription factor orthologues Swi4p and Swi6p are important but not essential for cell proliferation and influence hyphal development in the fungal pathogen Candida albicans
Q34070007Gamma-tubulin regulates the anaphase-promoting complex/cyclosome during interphase.
Q34336110GmcA is a putative glucose-methanol-choline oxidoreductase required for the induction of asexual development in Aspergillus nidulans
Q30576929HookA is a novel dynein-early endosome linker critical for cargo movement in vivo.
Q35273052How the necrotrophic fungus Alternaria brassicicola kills plant cells remains an enigma
Q61513255Identification and analysis of essential Aspergillus nidulans genes using the heterokaryon rescue technique
Q27316630Identification of interphase functions for the NIMA kinase involving microtubules and the ESCRT pathway
Q46152280Identification of virulence genes in Fusarium oxysporum f. sp. lycopersici by large-scale transposon tagging.
Q40945130In Aspergillus nidulans the suppressors suaA and suaC code for release factors eRF1 and eRF3 and suaD codes for a glutamine tRNA.
Q42633427In vivo analysis of the functions of gamma-tubulin-complex proteins
Q37412753Insights into dynamic mitotic chromatin organization through the NIMA kinase suppressor SonC, a chromatin-associated protein involved in the DNA damage response.
Q58775283Internuclear diffusion of histone H1 within cellular compartments of Aspergillus nidulans
Q28394173Laccases involved in 1,8-dihydroxynaphthalene melanin biosynthesis in Aspergillus fumigatus are regulated by developmental factors and copper homeostasis
Q41962866Lipoxygenase Activity Accelerates Programmed Spore Germination in Aspergillus fumigatus.
Q33827312Marker fusion tagging, a new method for production of chromosomally encoded fusion proteins
Q47978500Microtubule-organizing centers of Aspergillus nidulans are anchored at septa by a disordered protein
Q30840060Microtubules are reversibly depolymerized in response to changing gaseous microenvironments within Aspergillus nidulans biofilms
Q30574006Mitotic regulation of fungal cell-to-cell connectivity through septal pores involves the NIMA kinase
Q30487023Mlp1 acts as a mitotic scaffold to spatially regulate spindle assembly checkpoint proteins in Aspergillus nidulans
Q41282411Molecular cloning using polymerase chain reaction, an educational guide for cellular engineering
Q36807325Molecular genetic mining of the Aspergillus secondary metabolome: discovery of the emericellamide biosynthetic pathway
Q50804233Morphogenetic and developmental functions of the Aspergillus nidulans homologues of the yeast bud site selection proteins Bud4 and Axl2.
Q41637761Neurospora crassa ASM-1 complements the conidiation defect in a stuA mutant of Aspergillus nidulans
Q33753582Novel telomere-anchored PCR approach for studying sexual stage telomeres in Aspergillus nidulans
Q30495942Nuclear dynamics during germination, conidiation, and hyphal fusion of Fusarium oxysporum
Q42060064Nuclear export of the transcription factor NirA is a regulatory checkpoint for nitrate induction in Aspergillus nidulans
Q35340722Nuclear transporters in a multinucleated organism: functional and localization analyses in Aspergillus nidulans
Q30487020Nucleolar separation from chromosomes during Aspergillus nidulans mitosis can occur without spindle forces
Q34455685Nup2 requires a highly divergent partner, NupA, to fulfill functions at nuclear pore complexes and the mitotic chromatin region
Q35080863Orthologues of the anaphase-promoting complex/cyclosome coactivators Cdc20p and Cdh1p are important for mitotic progression and morphogenesis in Candida albicans
Q89996449Overlapping Roles in Chromosome Segregation for Heterochromatin Protein 1 (Swi6) and DDK in Schizosaccharomyces pombe
Q41937369PalC, one of two Bro1 domain proteins in the fungal pH signalling pathway, localizes to cortical structures and binds Vps32.
Q34821170Proteomic identification of potential target proteins regulated by the SCF(F) (bp1) -mediated proteolysis pathway in Fusarium oxysporum.
Q34131480QIP, a protein that converts duplex siRNA into single strands, is required for meiotic silencing by unpaired DNA.
Q41843361Ras GTPase-activating protein regulation of actin cytoskeleton and hyphal polarity in Aspergillus nidulans
Q28604239Refining the pH response in Aspergillus nidulans: a modulatory triad involving PacX, a novel zinc binuclear cluster protein
Q35161978Regulated inactivation of the spindle assembly checkpoint without functional mitotic spindles
Q36837015Regulation of apical dominance in Aspergillus nidulans hyphae by reactive oxygen species
Q48076477Regulation of hyphal morphogenesis by cdc42 and rac1 homologues in Aspergillus nidulans
Q37387814Regulation of mitosis by the NIMA kinase involves TINA and its newly discovered partner, An-WDR8, at spindle pole bodies
Q33853123Regulation of septum formation by the Bud3-Rho4 GTPase module in Aspergillus nidulans
Q34979362Regulation of sulphur assimilation is essential for virulence and affects iron homeostasis of the human-pathogenic mould Aspergillus fumigatus.
Q34336678Restraint of the G2/M transition by the SR/RRM family mRNA shuttling binding protein SNXAHRB1 in Aspergillus nidulans
Q28485158Secondary metabolism and development is mediated by LlmF control of VeA subcellular localization in Aspergillus nidulans
Q33585954Septins AspA and AspC are important for normal development and limit the emergence of new growth foci in the multicellular fungus Aspergillus nidulans
Q30494334Single-step affinity purification for fungal proteomics.
Q35019961Spatial regulation of the spindle assembly checkpoint and anaphase-promoting complex in Aspergillus nidulans
Q34454789Sumoylation in Aspergillus nidulans: sumO inactivation, overexpression and live-cell imaging
Q27933958Survival strategies of yeast and filamentous fungi against the antifungal protein AFP.
Q30478399Systematic deletion and mitotic localization of the nuclear pore complex proteins of Aspergillus nidulans.
Q42708095Telomere position effect is regulated by heterochromatin-associated proteins and NkuA in Aspergillus nidulans.
Q53262169The Aspergillus nidulans CENP-E kinesin motor KipA interacts with the fungal homologue of the centromere-associated protein CENP-H at the kinetochore.
Q51189818The Aspergillus nidulans Pbp1 homolog is required for normal sexual development and secondary metabolism.
Q43032146The DE loop of the domain III of the envelope protein appears to be associated with West Nile virus neutralization
Q64105216The Early Asexual Development Regulator Codes for a Putative Bifunctional Enzyme
Q34511507The Janus transcription factor HapX controls fungal adaptation to both iron starvation and iron excess
Q92921900The Kinetochore Protein Spc105, a Novel Interaction Partner of LaeA, Regulates Development and Secondary Metabolism in Aspergillus flavus
Q30570500The NIMA kinase is required to execute stage-specific mitotic functions after initiation of mitosis
Q35738986The Polo-like kinase PLKA in Aspergillus nidulans is not essential but plays important roles during vegetative growth and development
Q34016589The Set1/COMPASS histone H3 methyltransferase helps regulate mitosis with the CDK1 and NIMA mitotic kinases in Aspergillus nidulans
Q35573330The SrkA Kinase Is Part of the SakA Mitogen-Activated Protein Kinase Interactome and Regulates Stress Responses and Development in Aspergillus nidulans
Q30479680The WW domain protein PRO40 is required for fungal fertility and associates with Woronin bodies
Q64954581The actin capping protein in Aspergillus nidulans enhances dynein function without significantly affecting Arp1 filament assembly.
Q34334002The akuB(KU80) mutant deficient for nonhomologous end joining is a powerful tool for analyzing pathogenicity in Aspergillus fumigatus
Q44688852The cdr1B efflux transporter is associated with non-cyp51a-mediated itraconazole resistance in Aspergillus fumigatus
Q27309095The functions of myosin II and myosin V homologs in tip growth and septation in Aspergillus nidulans
Q46966220The interplay between the GATA transcription factors AreA, the global nitrogen regulator and AreB in Fusarium fujikuroi
Q46441464The membrane mucin Msb2 regulates invasive growth and plant infection in Fusarium oxysporum
Q39756257The nuclear migration protein NUDF/LIS1 forms a complex with NUDC and BNFA at spindle pole bodies.
Q28241892The p25 subunit of the dynactin complex is required for dynein-early endosome interaction
Q30506385The septin AspB in Aspergillus nidulans forms bars and filaments and plays roles in growth emergence and conidiation
Q30485471The three fungal transmembrane nuclear pore complex proteins of Aspergillus nidulans are dispensable in the presence of an intact An-Nup84-120 complex
Q30481634The tip growth apparatus of Aspergillus nidulans
Q43071887The two-component histidine kinase Fhk1 controls stress adaptation and virulence of Fusarium oxysporum
Q37225969Timely septation requires SNAD-dependent spindle pole body localization of the septation initiation network components in the filamentous fungus Aspergillus nidulans.
Q50571889Tip-to-nucleus migration dynamics of the asexual development regulator FlbB in vegetative cells.
Q33231909TmpA, a member of a novel family of putative membrane flavoproteins, regulates asexual development in Aspergillus nidulans.
Q99637205Tolerance to alkaline ambient pH in Aspergillus nidulans depends on the activity of ENA proteins
Q47342430Tools for retargeting proteins within Aspergillus nidulans.
Q36988663Toward awakening cryptic secondary metabolite gene clusters in filamentous fungi
Q64990377Transformation of Penicillium rubens 212 and Expression of GFP and DsRED Coding Genes for Visualization of Plant-Biocontrol Agent Interaction.
Q60949416UrdA Controls Secondary Metabolite Production and the Balance between Asexual and Sexual Development in
Q46818524VeA of Aspergillus niger increases spore dispersing capacity by impacting conidiophore architecture
Q57021983p25 of the dynactin complex plays a dual role in cargo binding and dynactin regulation
Q36205298γ-Tubulin plays a key role in inactivating APC/C(Cdh1) at the G(1)-S boundary

Search more.