Increased sensitivity and accuracy of a single-stranded DNA splint-mediated ligation assay (sPAT) reveals poly(A) tail length dynamics of developmentally regulated mRNAs

scientific article published on 10 February 2014

Increased sensitivity and accuracy of a single-stranded DNA splint-mediated ligation assay (sPAT) reveals poly(A) tail length dynamics of developmentally regulated mRNAs is …
instance of (P31):
scholarly articleQ13442814

External links are
P356DOI10.4161/RNA.27992
P932PMC publication ID3973730
P698PubMed publication ID24526206
P5875ResearchGate publication ID260195908

P2093author name stringChristian R Eckmann
David Rudel
Ryuji Minasaki
P2860cites workThe genetics of Caenorhabditis elegansQ24533408
ePAT: a simple method to tag adenylated RNA to measure poly(A)-tail length and other 3' RACE applicationsQ24617775
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Pathway to totipotency: lessons from germ cellsQ34585032
The GLD-2 poly(A) polymerase activates gld-1 mRNA in the Caenorhabditis elegans germ lineQ35108098
Cytoplasmic polyadenylation element (CPE)- and CPE-binding protein (CPEB)-independent mechanisms regulate early class maternal mRNA translational activation in Xenopus oocytesQ35672343
Messenger RNA deadenylylation precedes decapping in mammalian cellsQ36153241
Rapid and sensitive analysis of mRNA polyadenylation states by PCRQ36684068
Two conserved regulatory cytoplasmic poly(A) polymerases, GLD-4 and GLD-2, regulate meiotic progression in C. elegansQ37152250
FBF and its dual control of gld-1 expression in the Caenorhabditis elegans germlineQ37152671
C. elegans star proteins, GLD-1 and ASD-2, regulate specific RNA targets to control developmentQ37824403
Cytoplasmic polyadenylation and translational control.Q37871177
Subcellular specialization of multifaceted 3'end modifying nucleotidyltransferasesQ38007068
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A new G-tailing method for the determination of the poly(A) tail length applied to hepatitis A virus RNA.Q39095110
A conserved RNA-binding protein controls germline stem cells in Caenorhabditis elegansQ39750479
Spacing constraints on reinitiation of paramyxovirus transcription: the gene end U tract acts as a spacer to separate gene end from gene start sitesQ40858075
Direct comparison of nick-joining activity of the nucleic acid ligases from bacteriophage T4.Q42004423
Poly(A) addition during maturation of frog oocytes: distinct nuclear and cytoplasmic activities and regulation by the sequence UUUUUAU.Q42097279
The Ccr4-Not deadenylase complex constitutes the main poly(A) removal activity in C. elegansQ46989010
A regulatory cytoplasmic poly(A) polymerase in Caenorhabditis elegansQ47068695
Phosphorylation of CPE binding factor by Eg2 regulates translation of c-mos mRNA.Q48893248
Genetic regulation of entry into meiosis in Caenorhabditis elegans.Q48939088
Gonadogenesis in Pristionchus pacificus and organ evolution: development, adult morphology and cell-cell interactions in the hermaphrodite gonad.Q52085307
Coordinate initiation of Drosophila development by regulated polyadenylation of maternal messenger RNAs.Q52539712
Analysis of RNA-protein complexes by RNA coimmunoprecipitation and RT-PCR analysis from Caenorhabditis elegans.Q54441960
Determination of the poly(A) tail lengths of a single mRNA species in total hepatic RNAQ72308456
Joining RNA molecules with T4 DNA ligaseQ73151761
Analysis of poly(A) tail lengths by PCR: the PAT assayQ73151841
Assaying the polyadenylation state of mRNAsQ74602849
Multifunctional deadenylase complexes diversify mRNA controlQ80854891
P4510describes a project that usesImageJQ1659584
P433issue2
P304page(s)111-123
P577publication date2014-02-10
P1433published inRNA BiologyQ7277167
P1476titleIncreased sensitivity and accuracy of a single-stranded DNA splint-mediated ligation assay (sPAT) reveals poly(A) tail length dynamics of developmentally regulated mRNAs
P478volume11

Reverse relations

cites work (P2860)
Q35556686Analysis of circadian regulation of poly(A)-tail length
Q40950194Essential role for non-canonical poly(A) polymerase GLD4 in cytoplasmic polyadenylation and carbohydrate metabolism
Q27313226GLD-4-mediated translational activation regulates the size of the proliferative germ cell pool in the adult C. elegans germ line
Q35837062High-Throughput Profiling of Caenorhabditis elegans Starvation-Responsive microRNAs.
Q35150997Kleat: cleavage site analysis of transcriptomes
Q50128167Poly A tail length analysis of in vitro transcribed mRNA by LC-MS.
Q50438573Polyadenylation is the key aspect of GLD-2 function in C. elegans.
Q34482916Structural basis for the activation of the C. elegans noncanonical cytoplasmic poly(A)-polymerase GLD-2 by GLD-3
Q34313050The cytoplasmic poly(A) polymerases GLD-2 and GLD-4 promote general gene expression via distinct mechanisms
Q37367215Transcriptome-wide effects of inverted SINEs on gene expression and their impact on RNA polymerase II activity.
Q36106636Translational activation maintains germline tissue homeostasis during adulthood

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