Towards dynamic metabolic flux analysis in CHO cell cultures

scientific article

Towards dynamic metabolic flux analysis in CHO cell cultures is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1002/BIOT.201100052
P698PubMed publication ID22102428
P5875ResearchGate publication ID51816568

P2093author name stringMaciek R Antoniewicz
Woo Suk Ahn
P2860cites workEffects of ammonia and lactate on growth, metabolism, and productivity of BHK cellsQ58754717
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Determination of confidence intervals of metabolic fluxes estimated from stable isotope measurements.Q51220949
Dynamic metabolic flux analysis (DMFA): a framework for determining fluxes at metabolic non-steady state.Q51518128
Metabolic flux analysis of CHO cell metabolism in the late non-growth phase.Q51772488
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Effect of culture temperature on erythropoietin production and glycosylation in a perfusion culture of recombinant CHO cells.Q51785246
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The enzymic hydrolysis of glutamine and its spontaneous decomposition in buffer solutionsQ39428921
Metabolic flux analysis of CHO cells at growth and non-growth phases using isotopic tracers and mass spectrometryQ39494168
Development of a mathematical model for evaluating the dynamics of normal and apoptotic Chinese hamster ovary cellsQ39535172
Hexokinase 2 is a key mediator of aerobic glycolysis and promotes tumor growth in human glioblastoma multiformeQ39606772
Quantitative characterization of metabolism and metabolic shifts during growth of the new human cell line AGE1.HN using time resolved metabolic flux analysis.Q39615011
CHO-K1 host cells adapted to growth in glutamine-free medium by FACS-assisted evolutionQ39646825
The glucose transporter of mammalian cellsQ39819660
Expression of anti-apoptosis genes alters lactate metabolism of Chinese Hamster Ovary cells in cultureQ39880044
Quantifying reductive carboxylation flux of glutamine to lipid in a brown adipocyte cell lineQ39998829
Functional expression of human pyruvate carboxylase for reduced lactic acid formation of Chinese hamster ovary cells (DG44).Q40116841
Down-regulation of lactate dehydrogenase-A by siRNAs for reduced lactic acid formation of Chinese hamster ovary cells producing thrombopoietinQ40211655
Antibody productionQ40258663
Considerations on the lactate consumption by CHO cells in the presence of galactoseQ40258669
Metabolism of PER.C6 cells cultivated under fed-batch conditions at low glucose and glutamine levelsQ40307631
Effects of lactate dehydrogenase suppression and glycerol-3-phosphate dehydrogenase overexpression on cellular metabolismQ40316588
Impact of dynamic online fed-batch strategies on metabolism, productivity and N-glycosylation quality in CHO cell cultures.Q40461540
Impact of temperature reduction and expression of yeast pyruvate carboxylase on hGM-CSF-producing CHO cellsQ40570353
Large scale gene expression profiling of metabolic shift of mammalian cells in cultureQ40605473
Expression of recombinant cytoplasmic yeast pyruvate carboxylase for the improvement of the production of human erythropoietin by recombinant BHK-21 cellsQ40760951
Engineering of a mammalian cell line for reduction of lactate formation and high monoclonal antibody productionQ40841544
Glucose-6-phosphate dehydrogenase and the oxidative pentose phosphate cycle protect cells against apoptosis induced by low doses of ionizing radiationQ40877669
Improvement of CHO cell culture medium formulation: simultaneous substitution of glucose and glutamineQ40901774
Improvement of the primary metabolism of cell cultures by introducing a new cytoplasmic pyruvate carboxylase reactionQ40915316
Metabolic shifts by nutrient manipulation in continuous cultures of BHK cellsQ40917436
Regulation of glutaminase activity and glutamine metabolismQ40926056
Elevated glutamate dehydrogenase flux in glucose-deprived hybridoma and myeloma cells: evidence from 1H/15N NMR.Q40963412
The importance of ammonia in mammalian cell cultureQ41009633
Effects of ammonium and lactate on growth and metabolism of a recombinant Chinese hamster ovary cell cultureQ41086618
High viable cell concentration fed-batch cultures of hybridoma cells through on-line nutrient feedingQ41334125
Elementary metabolite units (EMU): a novel framework for modeling isotopic distributionsQ41374953
Influence of ammonium on growth, metabolism, and productivity of a continuous suspension Chinese hamster ovary cell cultureQ41505216
Glucose and glutamine metabolism of a murine B-lymphocyte hybridoma grown in batch cultureQ41516271
Characterization of glutamine metabolism in two related murine hybridomasQ41632841
Effects of ammonia and lactate on hybridoma growth, metabolism, and antibody productionQ41639279
High-level expression of a recombinant antibody from myeloma cells using a glutamine synthetase gene as an amplifiable selectable markerQ41642360
Growth inhibition in animal cell culture. The effect of lactate and ammoniaQ41676171
High level expression of tissue inhibitor of metalloproteinases in Chinese hamster ovary cells using glutamine synthetase gene amplificationQ41730174
Chemical decomposition of glutamine in cell culture media: effect of media type, pH, and serum concentrationQ41743417
Chloroquine and ammonium chloride prevent terminal glycosylation of immunoglobulins in plasma cells without affecting secretionQ41915360
Effects of ammonia on CHO cell growth, erythropoietin production, and glycosylationQ41921972
The effect of ammonia on the O-linked glycosylation of granulocyte colony-stimulating factor produced by chinese hamster ovary cellsQ41932731
Improving glucose and glutamine metabolism of human HEK 293 and Trichoplusia ni insect cells engineered to express a cytosolic pyruvate carboxylase enzymeQ42049015
Dynamic metabolic flux analysis demonstrated on cultures where the limiting substrate is changed from carbon to nitrogen and vice versaQ42430397
A 1H/15N n.m.r. study of nitrogen metabolism in cultured mammalian cellsQ42797179
A detailed metabolic flux analysis of an underdetermined network of CHO cellsQ42876917
Measuring deuterium enrichment of glucose hydrogen atoms by gas chromatography/mass spectrometryQ43138152
Feed development for fed-batch CHO production process by semisteady state analysisQ43219132
Metabolic flux analysis of CHO cells in perfusion culture by metabolite balancing and 2D [13C, 1H] COSY NMR spectroscopyQ43246624
Dynamic model of CHO cell metabolism.Q43711578
Analysis of CHO cells metabolic redistribution in a glutamate-based defined medium in continuous culture.Q43817062
Effects of ammonia and glucosamine on the heterogeneity of erythropoietin glycoforms.Q43873679
Effect of low culture temperature on specific productivity, transcription level, and heterogeneity of erythropoietin in Chinese hamster ovary cellsQ44323806
Effect of culture pH on erythropoietin production by Chinese hamster ovary cells grown in suspension at 32.5 and 37.0 degrees C.Q45206706
Error propagation from prime variables into specific rates and metabolic fluxes for mammalian cells in perfusion culture.Q45955314
Effects of elevated pCO2 and osmolality on growth of CHO cells and production of antibody-fusion protein B1: a case studyQ46498440
Analysis of cellular metabolism of hybridoma cells at distinct physiological statesQ46760109
Ammonia affects the glycosylation patterns of recombinant mouse placental lactogen-I by chinese hamster ovary cells in a pH-dependent mannerQ47866641
A GFP-based method facilitates clonal selection of transfected CHO cells.Q50584043
Accurate assessment of amino acid mass isotopomer distributions for metabolic flux analysis.Q50917980
P433issue1
P304page(s)61-74
P577publication date2011-11-21
P1433published inBiotechnology JournalQ15716480
P1476titleTowards dynamic metabolic flux analysis in CHO cell cultures
P478volume7

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cites work (P2860)
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