Expression and purification of the first nucleotide-binding domain and linker region of human multidrug resistance gene product: comparison of fusions to glutathione S-transferase, thioredoxin and maltose-binding protein

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Expression and purification of the first nucleotide-binding domain and linker region of human multidrug resistance gene product: comparison of fusions to glutathione S-transferase, thioredoxin and maltose-binding protein is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1042/0264-6021:3380077
P932PMC publication ID1220027
P698PubMed publication ID9931301
P5875ResearchGate publication ID13358545

P2093author name stringA F Castro
C Wang
D M Wilkes
G A Altenberg
P2860cites workExpression and functional properties of the second predicted nucleotide binding fold of the cystic fibrosis transmembrane conductance regulator fused to glutathione-S-transferase.Q54613850
Factor Xa cleavage of fusion proteins. Elimination of non-specific cleavage by reversible acylation.Q54714779
Recombinant synthesis, purification, and nucleotide binding characteristics of the first nucleotide binding domain of the cystic fibrosis gene productQ67895667
A method for the determination of inorganic phosphate in the presence of labile organic phosphate and high concentrations of protein: application to lens ATPasesQ69825502
Recombinant N-terminal nucleotide-binding domain from mouse P-glycoprotein. Overexpression, purification, and role of cysteine 430Q71142739
Pas de deux or more: the sulfonylurea receptor and K+ channelsQ72155505
Covalent inhibitors of P-glycoprotein ATPase activityQ72671369
Protein kinase C-mediated phosphorylation of the human multidrug resistance P-glycoprotein regulates cell volume-activated chloride channelsQ28910230
The ATP-binding component of a prokaryotic traffic ATPase is exposed to the periplasmic (external) surfaceQ36064136
A recombinant peptide model of the first nucleotide-binding fold of the cystic fibrosis transmembrane conductance regulator: comparison of wild-type and delta F508 mutant formsQ36279470
Intrinsic anion channel activity of the recombinant first nucleotide binding fold domain of the cystic fibrosis transmembrane regulator proteinQ36846356
Detection of P-glycoprotein isoforms by gene-specific monoclonal antibodiesQ37660701
Nucleotide binding to the hydrophilic C-terminal domain of the transporter associated with antigen processing (TAP).Q38308202
The cystic fibrosis transmembrane conductance regulator. Overexpression, purification, and characterization of wild type and delta F508 mutant forms of the first nucleotide binding fold in fusion with the maltose-binding proteinQ38314343
Domain organization of murine mdr1b P-glycoprotein: the cytoplasmic linker region is a potential dimerization domain.Q38349319
Overexpression of integral membrane proteins for structural studiesQ40370871
The Cystic Fibrosis Transmembrane Conductance RegulatorQ40486029
The nucleotide binding folds of the cystic fibrosis transmembrane conductance regulator are extracellularly accessibleQ41103955
Phosphorylation by cAMP-dependent protein kinase causes a conformational change in the R domain of the cystic fibrosis transmembrane conductance regulatorQ41661209
Multidrug transporters from bacteria to man: similarities in structure and functionQ41688649
Muscarinic stimulation of gallbladder epithelium. III. Antagonism of cAMP-mediated effectsQ42493563
Inhibition of drug transport by genistein in multidrug-resistant cells expressing P-glycoproteinQ42804859
Expression and characterization of the NBD1-R domain region of CFTR: evidence for subunit-subunit interactionsQ46698059
Overexpression and purification of the carboxyl-terminal nucleotide-binding domain from mouse P-glycoprotein. Strategic location of a tryptophan residue.Q50769282
A recombinant polypeptide model of the second nucleotide-binding fold of the cystic fibrosis transmembrane conductance regulator functions as an active ATPase, GTPase and adenylate kinase.Q53967795
Stimulation of CFTR activity by its phosphorylated R domain.Q54558597
Cystic fibrosis transmembrane conductance regulator: the first nucleotide binding fold targets the membrane with retention of its ATP binding function.Q54567502
Expression in Escherichia coli of cytoplasmic portions of the cystic fibrosis transmembrane conductance regulator: apparent bacterial toxicity of peptides containing R-domain sequences.Q54593967
Cloning, overexpression, purification, and characterization of the carboxyl-terminal nucleotide binding domain of P-glycoprotein.Q54610591
P407language of work or nameEnglishQ1860
P921main subjectglutathioneQ116907
multiple drug resistanceQ643839
P304page(s)77-81
P577publication date1999-02-01
P1433published inBiochemical JournalQ864221
P1476titleExpression and purification of the first nucleotide-binding domain and linker region of human multidrug resistance gene product: comparison of fusions to glutathione S-transferase, thioredoxin and maltose-binding protein
P478volume338 ( Pt 1)

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cites work (P2860)
Q34228979A critical review of the methods for cleavage of fusion proteins with thrombin and factor Xa.
Q83451020An effective extracellular protein secretion by an ABC transporter system in Escherichia coli: statistical modeling and optimization of cyclodextrin glucanotransferase secretory production
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Q33663922Expression, purification and structural properties of ABC transporter ABCA4 and its individual domains
Q37038082Foot-and-mouth disease virus-like particles produced by a SUMO fusion protein system in Escherichia coli induce potent protective immune responses in guinea pigs, swine and cattle
Q73089741Fusions to maltose-binding protein: control of folding and solubility in protein purification
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Q44075217S-decyl-glutathione nonspecifically stimulates the ATPase activity of the nucleotide-binding domains of the human multidrug resistance-associated protein, MRP1 (ABCC1).
Q36220537SUMO fusion technology for difficult-to-express proteins
Q33636884Self-assembly of virus-like particles of porcine circovirus type 2 capsid protein expressed from Escherichia coli
Q37486455Several affinity tags commonly used in chromatographic purification
Q42565132Site-directed mutagenesis and expression of the soluble form of the family IIIa cellulose binding domain from the cellulosomal scaffolding protein of Clostridium cellulovorans
Q84872265Syntaxin-1A inhibits KATP channels by interacting with specific conserved motifs within sulfonylurea receptor 2A
Q44525552The N-terminal stem region of bovine and human beta1,4-galactosyltransferase I increases the in vitro folding efficiency of their catalytic domain from inclusion bodies
Q43122669The NBDs that wouldn't die: A cautionary tale of the use of isolated nucleotide binding domains of ABC transporters.
Q44373782The nucleotide-binding domains of P-glycoprotein. Functional symmetry in the isolated domain demonstrated by N-ethylmaleimide labelling
Q35661122The use of Bcl-2 over-expression to stabilize hybridomas specific to the HERG potassium channel

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