Increased titer and reduced lactate accumulation in recombinant retrovirus production through the down-regulation of HIF1 and PDK.

scientific article

Increased titer and reduced lactate accumulation in recombinant retrovirus production through the down-regulation of HIF1 and PDK. is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1002/BIT.25691
P698PubMed publication ID26134455

P50authorMiguel R GuerreiroQ64698599
Ana S. CoroadinhaQ37829098
Paula Marques AlvesQ39849137
Ana F RodriguesQ42408117
P2093author name stringW S Hu
P Fernandes
Y Genzel
A S Formas-Oliveira
A-K Blechert
P2860cites workHypoxia-inducible factor 1 activation by aerobic glycolysis implicates the Warburg effect in carcinogenesisQ43954468
Decreasing lactate level and increasing antibody production in Chinese Hamster Ovary cells (CHO) by reducing the expression of lactate dehydrogenase and pyruvate dehydrogenase kinasesQ44735643
Strategies for fed-batch cultivation of t-PA producing CHO cells: substitution of glucose and glutamine and rational design of culture mediumQ44879306
Glycogen synthase kinase-3 regulates the phosphorylation of severe acute respiratory syndrome coronavirus nucleocapsid protein and viral replicationQ46184735
Understanding the Warburg effect: the metabolic requirements of cell proliferationQ24604760
Significance analysis of microarrays applied to the ionizing radiation responseQ24606608
The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of pathway/genome databasesQ24643451
Short-term cytotoxic effects and long-term instability of RNAi delivered using lentiviral vectorsQ24802565
Modulation of GSK-3β activity in Venezuelan equine encephalitis virus infectionQ28730748
A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screenQ29615061
A weighted average difference method for detecting differentially expressed genes from microarray dataQ31160471
Engineering CHO cell metabolism for growth in galactoseQ34177555
Regulation of pyruvate dehydrogenase complex activity by reversible phosphorylationQ34279293
Nonspecific, concentration-dependent stimulation and repression of mammalian gene expression by small interfering RNAs (siRNAs).Q34365459
Short interfering RNAs can induce unexpected and divergent changes in the levels of untargeted proteins in mammalian cellsQ36603910
Cellular life span and the Warburg effectQ37138183
Novel neuroprotective GSK-3β inhibitor restricts Tat-mediated HIV-1 replicationQ37547409
Cellular targets for improved manufacturing of virus-based biopharmaceuticals in animal cellsQ38275653
Single-step cloning-screening method: a new tool for developing and studying high-titer viral vector producer cells.Q38879455
Adaptation of retrovirus producer cells to serum deprivation: Implications in lipid biosynthesis and vector productionQ39425985
Down-regulation of CD81 tetraspanin in human cells producing retroviral-based particles: tailoring vector composition.Q39527949
Improving baculovirus production at high cell density through manipulation of energy metabolismQ39803264
Retroviral vector production under serum deprivation: The role of lipidsQ39816695
Engineering cell metabolism for high-density cell culture via manipulation of sugar transportQ40100814
Down-regulation of lactate dehydrogenase-A by siRNAs for reduced lactic acid formation of Chinese hamster ovary cells producing thrombopoietinQ40211655
Retrovirus producer cell line metabolism: implications on viral productivity.Q40295162
The use of recombinase mediated cassette exchange in retroviral vector producer cell lines: predictability and efficiency by transgene exchangeQ40306462
Effect of osmotic pressure on the production of retroviral vectors: Enhancement in vector stability.Q40306542
Effect of medium sugar source on the production of retroviral vectors for gene therapy.Q40309177
Impact of dynamic online fed-batch strategies on metabolism, productivity and N-glycosylation quality in CHO cell cultures.Q40461540
Expression of recombinant cytoplasmic yeast pyruvate carboxylase for the improvement of the production of human erythropoietin by recombinant BHK-21 cellsQ40760951
Effects of ammonium and lactate on growth and metabolism of a recombinant Chinese hamster ovary cell cultureQ41086618
Effects of ammonia and lactate on hybridoma growth, metabolism, and antibody productionQ41639279
Improving glucose and glutamine metabolism of human HEK 293 and Trichoplusia ni insect cells engineered to express a cytosolic pyruvate carboxylase enzymeQ42049015
Metabolic pathways recruited in the production of a recombinant enveloped virus: mining targets for process and cell engineeringQ42262872
Measurement of key metabolic enzyme activities in mammalian cells using rapid and sensitive microplate-based assays.Q43047032
P433issue1
P304page(s)150-162
P577publication date2015-09-02
P1433published inBiotechnology and BioengineeringQ4915339
P1476titleIncreased titer and reduced lactate accumulation in recombinant retrovirus production through the down-regulation of HIF1 and PDK
P478volume113

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cites work (P2860)
Q38742446Reduction of ammonia and lactate through the coupling of glutamine synthetase selection and downregulation of lactate dehydrogenase-A in CHO cells
Q59358011Rubella Viruses Shift Cellular Bioenergetics to a More Oxidative and Glycolytic Phenotype with a Strain-Specific Requirement for Glutamine

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