Single-step cloning-screening method: a new tool for developing and studying high-titer viral vector producer cells.

scientific article published on 4 May 2015

Single-step cloning-screening method: a new tool for developing and studying high-titer viral vector producer cells. is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1038/GT.2015.44
P698PubMed publication ID25938191

P50authorHélio TomásQ63961562
Miguel R GuerreiroQ64698599
Ana S. CoroadinhaQ37829098
Paula Marques AlvesQ39849137
Ana F RodriguesQ42408117
P2093author name stringA S Formas-Oliveira
P2860cites workQuantitation of MLV-based retroviral vectors using real-time RT-PCR.Q54716445
Analysis of mutation in human cells by using an Epstein-Barr virus shuttle systemQ24628961
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A third-generation lentivirus vector with a conditional packaging systemQ29616120
Gene therapy finds its nicheQ34163207
A mammalian host-vector system that regulates expression and amplification of transfected genes by temperature inductionQ34563856
Large-scale manufacture and characterization of a lentiviral vector produced for clinical ex vivo gene therapy applicationQ34624921
High-titer packaging cells producing recombinant retroviruses resistant to human serumQ35851698
Large-scale production means for the manufacturing of lentiviral vectorsQ37807436
Production of retroviral vectors: reviewQ37807437
Cellular targets for improved manufacturing of virus-based biopharmaceuticals in animal cellsQ38275653
RD2-MolPack-Chim3, a packaging cell line for stable production of lentiviral vectors for anti-HIV gene therapyQ39139624
Adaptation of retrovirus producer cells to serum deprivation: Implications in lipid biosynthesis and vector productionQ39425985
Lentivirus production is influenced by SV40 large T-antigen and chromosomal integration of the vector in HEK293 cellsQ39546025
Integrated strategy for the production of therapeutic retroviral vectorsQ39636811
Retroviral vector performance in defined chromosomal Loci of modular packaging cell lines.Q39728912
A new PG13-based packaging cell line for stable production of clinical-grade self-inactivating gamma-retroviral vectors using targeted integration.Q39782235
Retroviral vector production under serum deprivation: The role of lipidsQ39816695
Impact of retroviral vector components stoichiometry on packaging cell lines: effects on productivity and vector qualityQ40033275
Engineering of a stable retroviral gene delivery vector by directed evolutionQ40055904
Improving transcriptional termination of self-inactivating gamma-retroviral and lentiviral vectorsQ40150079
A new generation of retroviral producer cells: predictable and stable virus production by Flp-mediated site-specific integration of retroviral vectors.Q40280521
The use of recombinase mediated cassette exchange in retroviral vector producer cell lines: predictability and efficiency by transgene exchangeQ40306462
Rapid screening for high-titer retroviral packaging cell lines using an in situ fluorescence assayQ40724724
HEK293-based production platform for γ-retroviral (self-inactivating) vectors: application for safe and efficient transfer of COL7A1 cDNA.Q41670464
Metabolic pathways recruited in the production of a recombinant enveloped virus: mining targets for process and cell engineeringQ42262872
Evaluation of retroviral vector design in defined chromosomal loci by Flp-mediated cassette replacement.Q42828495
Protein tagging and detection with engineered self-assembling fragments of green fluorescent proteinQ45174130
Rapid titration of retroviral vectors using a β-lactamase protein fragment complementation assayQ45338668
Gene therapy matures in the clinicQ45880394
First gene therapy nears landmark European market authorizationQ45881729
Continuous high-titer HIV-1 vector production.Q52826386
P433issue9
P304page(s)685-695
P577publication date2015-05-04
P1433published inGene TherapyQ15763095
P1476titleSingle-step cloning-screening method: a new tool for developing and studying high-titer viral vector producer cells
P478volume22

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cites work (P2860)
Q38857837Increased titer and reduced lactate accumulation in recombinant retrovirus production through the down-regulation of HIF1 and PDK.
Q54247043LentiPro26: novel stable cell lines for constitutive lentiviral vector production.

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