An active-site guanine participates in glmS ribozyme catalysis in its protonated state

scientific article published on 20 October 2011

An active-site guanine participates in glmS ribozyme catalysis in its protonated state is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1021/JA207426J
P932PMC publication ID3212626
P698PubMed publication ID21936556
P5875ResearchGate publication ID51660334

P50authorJúlia ViladomsQ83139729
P2093author name stringMartha J Fedor
Lincoln G Scott
P2860cites workSelf-sustained replication of an RNA enzymeQ22065854
New RNA motifs suggest an expanded scope for riboswitches in bacterial genetic controlQ24564877
Structural and chemical basis for glucosamine 6-phosphate binding and activation of the glmS ribozymeQ24594199
Riboswitch effectors as protein enzyme cofactorsQ24652676
Structural investigation of the GlmS ribozyme bound to Its catalytic cofactorQ24670513
Mechanism of mRNA destabilization by the glmS ribozymeQ24680823
Core requirements for glmS ribozyme self-cleavage reveal a putative pseudoknot structureQ25257465
Crystal structure of a hairpin ribozyme-inhibitor complex with implications for catalysisQ27631159
Requirement of Helix P2.2 and Nucleotide G1 for Positioning the Cleavage Site and Cofactor of the glmS RibozymeQ27647856
Essential role of an active-site guanine in glmS ribozyme catalysisQ27648983
A 1.9 A crystal structure of the HDV ribozyme precleavage suggests both Lewis acid and general acid mechanisms contribute to phosphodiester cleavageQ27663796
Ribonuclease AQ28201882
Backbone and nucleobase contacts to glucosamine-6-phosphate in the glmS ribozymeQ28240090
Control of gene expression by a natural metabolite-responsive ribozymeQ28251218
Structural basis of glmS ribozyme activation by glucosamine-6-phosphateQ28264810
Catalytic strategies of self-cleaving ribozymesQ28288343
TheglmSribozyme: use of a small molecule coenzyme by a gene-regulatory RNAQ28728642
Small Self-cleaving RibozymesQ28749189
Synthesis of small RNAs using T7 RNA polymeraseQ29615997
Relationship between internucleotide linkage geometry and the stability of RNA.Q34362161
Deciphering the role of glucosamine-6-phosphate in the riboswitch action of glmS ribozymeQ34370143
Glucosamine-6-phosphate synthase--the multi-facets enzyme.Q34666978
The pH dependence of hairpin ribozyme catalysis reflects ionization of an active site adenineQ34978942
Mechanistic considerations for general acid-base catalysis by RNA: revisiting the mechanism of the hairpin ribozymeQ35071324
Ribozyme speed limitsQ35180337
The glmS ribozyme tunes the catalytically critical pK(a) of its coenzyme glucosamine-6-phosphateQ35217480
Analysis of metal ion dependence in glmS ribozyme self-cleavage and coenzyme bindingQ35650668
'In-line attack' conformational effect plays a modest role in an enzyme-catalyzed RNA cleavage: a free energy simulation studyQ35891634
Nucleobase catalysis in ribozyme mechanismQ36578719
Synthesis of RNA probes by the direct in vitro transcription of PCR-generated DNA templatesQ36784840
Comparative enzymology and structural biology of RNA self-cleavageQ37470678
1 Phosphoribosylprophosphate synthetase (ribose-5-phosphate pyrophosphokinase) from Salmonella typhimuriumQ40172913
A pseudoknot in the 3' non-core region of the glmS ribozyme enhances self-cleavage activityQ40346444
Tertiary structure stabilization promotes hairpin ribozyme ligationQ41686849
Preparation of specifically 2H- and 13C-labeled ribonucleotidesQ41738202
Protonation states of the key active site residues and structural dynamics of the glmS riboswitch as revealed by molecular dynamicsQ41809931
An expanded collection and refined consensus model of glmS ribozymesQ42052505
Trans-acting glmS catalytic riboswitch: locked and loadedQ42094848
Rapid steps in the glmS ribozyme catalytic pathway: cation and ligand requirementsQ42726378
glmS Riboswitch binding to the glucosamine-6-phosphate α-anomer shifts the pKa toward neutralityQ42869072
Direct measurement of the ionization state of an essential guanine in the hairpin ribozymeQ43143817
Characteristics of the glmS ribozyme suggest only structural roles for divalent metal ions.Q43256741
8-Aza-2'-deoxyguanosine: base pairing, mismatch discrimination and nucleobase anion fluorescence sensing in single-stranded and duplex DNA.Q43292707
Catalytic roles for proton transfer and protonation in ribozymesQ43722704
Model substrates for an RNA enzymeQ45167887
A rate-limiting conformational step in the catalytic pathway of the glmS ribozyme.Q45902451
8-Azaguanine reporter of purine ionization states in structured RNAsQ46411889
Ligand requirements for glmS ribozyme self-cleavageQ46812332
Ribokinase from Escherichia coli K12. Nucleotide sequence and overexpression of the rbsK gene and purification of ribokinaseQ47598146
Fluorescence emission properties of 8-azapurines and their nucleosides, and application to the kinetics of the reverse synthetic reaction of purine nucleoside phosphorylase.Q50539406
Evidence for the role of solvated metal hydroxide in the hammerhead cleavage mechanismQ72609634
Integration of PCR fragments at any specific site within cloning vectors without the use of restriction enzymes and DNA ligaseQ74238681
Two-stage polymerase chain reaction protocol allowing introduction of multiple mutations, deletions, and insertions, using QuikChange site-directed mutagenesisQ77426888
P4510describes a project that usesImageQuantQ112270642
P433issue45
P407language of work or nameEnglishQ1860
P921main subjectribozymeQ205858
P304page(s)18388-18396
P577publication date2011-10-20
P1433published inJournal of the American Chemical SocietyQ898902
P1476titleAn active-site guanine participates in glmS ribozyme catalysis in its protonated state
P478volume133

Reverse relations

cites work (P2860)
Q382400468-Azapurines as isosteric purine fluorescent probes for nucleic acid and enzymatic research.
Q27680143An in vitro evolved glmS ribozyme has the wild-type fold but loses coenzyme dependence
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Q36997163Ribozyme Catalysis with a Twist: Active State of the Twister Ribozyme in Solution Predicted from Molecular Simulation
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Q35020704Role of the active site guanine in the glmS ribozyme self-cleavage mechanism: quantum mechanical/molecular mechanical free energy simulations
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Q51139880The GlcN6P cofactor plays multiple catalytic roles in the glmS ribozyme.
Q28278331The glmS ribozyme cofactor is a general acid-base catalyst
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