Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products

scientific article published on March 1991

Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products is …
instance of (P31):
scholarly articleQ13442814

External links are
P356DOI10.1093/NAR/19.5.1154
P932PMC publication ID333800
P698PubMed publication ID2020552
P5875ResearchGate publication ID21134563

P50authorFrancis CollinsQ336658
P2093author name stringDrumm M
Marchuk D
Saulino A
P2860cites workDirect cloning and sequence analysis of enzymatically amplified genomic sequencesQ68732880
Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemiaQ27861076
Ligation-independent cloning of PCR products (LIC-PCR)Q29618962
PCR-induced (ligase-free) subcloning: a rapid reliable method to subclone polymerase chain reaction (PCR) productsQ35836311
Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerasesQ36070698
Using the polymerase chain reaction to modify expression plasmids for epitope mapping.Q40449124
A simple method for site-directed mutagenesis using the polymerase chain reactionQ40452884
Restriction endonuclease cleavage at the termini of PCR productsQ41791259
DMA mutagenesis and recombinationQ59006170
P433issue5
P304page(s)1154
P577publication date1991-03-01
P1433published inNucleic Acids ResearchQ135122
P1476titleConstruction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products
P478volume19

Reverse relations

cites work (P2860)
Q24678481'VIT1', a novel gene associated with vitiligo
Q480387672,4-Dichlorophenoxybutyric acid-resistant mutants of Arabidopsis have defects in glyoxysomal fatty acid beta-oxidation
Q367608453-Ketoacyl-acyl carrier protein synthase III from spinach (Spinacia oleracea) is not similar to other condensing enzymes of fatty acid synthase
Q546657348 Efficient complementary dna amplification and expression using polymerase chain reaction technology
Q43697729A Kunitz trypsin inhibitor gene family from trembling aspen (Populus tremuloides Michx.): cloning, functional expression, and induction by wounding and herbivory
Q57291904A Musashi Splice Variant and its Interaction Partners Influence Temperature Acclimation in Chlamydomonas
Q73539585A T-extended vector using a green fluorescent protein as an indicator
Q30833804A bone sialoprotein-binding protein from Staphylococcus aureus: a member of the staphylococcal Sdr family
Q33367082A cdc2 homologue and closely related processed retropseudogenes from Norway spruce
Q48047886A class IV chitinase is highly expressed in grape berries during ripening
Q36659681A conserved alternative splice in the von Recklinghausen neurofibromatosis (NF1) gene produces two neurofibromin isoforms, both of which have GTPase-activating protein activity
Q48037382A dynein light chain of sea urchin sperm flagella is a homolog of mouse Tctex 1, which is encoded by a gene of the t complex sterility locus
Q40544761A high proportion of chromosome 21 promoter polymorphisms influence transcriptional activity
Q45968748A highly efficient molecular cloning platform that utilises a small bacterial toxin gene.
Q34668102A method for analyzing the qualitative and quantitative aspects of gene expression: a transcriptional profile revealed for HeLa cells
Q24316980A molecular basis for cardiac arrhythmia: HERG mutations cause long QT syndrome
Q36235903A mutation in glyceraldehyde 3-phosphate dehydrogenase alters endocytosis in CHO cells
Q36059319A mutation within intron 3 of the Pax-3 gene produces aberrantly spliced mRNA transcripts in the splotch (Sp) mouse mutant
Q39680415A new modified ortho cleavage pathway of 3-chlorocatechol degradation by Rhodococcus opacus 1CP: genetic and biochemical evidence
Q34150118A novel binary T-vector with the GFP reporter gene for promoter characterization
Q48386648A novel mitochondrial gene order in the crinoid echinoderm Florometra serratissima
Q36200883A novel process of viral vector barcoding and library preparation enables high-diversity library generation and recombination-free paired-end sequencing
Q33303409A novel technique for efficient construction of large scFv libraries
Q45762609A polymerase chain reaction-based assay for diagnosing varicella-zoster virus retinitis in patients with acquired immunodeficiency syndrome
Q41106244A recombinant GM-CSF-PE40 ligand toxin is functionally active but not cytotoxic to cells
Q41331703A role for FGF-8 in the initiation and maintenance of vertebrate limb bud outgrowth
Q33889824A role for trigger factor and an rgg-like regulator in the transcription, secretion and processing of the cysteine proteinase of Streptococcus pyogenes
Q35198666A simple method for quantification of uncultured microorganisms in the environment based on in vitro transcription of 16S rRNA.
Q30795541A starch-branching enzyme gene in wheat produces alternatively spliced transcripts
Q41715586A strategy for rapid cDNA cloning from double-stranded RNA templates isolated from plants infected with RNA viruses by using Taq DNA polymerase
Q31118861A survey of the composition and diversity of bacterial populations in bleached kraft pulp-mill wastewater secondary treatment systems
Q48064451A thiol protease and an anionic peroxidase are induced by lowering cytokinins during callus growth in Petunia.
Q36231897A third tal-1 promoter is specifically used in human T cell leukemias
Q67528341A universal method for the direct cloning of PCR amplified nucleic acid
Q47915310A versatile zero background T-vector system for gene cloning and functional genomics
Q28294936AKAP350, a multiply spliced protein kinase A-anchoring protein associated with centrosomes
Q41111222Activation of fibroblast growth factor 8 gene expression in human embryonal carcinoma cells
Q36827136Activation of the mouse mdr3 gene by insertion of retroviruses in multidrug-resistant P388 tumor cells
Q28755532Active miniature transposons from a plant genome and its nonrecombining Y chromosome
Q30168524Additional complexity on human chromosome 15q: identification of a set of newly recognized duplicons (LCR15) on 15q11-q13, 15q24, and 15q26.
Q77425102Agonist-induced down-regulation of the beta2-adrenoceptor and its mRNA in human mononuclear leukocytes
Q24538391Ahi-1, a novel gene encoding a modular protein with WD40-repeat and SH3 domains, is targeted by the Ahi-1 and Mis-2 provirus integrations
Q43544081Alanine scan mutagenesis of the switch I domain of the Caulobacter crescentus CgtA protein reveals critical amino acids required for in vivo function
Q71743161Alternative splicing of DNA polymerase beta mRNA is not tumor-specific
Q48075404Alternative splicing of ED-A and ED-B sequences of fibronectin pre-mRNA differs in chondrocytes from different cartilaginous tissues and can be modulated by biological factors
Q45628504An ELISA for putative neutralizing antibodies to hepatitis E virus detects antibodies to genotypes 1, 2, 3, and 4.
Q36564015An alternative splicing event in the Pax-3 paired domain identifies the linker region as a key determinant of paired domain DNA-binding activity.
Q61450248An alternative to blunt-end ligation for cloning DNA fragments with incompatible ends
Q40410151An efficient method to generate phosphatase insensitive 3' labelled DNA probes using Taq polymerase
Q41816342An efficient method to prepare PCR cloning vectors
Q33228330An essential gene for fruiting body initiation in the basidiomycete Coprinopsis cinerea is homologous to bacterial cyclopropane fatty acid synthase genes
Q41542575An exon‐trapping system with a newly constructed trapping vector pEXT2; its application to the proximal region of the human chromosome 21 long arm
Q48049470An interstitial telomere array proximal to the distal telomere of mouse chromosome 13.
Q43040748Analysing the sequence diversity of the 5' NC-region of HCV-isolates found in southern Germany
Q30453916Analysis of lipopolysaccharide-response genes in B-lineage cells demonstrates that they can have differentiation stage-restricted expression and contain SH2 domains.
Q46781958Analysis of mammalian MUC1 genes reveals potential functionally important domains
Q72556490Analysis of ras mutations in human melanocytic lesions: activation of the ras gene seems to be associated with the nodular type of human malignant melanoma
Q44170114Analysis of the Anopheles gambiae genome using RAPD markers
Q34809534Analysis of the imperfect octamer-containing human immunoglobulin VH6 gene promoter
Q52131141Anterior expression of the caudal homologue cCdx-B activates a posterior genetic program in avian embryos.
Q38353232Antigen binding and cytotoxic properties of a recombinant immunotoxin incorporating the lytic peptide, melittin
Q47881174Antigenic and genetic characterization of a putative hybrid transferrin-binding protein B from Neisseria meningitidis
Q28143773Antizyme2 is a negative regulator of ornithine decarboxylase and polyamine transport
Q54651085Assembly of eukaryotic class III (N-out, C-in) membrane proteins into the Escherichia coli cytoplasmic membrane.
Q28633912Association of the high-affinity receptor for IgG (Fc gamma RI) with the gamma subunit of the IgE receptor
Q35194956Association of transforming growth-factor alpha gene polymorphisms with nonsyndromic cleft palate only (CPO).
Q34809613Bidirectional transcription from the human immunoglobulin VH6 gene promoter
Q42055759Binding of Bacillus thuringiensis delta-endotoxins Cry1Ac and Cry1Ba to a 120-kDa aminopeptidase-N of Epiphyas postvittana purified from both brush border membrane vesicles and baculovirus-infected Sf9 cells.
Q24679332Biochemical and functional analysis of the Borna disease virus G protein
Q44954093Biochemical and molecular characterization of a ring fission dioxygenase with the ability to oxidize (substituted) salicylate(s) from Pseudaminobacter salicylatoxidans
Q41010195Blood pressure regulates platelet-derived growth factor A-chain gene expression in vascular smooth muscle cells in vivo. An autocrine mechanism promoting hypertensive vascular hypertrophy
Q36642180Bovine leukemia virus, an animal model for the study of intrastrain variability
Q59055972Calcium channel β-subunit binds to a conserved motif in the I–II cytoplasmic linker of the α1-subunit
Q50111245Calcium-evoked insulin release from insulinoma cells is mediated via calcium-sensing receptor
Q81579460Case report of systemic coccidiosis in a radiated tortoise (Geochelone radiata)
Q36140262Casposon integration shows strong target site preference and recapitulates protospacer integration by CRISPR-Cas systems
Q34006392Cell vacuolation caused by Vibrio cholerae hemolysin
Q28183233Centractin is an actin homologue associated with the centrosome
Q52204497Changes in the expression of fibroblast growth factor receptors mark distinct stages of chondrogenesis in vitro and during chick limb skeletal patterning.
Q48277411Changes of carp FoF1-ATPase in association with temperature acclimation
Q24628850Characterisation of a novel minisatellite that provides multiple splice donor sites in an interferon-induced transcript
Q47884315Characterisation of the proximal regulatory domain of the Echinococcus granulosus homeodomain-containing gene EgHbx1.
Q47772275Characterization and molecular evolution of a vertebrate hyaluronan synthase gene family
Q40042199Characterization of a Borna disease virus glycoprotein, gp18.
Q30956942Characterization of a Xenopus laevis CXC chemokine receptor 4: implications for hematopoietic cell development in the vertebrate embryo
Q36517929Characterization of a hepatoma mRNA transcribed from a third promoter of a 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-encoding gene and controlled by ets oncogene-related products
Q39564777Characterization of a protocatechuate catabolic gene cluster from Rhodococcus opacus 1CP: evidence for a merged enzyme with 4-carboxymuconolactone-decarboxylating and 3-oxoadipate enol-lactone-hydrolyzing activity
Q47955528Characterization of an alternatively spliced isoform of rat vesicle associated membrane protein-2 (VAMP-2).
Q39844012Characterization of catechol catabolic genes from Rhodococcus erythropolis 1CP
Q95294892Characterization of mutants with alterations of the phosphorylation site in the D2 photosystem II polypeptide of chlamydomonas reinhardtii
Q42110249Characterization of qnrB-like genes in Citrobacter species of the American Type Culture Collection
Q31987643Characterization of the Huntington's disease (HD) gene homologue in the zebrafish Danio rerio.
Q41229003Characterization of the Xenopus rhodopsin gene
Q36868254Characterization of the bovine C alpha gene
Q33994819Characterization of the genes for two protocatechuate 3, 4-dioxygenases from the 4-sulfocatechol-degrading bacterium Agrobacterium radiobacter strain S2.
Q28572911Characterization of the rat cytoplasmic C1-tetrahydrofolate synthase gene and analysis of its expression in liver regeneration and fetal development
Q48079736Chitinolytic bacteria in the intestinal tract of Japanese coastal fishes
Q36685052Cloning and characterization of a cDNA encoding the baboon tissue inhibitor of matrix metalloproteinase-1 (TIMP-1)
Q24320167Cloning and characterization of a novel gene (TM7SF1) encoding a putative seven-pass transmembrane protein that is upregulated during kidney development
Q48085332Cloning and characterization of a tomato GTPase-like gene related to yeast and Arabidopsis genes involved in vesicular transport
Q47860051Cloning and characterization of amphibian cold inducible RNA-binding protein
Q81366896Cloning and characterization of ethanol-regulated esterase genes in Acetobacter pasteurianus
Q42796657Cloning and expression of the bovine intestinal alkaline phosphatase gene: biochemical characterization of the recombinant enzyme
Q55921008Cloning and functional characterization of the 5′ flanking region of the human mitochondrial malic enzyme gene
Q48065380Cloning and functional expression of a plant voltage-dependent chloride channel
Q28510779Cloning and functional expression of the mouse homologue of p47phox
Q39650079Cloning and heterologous expression of an enantioselective amidase from Rhodococcus erythropolis strain MP50.
Q35684766Cloning and sequence analysis of the rat augmenter of liver regeneration (ALR) gene: expression of biologically active recombinant ALR and demonstration of tissue distribution
Q30993012Cloning and sequencing of the cDNA encoding rice elongation factor 1 beta'.
Q72322672Cloning of PCR fragments with a modified M13mp18 T-vector
Q32077516Cloning of a cDNA from Arabidopsis thaliana homologous to the human XPB gene.
Q47798497Cloning of cDNA encoding a novel isoform (type IV) of peptidylarginine deiminase from rat epidermis
Q48029797Cloning of novel ABC transporter genes
Q53881985Cloning of porcine IGF1 receptor cDNA and detection of sequence polymorphisms using RT-PCR.
Q48137601Cloning of the cta operon from alkaliphilic Bacillus firmus OF4 and characterization of the pH-regulated cytochrome caa3 oxidase it encodes
Q43674774Cloning of the genes for a 4-sulphocatechol-oxidizing protocatechuate 3,4-dioxygenase from Hydrogenophaga intermedia S1 and identification of the amino acid residues responsible for the ability to convert 4-sulphocatechol
Q34965915Cloning of two novel forms of human acidic fibroblast growth factor (aFGF) mRNA
Q35613794Cloning, expression, and isolation of the mannitol transport protein from the thermophilic bacterium Bacillus stearothermophilus
Q40150163Cloning, expression, and promoter analysis of hepatic lipase derived from human hyperplastic adrenals: evidence for alternative mRNA splicing
Q28378939Cloning, sequencing and expression of rat liver 3-phosphoglycerate dehydrogenase
Q24319933Cloning, sequencing and functional expression of a cDNA encoding a NADP-dependent malic enzyme from human liver
Q39485996Cloning, sequencing, and expression in Escherichia coli of the new gene encoding beta-1,3-xylanase from a marine bacterium, Vibrio sp. strain XY-214.
Q34433229Co-amplification explains linkage disequilibrium of two mosquito esterase genes in insecticide-resistant Culex quinquefasciatus
Q40407603Coconut cadang-cadang viroid (CCCVd) mutants associated with severe disease vary in both the pathogenicity domain and the central conserved region.
Q39864781Combination of cell culture and quantitative PCR for screening of drugs against Cryptosporidium parvum
Q40975598Comparative analysis of three murine G-protein coupled receptors activated by sphingosine-1-phosphate.
Q39723156Comparative mutagenicities of N6-methoxy-2,6-diaminopurine and N6-methoxyaminopurine 2'-deoxyribonucleosides and their 5'-triphosphates
Q36560710Comparison of targeted-gene replacement frequencies in Drosophila melanogaster at the forked and white loci
Q47955290Complete mitochondrial genome DNA sequence for two ophiuroids and a holothuroid: the utility of protein gene sequence and gene maps in the analyses of deep deuterostome phylogeny
Q42612614Complete nucleotide sequence of an isolate of the nepovirus raspberry ringspot virus from grapevine
Q30718051Complete nucleotide sequence, genome organization and phylogenic analysis of the canine calicivirus
Q42648427Complete nucleotide sequences of the RNAs 2 of German isolates of grapevine fanleaf and Arabis mosaic nepoviruses
Q38887893Concerted evolution at a multicopy locus in the protozoan parasite Theileria parva: extreme divergence of potential protein-coding sequences
Q33836953Conditional transgenic system for mouse aurora a kinase: degradation by the ubiquitin proteasome pathway controls the level of the transgenic protein
Q34389720Congenital insensitivity to pain with anhidrosis: novel mutations in the TRKA (NTRK1) gene encoding a high-affinity receptor for nerve growth factor
Q43049368Conservation of hepatitis C virus 5' untranslated sequences in hepatocellular carcinoma and the surrounding liver
Q38888379Conservation of the sporozoite p67 vaccine antigen in cattle-derived Theileria parva stocks with different cross-immunity profiles
Q46825205Cost-effective method of DNA extraction from taeniid eggs.
Q50757596Cryptic splice site activation during RNA processing of MLL/AF4 chimeric transcripts in infants with t(4;11) positive ALL.
Q46384109Crystal structure of the hydroxyquinol 1,2-dioxygenase from Nocardioides simplex 3E, a key enzyme involved in polychlorinated aromatics biodegradation
Q36874843Cuphea wrightii thioesterases have unexpected broad specificities on saturated fatty acids
Q39538631Cyanobacterial sulfide-quinone reductase: cloning and heterologous expression
Q47967220Cytokinin oxidase from Zea mays: purification, cDNA cloning and expression in moss protoplasts
Q40519958DISEC-TRISEC: di- and trinucleotide-sticky-end cloning of PCR-amplified DNA
Q27469515DNA immunization with Japanese encephalitis virus nonstructural protein NS1 elicits protective immunity in mice
Q42049376DNA polymerase gene sequences indicate western and forest tent caterpillar viruses form a new taxonomic group within baculoviruses
Q39931009DNA sequence divergence among derivatives of Escherichia coli K-12 detected by arbitrary primer PCR (random amplified polymorphic DNA) fingerprinting
Q39489184DSBCapture: in situ capture and sequencing of DNA breaks.
Q73043596Decreased expression and rare somatic mutation of the CIP1/WAF1 gene in human hepatocellular carcinoma
Q45752637Deletions in the hepatitis B virus core gene may influence the clinical outcome in hepatitis B e antigen-positive asymptomatic healthy carriers
Q40901944Detection and characterization of conjugative degradative plasmids in xenobiotic-degrading Sphingomonas strains
Q43998351Detection and differentiation of serologically cross-reacting tobamoviruses of economical importance by RT-PCR and RT-PCR-RFLP.
Q40175989Detection and nucleotide sequence analysis of the speC gene in Swedish clinical group A streptococcal isolates
Q42529017Detection of HPV DNA in trichilemmomas by polymerase chain reaction
Q33782826Detection of a novel bovine lymphotropic herpesvirus.
Q24322303Detection of ebaf, a novel human gene of the transforming growth factor beta superfamily association of gene expression with endometrial bleeding
Q45756667Detection of respiratory syncytial virus RNA in blood of neonates by polymerase chain reaction
Q54665637Developing antibodies to synthetic peptides based on comparative DNA sequencing of multigene families.
Q42110408Development of a dual-expression vector facilitated with selection-free PCR recombination cloning strategy
Q47584068Development of a gene-trap vector with a highly sensitive fluorescent protein reporter system for expression profiling
Q74272324Development of screening systems for drugs against human papillomavirus-associated cervical cancer: based on E6-E6AP binding
Q48038716Development of sequence characterized DNA markers linked to a dominant verticillium wilt resistance gene in tomato
Q48065574Developmental expression of a tandemly repeated, proline-and glutamine-rich amino acid motif on hyphal surfaces on Candida albicans
Q52175324Developmental expression of the four plasma membrane calcium ATPase (Pmca) genes in the mouse.
Q52141074Developmental mosaicism may explain spontaneous reappearance of the Axin(Fu) mutation in mice.
Q48045746Differential expression of distinct members of Rho family GTP-binding proteins during neuronal development: identification of Rac1B, a new neural-specific member of the family
Q57120343Diversity and origins of endophytic fungal symbionts of the North American grass Festuca arizonica
Q40564744Diversity of vertebrate splicing factor U2AF35: identification of alternatively spliced U2AF1 mRNAS.
Q40790307Down modulation of IL-18 expression by human papillomavirus type 16 E6 oncogene via binding to IL-18.
Q38949191Down-regulation of GPR137 expression inhibits proliferation of colon cancer cells
Q35188701Drosophila P-element transposase is a novel site-specific endonuclease
Q31982767Drosophila ferritin mRNA: alternative RNA splicing regulates the presence of the iron-responsive element
Q40405300Efficient cloning of fragments of the polymerase chain reaction directly into the single stranded bacteriophage M13mp18.
Q52172588Efficient integration of short interspersed element-flanked foreign DNA via homologous recombination.
Q52143200Electrical activity and postsynapse formation in adult muscle: gamma-AChRs are not required.
Q40523221Elimination of endogenous aberrant kappa chain transcripts from sp2/0-derived hybridoma cells by specific ribozyme cleavage: utility in genetic therapy of HIV-1 infections
Q40990022Elucidation of the genetic basis of the antigen presentation defects in the mutant cell line .220 reveals polymorphism and alternative splicing of the tapasin gene
Q41383380Endogenous antigen presentation by autoantigen-transfected Epstein-Barr virus-lymphoblastoid cells: T cell receptor N-region hydrophobicity relates to thyroid antigen recognition
Q24306306Enzymatic synthesis in vitro of the disulfated disaccharide unit of corneal keratan sulfate
Q41260375Epitope-labeled soluble human interleukin-5 (IL-5) receptors. Affinity cross-link labeling, IL-5 binding, and biological activity
Q35586187Epstein-Barr virus (EBV) subtype in EBV related oral squamous cell carcinoma in Okinawa, a subtropical island in southern Japan, compared with Kitakyushu and Kumamoto in mainland Japan
Q36543781Evaluation of a quantitative plasma PCR plate assay for detecting cytomegalovirus infection in marrow transplant recipients.
Q35888800Evidence for translation of the Borna disease virus G protein by leaky ribosomal scanning and ribosomal reinitiation.
Q39444301Evidence that protein binding specifies sites of DNA demethylation
Q28776035Evolution of the leucine gene cluster in Buchnera aphidicola: insights from chromosomal versions of the cluster
Q38311082Evolutionary appearance of genes encoding proteins associated with box H/ACA snoRNAs: cbf5p in Euglena gracilis, an early diverging eukaryote, and candidate Gar1p and Nop10p homologs in archaebacteria
Q39564783Evolutionary relationship between chlorocatechol catabolic enzymes from Rhodococcus opacus 1CP and their counterparts in proteobacteria: sequence divergence and functional convergence.
Q35757771Evolutionary strata on the X chromosomes of the dioecious plant Silene latifolia: evidence from new sex-linked genes
Q31027429Exhaustive identification of interaction domains using a high-throughput method based on two-hybrid screening and PCR-convergence: molecular dissection of a kinetochore subunit Spc34p.
Q47949277Expansins are conserved in conifers and expressed in hypocotyls in response to exogenous auxin
Q34541874Exponential megapriming PCR (EMP) cloning--seamless DNA insertion into any target plasmid without sequence constraints.
Q36797422Expression in Escherichia coli, purification and characterization of heparinase I from Flavobacterium heparinum
Q37469551Expression of functional Plasmodium falciparum enzymes using a wheat germ cell-free system
Q77355564Expression of human macrophage metalloelastase gene in hepatocellular carcinoma: correlation with angiostatin generation and its clinical significance
Q40916385Expression of interleukin-16 by human epithelial cells. Inhibition by dexamethasone.
Q73152473Expression of neurotrophic factors in cultured human retinal pigment epithelial cells
Q42515247Expression of transforming growth factor beta in the embryonic avian lens coincides with the presence of mitochondria.
Q28142385Expression pattern and gene characterization of asporin. a newly discovered member of the leucine-rich repeat protein family
Q42070807Expression, characterization and purification of soluble G-protein beta gamma dimers composed of defined subunits in baculovirus-infected insect cells
Q28728161Extensive pyrosequencing reveals frequent intra-genomic variations of internal transcribed spacer regions of nuclear ribosomal DNA
Q53864068Far different levels of gene expression provided by an oriented cloning system inBacillus subtilisandEscherichia coli
Q31033460FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method
Q35838014Fgf-8, activated by proviral insertion, cooperates with the Wnt-1 transgene in murine mammary tumorigenesis.
Q56341582Fifty-nine microsatellite markers for hybrid classification studies involving endemic Florida Mottled Duck (Anas fulvigula fulvigula) and invasive Mallards (A. platyrhynchos)
Q37043982Fluorescent proteins in zebrafish cell and developmental biology
Q39679110Functional characterization and regulation of gadX, a gene encoding an AraC/XylS-like transcriptional activator of the Escherichia coli glutamic acid decarboxylase system
Q39585663Functional organization and insertion specificity of IS607, a chimeric element of Helicobacter pylori
Q41576170Fusion of the erythropoietin receptor and the Friend spleen focus-forming virus gp55 glycoprotein transforms a factor-dependent hematopoietic cell line
Q28214769Gankyrin is an ankyrin-repeat oncoprotein that interacts with CDK4 kinase and the S6 ATPase of the 26 S proteasome
Q30704478Gene identification by exon amplification
Q43246866Gene silencing of HIV chemokine receptors using ribozymes and single-stranded antisense RNA.
Q36869131Gene structure and functional analysis of the human Na+/phosphate co-transporter
Q35777707General method for direct cloning of DNA fragments generated by the polymerase chain reaction
Q50734070Generation of a mouse with conditionally activated signaling through the BMP receptor, ALK2.
Q42643423Genetic analysis of Borrelia burgdorferi sensu lato in Korea using genomic hybridization and 16S rRNA gene sequence determination
Q27469408Genetic and serological evidence for multiple instances of unrecognized transmission of hepatitis C virus in hemodialysis units
Q35192536Genetic diversity in marine algal virus communities as revealed by sequence analysis of DNA polymerase genes.
Q33966245Genetic map of randomly amplified DNA polymorphisms closely linked to the mating type locus of Tetrahymena thermophila.
Q42679686Genetic organization and sequence analysis of the hypha-specific cell wall protein gene HWP1 of Candida albicans
Q42640891Genome analysis of the 3'-terminal part of the little cherry disease associated dsRNA reveals a monopartite clostero-like virus
Q28181428Genomic structure, evolutionary conservation and aniridia mutations in the human PAX6 gene
Q33277339Genotypic microbial community profiling: a critical technical review
Q24643379H36-alpha 7 is a novel integrin alpha chain that is developmentally regulated during skeletal myogenesis
Q48333785HCF164 encodes a thioredoxin-like protein involved in the biogenesis of the cytochrome b(6)f complex in Arabidopsis
Q37170340Hagfish humoral defense protein exhibits structural and functional homology with mammalian complement components
Q41743928Hallmarks of hepatitis C virus in equine hepacivirus
Q24681140Hepatic cholesterol metabolism and resistance to dietary cholesterol in LXRbeta-deficient mice
Q42480160Hepatic expression of acute-phase protein genes during carcinogenesis induced by peroxisome proliferators
Q34599646Hetero-stagger cloning: efficient and rapid cloning of PCR products
Q54149405Heterologous expression of an active rat regulatory protein of glucokinase.
Q36233850High expression of 92-kD type IV collagenase (gelatinase B) in the osteoclast lineage during mouse development
Q33743392High-efficiency scarless genetic modification in Escherichia coli by using lambda red recombination and I-SceI cleavage
Q36556609High-frequency illegitimate integration of transfected DNA at preintegrated target sites in a mammalian genome
Q39750626Homologous npdGI Genes in 2,4-Dinitrophenol- and 4-Nitrophenol-Degrading Rhodococcus spp
Q48083944Hoxa-2 expression in normal and transposed rhombomeres: independent regulation in the neural tube and neural crest
Q24321732Human and Xenopus cDNAs encoding budding yeast Cdc7-related kinases: in vitro phosphorylation of MCM subunits by a putative human homologue of Cdc7
Q39756511Human cytomegalovirus tegument proteins ppUL82 (pp71) and ppUL35 interact and cooperatively activate the major immediate-early enhancer
Q53393697Human delta Np73 regulates a dominant negative feedback loop for TAp73 and p53.
Q24630121Human fibroblast growth factor 1 gene expression in vascular smooth muscle cells is modulated via an alternate promoter in response to serum and phorbol ester
Q35587639Human herpesvirus 8 (HHV8) sequence variations in HHV8 related tumours in Okinawa, a subtropical island in southern Japan
Q28776505Hypermutation in derepressed operons of Escherichia coli K12
Q34462466Identification and characterization of a new class of human immunodeficiency virus type 1 recombinants comprised of two circulating recombinant forms, CRF07_BC and CRF08_BC, in China
Q48080540Identification and characterization of the gene causing type 1 spinocerebellar ataxia
Q24313272Identification and overlapping expression of multiple unconventional myosin genes in vertebrate cell types
Q36870504Identification of Neuronal Isozyme Specific Residues by Comparison of Goldfish Aldolase C to Other Aldolases
Q54191296Identification of a codominant amplified polymorphic DNA marker linked to the verticillium wilt resistance gene in tomato.
Q48076659Identification of a highly polymorphic length variant in the 3'UTR of NRAMP1
Q24290727Identification of a novel exon in apolipoprotein E receptor 2 leading to alternatively spliced mRNAs found in cells of the vascular wall but not in neuronal tissue
Q42232506Identification of a novel self-sufficient styrene monooxygenase from Rhodococcus opacus 1CP.
Q36716034Identification of functional domains of interleukin-3 by construction of primate interspecies chimera
Q45776699Identification of human T cell leukemia virus type IIb infection in the Wayu, an aboriginal population of Colombia.
Q24313535Identification of natural resistance-associated macrophage protein in peripheral blood lymphocytes
Q24314240Identification of polymorphisms and sequence variants in the human homologue of the mouse natural resistance-associated macrophage protein gene
Q31149438Identification of region-specific yeast artificial chromosomes using pools of Alu element-mediated polymerase chain reaction probes labeled via linear amplification
Q36636211Identification of signal sequences that control transcription of borna disease virus, a nonsegmented, negative-strand RNA virus
Q33329036Identification of soluble protein fragments by gene fragmentation and genetic selection.
Q36836312Identification of the dipteran Leu-callatostatin peptide family: characterisation of the prohormone gene from Calliphora vomitoria and Lucilia cuprina
Q28283017Identification of the p53 family-responsive element in the promoter region of the tumor suppressor gene hypermethylated in cancer 1
Q24674123Identification, characterization and cloning of myr 1, a mammalian myosin-I
Q40061100Immune TB Antibody Phage Display Library as a Tool To Study B Cell Immunity in TB Infections.
Q36292075In oesophageal squamous cell carcinoma vascular endothelial growth factor is associated with p53 mutation, advanced stage and poor prognosis
Q40412972In vivo cloning of PCR products in E. coli
Q40533323In-cell PCR from mRNA: amplifying and linking the rearranged immunoglobulin heavy and light chain V-genes within single cells
Q46006259Inactivation of two mouse alkaline phosphatase genes and establishment of a model of infantile hypophosphatasia.
Q48076178Induction of CL100 protein tyrosine phosphatase following transient forebrain ischemia in the rat brain
Q38341073Induction of PBP74/mortalin/Grp75, a member of the hsp70 family, by low doses of ionizing radiation: a possible role in induced radioresistance
Q40334243Induction of nitric oxide synthase by cyclic AMP in rat vascular smooth muscle cells
Q43553510Infection density of Wolbachia and incompatibility level in two planthopper species, Laodelphax striatellus and Sogatella furcifera
Q74012713Influence of macrophage-derived apolipoprotein E on plasma lipoprotein distribution of apolipoprotein A-I in apolipoprotein E-deficient mice
Q40820328Inhibition of antigen transport by expression of infected cell peptide 47 (ICP47) prevents cell surface expression of HLA in choriocarcinoma cell lines
Q33967790Integrated genetic map of Anopheles gambiae: use of RAPD polymorphisms for genetic, cytogenetic and STS landmarks
Q39877349Intracellular expression of single-chain variable fragments to inhibit early stages of the viral life cycle by targeting human immunodeficiency virus type 1 integrase.
Q34193354Intramembrane proteolysis and endoplasmic reticulum retention of hepatitis C virus core protein
Q48083467Intron loss from the NADH dehydrogenase subunit 4 gene of lettuce mitochondrial DNA: evidence for homologous recombination of a cDNA intermediate
Q53438414Introns enable the polyomavirus middle and small T antigens to stimulate the growth of primary rat embryo fibroblasts.
Q31167074Isolating microsatellite DNA loci
Q28833781Isolation and characterization of 24 polymorphic microsatellite loci for the study of genetic population structure of the sheepshead Archosargus probatocephalus (Actinopterygii, Perciformes, Sparidae)
Q38313666Isolation and characterization of an Arabidopsis thaliana gene for the 54 kDa subunit of the signal recognition particle
Q39457849Isolation and characterization of human immunodeficiency virus type 1 variants infectious to brain-derived cells: detection of common point mutations in the V3 region of the env gene of the variants.
Q42649767Isolation and characterization of the carotenoid biosynthesis genes of Flavobacterium sp. strain R1534.
Q32004974Isolation and sequence of cDNA encoding the motilin precursor from monkey intestine. Demonstration of the motilin precursor in the monkey brain
Q36684584Isolation of cDNA encoding a novel serpin of crayfish hemocytes
Q36784628Isolation of cytochrome P-450 cDNA clones from the higher plant Catharanthus roseus by a PCR strategy
Q38352745Isolation of laccase gene-specific sequences from white rot and brown rot fungi by PCR.
Q24336535K-glypican: a novel GPI-anchored heparan sulfate proteoglycan that is highly expressed in developing brain and kidney
Q35833643K-ras mutations and p53 alterations in neoplastic and nonneoplastic lesions associated with longstanding ulcerative colitis
Q28189009Kinetic analysis of the interaction of the copper chaperone Atox1 with the metal binding sites of the Menkes protein
Q31151104Knockin' on pHeaven's door: a fast and reliable high-throughput compatible zero-background cloning procedure.
Q24309104LIM-kinase1 hemizygosity implicated in impaired visuospatial constructive cognition
Q54177537Large-scale cloning of human chromosome 2-specific yeast artificial chromosomes (YACs) using an interspersed repetitive sequences (IRS)-PCR approach
Q42746364Ligation independent cloning vectors for expression of SUMO fusions
Q46709489Lipid signaling in plants. Cloning and expression analysis of the obtusifoliol 14alpha-demethylase from Solanum chacoense Bitt., a pollination- and fertilization-induced gene with both obtusifoliol and lanosterol demethylase activity
Q33590308Localization of symbiotic clostridia in the mixed segment of the termite Nasutitermes takasagoensis (Shiraki).
Q31994612Lumbricin I, a novel proline-rich antimicrobial peptide from the earthworm: purification, cDNA cloning and molecular characterization.
Q33969530MDP1, a Saccharomyces cerevisiae gene involved in mitochondrial/cytoplasmic protein distribution, is identical to the ubiquitin-protein ligase gene RSP5.
Q36762593Mapping and cloning of the critical region for the spinocerebellar ataxia type 1 gene (SCA1) in a yeast artificial chromosome contig spanning 1.2 Mb.
Q41063822Mechanisms of transcription activation exerted by GadX and GadW at the gadA and gadBC gene promoters of the glutamate-based acid resistance system in Escherichia coli
Q48462053Messenger ribonucleic acid for transforming growth factor-alpha, but not for epidermal growth factor, is expressed in fetal and neonatal mouse brain
Q47915822Metazoan cellulase genes from termites: intron/exon structures and sites of expression
Q36710639Molecular and biochemical characterization of a protective 40-kilodalton antigen from Corynebacterium pseudotuberculosis
Q86593147Molecular and biological studies on male-sterile cytoplasm in the Cruciferae. III. Distribution of Ogura-type cytoplasm among Japanese wild radishes and Asian radish cultivars
Q32064844Molecular basis of beta-thalassemia in the Maldives
Q42670518Molecular characterisation of a cDNA sequence encoding the backbone of a style-specific 120 kDa glycoprotein which has features of both extensins and arabinogalactan proteins.
Q28572091Molecular characterization and expression of rat acyl-CoA synthetase 3
Q52546688Molecular characterization of spontaneous mutations at the scarlet locus of Drosophila melanogaster.
Q48067067Molecular characterization of the cinnabar region of Drosophila melanogaster: identification of the cinnabar transcription unit
Q36818758Molecular cloning and characterization of a putative mouse hyaluronan synthase
Q34055753Molecular cloning and characterization of the gene coding for the aerobic azoreductase from Xenophilus azovorans KF46F.
Q24313431Molecular cloning and characterization of the human and mouse UDP-glucose dehydrogenase genes
Q36788862Molecular cloning and expression analysis of peroxidase genes from wheat
Q24563478Molecular cloning and expression of the 32-kDa subunit of human TFIID reveals interactions with VP16 and TFIIB that mediate transcriptional activation
Q24310139Molecular cloning of a novel human diacylglycerol kinase highly selective for arachidonate-containing substrates
Q28115036Molecular cloning of cDNAs of mouse peptidylarginine deiminase type I, type III and type IV, and the expression pattern of type I in mouse
Q35188905Molecular cloning of the gene encoding the mosaic neurotoxin, composed of parts of botulinum neurotoxin types C1 and D, and PCR detection of this gene from Clostridium botulinum type C organisms
Q34032165Molecular considerations for development of phage antibody libraries.
Q41592490Molecular diversity of neuronal-type calcium channels identified in small cell lung carcinoma
Q38555935Molecular evolution of a portion of the mitochondrial 16S ribosomal gene region in scleractinian corals
Q42675094Molecular evolution of cdc2 pseudogenes in spruce (Picea).
Q28776499Molecular genetics of ecological diversification: duplication and rapid evolution of toxin genes of the venomous gastropod Conus
Q35642967Molecular mapping of a recombination hotspot located in the second intron of the human TAP2 locus
Q47708811Molecular markers linked to breeding system differences in segregating and natural populations of the cereal aphid Rhopalosiphum padi L.
Q39788908Molecular phylogenetic exploration of bacterial diversity in a Bakreshwar (India) hot spring and culture of Shewanella-related thermophiles
Q47350059Molecular systematics of aphids (Homoptera: Aphididae): new insights from the long-wavelength opsin gene
Q37632226Molecularly engineered resistance to California serogroup virus replication in mosquito cells and mosquitoes
Q36688469Mosquito carboxylesterase Est alpha 2(1) (A2). Cloning and sequence of the full-length cDNA for a major insecticide resistance gene worldwide in the mosquito Culex quinquefasciatus
Q48202870Mouse chromosome-specific markers generated by PCR and their mapping through interspecific backcrosses
Q36842954Mrs, a new subfamily of Tourist transposable elements
Q44326628Multiple alleles encoding a virus-like particle protein in the ichneumonid endoparasitoid Venturia canescens
Q48052911Multiple transcription start sites of the carrot dihydrofolate reductase-thymidylate synthase gene, and sub-cellular localization of the bifunctional protein
Q34319775Murine muscular dystrophy caused by a mutation in the laminin alpha 2 (Lama2) gene
Q41100130Mutation analysis of chromosome 19 calmodulin (CALM3) gene in Alzheimer's disease patients
Q35171833Mutation in the relA gene of Vibrio cholerae affects in vitro and in vivo expression of virulence factors
Q35828610Mutational analysis of the murine AIDS-defective viral genome reveals a high reversion rate in vivo and a requirement for an intact Pr60gag protein for efficient induction of disease
Q34733805Mutations in the TRKA/NGF receptor gene in patients with congenital insensitivity to pain with anhidrosis
Q40915825Mutations uncouple human fibroblast growth factor (FGF)-7 biological activity and receptor binding and support broad specificity in the secondary receptor binding site of FGFs.
Q48061831Mutualistic fungal endophytes express a proteinase that is homologous to proteases suspected to be important in fungal pathogenicity
Q36634970Myristylation of Pr60gag of the murine AIDS-defective virus is required to induce disease and notably for the expansion of its target cells
Q24682409NF-Y and Sp1/Sp3 are involved in the transcriptional regulation of the peptidylarginine deiminase type III gene (PADI3) in human keratinocytes
Q30304475NRSA-1: a resistance gene homolog expressed in roots of non-host plants following parasitism by Striga asiatica (witchweed).
Q78222429New adipogenic cell lines derived from C3H10T1/2
Q42599635Non-recombinant background in gene targeting: illegitimate recombination between a hpt gene and a defective 5' deleted nptII gene can restore a Kmr phenotype in tobacco
Q84686003Novel GPS-containing G protein-coupled receptor from Monosiga brevicollis
Q48000332Novel sequence organization and insertion specificity of IS605 and IS606: chimaeric transposable elements of Helicobacter pylori
Q71143510Nucleosome assembly on CTG triplet repeats
Q40536269Nucleotide sequence of the cDNA encoding silk gland elongation factor 1 beta'.
Q48086760Nucleotide sequence of the equine interferon gamma cDNA.
Q43208500One-step preparation of a TA-cloning vector from a specially designed parent plasmid containing a dual lacZ gene system
Q42596374Organ-specific reduction in the abundance of a mitochondrial protein accompanies fertility restoration in cytoplasmic male-sterile radish.
Q56883147Origins of diverse feeding ecologies within Conus, a genus of venomous marine gastropods
Q35062969Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids
Q73025384Overlapping expression and redundant activation of mesenchymal fibroblast growth factor (FGF) receptors by alternatively spliced FGF-8 ligands
Q74285400Overlapping expression of cytosolic glutamine synthetase and phenylalanine ammonia-lyase in immature leaf blades of rice
Q39560513Overproduction of L-cysteine and L-cystine by Escherichia coli strains with a genetically altered serine acetyltransferase
Q35011326PCR using a thermostable polymerase with 3' to 5' exonuclease activity generates blunt products suitable for direct cloning
Q73835872PCR-based plasmid vector construction for generation of recombinant viruses
Q24670311Pacifastin, a novel 155-kDa heterodimeric proteinase inhibitor containing a unique transferrin chain
Q39582553Particle polymorphism caused by deletion of a peptide molecular switch in a quasiequivalent icosahedral virus
Q27480371Pathogen-derived resistance to dengue type 2 virus in mosquito cells by expression of the premembrane coding region of the viral genome
Q36734042Peach (Prunus persica) endopolygalacturonase cDNA isolation and mRNA analysis in melting and nonmelting peach cultivars
Q40610337Pheromone binding proteins of Epiphyas postvittana (Lepidoptera: Tortricidae) are encoded at a single locus
Q28571582Phosphorylation events associated with different states of activation of a hepatic cardiolipin/protease-activated protein kinase. Structural identity to the protein kinase N-type protein kinases
Q42632016Phylogenetic affinities of the grasses to other monocots as revealed by molecular analysis of chloroplast DNA.
Q111622934Phylogenetic analysis of Trichaptum based on nuclear 18S, 5.8S and ITS ribosomal DNA sequences
Q33708198Phylogenetic evidence for the existence of novel thermophilic bacteria in hot spring sulfur-turf microbial mats in Japan.
Q32064966Phylogenetic placement of rickettsiae from the ticks Amblyomma americanum and Ixodes scapularis
Q36860297Pituitary homeobox 2, a novel member of the bicoid-related family of homeobox genes, is a potential regulator of anterior structure formation.
Q79774630Plant-endophyte interplay protects tomato against a virulent Verticillium
Q33984050Plasmid-encoded anthranilate synthase (TrpEG) in Buchnera aphidicola from aphids of the family pemphigidae
Q24562119Platelet-activating factor acetylhydrolase deficiency. A missense mutation near the active site of an anti-inflammatory phospholipase
Q68147477Point mutations and polymorphisms in the human dystrophin gene identified in genomic DNA sequences amplified by multiplex PCR
Q36104031Polyketide proofreading by an acyltransferase-like enzyme
Q36468695Polymerase recognition of synthetic oligodeoxyribonucleotides incorporating degenerate pyrimidine and purine bases
Q52582439Polyphenol oxidase from hybrid poplar. Cloning and expression in response to wounding and herbivory.
Q24311437Positional cloning of a novel potassium channel gene: KVLQT1 mutations cause cardiac arrhythmias
Q54611152Positive-selection and ligation-independent cloning vectors for large scale in planta expression for plant functional genomics.
Q36115661Predictive value of vascular endothelial growth factor (VEGF) in metastasis and prognosis of human colorectal cancer
Q30653700Preparation of a genomic library using a TA vector
Q74095285Presence of a conserved domain of GATA transcription factors in Echinococcus granulosus
Q33899584Prognostic implication of human papillomavirus infection in squamous cell carcinoma of the lung
Q30658856Properties of the prophenoloxidase activating enzyme of the freshwater crayfish, Pacifastacus leniusculus
Q28263365Prosystemin from potato, black nightshade, and bell pepper: primary structure and biological activity of predicted systemin polypeptides
Q38360412Pumilio homologue from saprolegnia parasitica specifically expressed in undifferentiated spore cysts
Q39846634Putative evolutionary origin of plasmids carrying the genes involved in leucine biosynthesis in Buchnera aphidicola (endosymbiont of aphids).
Q60949729Pyridine Nucleotide Coenzyme Specificity of -Hydroxybenzoate Hydroxylase and Related Flavoprotein Monooxygenases
Q42620928RNA editing in ATPase subunit 6 mRNAs in Oenothera mitochondria. A new termination codon shortens the reading frame by 35 amino acids
Q40400052RNA editing of transcripts of a chimeric mitochondrial gene associated with cytoplasmic male-sterility in Brassica
Q40038492RNA splicing in Borna disease virus, a nonsegmented, negative-strand RNA virus.
Q36693569RNA trans-splicing in flatworms. Analysis of trans-spliced mRNAs and genes in the human parasite, Schistosoma mansoni
Q42689515Rapid and efficient construction of yeast artificial chromosome contigs in the mouse genome with interspersed repetitive sequence PCR (IRS-PCR): generation of a 5-cM, > 5 megabase contig on mouse chromosome 1.
Q30667997Rapid isolation of genes from an indexed genomic library of C. cinereus in a novel pab1+ cosmid
Q44174226Rapid, nonradioactive differential display using Tth polymerase
Q39720084Rational primer design greatly improves differential display-PCR (DD-PCR).
Q33602957Reactivity profile of human anti-82-kilodalton rhoptry protein antibodies generated during natural infection with Plasmodium falciparum
Q35397147Recent striking changes in histological differentiation and rate of human papillomavirus infection in squamous cell carcinoma of the lung in Okinawa, a subtropical island in southern Japan
Q35833427Recombination between feline exogenous and endogenous retroviral sequences generates tropism for cerebral endothelial cells.
Q42422797Recombination-assisted megaprimer (RAM) cloning
Q28582864Reduced expression of kan-1 (encoding putative bile acid-CoA-amino acid N-acyltransferase) mRNA in livers of rats after partial hepatectomy and during sepsis
Q35841523Region E3 of subgroup B human adenoviruses encodes a 16-kilodalton membrane protein that may be a distant analog of the E3-6.7K protein of subgroup C adenoviruses.
Q48063869Regional assignment of human ESTs by whole-genome radiation hybrid mapping
Q35030830Regulation of the alpha-galactosidase activity in Streptococcus pneumoniae: characterization of the raffinose utilization system
Q38318702Regulation of the proneural gene achaete by helix-loop-helix proteins
Q40651668Repression of HPV E6-activated RSV promoter activity by anti-cancer agents
Q34763006Rice-barley synteny and its application to saturation mapping of the barley Rpg1 region.
Q36728275Robust one-tube Ω-PCR strategy accelerates precise sequence modification of plasmids for functional genomics
Q64897511Role of endothelin as a mitogen in experimental glomerulonephritis in rats.
Q36740605S-Adenosylhomocysteine hydrolase deficiency: a second patient, the younger brother of the index patient, and outcomes during therapy
Q34546376S-adenosylhomocysteine hydrolase deficiency in a human: a genetic disorder of methionine metabolism
Q24317024SCN5A mutations associated with an inherited cardiac arrhythmia, long QT syndrome
Q31155269SDA, a DNA aptamer inhibiting E- and P-selectin mediated adhesion of cancer and leukemia cells, the first and pivotal step in transendothelial migration during metastasis formation
Q48055293Screening of fungi for the presence of the trichodiene synthase encoding sequence by hybridization to the Tri5 gene cloned from Fusarium poae
Q42685171Seedling lethality in Nicotiana plumbaginifolia conferred by Ds transposable element insertion into a plant-specific gene
Q34300755Selection of primers for polymerase chain reaction
Q36039938Sequence analysis of teleost retina-specific lactate dehydrogenase C: evolutionary implications for the vertebrate lactate dehydrogenase gene family
Q48038418Sequence analysis of the actA gene of Listeria monocytogenes isolated from human
Q30865880Sequence conservation and expression of the sex-lethal homologue in the fly Megaselia scalaris
Q36618782Sequence conservation in field and experimental isolates of Borna disease virus.
Q46468573Sequence divergence of B2m alleles of wild Mus musculus and Mus spretus implies positive selection
Q40806989Sequence variability within the tobacco retrotransposon Tnt1 population.
Q73177349Sequence variation of allele 27 at the D1S80 locus
Q48056777Sequence, overproduction and purification of Vibrio proteolyticus ribosomal protein L18 for in vitro and in vivo studies
Q42083492Sequencing and heterologous expression of an epimerase and two lyases from iminodisuccinate-degrading bacteria
Q34267666Shadoo, a new protein highly conserved from fish to mammals and with similarity to prion protein
Q39588592Simultaneous RT/PCR detection and differentiation of arabis mosaic and grapevine fanleaf nepoviruses in grapevines with a single pair of primers.
Q38332056Single Primer-Mediated Polymerase Chain Reaction: Application in Cloning of Two Different 5′-Untranslated Sequences of Acidic Fibroblast Growth Factor mRNA
Q111622834Small subunit ribosomal RNA gene phylogeny of Plasmodiophora brassicae
Q39771270Solid-phase cloning for high-throughput assembly of single and multiple DNA parts
Q39581936Specific encapsidation of nodavirus RNAs is mediated through the C terminus of capsid precursor protein alpha.
Q35606522Specific in vivo protein-protein interactions between Escherichia coli SOS mutagenesis proteins
Q33919147Squamous metaplasia induced by transfection of human papillomavirus DNA into cultured adenocarcinoma cells
Q45877531Stable recombinant expression and characterization of the two haemophilic factor VIII variants C329S (CRM(-)) and G1948D (CRM(r)).
Q44322862Stimulation of peripheral blood mononuclear cells with lipopolysaccharide induces expression of the plasma protein alpha2-macroglobulin
Q48046059Stromal and thylakoid-bound ascorbate peroxidases are produced by alternative splicing in pumpkin.
Q64921983Structural features of GmIRCHS, candidate of the I gene inhibiting seed coat pigmentation in soybean: implications for inducing endogenous RNA silencing of chalcone synthase genes.
Q28259400Structural organization of the human cardiac alpha-myosin heavy chain gene (MYH6)
Q42672196Structure and evolution of the mitochondrial genome of Exorista sorbillans: the Tachinidae (Diptera: Calyptratae) perspective
Q30854326Structure and promoter analysis of the human unc-33-like phosphoprotein gene. E-box required for maximal expression in neuroblastoma and myoblasts
Q33852376Structure of the gene mutated in glycogen storage disease type Ib.
Q48085563Structure of the large ribosomal subunit RNA of Phytophthora megasperma, and phylogeny of the oomycetes
Q33963214Structure, expression and phylogenetic analysis of the gene encoding actin I in Pneumocystis carinii.
Q48064450Sugar accumulation in grape berries. Cloning of two putative vacuolar invertase cDNAs and their expression in grapevine tissues
Q39874498Targeting human immunodeficiency virus type 1 reverse transcriptase by intracellular expression of single-chain variable fragments to inhibit early stages of the viral life cycle.
Q39567182Tetrachloroethene dehalogenase from Dehalospirillum multivorans: cloning, sequencing of the encoding genes, and expression of the pceA gene in Escherichia coli.
Q22008635The 100-kDa 2',5'-oligoadenylate synthetase catalyzing preferentially the synthesis of dimeric pppA2'p5'A molecules is composed of three homologous domains
Q35167776The Arabidopsis thaliana apurinic endonuclease Arp reduces human transcription factors Fos and Jun
Q39801512The Bacillus thuringiensis vegetative insecticidal protein Vip3A lyses midgut epithelium cells of susceptible insects
Q48026833The Bactrocera tryoni homologue of the Drosophila melanogaster sex-determination gene doublesex
Q41947627The CRY1 gene in Chlamydomonas reinhardtii: structure and use as a dominant selectable marker for nuclear transformation
Q28296947The Desmocollins of Human Foreskin Epidermis: Identification and Chromosomal Assignment of a Third Gene and Expression Patterns of the Three Isoforms
Q41344671The Down regulated in Adenoma (dra) gene encodes an intestine-specific membrane sulfate transport protein
Q38290268The Identification of the Single‐Stranded DNA‐Binding Domain of the Escherichia coli RecA Protein
Q33198534The PRESAT-vector: asymmetric T-vector for high-throughput screening of soluble protein domains for structural proteomics
Q48079446The S-ribonuclease gene of Petunia hybrida is expressed in nonstylar tissue, including immature anthers
Q36566167The Sex-lethal early splicing pattern uses a default mechanism dependent on the alternative 5' splice sites
Q28255655The V kappa genes of the L regions and the repertoire of V kappa gene sequences in the human germ line
Q24564339The classical human phosphoglucomutase (PGM1) isozyme polymorphism is generated by intragenic recombination
Q41870316The conservative substitution Asp-645-->Glu in lysosomal alpha-glucosidase affects transport and phosphorylation of the enzyme in an adult patient with glycogen-storage disease type II.
Q30650558The crayfish plasma clotting protein: a vitellogenin-related protein responsible for clot formation in crustacean blood
Q35194851The gene for autosomal dominant spinocerebellar ataxia (SCA1) maps centromeric to D6S89 and shows no recombination, in nine large kindreds, with a dinucleotide repeat at the AM10 locus
Q34355151The gene involved in X-linked agammaglobulinaemia is a member of the src family of protein-tyrosine kinases
Q35851363The growth defect in Escherichia coli deficient in peptidyl-tRNA hydrolase is due to starvation for Lys-tRNA(Lys)
Q42160734The independent gene amplification of electrophoretically indistinguishable B esterases from the insecticide-resistant mosquito Culex quinquefasciatus
Q28145304The intron-containing L3 ribosomal protein gene (RPL3): sequence analysis and identification of U43 and of two novel intronic small nucleolar RNAs
Q44644430The laccase gene family in Coprinopsis cinerea (Coprinus cinereus).
Q24316923The molecular basis of Sanfilippo syndrome type B
Q42612922The murine cot proto-oncogene: genome structure and tissue-specific expression.
Q34433090The murine voltage-dependent anion channel gene family. Conserved structure and function
Q30661423The novel sequences of major plasma apolipoproteins in the eel Anguilla japonica
Q48283217The period gene in two species of tephritid fruit fly differentiated by mating behaviour
Q89017665The plasmid vectors, pBS2ndd and pBS3ndd, for versatile cloning with low background in Escherichia coli
Q46273713The role of short homology repeats and TdT in generation of the invariant gamma delta antigen receptor repertoire in the fetal thymus.
Q40497047The terminal 5' phosphate and proximate phosphorothioate promote ligation-independent cloning
Q33889345The yeast Ty3 retrotransposon contains a 5'-3' bipartite primer-binding site and encodes nucleocapsid protein NCp9 functionally homologous to HIV-1 NCp7
Q67577208Three novel mutations of the ornithine aminotransferase (OAT) gene in gyrate atrophy
Q33374213Tomato phenylalanine ammonia-lyase gene family, highly redundant but strongly underutilized
Q46897862Transcriptional co-regulation of evolutionarily conserved microRNA/cone opsin gene pairs: implications for photoreceptor subtype specification
Q34305433Transposable element ISHp608 of Helicobacter pylori: nonrandom geographic distribution, functional organization, and insertion specificity
Q51415863Turnover and down-regulation of GABA(A) receptor alpha1, beta2S, and gamma1 subunit mRNAs by neurons in culture.
Q41582322Two mutations affecting the transport and maturation of lysosomal alpha-glucosidase in an adult case of glycogen storage disease type II.
Q39468019Typing of Candida glabrata in clinical isolates by comparative sequence analysis of the cytochrome c oxidase subunit 2 gene distinguishes two clusters of strains associated with geographical sequence polymorphisms
Q48083113Unique intronic variations of HLA-DQ beta gene in early-onset periodontitis
Q28250990Unstable expansion of CAG repeat in hereditary dentatorubral-pallidoluysian atrophy (DRPLA)
Q38348217Use of an arbitrarily primed PCR product in the development of a Campylobacter jejuni-specific PCR.
Q30658725Use of serial analysis of gene expression (SAGE) technology
Q48073370cDNA Cloning, Overexpression in Escherichia coli, Purification and Characterization of Sheep Liver Cytosolic Serine Hydroxymethyltransferase
Q36819604cDNA cloning and expression of a gene for 3-ketoacyl-CoA thiolase in pumpkin cotyledons
Q58856615cDNA cloning of turtle prion protein
Q41140791cDNA cloning, baculovirus-expression and kinetic properties of the esterase, E3, involved in organophosphorus resistance in Lucilia cuprina
Q33367472cDNA sequence and expression of an intron-containing histone H2A gene from Norway spruce, Picea abies
Q24671810eat-5 and unc-7 represent a multigene family in Caenorhabditis elegans involved in cell-cell coupling
Q24310553hDIP--a potential transcriptional regulator related to murine TSC-22 and Drosophila shortsighted (shs)--is expressed in a large number of human tissues
Q40788661hsk1+, a Schizosaccharomyces pombe gene related to Saccharomyces cerevisiae CDC7, is required for chromosomal replication
Q35618784nfi, the gene for endonuclease V in Escherichia coli K-12.
Q34786037p27KIP1 deletions in childhood acute lymphoblastic leukemia
Q40696522p53-dependent downregulation of metastasis-associated laminin receptor
Q38967564pUCPCR1. A vector for direct cloning of PCR products in a double Xcm1 restriction site offering compatible single 3'-overhanging T residues
Q50441939pYEMF, a pUC18-Derived XcmI T-Vector for Efficient Cloning of PCR Products

Search more.