Analysis of enzyme reactions in situ

scientific article published on February 1995

Analysis of enzyme reactions in situ is …
instance of (P31):
review articleQ7318358
scholarly articleQ13442814

External links are
P356DOI10.1007/BF00243905
P698PubMed publication ID7775194

P2093author name stringC J Van Noorden
G N Jonges
P2860cites workMutations in the glucose-6-phosphatase gene that cause glycogen storage disease type 1aQ24317268
Formation and detection of RNA-DNA hybrid molecules in cytological preparationsQ28247194
The interaction of rat liver carbamoyl phosphate synthetase and ornithine transcarbamoylase with inner mitochondrial membranesQ28579123
The application of continuous monitoring microdensitometry to an analysis of NAD+ binding and 3 beta-hydroxy-delta 5-steroid dehydrogenase activity in the regressing corpus luteum of the pro-oestrous rat ovaryQ28581540
Metabolic dependence of glycolytic enzyme binding in rat and sheep heartQ30040516
The effect of volume occupancy upon the thermodynamic activity of proteins: some biochemical consequencesQ30413607
A multipurpose instrument for quantitative intravital microscopyQ33200926
Quantitation in histochemistry: A review of some commercially available microdensitometersQ34023592
Metabolite transfer via enzyme-enzyme complexesQ34050699
Properties and metabolism of the aqueous cytoplasm and its boundariesQ34055497
Substrate channeling of oxalacetate in solid-state complexes of malate dehydrogenase and citrate synthaseQ34200906
How crowded is the cytoplasm?Q34268384
Brain cytochrome oxidase: purification, antibody production, and immunohistochemical/histochemical correlations in the CNS.Q34456311
Aminopeptidase A is angiotensinase A. II. Biochemical studies on aminopeptidase A and M in rat kidney homogenateQ34707316
Aldolase exists in both the fluid and solid phases of cytoplasmQ35105272
Hepatocyte Heterogeneity in the Metabolism of CarbohydratesQ35188729
Liver ArchitectureQ35188754
Quantitative immunohistochemistry. Theoretical background and its application in biology and surgical pathologyQ35230983
The evolution of proteinase substrates with special reference to dipeptidylpeptidase IV.Q35270063
Metabolic zonation of the liver: regulation and implications for liver functionQ35417325
Cell volume and hormone actionQ35431815
Regulation of proteinase activation in mammalian tissues.Q35777073
A quantitative stereological description of the ultrastructure of normal rat liver parenchymal cellsQ36189494
Regulation of glucose-6-phosphate dehydrogenase activity in sea urchin eggs by reversible association with cell structural elementsQ36215629
Periportal and pericentral pyridine nucleotide fluorescence from the surface of the perfused liver: evaluation of the hypothesis that chronic treatment with ethanol produces pericentral hypoxiaQ36311208
Permeabilization of animal cells for kinetic studies of intracellular enzymes: in situ behavior of the glycolytic enzymes of erythrocytesQ36413390
Cell volume in the regulation of hepatic function: a mechanism for metabolic controlQ36447730
2-Deoxyglucose and cytochalasin D modulate aldolase mobility in living 3T3 cellsQ36531739
Errors in microdensitometryQ36589843
Flow-cytometric monitoring of intracellular flavins simultaneously with NAD(P)H levelsQ36617131
Geographical mapping of metabolites in biological tissue with quantitative bioluminescence and single photon imagingQ36696660
Regulation of enzyme activity in the cell: effect of enzyme concentrationQ36831031
Enzyme Histochemistry as a Link between Biochemistry and MorphologyQ37236621
Enzyme stimulation upon fertilization is revealed in electrically permeabilized sea urchin eggsQ37485831
The contribution of microchemical methods of histochemistry to the biological sciencesQ37619304
Nitrogen metabolism and ornithine cycle functionQ37937542
Enzyme-enzyme interactions and their metabolic roleQ37946884
Principles of cytophotometry in enzyme histochemistry and validity of the reactionsQ38232896
Functional specialization of different hepatocyte populationsQ38663205
Polyvinyl alcohol and other tissue protectants in enzyme histochemistry: a consumer's guideQ38697292
Methods for the study of liver cell heterogeneityQ38731236
A quantitative histochemical technique for the characterisation of ?-glucosidases in the brush-border membrane of rat jejunumQ39287911
Interaction of calf brain tubulin with poly(ethylene glycols)Q39291821
Prospects for microscopical cytochemistryQ39408134
Expression of alkaline phosphatase in the mature mouse placenta visualized by in situ hybridization and enzyme histochemistryQ39408369
Old pathway--new concept: control of glycolysis by metabolite-modulated dynamic enzyme associationsQ39577805
Biophysical chemistry of metabolic reaction sequences in concentrated enzyme solution and in the cellQ39657733
Immunofluorescent localization of glycogenolytic and glycolytic enzyme proteins and of malate dehydrogenase isozymes in cross-striated skeletal muscle and heart of the rabbitQ39736251
Cell and tissue fixation, 1972-1982.Q39849008
Permeabilized cellsQ40103595
Interactions between soluble enzymes and subcellular structureQ40319811
Multichannel microspectrofluorometry for topographic and spectral analysis of NAD(P)H fluorescence in single living cellsQ40330914
Association of Glyceraldehyde-3-Phosphate Dehydrogenase with the Particulate Fraction of Chicken Skeletal MuscleQ40342451
A user's guide for avoiding errors in absorbance image cytometry: a review with original experimental observationsQ40388415
The Constant Proportion Enzyme Group Concept in the Selection of Reference Enzymes in MetabolismQ40458323
Practical aspects of radio-isotopic in situ hybridization on RNAQ40486844
A re-evaluation of the tissue distribution and physiology of xanthine oxidoreductaseQ40615748
In situ kinetic parameters of glucose-6-phosphate dehydrogenase and phosphogluconate dehydrogenase in different areas of the rat liver acinusQ41282495
Heterogeneous distribution of glutamine synthetase among rat liver parenchymal cells in situ and in primary culture.Q41449098
Kinetic studies on the removal of extracellular hydrogen peroxide by cultured fibroblastsQ41494700
A new assay for intracellular measurement of inosine monophosphate dehydrogenase activity: a guide for better selection of patients for enzyme-targeted chemotherapyQ41503329
Measuring rates of O2 uptake in periportal and pericentral regions of liver lobule: stop-flow experiments with perfused liverQ41606539
Microcompartmentation of glycolytic enzymes in cultured cellsQ41611923
The application of a novel biotinylated affinity label for the detection of a cathepsin B-like precursor produced by breast-tumour cells in cultureQ41628537
Precipitation of complexes between glutamate dehydrogenase and mitochondrial enzymesQ41629545
Rapid release of bound glucose-6-phosphate dehydrogenase by growth factors. Correlation with increased enzymatic activityQ41675294
Comparison of the effects of various amino acids on glycogen synthesis, lipogenesis and ketogenesis in isolated rat hepatocytesQ41774211
Hepatic enzymic zonation: a reevaluation of the concept of the liver acinusQ41828928
Modification of the kinetic parameters of aldolase on binding to the actin-containing filaments of skeletal muscleQ41854717
Microwave dielectric properties of tissue. Some comments on the rotational mobility of tissue waterQ42420667
Early changes in brush border disaccharidase kinetics in rat jejunum following subcutaneous administration of tetraiodothyronine: a quantitative histochemical study on villi revealing normal morphologyQ42463642
Kinetic characterization of unspecific alkaline phosphatase at different villus sites of rat jejunum. A quantitative histochemical studyQ42473979
Procathepsin L-specific antibodies that recognize procathepsin L but not cathepsin L.Q42809751
Cysteine endopeptidases and their inhibitors in malignant progression of rat embryo fibroblastsQ42818351
New class of sensitive and selective fluorogenic substrates for serine proteinases. Amino acid and dipeptide derivatives of rhodamineQ42865532
Rhodamine-based compounds as fluorogenic substrates for serine proteinasesQ42869276
Flow cytofluorometric analysis of enzyme reactions based on quenching of fluorescence by the final reaction product: detection of glucose-6-phosphate dehydrogenase deficiency in human erythrocytesQ43790043
RNA-DNA Hybrids at the Cytological LevelQ43870738
A microspectrofluorometric study of the effect of anthralin, an antipsoriatic drug, on cellular structures and metabolismQ43990143
Regulation of xanthine oxidase activity and immunologically detectable protein in rats in response to dietary protein and ironQ44114113
A Xanthinuric Family - The Proposita Having Immunologically Reactive Xanthine Oxidase but no Xanthine Oxidase ActivityQ44434446
Molecular basis for the transfer of nicotinamide adenine dinucleotide among dehydrogenasesQ45254616
Quantitative histochemical determination of succinic dehydrogenase activity in skeletal muscle fibres.Q45959606
Marked and variable inhibition by chemical fixation of cytochrome oxidase and succinate dehydrogenase in single motoneuronsQ46082737
Channeling of urea cycle intermediates in situ in permeabilized hepatocytesQ46162021
The use of caged substrates to assess the activity of 6-phosphogluconate dehydrogenase in living sea urchin eggsQ46821797
Microtubular protein in its polymerized or nonpolymerized states differentially modulates in vitro and intracellular fluxes catalyzed by enzymes of carbon metabolismQ48128830
The dependence of phosphofructokinase kinetics upon protein concentrationQ48579664
Comparative immunohistochemistry and histochemistry of dipeptidyl peptidase IV in rat organs during developmentQ48892697
Regulation of cytochrome oxidase protein levels by functional activity in the macaque monkey visual system.Q49041921
The diverse Michaelis constants and maximum velocities of lactate dehydrogenase in situ in various types of cellQ49109698
Flow cytometric analysis of protease activities in vital cells.Q50792267
NADH-dependent dehydrogenase activity estimation by flow cytometric analysis of 3-(4,5-dimethylthiazolyl-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction.Q50796612
Methods of microphotometric assay of succinate dehydrogenase and cytochrome c oxidase activities for use on human skeletal muscle.Q50886285
Quantitative cytochemistry of beta-galactosidase in normal and enzyme deficient (gal) pollen of Brassica campestris: application of the indigogenic method.Q50919752
20 alpha-Hydroxysteroid dehydrogenase activity in the rat corpus luteum; a quantitative cytochemical study.Q50933237
Study on dipeptidylpeptidase II (DPP II).Q50945435
The Glucose/Glucose-6-Phosphate Cycle in the Periportal and Perivenous Zone of Rat LiverQ51651500
Estimating the initial reaction velocity of a soluble dehydrogenase in situ.Q52399840
Rapid kinetics of liver microsomal glucose-6-phosphatase. Evidence for tight-coupling between glucose-6-phosphate transport and phosphohydrolase activity.Q52453481
Intracellular localization of glycolytic enzymes in cros-striated muscles of Locusta migratoria.Q52628030
Metabolic control and compartmentation in single living cells.Q52707005
Effect of macromolecular crowding upon the structure and function of an enzyme: glyceraldehyde-3-phosphate dehydrogenaseQ52735101
Letter: The Feulgen reaction in polyvinyl alcohol or polyethylene glycol solution. "Fixation" by excluded volume.Q53828666
Quantitative determination of calcium-activated myosin adenosine triphosphatase activity in rat skeletal muscle fibresQ54313553
Association of glyceraldehyde-3-phosphate dehydrogenase with the plasma membrane of the intact human red blood cell.Q54355469
Rapid microspectrofluorimetry for biochemical and metabolic studies in single living cells.Q54664506
In situ assay of intracellular enzymes of yeast (Kluyveromyces fragilis) by digitonin permeabilization of cell membraneQ67246423
A quantitative study of the effects of different grades of polyvinyl alcohol on the activities of certain enzymes in unfixed tissue sectionsQ67355474
Molecular extinction coefficients of lead sulfide and polymerized diaminobenzidine as final reaction products of histochemical phosphatase reactionsQ67507359
The use of unfixed cryostat sections for electron microscopic study of D-amino acid oxidase activity in rat liverQ67524244
Novel method for performing carbonic anhydrase histochemistry and immunocytochemistry on cryosectionsQ67538703
Initial reaction kinetics of succinate dehydrogenase in mouse liver studied with a real-time image analyser systemQ67577975
Immunohistochemical demonstration of glutamate dehydrogenase in the postnatally developing rat hippocampal formation and cerebellar cortex: Comparison to activity stainingQ67687428
Anomalous properties of water in macromolecular gelsQ67811062
Mechanisms of dramatic fluctuations of ornithine decarboxylase activity upon tonicity changes in primary cultured rat hepatocytesQ67812875
Activation of NADPH oxidase in human neutrophils permeabilized with Staphylococcus aureus alpha-toxin. A lower Km when the enzyme is activated in situQ67841159
In situ kinetic parameters of glucose-6-phosphatase in the rat liver lobulusQ67841850
Mechanism of activation of liver glycogen synthase by swellingQ67880919
Membrane permeable fluorogenic rhodamine substrates for selective determination of cathepsin LQ68031234
Intraacinar profiles of alcohol dehydrogenase and aldehyde dehydrogenase activities in human liverQ68063459
Imaging neutrophil activation: analysis of the translocation and utilization of NAD(P)H-associated autofluorescence during antibody-dependent target oxidationQ68184864
Nutritional and gonadal effects on the intra-acinar profiles of low-Km and high-Km aldehyde dehydrogenase activity in rat liverQ68347815
Intracellular localization of glycogenolytic and glycolytic enzymes in white and red rabbit skeletal muscle: a gel film method for coupled enzyme reactions in histochemistryQ68439846
Quantitative histochemical analysis of glucose-6-phosphatase activity in rat liver using an optimized cerium-diaminobenzidine methodQ68477989
Quantification of renal Na-K-ATPase activity by image analysing systemQ68492704
Regulation of Escherichia coli phosphofructokinase in situQ68505141
Studies on succinate oxidation. I. The use of intact tissue sectionsQ68608349
Precise cytochemical measurement of neotetrazolium formazan by scanning and integrating microdensitometryQ68649044
Cathepsin B to cysteine proteinase inhibitor balance in metastatic cell subpopulations isolated from murine tumorsQ68920837
The indirect binding of triose-phosphate isomerase to myofibrils to form a glycolytic enzyme mini-complexQ69586726
Heterogeneity of catalase staining in human hepatocellular peroxisomesQ69949034
The chemical determination of section thicknessQ69960299
Perturbation of the quaternary structure and allosteric behavior of rat liver phosphofructokinase by polyethylene glycolQ70039344
Effect of polyethylene glycol on the kinetic behaviour of pyruvate kinase and other potentially regulatory liver enzymesQ70050103
Phosphofructokinase and fructosebisphosphatase from muscle can interact at physiological concentrations with mutual effects on their kinetic behaviorQ70136805
A quantitative histochemical study of dipeptidylpeptidase IV (DPP IV)Q70214129
Channeling of extramitochondrial ornithine to matrix ornithine transcarbamylaseQ70316185
Direct transfer of reduced nicotinamide adenine dinucleotide from glyceraldehyde-3-phosphate dehydrogenase to liver alcohol dehydrogenaseQ70393827
Histochemical localization and quantification of glucose-6-phosphate dehydrogenase in bovine leydig cellsQ70451707
Activation of rabbit muscle phosphofructokinase by F-actin and reconstituted thin filamentsQ70529212
Aminopeptidase A is angiotensinase A- I. Quantitative histochemical studies in the kidney glomerulusQ70602398
Gluconeogenesis predominates in periportal regions of the liver lobuleQ70644524
Identification of an actin binding region in aldolaseQ70647413
Preferential solvent interactions between proteins and polyethylene glycolsQ70656709
Kinetic and morphometric measurements of enzyme reactions in tissue sections with a new instrumental setupQ70874342
Flow cytometric analysis of cellular endogenous fluorescence simultaneously with emission from exogenous fluorochromes, light scatter and absorptionQ70905452
Stimulation of mixed-function oxidation of 7-ethoxycoumarin in periportal and pericentral regions of the perfused rat liver by xylitolQ70999008
Characterization of Isolated Rat‐Liver Cells Made Permeable with FilipinQ71001972
Quantitative and qualitative histochemical investigation on NADP+-dependent dehydrogenases in the limiting plate and the residual parenchyma surrounding terminal hepatic venulesQ71275365
Self-association of the pyruvate dehydrogenase complex from Azotobacter vinelandii in the presence of polyethylene glycolQ71397638
Kinetic aspects of regulation of metabolic processes. The hysteretic enzyme conceptQ71575102
Comparative histochemical, biochemical and immunocytochemical studies of cathepsin B in human gingivaQ71627140
Binding of Aldolase and Triosephosphate Dehydrogenase to F-Actin and Modification of Catalytic Properties of AldolaseQ71652759
Complexes between mitochondrial enzymes and either citrate synthase or glutamate dehydrogenaseQ71658939
Measurement of binding of adenine nucleotides and phosphate to cytosolic proteins in permeabilized rat-liver cellsQ71684178
Localization of active and inactive elastase, alpha-1-proteinase inhibitor, and alpha-2-macroglobulin in human gingivaQ71689073
Combined quantitative immuno- and enzyme cytochemistry of cytochrome c oxidase in sections of neural tissue and cultured cellsQ72058652
A unitary cause for the exclusion of Na+ and other solutes from living cells, suggested by effluxes of Na+, D-arabinose, and sucrose from normal, dying, and dead musclesQ72087420
Cellular hydration state: an important determinant of protein catabolism in health and diseaseQ72218746
Binding of Glycolytic Enzymes to Structure Proteins of the MuscleQ72228501
Ultrathin-layer zone electrophoresis of lactate dehydrogenase isoenzymes in microdissected liver samplesQ72246115
Enzyme concentrations in tissuesQ72297777
Effects of partial hepatectomy, phenobarbital and 3-methylcholanthrene on kinetic parameters of glucose-6-phosphate and phosphogluconate dehydrogenase in situ in periportal, intermediate and pericentral zones of rat liver lobulesQ72422719
Experimentally induced colon cancer metastases in rat liver increase the proliferation rate and capacity for purine catabolism in liver cellsQ72569901
Synthesis and in situ localization of lysosomal alpha-glucosidase in muscle of an unusual variant of glycogen storage disease type IIQ72645446
Quantitative aspects of enzyme histochemistry on sections of freeze-substituted glycol methacrylate-embedded rat liverQ72694748
Ultrathin-layer microelectrophoretic determination of lactate dehydrogenase isoenzymes in corneal and conjunctival epithelium of the cowQ72722431
Localization of uric acid oxidase activity in core and matrix of peroxisomes as detected in unfixed cryostat sections of rat liverQ72725545
Kinetic behaviour of succinate dehydrogenase of three fibre types in skeletal muscle. I. Effects of temperature and a competitive inhibitorQ72753145
A possible mechanism for the Ca-ATPase of sarcoplasmic reticulumQ72788328
Cell swelling and the sensitivity of autophagic proteolysis to inhibition by amino acids in isolated rat hepatocytesQ72863801
Purification of xanthine oxidase from human heartQ72898690
"In situ" determinations of apparent Km and Vmax of brush border disaccharidases along the villi of normal human jejunal biopsy specimens. A quantitative histochemical studyQ72902704
A coordinated set of instruments for optical quantitative high resolution cytochemistryQ74739640
The distributional error in microspectrophotometryQ76180770
Cell heterogeneity within the hepatic lobule of the rat: staining reactionsQ78701050
Reaction rate studies of glucose-6-phosphate dehydrogenase activity in sections of rat liver using four tetrazolium saltsQ93648639
P433issue2
P304page(s)101-118
P577publication date1995-02-01
P1433published inJournal of Molecular HistologyQ2162212
P1476titleAnalysis of enzyme reactions in situ
P478volume27

Reverse relations

cites work (P2860)
Q50757550A novel quantitative histochemical assay to measure endogenous substrate concentrations in tissue sections. Fundamental aspects.
Q53439193Adaptational changes in kinetic parameters of G6PDH but not of PGDH during contamination-induced carcinogenesis in livers of North Sea flatfish.
Q40710017CD26/DPPIV signal transduction function, but not proteolytic activity, is directly related to its expression level on human Th1 and Th2 cell lines as detected with living cell cytochemistry
Q43950379Caged substrates for measuring enzymatic activity in vivo: photoactivated caged glucose 6-phosphate
Q74244571Changes of enzyme activities in the myocardium and skeletal muscle fibres of cardiomyopathic hamsters. A cytophotometrical study
Q41866277Comparison of different detection methods in quantitative microdensitometry
Q78453297Direct comparison of enzyme histochemical and immunohistochemical methods to localize an enzyme
Q34788182Distribution patterns of ornithine decarboxylase in cells and tissues: facts, problems, and postulates.
Q27478394Dynamics of Hepatitis C Virus Replication in Human Liver
Q73222994Effects of ischaemia and reperfusion on NADH coenzyme Q reductase activity in rat liver
Q34449987Enzyme cytochemical techniques for metabolic mapping in living cells, with special reference to proteolysis
Q34063938Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses
Q45714040Glucose-6-phosphate dehydrogenase: the key to sex-related xenobiotic toxicity in hepatocytes of European flounder (Platichthys flesus L.)?
Q40459680Heterogeneity of kinetic parameters of enzymes in situ in rat liver lobules.
Q71668536Hypoxia-dependent changes of enzyme activities in different fibre types of rat soleus and extensor digitorum longus muscles. A cytophotometrical study
Q51573741In situ measurement of glutamate concentrations in the periportal, intermediate, and pericentral zones of rat liver.
Q36827938Kupffer cells and pit cells are not effective in the defense against experimentally induced colon carcinoma metastasis in rat liver
Q34221138Modulation of γ-secretase activity by multiple enzyme-substrate interactions: implications in pathogenesis of Alzheimer's disease
Q39435324Quantitative assessment of primary cerium reaction product formed by glucose-6-phosphatase activity in a male rat liver: an image analysis study
Q43678780Quantitative cytochemical analysis of glucose-6-phosphate dehydrogenase activity in living isolated hepatocytes of European flounder for rapid analysis of xenobiotic effects
Q71343738Quantitative enzyme- and immunohistochemistry of hexokinase and cytochrome c oxidase of spinal neurons in the zebrafish
Q44044270Sex-specific biotransformation and detoxification after xenobiotic exposure of primary cultured hepatocytes of European flounder (Platichthys flesus L.).
Q36865584The contribution of quantitative confocal laser scanning microscopy in cartilage research: chondrocyte insulin-like growth factor-1 receptors in health and pathology
Q50993407The dynamics of local kinetic parameters of glutamate dehydrogenase in rat liver.
Q35795820Yeast mitochondrial interactosome model: metabolon membrane proteins complex involved in the channeling of ADP/ATP