Poly(A) polymerase purified from HeLa cell nuclear extract is required for both cleavage and polyadenylation of pre-mRNA in vitro

scientific article published on January 1989

Poly(A) polymerase purified from HeLa cell nuclear extract is required for both cleavage and polyadenylation of pre-mRNA in vitro is …
instance of (P31):
scholarly articleQ13442814

External links are
P356DOI10.1128/MCB.9.1.193
P932PMC publication ID362161
P698PubMed publication ID2538718

P2093author name stringKeller W
Christofori G
P2860cites workSeparation of multiple components of HeLa cell nuclear extracts required for pre-messenger RNA splicing.Q54395405
Poly(A) site cleavage in a HeLa nuclear extract is dependent on downstream sequences.Q55060176
Alpha-thalassaemia caused by a polyadenylation signal mutation.Q55062701
Inhibition of RNA cleavage but not polyadenylation by a point mutation in mRNA 3′ consensus sequence AAUAAAQ58451156
A sequence downstream of AAUAAA is required for rabbit β-globin mRNA 3′-end formationQ59058833
3' cleavage and polyadenylation of mRNA precursors in vitro requires a poly(A) polymerase, a cleavage factor, and a snRNPQ64379456
Multiple factors are required for specific RNA cleavage at a poly(A) addition siteQ64379503
Electrophoretic separation of polyadenylation-specific complexesQ64379710
A small nuclear ribonucleoprotein associates with the AAUAAA polyadenylation signal in vitroQ64380127
Stepwise assembly of a pre-mRNA splicing complex requires U-snRNPs and specific intron sequencesQ64380337
Requirement of a downstream sequence for generation of a poly(A) addition siteQ64380598
Site-specific polyadenylation in a cell-free reactionQ64380699
Splicing of in vitro synthesized messenger RNA precursors in HeLa cell extractsQ64380855
Multiple factors are required for poly(A) addition to a mRNA 3' endQ68413214
Separation and characterization of a poly(A) polymerase and a cleavage/specificity factor required for pre-mRNA polyadenylationQ69820089
A functionally redundant downstream sequence in SV40 late pre-mRNA is required for mRNA 3'-end formation and for assembly of a precleavage complex in vitroQ69823612
Role of the Conserved AAUAAA Sequence: Four AAUAAA Point Mutants Prevent Messenger RNA 3′ End FormationQ72749074
The 5′ terminus of the RNA moiety of U1 small nuclear ribonucleoprotein particles is required for the splicing of messenger RNA precursorsQ72821863
Specific contacts between mammalian U7 snRNA and histone precursor RNA are indispensable for the in vitro 3' RNA processing reactionQ24564263
Structural and functional characterization of mouse U7 small nuclear RNA active in 3' processing of histone pre-mRNAQ24632127
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingQ25938984
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nucleiQ27860728
3' non-coding region sequences in eukaryotic messenger RNAQ27860858
Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoterQ27861016
Identification of the human U7 snRNP as one of several factors involved in the 3' end maturation of histone premessenger RNA'sQ28280579
U1, U2, and U4/U6 small nuclear ribonucleoproteins are required for in vitro splicing but not polyadenylationQ28302647
Accurate cleavage and polyadenylation of exogenous RNA substrateQ28646786
Transcription termination and 3' processing: the end is in site!Q29618288
Cleavage and polyadenylation of messenger RNA precursors in vitro occurs within large and specific 3' processing complexes.Q33930140
The conserved CAAGAAAGA spacer sequence is an essential element for the formation of 3' termini of the sea urchin H3 histone mRNA by RNA processingQ33930981
Fractionation of HeLa cell nuclear extracts reveals minor small nuclear ribonucleoprotein particlesQ34370740
Simple, efficientin vitrosynthesis of capped RNA useful for direct expression of cloned eukaryoti genesQ35716200
Two proteins crosslinked to RNA containing the adenovirus L3 poly(A) site require the AAUAAA sequence for bindingQ35989261
The consensus sequence YGTGTTYY located downstream from the AATAAA signal is required for efficient formation of mRNA 3' terminiQ36136653
Sedimentation analysis of polyadenylation-specific complexesQ36780246
Mutations in poly(A) site downstream elements affect in vitro cleavage activityQ36787633
Assembly of a polyadenylation-specific 25S ribonucleoprotein complex in vitroQ36788795
Requirements for accurate and efficient mRNA 3' end cleavage and polyadenylation of a simian virus 40 early pre-RNA in vitroQ36834250
Products of in vitro cleavage and polyadenylation of simian virus 40 late pre-mRNAsQ36838958
RNA sequence containing hexanucleotide AAUAAA directs efficient mRNA polyadenylation in vitroQ36885633
Identification of a sequence element on the 3' side of AAUAAA which is necessary for simian virus 40 late mRNA 3'-end processing.Q36894844
The AAUAAA sequence is required both for cleavage and for polyadenylation of simian virus 40 pre-mRNA in vitroQ36916774
Identification of a complex associated with processing and polyadenylation in vitro of herpes simplex virus type 1 thymidine kinase precursor RNAQ36921323
A sequence downstream of A-A-U-A-A-A is required for formation of simian virus 40 late mRNA 3' termini in frog oocytesQ37691120
The role of small nuclear ribonucleoprotein particles in pre-mRNA splicingQ39481136
In vitro cleavage of the simian virus 40 early polyadenylation site adjacent to a required downstream TG sequenceQ40668071
Formation of mRNA 3' termini: stability and dissociation of a complex involving the AAUAAA sequenceQ41330964
Analysis of mRNA 3' end formation by modification interference: the only modifications which prevent processing lie in AAUAAA and the poly(A) siteQ41360804
Specific pre-cleavage and post-cleavage complexes involved in the formation of SV40 late mRNA 3' termini in vitroQ41360882
Purification of a protein required for the splicing of pre-mRNA and its separation from the lariat debranching enzymeQ41432590
A 64 kd nuclear protein binds to RNA segments that include the AAUAAA polyadenylation motifQ41862487
Analysis of RNA cleavage at the adenovirus-2 L3 polyadenylation siteQ42572169
Isolation and partial characterization of the poly(A) polymerases from HeLa cells infected with vaccinia virusQ44372041
The sequence 5'-AAUAAA-3'forms parts of the recognition site for polyadenylation of late SV40 mRNAsQ48410195
P433issue1
P407language of work or nameEnglishQ1860
P921main subjectHeLaQ847482
P304page(s)193-203
P577publication date1989-01-01
P1433published inMolecular and Cellular BiologyQ3319478
P1476titlePoly(A) polymerase purified from HeLa cell nuclear extract is required for both cleavage and polyadenylation of pre-mRNA in vitro
P478volume9

Reverse relations

cites work (P2860)
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