Mutants of the EcoRI endonuclease with promiscuous substrate specificity implicate residues involved in substrate recognition

scientific article published on October 1990

Mutants of the EcoRI endonuclease with promiscuous substrate specificity implicate residues involved in substrate recognition is …
instance of (P31):
scholarly articleQ13442814

External links are
P932PMC publication ID552075
P698PubMed publication ID2209548

P2093author name stringHeitman J
Model P
P2860cites workProtein-DNA recognitionQ22065421
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DNA site recognition and overmethylation by the Eco RI methylaseQ67246121
Glu-111 is required for activation of the DNA cleavage center of EcoRI endonucleaseQ69652278
The negative charge of Glu-111 is required to activate the cleavage center of EcoRI endonucleaseQ69652281
Effects of functional group changes in the EcoRI recognition site on the cleavage reaction catalyzed by the endonucleaseQ69815944
The ability of the restriction endonuclease EcoRI to digest hemi-methylated versus fully cytosine-methylated DNA of the herpes tk promoter regionQ69852839
Replacement of potassium chloride by potassium glutamate dramatically enhances protein-DNA interactions in vitroQ69903975
Facilitated diffusion during catalysis by EcoRI endonuclease. Nonspecific interactions in EcoRI catalysisQ70105786
Coordinate ion pair formation between EcoRI endonuclease and DNAQ70175973
Base sequence and helix structure variation in B and A DNAQ71743861
Isolation of gram quantities of EcoRI restriction and modification enzymes from an overproducing strainQ72390025
DNA site recognition and reduced specificity of the Eco RI endonucleaseQ72872412
"Ultraviolet footprinting" accurately maps sequence-specific contacts and DNA kinking in the EcoRI endonuclease-DNA complexQ33642715
Repair of the Escherichia coli chromosome after in vivo scission by the EcoRI endonucleaseQ33847038
Determinants of EcoRI endonuclease sequence discriminationQ33856611
Restriction endonucleasesQ34325231
Specificity of substrate recognition by the EcoRI restriction endonucleaseQ34657231
The TaqI 'star' reaction: strand preferences reveal hydrogen-bond donor and acceptor sites in canonical sequence recognitionQ34681550
Relaxation of recognition sequence of specific endonuclease HindIII.Q34684351
Structure of the DNA-Eco RI endonuclease recognition complex at 3 A resolutionQ34684532
Alteration of the specificity of restriction endonucle, ases in the presence of organic solventsQ34708028
Cloning and Sequencing of Restriction Fragments Generated byEcoRI*Q34708343
The DNA loop model for ara repression: AraC protein occupies the proposed loop sites in vivo and repression-negative mutations lie in these same sitesQ35607430
Comparison of the nucleotide and amino acid sequences of the RsrI and EcoRI restriction endonucleasesQ35949088
Restriction endonuclease RsrI from Rhodobacter sphaeroides, an isoschizomer of EcoRI: purification and propertiesQ36063651
Site-specific methylases induce the SOS DNA repair response in Escherichia coliQ36240244
Involvement of outside DNA sequences in the major kinetic path by which EcoRI endonuclease locates and leaves its recognition sequenceQ36301554
Positive-selection cloning vehicle useful for overproduction of hybrid proteinsQ36329433
DNA-damaging agents stimulate gene expression at specific loci in Escherichia coliQ36389606
DNA nucleotide sequence restricted by the RI endonucleaseQ37501105
Accuracy of the EcoRI restriction endonuclease: binding and cleavage studies with oligodeoxynucleotide substrates containing degenerate recognition sequencesQ38340841
Role of the hydrophobic effect in stability of site-specific protein-DNA complexesQ38343324
Changing the hydrogen-bonding potential in the DNA binding site of EcoRI by site-directed mutagenesis drastically reduces the enzymatic activity, not, however, the preference of this restriction endonuclease for cleavage within the site-GAATTC-.Q38344996
Genetic engineering of EcoRI mutants with altered amino acid residues in the DNA binding site: physicochemical investigations give evidence for an altered monomer/dimer equilibrium for the Gln144Lys145 and Gln144Lys145Lys200 mutantsQ38345000
The effects of base analogue substitutions on the cleavage by the EcoRI restriction endonuclease of octadeoxyribonucleotides containing modified EcoRI recognition sequencesQ38350857
The enfolding arms of EcoRI endonuclease: role in DNA binding and cleavageQ38350968
Kinked DNA in crystalline complex with EcoRI endonucleaseQ38353764
Overmethylation of DNAs by the EcoRI methylaseQ39186545
Zinc fingers: gilt by associationQ39601107
Altering the specificity of restriction endonuclease: effect of replacing Mg2+with Mn2+Q40116305
Nucleotide sequence of bacteriophage f1 DNAQ40142434
Site directed mutagenesis experiments suggest that Glu 111, Glu 144 and Arg 145 are essential for endonucleolytic activity of EcoRI.Q40419671
Cassettes of the f1 intergenic region.Q40450399
The nucleotide sequence of pACYC177.Q40536520
DNA curvature in native and modified EcoRI recognition sites and possible influence upon the endonuclease cleavage reactionQ41942257
Dominant mutators in Escherichia coliQ42036991
The EcoRI restriction endonuclease with bacteriophage lambda DNA. Equilibrium binding studiesQ42881811
Isolation and analysis of arc repressor mutants: evidence for an unusual mechanism of DNA bindingQ45141440
Dissecting the catalytic triad of a serine proteaseQ46366429
Clustering of null mutations in the EcoRI endonucleaseQ46978001
P433issue10
P407language of work or nameEnglishQ1860
P304page(s)3369-3378
P577publication date1990-10-01
P1433published inThe EMBO JournalQ1278554
P1476titleMutants of the EcoRI endonuclease with promiscuous substrate specificity implicate residues involved in substrate recognition
P478volume9

Reverse relations

cites work (P2860)
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