The 'endo-blue method' for direct cloning of restriction endonuclease genes in E. coli.

scientific article published on June 1994

The 'endo-blue method' for direct cloning of restriction endonuclease genes in E. coli. is …
instance of (P31):
scholarly articleQ13442814

External links are
P356DOI10.1093/NAR/22.12.2399
P932PMC publication ID523701
P698PubMed publication ID8036170
P5875ResearchGate publication ID15163417

P50authorElisabeth A. RaleighQ43149059
P2093author name stringS Y Xu
J P Xiao
A Fomenkov
D Dila
P2860cites workA rapid alkaline extraction procedure for screening recombinant plasmid DNAQ24614998
Cloned restriction-modification systems--a reviewQ28289745
Construction and characterization of new cloning vehicle. II. A multipurpose cloning systemQ28298407
Tn10 transposition promotes RecA-dependent induction of a lambda prophageQ33640569
Repair of the Escherichia coli chromosome after in vivo scission by the EcoRI endonucleaseQ33847038
Organization of restriction-modification systemsQ34111928
Cloning and expression of the Pst I restriction-modification system in Escherichia coliQ34252443
Organization and function of the mcrBC genes of Escherichia coli K‐12Q35228347
A new method for the rapid identification of genes encoding restriction and modification enzymesQ35761450
Characterization and expression of the Escherichia coli Mrr restriction systemQ36151697
Site-specific methylases induce the SOS DNA repair response in Escherichia coliQ36240244
DNA-damaging agents stimulate gene expression at specific loci in Escherichia coliQ36389606
Escherichia coli K-12 restricts DNA containing 5-methylcytosineQ37409547
Inducible DNA repair systemsQ39826678
Isolation of temperature-sensitive McrA and McrB mutations and complementation analysis of the McrBC region of Escherichia coli K-12.Q39938752
Cofactor requirements of BamHI mutant endonuclease E77K and its suppressor mutantsQ39942710
A novel activity in Escherichia coli K-12 that directs restriction of DNA modified at CG dinucleotidesQ39942806
Nucleotide sequence of the BsuRI restriction-modification system.Q40471693
On the origins, structures and functions of restriction-modification enzymes.Q40533719
Cloning of restriction and modification genes in E. coli: The HhaII system from Haemophilus haemolyticusQ40899342
Mutants of the EcoRI endonuclease with promiscuous substrate specificity implicate residues involved in substrate recognitionQ41227774
Substrate recognition by the EcoRI endonuclease.Q54720062
Restriction and modification in vivo by Escherichia coli K12.Q54771205
McrBC: a multisubunit GTP-dependent restriction endonucleaseQ58032884
Cloning the modification methylase gene of Bacillus sphaericus R in Escherichia coliQ60505834
SOS induction as an in vivo assay of enzyme-DNA interactionsQ67996221
An endonuclease specific for single-stranded DNA selectively damages the genomic DNA and induces the SOS responseQ70060861
P433issue12
P921main subjectEscherichia coliQ25419
P1104number of pages5
P304page(s)2399-2403
P577publication date1994-06-01
P1433published inNucleic Acids ResearchQ135122
P1476titleThe 'endo-blue method' for direct cloning of restriction endonuclease genes in E. coli
P478volume22

Reverse relations

cites work (P2860)
Q33283172A rapid and efficient method for cloning genes of type II restriction-modification systems by use of a killer plasmid.
Q39562182Characterization of an extremely thermostable restriction enzyme, PspGI, from a Pyrococcus strain and cloning of the PspGI restriction-modification system in Escherichia coli.
Q34416538Cloning and expression of AatII restriction-modification system in Escherichia coli
Q48058745Cloning and sequence comparison of AvaI and BsoBI restriction-modification systems
Q40637190Cloning of CviPII nicking and modification system from chlorella virus NYs-1 and application of Nt.CviPII in random DNA amplification
Q39721447Cloning of the BssHII restriction-modification system in Escherichia coli : BssHII methyltransferase contains circularly permuted cytosine-5 methyltransferase motifs
Q36686918Cloning of the ppu21IM gene using a in vivo selection method
Q43030612Directed evolution of restriction endonuclease BstYI to achieve increased substrate specificity
Q47683418Early Sex Identification in Date Palm by Male-Specific Sequence-Characterized Amplified Region (SCAR) Markers
Q42074741Engineering BspQI nicking enzymes and application of N.BspQI in DNA labeling and production of single-strand DNA.
Q25257169Engineering a rare-cutting restriction enzyme: genetic screening and selection of NotI variants
Q24649467Expression and purification of BmrI restriction endonuclease and its N-terminal cleavage domain variants
Q35622615Expression and purification of a single-chain Type IV restriction enzyme Eco94GmrSD and determination of its substrate preference
Q42965698Functional analysis of MmeI from methanol utilizer Methylophilus methylotrophus, a subtype IIC restriction-modification enzyme related to type I enzymes
Q40855229Genomic structure of the human DNA methyltransferase gene
Q41366977In vivo DNA protection by relaxed-specificity SinI DNA methyltransferase variants
Q38353346Isolation of BsoBI restriction endonuclease variants with altered substrate specificity
Q90297236Low level expression of the Type II restriction-modification system confers potent bacteriophage resistance in Escherichia coli
Q36497080Rational design of a chimeric endonuclease targeted to NotI recognition site
Q34323730Screening for catalytically active Type II restriction endonucleases using segregation-induced methylation deficiency
Q56267595Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element
Q31091708The isolation of strand-specific nicking endonucleases from a randomized SapI expression library
Q40001082Transposon-mediated linker insertion scanning mutagenesis of the Escherichia coli McrA endonuclease.
Q33177525Yeast and human genes that affect the Escherichia coli SOS response

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