Heterologous expression, purification, reconstitution and kinetic analysis of an extended type II polyketide synthase

scientific article published on September 1999

Heterologous expression, purification, reconstitution and kinetic analysis of an extended type II polyketide synthase is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1016/S1074-5521(99)80112-4
P8608Fatcat IDrelease_atybywlvofdc5hmsclpenkxfoe
P698PubMed publication ID10467128

P2093author name stringKhosla C
Zawada RJ
P2860cites workTolerance and specificity of polyketide synthasesQ28139420
MCAT is not required for in vitro polyketide synthesis in a minimal actinorhodin polyketide synthase from Streptomyces coelicolorQ28292215
A new enzyme superfamily - the phosphopantetheinyl transferasesQ28297964
Biosynthesis of anthraquinones by interspecies cloning of actinorhodin biosynthesis genes in streptomycetes: clarification of actinorhodin gene functionsQ28335942
The tcmVI region of the tetracenomycin C biosynthetic gene cluster of Streptomyces glaucescens encodes the tetracenomycin F1 monooxygenase, tetracenomycin F2 cyclase, and, most likely, a second cyclaseQ36124265
Molecular genetics of polyketides and its comparison to fatty acid biosynthesisQ37794703
Kinetic analysis of the actinorhodin aromatic polyketide synthaseQ41686123
Engineered biosynthesis of novel polyketides: stereochemical course of two reactions catalyzed by a polyketide synthaseQ42283765
Isolation and structural elucidation of tetracenomycin F2 and tetracenomycin F1: early intermediates in the biosynthesis of tetracenomycin C in Streptomyces glaucescensQ42286438
Engineered biosynthesis of novel polyketidesQ42286784
Cloning, sequencing and deduced functions of a cluster of Streptomyces genes probably encoding biosynthesis of the polyketide antibiotic frenolicinQ42605670
Purification and separation of holo- and apo-forms of Saccharopolyspora erythraea acyl-carrier protein released from recombinant Escherichia coli by freezing and thawingQ42811676
Purification and in vitro reconstitution of the essential protein components of an aromatic polyketide synthaseQ45114750
Rational design of aromatic polyketide natural products by recombinant assembly of enzymatic subunitsQ46151499
Domain analysis of the molecular recognition features of aromatic polyketide synthase subunitsQ46476885
Reconstitution of the iterative type II polyketide synthase for tetracenomycin F2 biosynthesisQ48019885
Ability of Streptomyces spp. acyl carrier proteins and coenzyme A analogs to serve as substrates in vitro for E. coli holo-ACP synthase.Q54574034
mutactinQ59115055
Engineered biosynthesis of novel polyketides: evidence for temporal, but not regiospecific, control of cyclization of an aromatic polyketide precursor.Q64967073
Enzymatic synthesis of a bacterial polyketide from acetyl and malonyl coenzyme AQ72628094
P433issue9
P304page(s)607-615
P577publication date1999-09-01
P1433published inChemistry and BiologyQ15758410
P1476titleHeterologous expression, purification, reconstitution and kinetic analysis of an extended type II polyketide synthase
P478volume6

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cites work (P2860)
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Q28545284Expanding our understanding of sequence-function relationships of type II polyketide biosynthetic gene clusters: bioinformatics-guided identification of Frankiamicin A from Frankia sp. EAN1pec
Q31142779Heterologous production of flavanones in Escherichia coli: potential for combinatorial biosynthesis of flavonoids in bacteria
Q35866989In Vitro Reconstitution of Metabolic Pathways: Insights into Nature's Chemical Logic
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Q38038812The structural role of the carrier protein--active controller or passive carrier
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